flow field flow fractionation analysis of biomolecules and their complexes

... analyses of single protein and simple protein complex samples were mainly conducted on MALDI-TOF, MALDI-TOF/TOF, ESI-TOF (electrospray ionization-TOF) and ESI-Q-TOF (ESI-linear trap quadrupole-TOF) ... complexes 42 44 2.2.1 The Pol II complex and the Pol II-TFIIF complex 44 2.2.2 Cross-linking titration of Pol II and Pol II-TFIIF complexes 45 2.2.3 Cross-linking of Pol II and Pol II-TFIIF complexes ... structure of proteins and protein complexes XIX Chapter INTRODUCTION 1.1 Integrated structural biology and 3D proteomics 1.1.1 Integrated structural analysis of large protein complexes and assemblies...

Ngày tải lên: 30/09/2015, 05:51

... Evaluation of FCM analysis of GR expression by western blot Analysis of GR repression in (left) K562 and (right) U937 with and without 10-6 M Dex of GR expression by western blot by GR-mAb FCM and western ... Page of 11 (page number not for citation purposes) Arthritis Research & Therapy Vol 11 No Du et al Figure Evaluation of FCM analysis of GR binding by RLBA Analysis of GR binding in (a) K562 and ... GR expression and binding (mean ± standard error of the mean) For heterogeneity of variance, Kruskal-Wallis H test and Memenyi test were used Age, gender, dosage of prednisolone and years since...

Ngày tải lên: 09/08/2014, 14:22

... on their effectiveness in reducing P and COD  Conduct and present a MFA of P and COD flows of future scenarios and compare the results with a business-as-usual (BAU) scenario 1.4 Limitation of ... biological and chemical treatment plant and redirecting all of the sludge and a majority of the food waste to Biogas production instead of to Composting and Anaerobic treatment The P and COD flows ... share of the P flow, with a total flow of 1.8 ton/year The largest share goes as waste to the Landfill Livestock and Compost receive the remainder of the food waste Surprisingly more than half of...

Ngày tải lên: 11/10/2016, 08:37

... 24.19), a slope of 4.91 and a correlation coefficient of 0.999 The analysis of some authentic samples of permanganate by this new method gave an average percentage recovery of 99.3% (Table and Fig 10) ... the best straight line has a slope of 2.01 and a correlation coefficient of 0.999 The results of the analysis of some IOÀ authen4 tic samples are shown in Table and Fig The found results agree reasonably ... calibration graphs and for the analysis of some authentic samples, proves that this proposed method is reasonably accurate, precise, simple and cheap Flow injection analysis of some oxidants using...

Ngày tải lên: 02/09/2015, 13:46

... investigation of numerous materials, components and systems previously impossible to measure by classical optical method The measurement of the changes of phase of the wavefield and thus the change of any ... precise results and bring forward a wealth of possible research directions vi NOMENCLATURE NOMENCLATURE E Electrical field form of light waves a Real of amplitude of light wave ϕ The phase of light ... A/Prof Quan Chenggen and A/Prof Tay Cho Jui for their advice and guidance throughout his research I would like to take this opportunity to express my appreciation for their constant support and...

Ngày tải lên: 03/10/2015, 21:55

... chuyển tiếp dị thể n-Si/p-CIS 14 CHƯƠNG CHƯƠNG TRÌNH MÔ PHỎNG MỘTCHIỀU AMPS – 1D (Analysis of Microelectronic and Photonic Structures) Chương trình mô chiều AMPS – 1D chương trình đa để khảo sát...

Ngày tải lên: 18/03/2013, 13:40

... CHEMICAL ANALYSIS OF WATER AND WASTEWATER 24.1 GENERAL ASPECTS OF ENVIRONMENTAL CHEMICAL ANALYSIS Scientists’ understanding of the environment can only be as good as their knowledge of ... of the identities and quantities of pollutants and other chemical species in water, air, soil, and biological systems Therefore, proven, state -of- the-art techniques of chemical analysis, properly ... in the evolution of analytical chemistry, characterized by the development of new and improved analysis techniques that enable detection of much lower levels of chemical species and a vastly increased...

Ngày tải lên: 23/07/2013, 20:57

... Definition of the energy analysis parameters The energy analysis and simulation of buildings requires the definition of a number of parameters, related to construction characteristics (geometry and ... Dioxide (CO2) footprint Table Energy and environmental indices per building type and year of construction Building Type Classrooms Offices Offices Offices Offices Offices Theatres Laboratories Construction ... Member of the Technical Chamber of Greece, and Member of the Union of Mechanical and Electrical Engineers of Northern Greece E-mail address: vvoulgar@gmail.com Nikolas Kyriakis is the Director of...

Ngày tải lên: 05/09/2013, 16:10

... Idealism: Negotiation and Administration in Hegel’s Account of the Structure and Content of Conceptual Norms,” European Journal of Philosophy, () (August ), –, and Tales of the Mighty Dead, ... of the world-process of things coming-to-be and passing-away taken as a whole This world-process of coming-to-be and passing-away is thus all that there is, and it is within this conception of ... shared some of its inspiration The most extensive comparison and critique of Hegel’s Logic from the standpoint of Fregean and post-Fregean formal logic is to be found in Pirmin Stekeler-Weithofer,...

Ngày tải lên: 01/11/2013, 08:20

... constituent parts interrelate and interact together in a kind of discourse 21 - Comparative and contrastive methods: The results of analytic and synthetic data analysis of opening and closing speeches ... improve their professional public speaking skills and the Vietnamese learners of English who are scared of public speaking confidently perform their public speaking as well as better their presentation ... English and Vietnamese in terms of their discourse features? Closing speeches: - What are the discourse features of MC closing speeches in American English and Vietnamese in terms of their layout,...

