ezh2 and nf κb crosstalk in luminal breast cancer
Báo cáo y học: "Dysregulated apoptosis and NFκB expression in COPD subjects" pptx
... subunits. In unstim- ulated cells NF? ?B is sequestered in the cytoplasm due to its binding to IκBα and IκBβ. Following cell stimulation, IκB is rapidly phosphorylated by specific protein kinases leading ... subjects and the potential involvement of NF? ?B in this process we used three methods to investigate neutrophil apoptosis in COPD and determine any associated increase in NF? ?B activation in airway ... the inhibition of NF? ?B inducing apoptosis in murine B cells [44], and in other cell types, activation of NF? ?B seems to correlate with Percent of apoptotic neutrophils in sputum by DNA strand...
Ngày tải lên: 12/08/2014, 14:20
Viola yedoensis suppresses cell invasion by targeting the protease and NF-κB activities in A549 and lewis lung carcinoma cells
... C-glycosides, and O-glycosides), dicoumarin (including dimeresculetin, euphorbetin, and esculetin) and cyclotides [10, 13, 14] V yedoensis inhibits β-hexosaminidase and histamine release and down-regulates ... Matrix metalloproteinases (MMPs) and serine proteinase play a key role in cancer metastasis [3], particularly in damaging the ECM MMP-9 (gelatinase B, 92 kDa) and MMP-2 (gelatinase A, 72 kDa) are ... expression of inflammatory cytokines (such as IL-1β, TNF-α, IL-6, and iNOS) to block the inflammatory development in RBL-2H3 mast cells [15] Nevertheless, the anti -cancer effect of V yedoensis in human...
Ngày tải lên: 14/01/2020, 22:58
Expression and significance of HMGB1, TLR4 and NF-κB p65 in human epidermal tumors
... acts as a novel proinflammatory cytokine which interacts with Toll like receptor (TLR4) to activate nuclear factor -κB (NF- κB) This sequence of events is involved in tumor growth and progression However, ... and Feili Gong1* Abstract Background: High mobility group protein box (HMGB1) is a DNA binding protein located in nucleus It is released into extracellular fluid where it acts as a novel proinflammatory ... and NF- κB on epidermal tumors remain unclear Methods: Human epidermal tumor specimens were obtained from 96 patients Immunohistochemistry was used to detect expression of HMGB1, TLR4 and NF- κB...
Ngày tải lên: 05/11/2020, 06:47
gbe50 attenuates inflammatory response by inhibiting the p38 mapk and nf b pathways in lps stimulated microglial cells
... proinflammatory cytokines, including IL-1𝛽 and IL6, in hippocampal cells [12, 18] Microglia are a major type of inflammatory cells in the CNS [19, 20], which exist mainly in two forms: resting and ... (including kaempferol, quercetin, and isorhamnetin derivatives) and 6.4% lactones (including ginkgolides A, B, C, and J and bilobalide), and the adverse ingredient ginkgo acid content is smaller ... the NF- 𝜅B p65 and p38 MAPK pathways was involved in the production of proinflammatory cytokines in activated microglia Consistent with the involvement of the NF- 𝜅B p65 and p38 MAPK pathways in...
Ngày tải lên: 02/11/2022, 10:41
Parkin gene mutations are not common, but its epigenetic inactivation is a frequent event and predicts poor survival in advanced breast cancer patients
... of Parkin mutations in breast cancer were obtained Oncomine database and UALCAN analysis Oncomine database was exploited to investigate the mRNA expression of Parkin in breast cancer using the ... of Parkin (PARK-2) expression in different types of breast carcinoma in TCGA a Invasive lobular breast carcinoma vs normal tissues, b Invasive breast carcinoma vs normal tissues, and c Invasive ... Parkin protein in breast tissue at 100X and 400X magnifications (i) Parkin positive expression in normal tissue, (ii) Parkin negative expression, (iii) Parkin positive expression in breast carcinoma...
Ngày tải lên: 17/06/2020, 17:24
Aberrant promoter methylation of YAP gene and its subsequent downregulation in indian breast cancer patients
... sporadic breast cancer cases were included in the study Normal adjacent breast tissue not infiltrated with tumor served as control Inclusion criteria included female breast cancer patients in the ... protein (Fig and Table 5) and the percentage of YAP protein downregulation (64.81%) was higher in advanced stages III and IV of breast cancer The percentage of YAP protein downregulation in breast ... Figure S1A) YAP coding strand had 40.00% C > T and 60.0% G > A mutation in breast cancer YAP TCGA database in human breast cancer Researchers are provided with huge genome and clinical data through...
Ngày tải lên: 24/07/2020, 01:52
Methylene blue photodynamic therapy induces selective and massive cell death in human breast cancer cells
... plated and maintained in control conditions or exposed to MB-PDT and then stained with the DNA-binding dyes Propidium iodide (PI, SigmaAldrich) and Hoechst 33342 (HO, Sigma-Aldrich) for 10 Following ... cells Intracellular methylene blue quantification × 105 cells/cm2 were plated and incubated with mL of medium containing MB (20 μM) and maintained for 1, 2, 4, and hours At each time point, the ... The incorporation of MB was determined by correcting the absorbance of MB by the number of cells remaining in each well after the incubation period Intracellular singlet oxygen generation Singlet...
