expression regulation of retroviral gag and rna

... decreased the expression of HO-2 mRNA and protein and induced the expression of HO-1 mRNA and protein In contrast, the scrambled HO-2 siRNA did not affect the expression of HO-1 and HO-2 mRNAs and proteins ... HO-2 mRNA with HO-2 siRNA induced the expression of HO-1 mRNA and protein in HepG2 cells, but did not change the concentrations of GAPDH mRNA and b-actin (Fig 4C,D) Consistent with HO-1 mRNA expression, ... resetting of HO-1 expression In contrast with HO-1 expression, expression of HO-2 mRNA and protein was not increased in several human cell lines examined [15,19,37] Such a mode of regulation of HO-2 expression...

Ngày tải lên: 19/02/2014, 05:20

... to FEBS Journal 275 (2008) 4571–4582 ª 2008 The Authors Journal compilation ª 2008 FEBS 4575 Internalization of cystatin C U Ekstrom et al ¨ Fig ELISA and western blotting of internalized cystatin ... cytometry and the median of the fluorescence of the cell population was calculated, corrected for the control value and then related to the degree of labelling of the protein used Dashes and rings ... internalization of cystatins could be a physiological pathway and thus might be important in processes such as the inhibition of virus replication and tumour growth, we addressed the question of...

Ngày tải lên: 16/03/2014, 06:20

... production of ROS and antioxidant defense mechanisms [1] It is a key component of inflammation and inflammatory disorders The processes associated with inflammatory responses are complex and often involve ... figure of control and (A)–(E) is shown in green fluorescence alone 6050 FEBS Journal 274 (2007) 6046–6054 ª 2007 The Authors Journal compilation ª 2007 FEBS O2–-scavenging effects and toxicity of ... newly synthesized and used four types of organic selenium compound: two types of selenoureas, selenourea A selenourea B, and two types of tertiary selenoamides, selenoamide A and selenoamide B...

Ngày tải lên: 23/03/2014, 07:20

... number of translations per mRNA molecule may FEBS Journal 274 (2007) 783–790 ª 2007 The Authors Journal compilation ª 2007 FEBS 785 Fast set-up of inducible protein expression M Bach et al Table Regulation ... a factor of 9.7) Thus, the background in the off-state was much lower with pEBTetD than with pEBTet However, in contrast to expression of membrane proteins, the level of expression of cytosolic ... shading’ [9] Our strategy is based on comparative analysis of lysates of cells both with and without transporter expression The expression of all other proteins in the two cell populations should...

Ngày tải lên: 23/03/2014, 09:21

... effects of hypoxia on the expression of HO-1 and HO-2 in human cell lines of Reduced expression of heme oxygenase-2 A B Fig Effects of hypoxia on expression of heme oxygenase (HO)-1 and HO-2 ... the expression levels of HO-1 and HO-2 proteins, detected by western blot analysis Opposite effects of hypoxia on expression of HO-1 and HO-2 mRNA in YN-1 cells B Fig Decreased expression of heme ... because CdCl2 induced HO-1 expression [10,43] Effects of actinomycin D on the expression of HO-1 and HO-2 mRNA To study the effects of hypoxia on the stability of HO-1 and HO-2 mRNA, YN-1 cells were...

Ngày tải lên: 19/02/2014, 06:20

... Results and Discussion MicroRNA expression profiling in mature CD8+ T lymphocyte subsets Different subsets of human CD8+ T lymphocytes can be defined based on the expression of CCR7, CD45RA and CD28 ... Journal of Translational Medicine 2011, 9:44 http://www.translational-medicine.com/content/9/1/44 Page of Figure Sorting of human CD8+ T lymphocytes and microRNA large scale expression profiling ... view of proteomic experiments demonstrating that a single miRNA can directly repress the production of hundreds of proteins in mammalian cells, through both downregulation of mRNA levels and translation...

Ngày tải lên: 18/06/2014, 19:20

... Results and Discussion MicroRNA expression profiling in mature CD8+ T lymphocyte subsets Different subsets of human CD8+ T lymphocytes can be defined based on the expression of CCR7, CD45RA and CD28 ... Journal of Translational Medicine 2011, 9:44 http://www.translational-medicine.com/content/9/1/44 Page of Figure Sorting of human CD8+ T lymphocytes and microRNA large scale expression profiling ... view of proteomic experiments demonstrating that a single miRNA can directly repress the production of hundreds of proteins in mammalian cells, through both downregulation of mRNA levels and translation...

Ngày tải lên: 20/06/2014, 03:20

... but expression of the isoform ki- and N-RAS was not excluded [5] Activated RAS was detected with an anti-(pan RAS) Ig, which does not distinguish the various isoforms of RAS In Regulation of RAS ... the links of the pathway For instance, MP1 can bind MEK and ERK1 supporting ERK1 activation [26], and kinase suppressor of RAS can bind RAF, MEK and ERK favouring activation of MEK and ERK1-2 ... [28,29] A reduction of the expression of the scaffolding proteins or an over -expression of proteins responsible for down -regulation could be an explanation for the inefficiency of RAS to activate...

Ngày tải lên: 08/03/2014, 16:20

... substrates, we showed that repair of the 3¢-OH and 5¢-phosphate strand break is accomplished within min, whereas only a small fraction of the 3¢-phosphate and 5¢-OH strand break was processed after ... several cycles of PARP-1 binding and dissociation We further speculate that this repetitive binding of PARP-1 is required only when the number of unrepaired strand breaks exceeds the amount of rate-limiting ... that processing of uracil in human cell extracts may be divided into two major steps: removal of the damaged base and incision of the generated AP site as a first step and processing of the incised...

