expression purification and polyclonal antibody production

... EBNA-1 and BHRF-1 DNA targets into plasmid vector pGEM and standard curve construction A novel feature of the assay was the design of a quantification standard incorporating both EBNA-1 and BHRF-1 ... the necessity for two separate EBV standards, thus reducing costs and labour The EBNA-1 and BHRF-1 DNA targets were linked using randomised primers (Table 1) and inserted into the pGEM vector, ... between EBNA-1 and BHRF-1 DNA loads and VCA IgG antibody titres Results Performance of EBV QPCR assays: reproducibility, sensitivity, detection limit and specificity The intra-assay and inter-assay...

Ngày tải lên: 12/08/2014, 01:22

... Muhimbili University of Health and allied Sciences We wish to thank Dr Kazema and Dr Kimaro (X-ray and imaging specialists) who read all the X-rays We thank and Mr Ngowi and Mr Shogolo (Laboratory ... received pre- and post-test counseling and the HIV test was done using a dual ELISA algorithm Sera which were non-reactive on first ELISA were considered HIV antibody negative, and those reactive ... smear negative and AFB sputum If HIV Positive Clinical assessment Request CXR Give CPT Visit Treat for TB and give CPT if HIV + Visit TB likely: treat for TB and give CPT CXR suggestive and clinical...

Ngày tải lên: 15/03/2014, 03:20

... M ( MI i ,k ) and D( MI i ,k ) are pre-calculated and tabulated, and requiring time O(ia) and O (ia2), respectively We pre-calculate these values for k = 2, ,200; ia = 1, ,500 and store them ... of the algorithms and data analysis, performed the programming and drafted the manuscript MSG participated in the data collection and analysis and manuscript preparation AAM and ARR participated ... shown in [20], and reflects proximity of two distributions, in our case, the gold standard and the predicted grouping of sequences In the case of generated families we have the standard of truth...

Ngày tải lên: 12/08/2014, 17:20

... according to standard methods at SVA, which comprise fermentation of glucose, maltose, lactose, sucrose, starch, mannitol and fructose, and production of lecithinase, tryptophanase, urease and hydrolysis ... contributions EB and SSL designed the study and EB performed the practical analyses and writing the manuscript, with some assistance from SSL KEJ was responsible for the PCR and sequence methods, and discussed ... manure, soil, silage and substrate from a biogas plant were analysed The samples from the biogas plant were from both before and after pasteurisation and from digested residues Soil and manure were...

Ngày tải lên: 12/08/2014, 18:22

... magnitudes of shift in X and Y, various levels of autocorrelation of X and Y, and correlation between X and Y should be investigated The shift sizes in X and Y are set to 0, 0.5, 1, and Further, the ... indeed inappropriate, for monitoring, controlling and improving process quality Alwan and Roberts (1988), Wardell, Moskowitz and Plante (1992), Lu and Reynolds (1999), Hwarng (2004a, 2005a) etc ... higher Lu and Reynolds (1999) extensively studied the performance of the EWMA chart based both on the residuals and on the original observations of the AR(1) process with a random error Lu and Reynolds...

Ngày tải lên: 04/10/2015, 15:45

... endoscopic and radiographic Asymptomatic colonic and gastric carcinoma may present with IDA and exclusion of these conditions is of prime concern The upper endoscopic evaluation should include random ... criteria and were enrolled Their mean age was 43.3 (19-81) years 71 were patient aged under 50 and 20 were over 50 years 77 were female and 14 were male Sixty-six of women were pre-menopausal and ... cancer and small intestinal cancer [19-20] The standard procedure for investigating the source of IDA among men and postmenopausal women is to rule out gastrointestinal tract pathology and a nutritional...

