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COLLOIDAL BEHAVIOR OF HIGHLY BRANCHED POSS PARTICLES AND DEVELOPMENT OF ANION EXCHANGE MEMBRANE AKLIMA AFZAL NATIONAL UNIVERSITY OF SINGAPORE 2010 COLLOIDAL BEHAVIOR OF HIGHLY BRANCHED POSS PARTICLES AND DEVELOPMENT OF ANION EXCHANGE MEMBRANE AKLIMA AFZAL (B. Sc. in ChE, Bangladesh University of Engineering and Technology, Bangladesh) A THESIS SUBMITTED FOR THE DEGREE OF MASTERS OF ENGINEERING DEPARTMENT OF CHEMICAL & BIOMOLECULAR ENGINEERING NATIONAL UNIVERSITY OF SINGAPORE 2010 National University of Singapore ACKNOWLEDGEMENTS I would like to express my heartfelt gratitude to my supervisor, Associate Professor Hong Liang and co supervisor, Dr. Liu Zhao Lin for their sincere cooperation at every stage of my research. A/P Hong Liang’s valuable advice and patient guidance always guided me to conduct my research smoothly. I am very much thankful to Dr. Zhang Xinhui and Dr. Tay Siokwei for their inspiration, advice and suggestion in carrying out the synthesis part. I owe my deep gratitude to National University of Singapore for providing research scholarship and Chemical and Biomolecular Engineering Department for the facilities provided to carry out his project through in its entirety. Thanks are also given to the department stuff members for providing various types of help during this work. I want to take a privilege to convey my thanks and gratitude to my colleagues as well as friends Guo Bing, Liu Lei, Chen Xinwei and Sun Ming for their support and encouragement during the study period. It is a pleasure to thank my friends namely, Rajib and Iftekhar for their extended help and support whenever I needed. I am greatly indebted to Abu Zayed for his continuous assistance and inspiration to perform my work. Sincere thanks also go to Md. Mazharul for his selfless guidance and immense effort during reviewing my thesis. Finally, I would like to thank my family for their selfless love and full support at every stage of my life. Without their encouragement and spiritual support, it would have been difficult for me to stay in abroad and complete this dissertation. National University of Singapore i TABLE OF CONTENTS ACKNOWLEDGEMENTS .................................................................................. i TABLE OF CONTENTS ..................................................................................... ii SUMMARY ......................................................................................................... vii LIST OF TABLES ............................................................................................. viii LIST OF FIGURES ............................................................................................. ix NOMENCLATURE ............................................................................................. xi CHAPTER ONE INTRODUCTION ..................................................................1 1.1. Preamble .......................................................................................................1 1.2. Objective of the Study ..................................................................................3 1.3 Scope of the Study .........................................................................................4 1.4 Organization of this Thesis ............................................................................5 CHAPTER TWO LITERATUR REVIEW .........................................................7 2.1 Introduction ....................................................................................................7 2.2 Polyhedral Oligomeric Silsesquioxane (POSS) .............................................8 2.2.1 Types of Silsesquioxane .........................................................................8 National University of Singapore ii Table of Contents 2.2.2 Properties of POSS ...............................................................................10 2.2.3 Researches’ on POSS as an Additive....................................................12 2.2.4 Applications of POSS as Non-additive .................................................15 2.3 Dendrimer ....................................................................................................17 2.4 Fuel cells (FC)..............................................................................................19 2.4.1 Fuel cell theory .....................................................................................20 2.4.2 Classification of fuel cells .....................................................................21 2.4.3 Solid Alkaline Fuel Cell........................................................................22 2.4.4 Anion Exchange Membrane (AEM) .....................................................24 2.4.5 Studies on AEM ....................................................................................25 2.4.6 Fuel Electrolyte Assembly for AEMFC ...............................................27 2.5 Summary ......................................................................................................29 CHAPTER THREE MATERIALS AND METHODS.....................................31 3.1 Introduction ..................................................................................................31 3.2 Materials ......................................................................................................31 3.3 Experimental Framework.............................................................................32 3.3.1 Synthesis for colloidal study .................................................................32 3.3.1.1 Synthesis of G0-HEMA with two branches.................................. 