Ngày tải lên: 19/09/2020, 21:32
The prognostic value of stromal and epithelial periostin expression in human breast cancer: Correlation with clinical pathological features and mortality outcome
... that maintain a strong independent predictive value of both all causes and, namely, of cancer- specific mortality Keywords: Human periostin protein, Breast neoplasms, Extracellular matrix proteins, ... IRCCS AOU San Martino-IST, San Martino University Hospital and National Cancer Research Institute, L.go R Benzi 10, 16132 Genoa, Italy Department of Internal Medicine, School of Medicine, University ... phenotype and the risk of mortality following surgery were analysed Appropriate statistics, including the Fine and Gray competing risk proportional hazard regression model, were used (Continued on...
Ngày tải lên: 21/09/2020, 11:39
TIMP-1 and responsiveness to gemcitabine in advanced breast cancer; results from a randomized phase III trial from the Danish breast cancer cooperative group
... taxane disrupting the dynamic function of microtubules [22], and gemcitabine (G), a pyrimidine analog arresting DNA replication and synthesis [23-25], are widely used in breast cancer therapy ... raised against recombinant human TIMP-1 [30,31] was applied for immunohistochemical (IHC) staining as previously described [8] In brief, sections were deparaffinized in xylene and rehydrated in graded ... (no staining of tumor cells) staining signal as a measure of the TIMP-1 immunoreactivity in the epithelial breast cancer cells [2,8,20] The whole-tissue sections were scanned and examined by...
Ngày tải lên: 05/11/2020, 00:32
High levels of γ-glutamyl hydrolase (GGH) are associated with poor prognosis and unfavorable clinical outcomes in invasive breast cancer
... negative In order to distinguish positive from negative staining as for ER and Her2/neu staining, the immunoreactivity was considered as positive, indicating weak, moderate or strong staining in more ... of breast tumors is mirrored in the identification of at least five molecular subtypes including basal-like, luminal A, luminal B, HER2-enriched and normal-like, which are believed to originate ... in the development and progression of invasive breast cancer, warranting further investigations Our findings suggest that GGH serve as a potential biomarker of unfavorable clinical outcomes over...
Ngày tải lên: 05/11/2020, 07:05
Gonadotropin-releasing hormone receptor activates GTPase RhoA and inhibits cell invasion in the breast cancer cell line MDA-MB-231
... cell proliferation and tumor invasiveness [15-18] In fact, GnRH and some of its agonists have shown to be effective in controlling tumor growth and invasiveness in in vitro and in vivo systems [19-21] ... whereby GnRH inhibits breast cancer cell invasion Methods: Actin polymerization and substrate adhesion was measured in the highly invasive cell line, MDA-MB-231 transiently expressing the wild-type ... of breast cancer tumors as well as several breast cancer- derived cell lines express specific binding sites for GnRH [26,27] The role of GnRH and GnRH agonists (GnRHa) to inhibit growth of breast...
Ngày tải lên: 05/11/2020, 07:51
Cyclic stretch enhances the expression of Toll-like Receptor 4 gene in cultured cardiomyocytes via p38 MAP kinase and NF-B pathway doc
... DNA-protein binding activ- ity induced by stretch (Fig. 6A). Our fin ding indicates that Ang II receptor-related mechanism is involved in cyclic stretch-induced NF- B activity. DNA-binding complexes induced ... phosphory- lation of NF- B induced by stretch (Fig. 6B and 6C), indicating Ang II and T NF- a receptors are involved in cyclic stretch-induced NF- B phosphorylation. Cyclic stretch increases TLR4 promoter ... Figure 6 Cyclic stretch increases NF- B-bindi ng activity and NF- B protein phosphorylation. (A) Representative EMSA showing protein binding to the NF- B oligonucleotide in nuclear extracts of...
Ngày tải lên: 10/08/2014, 05:21
Báo cáo y học: " Mitogen-activated protein kinases and NFκB are involved in SP-A-enhanced responses of macrophages to " pdf
... increased activation of intra-macrophage signalling pathways involving MAP kinases and NF? ?B We have examined the role of both the MAPK pathway and NF? ?B activation in BCG killing and nitric oxide production ... kinases in controlling microbial infection are the findings that pathogenic strains of various bacteria block inflammatory mediator production through inhibition of MAP kinases [21-23] Following ... we have investigated the role of tyrosine kinases and the downstream mitogenactivated protein kinase (MAPK) family, and the transcription factor NF? ?B in mediating the enhanced signaling in response...
