expression purification and crystallization of neisserial outer membrane proteins

... diagnostics and epidemiologic surveys Methods Strains, plasmids, and enzymes Expression and purification of LSV coat protein Figure Expression and purification of LSV coat protein A, Expression and purification ... 23th candidate proteins had scores from 82 to 178, those were all coat proteins of various isolates of LSV, and protein scores greater than 67 were significant (p < 0.05) 15 of 28 mass values of ... Alignment of the amino acid sequence of coat protein of different LSV isolates and other carlavirus Figure Alignment of the amino acid sequence of coat protein of different LSV isolates and other...

Ngày tải lên: 12/08/2014, 04:21

... BPH and PC) by immunohistochemistry to evaluate the relationship between the co -expression of prostate- associated Page of antigens (PSMA and PSA) and the degree of vascularization (intensity of ... Page of secretory epithelial cells of prostate gland, both PSMA and PSA transcriptions are androgen-dependent [39,40] The emergence of androgen-insensitive tumor cells arising as a consequence of ... followup of PC [23] Unlike serum PSA, there are drawbacks to use tissue PSA, like for example, the loss of expression of tissue PSA associated with advanced disease and the development of androgen-independent...

Ngày tải lên: 10/08/2014, 10:20

... ordering process and the cost of synthesis is relatively cheap An example of appropriate design of primers to add attB1 and B2 sites to the 5′ and 3′ ends respectively of a gene of interest are ... N-glycosidic bonds between uracil and deoxyribose leading to a loss of the base The loss of bases leads to disruption of base pairing and therefore exposure of single strands that can be annealed to ... ethidium bromide and UV irradiation for detection of DNA in gels, thereby preventing DNA damage of the long molecules and enhancing isolation of full-length clones The range of cloning kits and vectors...

Ngày tải lên: 25/10/2013, 22:20

... acids), and two membraneanchor proteins (CybL, 15 kDa, 140 amino acids, and CybS, 11 kDa, 103 amino acids) with a total of six transmembrane helices Here we provide a detailed report of the preparation, ... sequencing and crystallization of mitochondrial complex II from porcine heart Characterization of respiratory complex II be the case that some indispensable cofactors (i.e phospholipids) for crystallization ... crystallization preparation and the crystal of complex II Lane 1, crystals of complex II Lane 2, molecular weight markers Lane 3, crystallization preparation of complex II The four subunits of complex II are...

Ngày tải lên: 19/02/2014, 02:20

... indicate the ratio of dissociation of water and dissolution of TiO2 and this ratio is the key factor in governing the porosity of the ATO films, as we discuss later The protons in the form of H3O+ move ... dissolution rate of TiO2 is fast, resulting in a reduction of thickness (d) of the oxide layer and therefore an increase of the field strength The dissociation rate of water will then be increased and the ... along and perpendicular to the wall It is Fig TEM images of (a) a cluster of ATO nanotubes and (b) a single nanotube, showing an O-ring pattern on the outer surface of the nanotubes (c) and (d)...

Ngày tải lên: 19/03/2014, 16:48

... determination Crystallization and preliminary crystallographic analysis of the bacterially expressed enzyme are reported Materials and methods Organisms and cell fractionation Bloodstream forms of T brucei ... mixture (50 lL total volume) contained lg of genomic DNA from T brucei stock 427, 100 pmol of each primer, 250 lM of each of the four deoxynucleotides, and U of rTaq DNA polymerase with the corresponding ... concentration of mM, when the culture reached an absorbance (A) at 600 nm of % 0.5, to induce expression of the protein, and growth was continued overnight at 30 C Cells were collected by centrifugation and...

Ngày tải lên: 23/03/2014, 21:20

... complex and assembled into a particulate structure in the absence of genomic RNA Figure Assembly of VLPs by expression of RVFV N and Gc proteins Assembly of VLPs by expression of RVFV N and Gc proteins ... purification of proteins and VLPs, and EM studies CC carried out cell surface expression and cell to cell fusion studies PR contributed in the coordination and design of the study and helped in ... Fusogenic activity of RVFV Gn and Gc proteins Fusogenic activity of RVFV Gn and Gc proteins A) Insect cells were infected with a baculovirus expressing RVFV N, Gn and Gc for 24 hours and a monoclonal...