33 3.3.1.2 Synthesis of G0-HEMA with Quaternary Amine ......................... 35 National University of Singapore iii Table of Contents 3.3.1.3 Synthesis of G0-HEMA with three branches................................ 36 3.3.2 Synthesis of Anion Exchange Membrane .............................................37 3.3.2.1 Synthesis of the backbone ............................................................. 38 3.3.2.2 Preparation of the Charge Carrier Group ...................................... 39 3.3.2.3 Grafting the monomer on to the backbone via ATRP .................. 39 3.3.2.4 Membrane casting ......................................................................... 40 3.4 Characterization and Analytical Tools.........................................................41 3.4.1 Fourier Transform Infrared Spectroscopy (FTIR) ................................41 3.4.2 Transmission Electronic Microscopy (TEM) .......................................41 3.4.3 Thermogravimetric Analysis (TGA).....................................................42 3.4.4 Scanning Electric Microscope (SEM) ..................................................42 3.4.5 Dynamic Light Scattering (DLSC) .......................................................42 3.4.6 Auto Lab ...............................................................................................43 3.4.7 Particle Size Analyzer (Zeta Sizer) .......................................................43 3.4.8 Gas Chromatography (GC) ...................................................................43 3.4.9 UV Cross-Linker ...................................................................................44 3.5 Summary ......................................................................................................44 National University of Singapore iv Table of Contents CHAPTER FOUR RESULTS AND DISCUSSIONS .......................................45 4.1 Introduction ..................................................................................................45 4.2 Characterization of POSS derivatives ..........................................................46 4.2.1 Characterization of G0-HEMA with two branches ..............................46 4.2.2 Characterization of G0-HEMA with Quaternary Amine ......................49 4.2.3 Characterization of G0-HEMA with three branches ............................50 4.3 Study of the Colloidal Behavior of POSS....................................................50 4.3.1 Dynamic Light Scattering .....................................................................51 4.3.2 Zeta potential ........................................................................................53 4.4 Kinetics study...............................................................................................56 4.4.1 Kinetic study at different hydrophobic solvents ...................................57 4.4.2 Kinetic study at different Relative Humidity ........................................59 4.5 Characterization of the membrane ...............................................................61 4.5.1 FTIR Spectrum of the Membrane .........................................................61 4.5.2 Energy Dispersion X-ray Spectroscopy ................................................63 4.5.3 Thermal Analysis ..................................................................................64 4.5.4 Surface morphology ..............................................................................65 4.6 Performance of the membrane .....................................................................66 4.6.1 Ethylene Diamine (EDA) as a cross-linker ...........................................67 4.6.1.1 Conductivity.................................................................................. 67 National University of Singapore v Table of Contents 4.6.1.2 Water Uptake ................................................................................ 69 4.6.1.3 Methanol Crossover ...................................................................... 70 4.6.2 Amine-POSS as a cross-linker ..............................................................70 4.6.2.1 Conductivity.................................................................................. 71 4.6.2.2 Methanol Crossover ...................................................................... 71 4.6.3 Comparison between Membranes using EDA and Amine-POSS as cross-linker .....................................................................................................72 4.6.3.1 Comparison of Surface Morphology ............................................ 