Ngày tải lên: 26/11/2013, 12:41

... features of EAPs and VAPs in terms of their layout, lexical features, syntactic features and cohesive devices? What are the similarities and differences between EAPs and VAPs in terms of their layout, ... beauty of language, strategies and techniques through the use of layout, syntax, lexis and devices that are expected to be beneficial For the above reasons, “A Discourse Analysis of English and ... Objectives of the Study - To identify some discourse features of EAPs and VAPs in terms of their layout, lexical features, syntactic features and cohesive devices - To find out the similarities and...

Ngày tải lên: 26/11/2013, 13:26

... understand their cases of Secondly, English considers the WDB “owl” as the symbol meaning, it is important to consider them in their collocations From of “wisdom” on the basis of their experience and ... teaching and learning of English 3 1.4 RESEARCH QUESTIONS: (1) What are the similarities and differences of semantic features of the WDBs in English and Vietnamese? (2) What are the similarities and ... Semantic Qu crow Table 4.2: The semantic fields of the WDBs in English and N Gà non Cu c devices of metaphor, metonymy and simile in their collocation and Gà M t gà m Cò L cò b Gà Ch i Tiredness...

Ngày tải lên: 26/11/2013, 13:30

... understand and use WIRHs in English and Vietnamese, learners should be equipped with not only grammatical meaning and descriptive meanings of WIRHs, but also their components of meaning and their ... similarities and differences between WIRHs in So far there has been no contrastive analysis of grammatical English and Vietnamese in terms of grammatical features? and semantic features of words and idioms ... the grammatical and semantic features of some verbs learners, users or translators of English often meet a lot of difficulties and idioms related to hearing (WIRHs) in English and Vietnamese in...

Ngày tải lên: 26/11/2013, 13:31

... terms of Noun, Verb, Adjective and Adverb The third step is to discuss the result of analysis and giving explanations for the similarities and differences in terms of grammatical, semantic and ... the meaning of the words denoting men and women’s characteristics from the initial investigation and the ability to use these words today The concept of WsDMC and their linguistic and sociocultural ... features of the WsDMC in English and Vietnamese - Scores of similarities and differences between the sociocultural features of WsDMC in English and Vietnamese From which we draw conclusions and generalize...

Ngày tải lên: 26/11/2013, 13:31

... about Theme and Rheme, although "The Analysis of Theme and Rheme in "Little House on the Prairie" is shown as the theme of the study Method of the Study - A great number of documents and materials ... out of the context Moreover, the common traditional practice of language teaching and learning has been on the analysis of single sentences, normally at the levels of phonology, vocabulary, and ... "Little House on the Prairie" as the source of data to the study 2.4 Principles of Analysis In our study, a linguistic description and analysis of Theme and Rheme is carried out Since the study...

Ngày tải lên: 22/12/2013, 12:59

... program The minimum requirements of this program consist of an initial demonstration of laboratory capability and an ongoing analysis of spiked samples to evaluate and document data quality The ... bottles and, if residual chlorine is present, add 80 mg of sodium thiosulfate per liter of sample and mix well EPA Methods 330.4 and 330.5 may be used for measurement of residual chlorine.9 Field ... ASTM Annual Book of Standards, Part 31, D3694-78 “Standard Practices for Preparation of Sample Containers and for Preservation of Organic Constituents,” American Society for Testing and Materials,...

Ngày tải lên: 14/02/2014, 03:20

... and 6.7 It is recommended that the secondary dilution standard be prepared at a concentration of 15 µg/mL of each internal standard compound The addition of 10 µL of this standard to 5.0 mL of ... program The minimum requirements of this program consist of an initial demonstration of laboratory capability and an ongoing analysis of spiked samples to evaluate and document data quality The ... concentration of the stock standard Optionally, stock standard solutions may be prepared using the pure standard material by volumetrically measuring the appropriate amounts and determining the weight of...

Ngày tải lên: 14/02/2014, 03:20

... apoptosis and induces the synthesis of antioxidants [22] Alterations in mitochondrial proteins, on the other hand, are specifically linked to one of the major pathogenetic mechanisms of PD [5] Worthy of ... in all gels of each experimental condition were built and then compared; spots were quantified on the basis of their relative volume (spot volume normalized to the sum of the volumes of all the ... levels of a-synuclein and HSP70 were calculated by densitometric analysis (imagej software; http://rsb.info.nih.gov/ij) and normalized to b-actin All experiments were run in triplicate Proteomics of...

Ngày tải lên: 15/02/2014, 01:20

... analysis of peptides and proteins N D Udeshi et al dimethylation of Arg, monomethylation, dimethylation and trimethylation of Lys, acetylation and ubiquitination of Lys, and phosphorylation of ... higher than predicted Further analysis of c¢-type ions (singly and doubly ETD-MS analysis of peptides and proteins charged) indicates that the Tyr residues at positions 31 and 40 are both 80 mass units ... pair of fragment ions of type c¢ and z¢Æ Subtraction of the m ⁄ z values for the fragments within a given ion series that differ by a single amino acid affords the mass and thus the identity of...

Ngày tải lên: 18/02/2014, 16:20

... panels A and B and the relative amounts of each of the subunits determined by densitometry scanning of the stained Western blots of the q° and CKL57 membranes are shown in panels C and D, respectively ... resuspended in mL of MTE buffer, divided in aliquots of 50 lL each, and stored at )80 °C for subsequent analysis by SDS/PAGE and Western blotting Isolation of mitochondrial membranes SDS/PAGE and Western ... levels of all three catalytic subunits, cytochrome b, ISP and cytochrome c1 In addition, core proteins and and subunit were reduced to about half of their original levels, while subunits and were...

Ngày tải lên: 19/02/2014, 12:20