Ngày tải lên: 20/06/2014, 01:20

... the two-step chromatography of AEX and HIC was sufficient for the purification of FIII-1, FIII-2, and FII, since their SEC and SDS-PAGE profiles represented pure proteins Fraction FI actually ... thankful to Professor Les Copeland (Faculty of Agriculture, Food and Natural Resources - The University of Sydney, New South Wales, Australia) and Professor Kaeko Kamei (Department of Biomolecular ... alignment of fragments obtained from MS/MS analyses of FIII-1 and FIII-2 from P excavatus with lumbrokinase and its precursor from L rubellus (sp:P83298 and U25647) and E fetida (gpu:EU167737 and AY438624),...

Ngày tải lên: 20/06/2014, 23:20

... citatsomreht a ot detcennoc )nedewS ,aicamrahP( tinu IIrohpitluM eht ni demrofrep saw sisylana citerohportcele ehT la te gnisucof cirtceleosi lacitylanA snoitacifidom thgils htiw ]42[ nibwoT fo dohtem ... ,BKL( rettolb yrd-imes tolBavoN a gnisu demrofrep saw sisylana tolbonummI la te gnittolbonummI ]31[ ilmmeaL yb debircsed snoitidnoc eht ot gnidrocca demrofrep saw siserohportcele dna ,selpmas ,sleg ... noitaraperP )ASU ,daRoiB( lleC balS-lauD II naetorP -iniM eht ni demrofrep saw sisylana citerohportcelE EGAP-SDS ]3[ dohtem drofdarB eht yb detamitse saw sPLG eht fo tnetnoc nietorp ehT fo sPLG...

Ngày tải lên: 07/08/2014, 20:23

... 2) identify the structural difference of duplicated genes, and 3) reveal the expression and evolution of fiber quality differences between upland and sea-island cotton To complete this study, we ... initiation (0 and DPA) and DPA and at and DPA was reversed At 20 DPA, Sus1 expression was significantly higher in TM-1, possibly as a result of earlier termination of fiber elongation and earlier ... species Gene Numbers of exon Length of ORF(bp)/numbers of derived amino acids 14-3-3L 762/253 CAP A1, Ath and Atb: 10; D5, Dth and Dtb: A1, Ath and Atb: 1416/471; D5, Dth and Dtb: 1338/445 CEL...

Ngày tải lên: 11/08/2014, 11:21

... Journal 2010, 7:241 http://www.virologyj.com/content/7/1/241 Page of Figure Expression and purification of the tgK protein M represented standard protein molecular weight markers The arrow marked the ... to detect the reactivity and specificity of the tgK The purified recombinant proteins were separated on 12% SDS-PAGE and transferred onto polyvinylidene fluoride (PVDF) membrane at 120 V for 1.5 ... http://www.virologyj.com/content/7/1/241 Page of Figure Purification of the rabbit anti-tgK polyclonal antibody and Western blot assay M represented standard protein molecular weight markers; M1...

Ngày tải lên: 12/08/2014, 01:21

... intron 5, and microsatellite SJ158 and a novel microsatellite marker that includes a tandem repeat of (TC)n were identified in intron Primers SJ16 and SJ15, designed from the primer sequences of pig ... bijk is the regression coefficient of the slaughter weight and eijk is the random residual RESULTS 3.1 Expression pattern of porcine CA3 gene During the three stages of skeletal muscle development ... Differential expression analysis of the porcine CA3 gene in different pig breeds Real-time PCR analysis of the CA3 gene in the skeletal muscle of Yorkshire and Meishan pigs at month-2 Relative levels of...

Ngày tải lên: 14/08/2014, 13:22

... Structures and molecular weights of soyasapogenol B, C and B1 98 Fig 28 LC-MS Chromatogram of standard and purified sample of soyasapogenol B Panels (A and C) are authentic standard and samples of ... 79 6.2.1.5 HPLC Analysis of Soyasaponins and Soy isoflavones 79 6.2.1.6 LC/MS Analysis of Total soyasaponins and Soy isoflavones 79 6.2.2 Comparison of Soyasaponin Concentration Methods ... recovery of soyasapogenols A and B without producing artifacts (Ireland and Dziedzic 1986; Rupasinghe, Jackson et al 2003) Moreover, Ireland and Dziedzic (Ireland and Dziedzic 1986) showed that...