72 4.6.3.2 Comparison of Ion Conductivity .................................................. 73 4.7 Summary ......................................................................................................74 CHAPTER FIVE CONCLUSIONS AND RECOMMENDATIONS..............75 5.1 Research contributions .................................................................................75 5.1.1 Findings from colloidal study ...............................................................75 5.1.2 Findings from Anion Exchange Membrane ..........................................77 5.2 Recommendations ........................................................................................78 REFERENCES .....................................................................................................80 National University of Singapore vi SUMMARY This research focuses on the study of the colloidal behaviors of highly branched polyhedral oligomeric silsesquioxane (POSS) particles synthesized by performing dendrimerization from the pendant functional group (i.e. propylmethacryl) at the eight corners of cubic POSS molecule. As the first targeted POSS dendrimer, 2hydroxyethyl methacrylate (HEMA) was employed to crown the dendrimerization, it bears therefore bulky hydroxyl end groups. Colloidal properties of this nano-particle have been studied with three structures of POSS. Results show that G0-HEMA particle with two branches undergoes both the protonation and deprotonation with the increase in pH. Besides this, the particle may show finite affinity with anion of boric acid because of the terminal hydroxyl groups. However, hydrophobic POSS core was found to retard the responding capability of the pendant hydroxyl groups to the change of pH and concentration of boric acid. The hydrophilicity of the G0-HEMA was enhanced via increasing branches of HEMA and converting the tertiary amine groups in the inner grafting layer of this particle to quaternary ions. These two measures promote flexibility of the tele-HEMA branches as well as affinity with boric acid molecules. In addition to the investigation into aqueous colloidal dispersions of POSS-tele-HEMA by zeta potential, dynamic light scattering (DLS) and transmission electronic microscopy (TEM) means, thin films fabricated by spincoat the colloidal dispersions on a flat substrate was studied with the aim to understand the kinetics of conversion between hydrophobic and hydrophilic surfaces. Another focus of this research is to develop grafting anion exchange membrane by means of living radical polymerization. This polymer is employed to form a hydroxide (OH-) exchange membrane for its application in solid alkaline fuel cell (SAFC). Recently, anionic exchange membrane in SAFC has received much attention because it relies on a much cheap electrode catalyst than platinum which is the solely anodic catalyst in the proton exchange membrane fuel cell (PEMFC). Hydroxyl conductivity and mechanical test have been tested to find out the feasibility in the alkaline fuel cell application. A series of features of the obtained anion exchange membrane (AEM) has been assessed. In the next step, POSSbased cross-linker will be incorporated into this membrane system to revamp the performance of it in SAFC. Experimental results yield that the di-block membrane with ethylene diamine cross-linker has a good conductivity at a high temperature and the membrane is super resistant to methanol. However membrane with amine-POSS cross-linker does not show a good conductivity and surface becomes non-homogenous. In short, this research has successfully developed highly branched POSS and provides some useful insights into its properties particularly related to oscillating behaviors, adsorption of borate ion, kinetics, and cross-linking performances. National University of Singapore vii LIST OF TABLES Table 2.1 Classification of Fuel Cell .................................................................... 22 Table 2.2 Different types of Anion Exchange Membrane electrolyte assembly .. 28 Table 4.1 Effect of relative humidity on the surface structure ............................. 60 Table 4.2 Grafting of monomer on different backbone ........................................ 63 Table 4.3 Water uptake of different membranes ................................................. 69 National University of Singapore viii LIST OF FIGURES Figure 2.1 Structures of Silsesquioxanes (Li et al., 2001) ...................................... 9 Figure 2.2 Schematic diagram of POSS (3-4nm) ................................................. 11 Figure 2.3 Dendrimeric structure .......................................................................... 18 Figure 2.4 Fuel cell diagram ................................................................................. 21 Figure 2.5 Diagram of Solid alkaline fuel cell ...................................................... 23 Figure 3.1 The scheme to prepare Amine-POSS (G0) ......................................... 34 Figure 3.2 The scheme to synthesize G0-HEMA with two branches ................... 35 Figure 3.3 Scheme for G0-HEMA with Quaternary Amine ................................. 