Ngày tải lên: 23/03/2014, 13:20

... types of tumours as indicated in the figure and include carcinomas of different types (squamous and adenocarcinomas), sarcomas and T and B lymphomas (B) The mobility of endogenous VRK2A and VRK2B ... localization Substrate specificity of VRK2 isoforms and p53 phosphorylation To identify and confirm the subcellular localization of both VRK2 isoforms the cDNA of each isoform was cloned in pCEFL–HA ... Differential stabilization of p53 by VRK2 isoforms Fig Expression of two VRK2 isoforms (A) Detection of VRK2 proteins in several cell lines that were determined by immunoblotting of whole-cell extracts...

Ngày tải lên: 30/03/2014, 11:20

... Figure dasatinib on (A) invasion and (B) migration in HT144 and Sk-Mel-28 melanoma cell lines Effect of4 Effect of dasatinib on (A) invasion and (B) migration in HT144 and Sk-Mel-28 melanoma cell ... Comparison of IC50 concentrations of temozolomide when tested alone and in combination with dasatinib in HT144, Lox-IMVI, Malme-3M and Sk-Mel-28 cells Standard deviations represent average results of ... experiments Page of 11 (page number not for citation purposes) Journal of Translational Medicine 2008, 6:53 http://www.translational-medicine.com/content/6/1/53 Effect of dasatinib on apoptosis and cell...

Ngày tải lên: 18/06/2014, 15:20

... of 12 0.051; out of 20 0.017; out of 59 0.062; out of 16 0.039; out of 25 0.165; out of Also available online at http://ccr.coriell.org/Sections/Support/Global/QCgenotype.aspx?PgId=412 Page of ... as measurement of growth factor production and response, expression of cell cycle proteins, and cell morphology, hold a piece of the puzzle as to why certain wounds not heal Part of the challenge ... Debridement has become the standardof-care in patients with diabetes and a foot ulcer, pressure ulcers and venous ulcers, to remove necrotic and infected tissue and stimulate healing In this...

Ngày tải lên: 18/06/2014, 15:20

... (30-50%), (50-75%) and (> 75%) The score of each slide was the sum of intensity and positive rate scores The staining results were categorized as low expression (the score ≤ 3) and high expression (the ... 3C) Hou et al Journal of Translational Medicine 2011, 9:64 http://www.translational-medicine.com/content/9/1/64 Page of 10 Figure Analysis of the expression and localization of phosphorylated ... phospho-S6 kinase, and phospho-mTOR (mammalian target of rapamycin) [17] In the current study, we employed and evaluated the effect of SU11274 on proliferation, cell cycle and migration of RMS cells...

Ngày tải lên: 18/06/2014, 19:20

... phosphorylation and therefore inactivation of the ACC1 isoform of acetyl CoA carboxylase (ACC) [12] or suppression of lipogenic gene expression, including ACC1 and fatty acid synthase, and increase of fatty ... regulator and suppressor of malignant melanoma cell growth, further promoting evidence of expanding the testing of the role of AMPK activation for melanoma treatment Metformin is a derivative of guanidine, ... stimulating the growth of one cell line in our panel, and the antagonistic effects of the combination of metformin and vemurafenib in several cell lines, the combination of vemurafenib and metformin should...

Ngày tải lên: 18/06/2014, 19:20

... absence of 75L expression as a result of a FV3 infection in BGMK cells Therefore, the lack of detectable 75L expression may be a property of the 75L gene and not a defect in viral driven gene expression ... including the use of cryptic slice sites with the pre-mRNA, mRNA instability motifs, and RNA polymerase II termination sites [16] Another reason for poor levels of expression of viral genes may ... two stages Journal Of Virology 1982, 43:519-528 Gravell M, Granoff A: Virus and renal adenocarcinoma of Rana pipiens: IX The influence of temperature and host cell on replication of frog polyhedral...

Ngày tải lên: 20/06/2014, 01:20

... (30-50%), (50-75%) and (> 75%) The score of each slide was the sum of intensity and positive rate scores The staining results were categorized as low expression (the score ≤ 3) and high expression (the ... 3C) Hou et al Journal of Translational Medicine 2011, 9:64 http://www.translational-medicine.com/content/9/1/64 Page of 10 Figure Analysis of the expression and localization of phosphorylated ... phospho-S6 kinase, and phospho-mTOR (mammalian target of rapamycin) [17] In the current study, we employed and evaluated the effect of SU11274 on proliferation, cell cycle and migration of RMS cells...

Ngày tải lên: 20/06/2014, 03:20

... regulator of gene expression The genetic basis of gene expression has been investigated across tissues [19] and populations [20] Both lines of evidence suggest genetic variants associated with gene expression ... in the regulation of gene expression To examine the role of methylation in gene expression variation, we compared methylation levels to estimates of gene expression based on RNAsequencing (Figure ... Characterizing the genetic control of methylation and its association to the regulation of gene expression is an important area for research, critical to our understanding of how complex living systems...

Ngày tải lên: 09/08/2014, 22:23

... in near normal growth, and produced a marked decrease in skin lesions and a profound reduction in the expression of HIV mRNA and gp120 Sha et al [10] reported the results of a clinical study in ... Inhibition of TNF release can occur at a number of different points in stimulated cells These might include initial binding of the stimulating ligand, transcription of TNF mRNA, translation of mRNA ... potently inhibits the release of TNF and MCP-1 (91 and 95% 33 25 28 57 10 25 30 32 95 38 91 respectively) with a lesser effect on the release of IL-1 and IFN-γ (33 and 38% respectively) In contrast,...

Ngày tải lên: 10/08/2014, 05:20