Ngày tải lên: 25/10/2012, 11:18

... contamination sources settle on river and estuarine sediment, and that the sediments may protect the bacteria from rapid decay as suggested by Gerba and McLeod (1976) and Anderson et al (2005) On the ... estuarine sediments (St D and E; GM = 4.7 x 103 and 4.9 x 103 CFU/100 g dry weight, respectively) and then marine sediments (St A, B and C; GM = 7.3 x 102, 4.6 x 102 and 4.5 x 102 CFU/100 g dry ... sediment in the Takagi River and it is suggested that more reliable and sensitive preparation method for RNA viruses in sediment with high proportion of silt and clay and with presence of humic...

Ngày tải lên: 05/09/2013, 10:15

... ratio, and engine knock occurs more easily if the compression ratio is increased In order to fully understand the knock phenomenon and to find possible way to avoid knock phenomenon, Moses [16], and ... zones (burned and unburned) The first law and state of thermodynamic equations are expressed in differential form and integrated by Runge-Kutta method The equations for the pressure and temperature ... speed and 360 bTDC spark timing These figures illustrate that NO and CO concentration levels increase by increasing XDS value and compression ratio XDS is the ratio of cylinder diameter and stroke...

Ngày tải lên: 05/09/2013, 15:28

... Aerobics Research; and Dr Van S Hubbard, National Institute of Diabetes and Digestive and Kidney Diseases The Practical Guide Identification, Evaluation, and Treatment of Overweight and Obesity in ... physical activity, and behavior changes and can be used for assessment, treatment, and followup during weight loss and weight maintenance The relationship between the practitioner and these professionals ... patient’s health care and self-care Expect and allow modifications to a program based on a patient’s response and preferences Assessment and Classification of Overweight and Obesity You can calculate...

Ngày tải lên: 14/02/2014, 09:20

... The 2nd and 3d columns show the total number of cues within the datasets; the 4th and 5th columns show the percentage of negated and speculative sentences 70% of the corpus documents (randomly ... speculation and negation scopes could be concisely expressed as a 284 combination of lexical and syntactic patterns For ¨ u example, Ozg¨ r and Radev (2009) examined sample BioScope sentences and developed ... also manually compiled and used in a number of hedge scope detection systems, e.g (Kilicoglu and Bergler, 2008), (Rei and Briscoe, 2010), (Velldal et al., 2010), (Kilicoglu and Bergler, 2010), (Zhou...

Ngày tải lên: 20/02/2014, 04:20

... separates pro and candidate sentences (CR and PR in Table 1) from sentences irrelevant to either of them (NR) The classification task then classifies candidates into pros (PR) and cons (CR) Section ... Cardie, C., Riloff, E., and Patwardhan, S 2005 Identifying Sources of Opinions with Conditional Random Fields and Extraction Patterns Proceedings of HLT/EMNLP-05 Esuli, Andrea and Fabrizio Sebastiani ... players and restaurant reviews We divided data into 80% for training, 10% for development, and 10% for test for our experiments 5.1 Experiments on Dataset Identification step: Table and show pros and...

Ngày tải lên: 20/02/2014, 12:20

... drinks and confectionery high in added sugars other food and drinks high in fat and sugar, such as some takeaway and fast foods Eat breakfast • Watch the portion size of meals and snacks, and how ... implementing the England and Wales National Service Frameworks for children, young people and maternity services (the Children’s NSFs for England and Wales), and ‘Every child matters’ and similar initiatives ... by 2010 and implementing the Children’s NSFs for England and Wales, the National Healthy Schools Programme (and the Welsh Network of Healthy Schools Schemes), and ‘Every child matters’ and similar...

Ngày tải lên: 21/02/2014, 11:20

... governance, compliance, and ethics, and their collective role in preventing and mitigating excesses and scandals in the corporate sector Earlier rounds of corporate scandal gave rise to the Sarbanes-Oxley ... understandings about how a corporation operates and what its chief values are To the extent that trust, honesty, and fairness become embodied in a company’s brand promise and in the shared understanding ... rousing speech and memorandum from the chief executive officer (CEO) delivered, and an “ethics officer” appointed, then all the employees and managers will join hands in a “Kumbaya” moment, and the...