36 Figure 3.4 Scheme for synthesizing G0-HEMA with three branches .................. 37 Figure 3.5 Structure of backbone .......................................................................... 38 Figure 3.6 Synthesis scheme of the cationic monomer......................................... 39 Figure 3.7 Growing Quaternary Amine chain on Backbone ................................ 40 Figure 4.1 FTIR spectra of (a) POSS, (b) POSS-amine (G0) and (c) G0HEMAwith two branches. ............................................................................ 46 Figure 4.2 1H-NMR of G0-HEMA with two branches ......................................... 47 Figure 4.3 Transmission electron micrograph of (a) POSS and (b) G0-HEMA with two branches ......................................................................................... 48 Figure 4.4 IR spectrum of G0-HEMA with Quaternary Amine ........................... 49 Figure 4.5 TEM image of G0-HEMA with Quaternary Amine............................ 50 Figure 4.6 Size distribution of G0-HEMA with two branches at different pH..... 51 Figure 4.7 Size distribution of G0-HEMA with quaternary amine at different pH ....................................................................................................................... 52 National University of Singapore ix List of Figures Figure 4.8 Size distribution of G0-HEMA with three branches at different pH... 53 Figure 4.9 Effect of pH on G0-HEMA with two branches ................................... 54 Figure 4.10 Effect of pH and Boric Acid on G0-HEMA with Quaternary Amine on zeta potential ............................................................................................ 55 Figure 4.11 Effect of pH and Boric acid on G0-HEMA with three branches on zeta potential ................................................................................................. 55 Figure 4.12 Surface morphology of the coated layer ............................................ 56 Figure 4.13 Coated glass at different hydrophobic medium. ................................ 57 Figure 4.14 Study the effect of atmosphere on G0-HEMA coated layer.............. 58 Figure 4.15 Effect of different atmospheric condition on the surface orientation 59 Figure 4.16 FTIR spectra of backbone (a), monomer (b) and monomer grafted backbone (c) .................................................................................................. 62 Figure 4.17 Thermo gravimetric analysis of the backbone (VBC: AN = 1: 79) and monomer grafted backbone........................................................................... 64 Figure 4.18 FESEM images of membrane with 1:20 ratio of VBC to AN (a) the surface cross-linked with EDA; (b) & (c) cross section of 1: 20 and 1: 40 ratio of VBC to AN respectively (cross-linked with EDA) and (d) cross section at higher magnification. .................................................................... 66 Figure 4.19 Conductivity of the EDA cross-linked membrane vs. temperature: (a) 1: 20; (b) 1: 40; (c) 1: 79 of VBC to AN. ..................................................... 67 Figure 4.20 The Arrhenius plot of hydroxyl ion conduction for VBC to AN ratio of (a) 1: 20; (b) 1: 40; (c) 1: 79. .................................................................... 68 Figure 4.21 Conductivity of Amine-POSS cross-linked membrane vs. temperature for VBC to AN ratio of (a) 1: 20; (b) 1: 40 and (c) 1: 79. ........ 71 Figure 4.22 FESEM pictures of the membrane surface (1: 20) cross-linked with (a) EDA and (b) amine-POSS and cross section cross-linked with (c) EDA and (d) amine-POSS. .................................................................................... 73 National University of Singapore x NOMENCLATURE POSS Polyhedral Oligomeric Silsesquioxane HEMA 2-hydroxyethyl methacrylate HEM Hydroxide (OH-) Exchange Membrane SAFC Solid Alkaline Fuel Cell PEMFC Proton Exchange Membrane Fuel Cell DMFC Direct Methanol Fuel Cell SOFC Solid Oxide Fuel Cell AFC Alkaline Fuel Cell MCFC Molten Carbonate Fuel Cell MEA Membrane Electrode Assembly EDA Ethylene diamine GMA Glycidyl Methacrylate AN Acrylonitrile VBC Vinyl Benzyl Chloride DMF N, N-dimethylformamide DMPA N-(2, 3-dimercaptopropyl)-phthalamidic acid ATRP Atom Transfer Radical Polymerization Bpy 2, 2-Bipyridine FTIR Fourier Transform Infrared Spectroscopy TTAB Tetradecyl trimethylammonium bromine National University of Singapore xi Nomenclature ANS 1-anilinonaphtalene-8-sulfonate TEM Transmission Electronic Microscopy TGA Thermo-gravimetric Analysis SEM Scanning Electric Microscope DLSC Dynamic Light Scattering GC Gas chromatography National University of Singapore xii CHAPTER ONE INTRODUCTION 1.1. Preamble Polyhedral oligomeric silsesquioxanes (POSS) has attracted a wide attention from both academics and industries due to its hybrid structure of organic and inorganic materials (Wahab et al., 2008). This criterion makes POSS molecule particularly useful to a wide range of applications. POSS can be effectively incorporated into the polymer (Zhiyong et al., 2008) via different approaches like blending, grafting and copolymerization. POSS containing polymers and their derivatives