Ngày tải lên: 12/08/2014, 14:20
Selective activation of TNFR1 and NF-κB inhibition by a novel biyouyanagin analogue promotes apoptosis in acute leukemia cells
... latter, NF- κB promotes the transcription of genes encoding proteins involved in the suppression of cell death by both the intrinsic and the extrinsic pathway [3, 4] Thus, elevation in NF- κB activity ... human TNFα mainly in advanced solid cancers [20, 21] Recombinant human TNFα (rhTNFα) has been tested as a systemic treatment in several clinical trials and used as both a single agent and in Page ... characterized by an increase in membrane-associated TNFR1, activation of Caspase-8 and proteolytic inactivation of the death domain kinase RIP1 indicating that KC-53 induced mainly the extrinsic/death...
Ngày tải lên: 21/09/2020, 09:54
Aberrant activation of NF-κB signaling in mammary epithelium leads to abnormal growth and ductal carcinoma in situ
... results in significant macrophage infiltration into the ducts These immune cells are likely acting in an “M1” role initially, responding to the inflammatory signals as they would to an infection ... NF- κB hyper-activation can lead to pre-malignant changes in the breast Similar changes in architecture and molecular signaling could be occurring in human breast tissue after acute infection, injury, ... elements in DNA Via CHIP analysis, it was shown that NF- κB bound the p18INK4c promoter, which contains these Alu-repeats, following viral infection of Hela cells [39] Continued studies of how NF- κB...
Ngày tải lên: 28/09/2020, 01:40
St Gallen molecular subtypes in primary breast cancer and matched lymph node metastases - aspects on distribution and prognosis for patients with luminal A tumours: Results from a
... assessing them individually [6-8] In 2011, the St Gallen International Breast Cancer Conference suggested a surrogate definition of intrinsic subtypes of breast cancer: luminal A (ER + and/ or ... constructed according to the St Gallen International Breast Cancer Conference 2011 [10]: luminal A (ER + and/ or PR+, Ki67 low and HER2-), luminal B (ER + and/ or PR+, Ki67 high and/ or HER2+), HER2-positive ... aimed to investigate whether classification into luminal A, luminal B, HER2-positive and triple-negative subtypes provides information beyond that of the individual analyses of ER, PR, HER2 and Ki67...
Ngày tải lên: 05/11/2020, 05:39
glucose 6 phosphate dehydrogenase knockdown enhances il 8 expression in hepg2 cells via oxidative stress and nf b signaling pathway
... (TNF-α) induced -NF- κB, whereas the antioxidant N-acetyl-L-cysteine (NAC) suppresses TNF-α induced -NF- κB [40] G6PD can activate NF- κB in different cell types, including adipocytes in obesity and ... blockade of NF- κB due to selective inhibitor or specific SiRNA against NF- κB confirms that the up-regulation of IL-8 caused by G6PD deficiency is linked to NF- κB signaling Nevertheless, the finding that ... response [12] During inflammation, the production of intracellular reactive oxygen species (ROS) is involved in triggering inflammatory responses via the secretion of pro-inflammatory cytokines, which...
Ngày tải lên: 02/11/2022, 10:43
Xanthatin induces cell cycle arrest at g2 m checkpoint and apoptosis via disrupting nf b pathway in a549 non small cell lung cancer cells
... xanthatin inhibited NF- κB signaling in A549 cells by suppressing NF- κB (p65) translocation Figure Xanthatin disrupted NF- κB signaling in A549 cells; (A) Protein expression of p-IκB, IκB, p -NF- κB ... with many cancer types including lung cancer [27] Thus to further gain insights into the mechanisms of xanthatin-induced apoptosis, we investigated the expressions of key proteins in NF- κB pathway ... xanthatin a more valuable candidate for cancer chemotherapy given that invasion and metastasis of tumor cell are closely related to inflammation, while NF- κB is indeed a key player in inflammation-induced...
Ngày tải lên: 15/03/2023, 20:11
Application of a human bone engineering platform to an in vitro and in vivo breast cancer metastasis model
... Bone Engineering Platform to an In Vitro and In Vivo Breast Cancer Metastasis Model Verena Maria Charlotte Reichert, MD Faculty of Built Environment and Engineering, School of Engineering Systems, ... Osteomimicry of breast cancer cells 26 2.9 Osteolytic bone metastasis 27 2.10 Models investigating bone -cancer cell interaction in vitro 29 2.11 Models investigating bone -cancer cell interaction ... human breast cancer cells and a brain-seeking clone in vivo and in vitro J Bone Miner Res, 2001 16(8): p 1486-95 Ross, D.T and C.M Perou, A comparison of gene expression signatures from breast...
Ngày tải lên: 07/08/2017, 15:52
The contribution of large genomic rearrangements in BRCA1 and BRCA2 to South African familial breast cancer
... multi-exon CNVs involving exons 1a–2 and exons 4–6 deletion, each includes a critical domain, namely the initiation site for protein translation Met1 and the RING finger binding domain, which is required ... located in BRCA2 exon 17 [17] The position of these SNVs influenced the binding and ligation of the probes (data not shown) These false positive findings were not observed in any of the confirmation ... counselling and written informed consent was obtained prior inclusion The research conformed to the provisions of the Declaration of Helsinki in 2013 Consent for publication Written consent was obtained...
Ngày tải lên: 30/05/2020, 21:37
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