Ngày tải lên: 06/03/2014, 22:20

... than milk and milk products, meat and meat products, and fish and fishery products [4] ISO/TR 11133-1, Microbiology of food and animal feeding stuffs — Guidelines on preparation and production ... International Standard is applicable to  products intended for human consumption and the feeding of animals;  environmental samples in the area of food production and food handling WARNING ... immediate, and for certain groups of products International Standards and/ or national standards may already exist that not comply with this horizontal method It is hoped that when such standards...

Ngày tải lên: 07/03/2014, 16:20

... the one hand the fact that some of the phenomena are too rare and are not captured by their models On the other hand, in many other cases parsers make correct predictions, but the gold standard ... steps: Automatic detection of error candidates, i.e cases where two parsers deliver results different to gold-standard Substitution of the annotation of the error candidates by the annotation proposed ... transition-based parser We subsitute the gold standard by MSTParser and not MaltParser in order not to give an advantage to a parser with similar basics (both MDParser and MDParser are transition-based) During...

Ngày tải lên: 07/03/2014, 22:20

... markers and noise, removing punctuation and case information, and conducting text normalization We also built automatic rule-based and statistical annotation tools for these LUCs Features and Model ... sampling approaches for handling imbalanced data for CRF training, we investigated two approaches, random downsampling and ensemble downsampling Random downsampling randomly downsamples the majority ... presence of negation and acquiescence, yes/no equivalents, positive and negative tag questions, and other features distinguishing different types of initiating utterances and responses We also...

Ngày tải lên: 07/03/2014, 22:20

... positive and negative examples, one first builds the maximal canonical automaton (MCA) (Pernot et al., 2005) with one starting state and a separate sequence of states for each positive example and ... These pairs share the second argument and it makes it possible to apply generalization in the direction ← LCS of apparatus#1 and bar#3 is instrumentality#3 and hence the generalized pair becomes ... argument is, on the one hand, useful because none of the arguments share a semantic category with the corresponding arguments of all negative examples On the other hand, it is too restrictive...

Ngày tải lên: 08/03/2014, 01:20

... solution and centrifuged at 4000 g for 15 Half of the residue was designated the hyphae fraction (HP) and the remainder was homogenized with mortar and pestle for in liquid nitrogen and suspended ... acid and vanillic acid, as a sole source of carbon Cell growth and the production of the b-aryl ether cleavage enzyme We cultured 2BW-1 in stationary test tubes at 28 °C To observe the expression ... [pH 6.0, containing 30% acetone and mg horseradish peroxidase (44 UÆmg)1, Sigma)] and stirred for 14 h at 20 °C After the additions, mg of peroxidase was added and the reaction mixture was stirred...

Ngày tải lên: 17/03/2014, 03:20

... above.6 • Main rivers and lakes: Within the landscape rivers and lakes have two different functions On one hand they are natural barriers separating landscapes, and on the other hand they can serve ... Land cover The land cover data was provided by the Ministry of Agriculture and Forestry (MAF) and represents the land cover in 2002 The dataset is part of a forest cover and land use changes ... SPOT satellite images at 1:50’000 and 1:100’000 scales and by conducting forest and land use mapping and field verification It distinguishes 24 different types of land cover, of which ten represent...

Ngày tải lên: 17/03/2014, 11:20

... sensor’s dynamic response were extracted by FFT and DWT decomposition methods, and discrimination between ethanol and H2 S measurements was performed by PCA and DFA DWT was found to outperform FFT for ... et al / Sensors and Actuators B 104 (2005) 132–139 annealing, a transition towards a monoclinic structure took place and the WO3 particles then exhibited a mixture of tetragonal and monoclinic ... between 150 and 250 ◦ C for all further measurements 3.2 Calibration curves for low concentrations of ethanol and H2 S At first, different concentrations of ethanol (2, 5, 10, 20, 30, 40, and 50 ppm)...

Ngày tải lên: 20/03/2014, 13:04