Ngày tải lên: 11/09/2015, 10:06

... microglia altered the expression of proinflammatory cytokines such as TNF-α and IL-1β and inflammatory mediators including iNOS and NO and the migratory and phagocytic ability of activated microglia, ... studies 1.11 Global gene expression profiling of microglia Global gene expression profiling of amoeboid and ramified microglia were performed by isolating the amoeboid and ramified microglia from ... and transform into a rounded activated phenotype Adapted and modified from (Streit and Xue, 2009; Gomez-Gonzalez and Escobar, 2010) 1.4 Functions of microglia 1.4.1 Chemotaxis and migration of...

Ngày tải lên: 12/09/2015, 11:24

... hexakisphosphate) is the primary storage form of phosphorus in plants They occupy about 1-3% of the seeds of grains and legumes, and 60-80% of the total amount of plant phosphorus (Nelson, 1967) Phosphorus ... PuriWcation and structural characterization Protein Expression and Purification 46, pp.221–232 Bradford, M.M 1976 A rapid and sensitive method for quantitation of microgram quantities of protein ... determination of inorganic orthophosphate and it’s application to the assay of inorganic pyrophosphate Anal Biochem 113:313–317 Kim, Y O., Kim, H K., Bae, K S., Yu, J H and Oh, T K 1998 Purification and...

Ngày tải lên: 06/10/2015, 13:05

... images of membrane with 1:20 ratio of VBC to AN (a) the surface cross-linked with EDA; (b) & (c) cross section of 1: 20 and 1: 40 ratio of VBC to AN respectively (cross-linked with EDA) and (d) ... their work, PEM is consists of an outer layer of sulfonated polyphenyl sulfone (S-PPSU) and the inner layer blend of octa-sulfonated octaphenyl POSS and S-PPSU This membrane has excellent physical ... 20 Membrane Anion exchange membrane Fuel Several studies have reported the performance of the membrane in various conditions like different types of fuel, concentration of fuels, electrolyte, membrane...

Ngày tải lên: 16/10/2015, 11:58

... Letters denote the five residues of one of the three sides of sbwAFP or six residues of one of two sides of TmAFP The strands that make up the three, parallel b-sheets of the protein are designated ... structure and PDB code shown below each panel (A) Overlap of isoform 337 with the structure of the longer isoform CfAFP-501 using the main chain of residues Thr23fiAsn90 in isoform 337 and residues ... orientations of the N- and C-termini of the proteins are inverted with respect to one another (B) Stereo view of a cross-section of an overlapped coil of the sbwAFP (residues Gly34 to Thr49, red) and...

Ngày tải lên: 19/02/2014, 16:20

... effect of the proteins, all the H-T solutions contained the indicated concentrations of proteins RESULTS Identification, isolation and cloning of tigrin and ablomin During the isolation process of ... specificity and mechanism of action Isolation and characterization of homologous proteins, triflin and latisemin In subsequent experiments, we isolated two other homologous proteins and cloned ... obtained mg of triflin and mg of latisemin from 500 mg of crude venom, respectively The predicted amino-acid sequences of triflin and latisemin are homologous to that of ablomin (83.7 and 61.5%,...

Ngày tải lên: 18/03/2014, 01:20

... [19] and Hedge & Das [20] were studying the proteins p36 and p38, respectively The proteins may be identical: both proteins are phosphorylated in response to the presence of Ras protein and guanine ... twocomponent proteins Two-component systems are present in both Gram positive and Gram negative bacteria In addition to housekeeping functions, they also control expression of toxins and other proteins ... of phosphohistidine in proteins and purification of protein-histidine kinases Methods Enzymol 200, 388–414 Kamps, M.P (1991) Determination of phosphoamino acid composition by acid hydrolysis of...

Ngày tải lên: 24/03/2014, 03:21

... neuron and postsynaptic muscle fiber contribute to the synaptic expression of PRiMA-linked G4 AChE and illustrate its temporal and spatial expression at NMJs Results Regulation of G4 AChE and PRiMA ... recognized both PRiMA and PRiMADC-term with protein bands of approximately 20 and 16 kDa, respectively: these protein bands corresponded to the predicted size of the recombinant proteins (Fig 1A) ... than that of AChE and AChR, extending into a peri-junctional zone where neither 3034 Fig Expression of PRiMA and G4 AChE in different muscles (A) Samples of extracts from adult rat soleus and tibialis...

Ngày tải lên: 18/02/2014, 08:20