can improve heat, oxidation resistance, and several mechanical properties (Zhiyong et al., 2008) and have been broadly studied due to its various applications (Xu et al., 2007) such as liquid crystals, nanocomposites, coatings and photo resists in lithographic technologies. A number of recent studies have mainly been focused to explore the properties of POSS. For example, Zucchi et al. (2009) have found that mono-functional POSS when cross-linked with polymer decreases the surface energy which could be a way to obtain hydrophobic coating without using fluorinated monomers. SanchezSoto et al. (2009) have reported that poly carbonate POSS nanocomposites enhance the tensile stress and storage module without changing the thermal behavior. Incorporating POSS polymer with different functional groups enhances mechanical properties (e.g., Lin et al., 2009; Ying-Ling et al., 2008), reduces the National University of Singapore 1 Chapter One: Introduction value of dielectric constant to ultra low value, and results a high transparency (Ying-Ling et al., 2008). POSS contents in the nano-composite are limited due to the incompatibility between POSS and polymer. Well defined block polymers are synthesized from POSS by adopting Controlled living polymerization (Xu et al., 2007). As a polymer modifier, control in phase separation is required due to thermodynamic incompatibility between POSS-rich and polymer-rich domain. This is because thermal and mechanical characteristics of nanocomposite system are significantly influenced by cross-linking density and dispersion state (e.g., Yen-Zen et al., 2007). Many studies have been focused to improve the thermo mechanical properties of the polymer by incorporating POSS molecule as an additive. Literature has showed that POSS derivatives influence both thermal and rheological properties of different polymeric materials such as polycarbonate (Sánchez-Soto et al., 2009), polypropylene (Lin et al., 2009; Zhiyong et al., 2008), polyimide (Seckin and Koytepe, 2008; Ying-Ling et al., 2008), poly amic acid (Wahab et al., 2008), polystyrene (Lei et al., 2007), epoxy (dell’Erba and Williams, 2008), and poly amide (Yen-Zen et al., 2007). While substantial work has been conducted on the property of different polymer modified by POSS, very few studies (e.g., Pyun et al., 2001) have been focused on the structure and colloidal behaviors of POSS National University of Singapore 2 Chapter One: Introduction molecules. Moreover the use of POSS as cross-linkers in anion exchange membrane has also not been found in the literature. 1.2. Objective of the Study As shown in the previous section most of the studies related to POSS were mainly focused on the thermo-mechanical behaviors of POSS. Despite of being a nano structured hybrid material; meager studies have been conducted on colloidal behaviors of highly branched POSS structures and its application as a cross-linker on di-block or anion exchange membrane. The objective of this study is to investigate colloidal behaviors of highly branched POSS structures in aqueous medium and to evaluate performances of anion exchange membrane while using ethylene diamine and amine-POSS as cross-linkers. In order to achieve this, the following research steps have been conducted: 1) Study the colloidal behavior of branched POSS synthesized by adopting the dendritic approach. Characterization of these particles has been done by using different analytic tools such as fourier transform infrared spectroscopy (FTIR) and nuclear magnetic resonance (NMR). And colloidal behaviors on different medium have been studied by using some basic analytical tools such as size distribution and zeta potential. 2) Preparation and performance evaluation of hybrid membranes that consist of both hydrophobic and hydrophilic part. While the hydrophobic backbone has been synthesized by polymerized acryl nitrile (AN), vinyl benzyl chloride (VBC), and glycidyl methacrylete (GMA), the hydrophilic National University of Singapore 3 Chapter One: Introduction quaternary amine has been grafted onto hydrophobic backbone by adopting the atom transfer radical polymerization (ATRP) approach. The performance of those membranes is studied by measuring methanol crossover and ionic conductivity. 3) Evaluate the effect of amine-POSS as a cross-linker in hybrid membranes. Generally, amine group is used to open the epoxy group (if any) present on the polymeric backbone. In this study, amine-POSS is used for this purpose and performances of amine-POSS have been compared with other type of cross-linker like ethylene diamine (EDA). 1.3 Scope of the Study Colloidal behaviors of POSS have been studied for highly branched structure of POSS. Hence three structures of POSS have been synthesized for this purpose. Effects of pH on the size and surface charge have been studied at room temperature. To observe the surface behavior of 2-hydroxyethyl methacrylate at zero generation of POSS (G0-HEMA), kinetic study on different hydrophobic medium like toluene, cyclohexane, and ethyl acetate; and on a wide range (10% 85%) of humid conditions has been conducted. Anion exchange membrane (AEM) has been prepared by grafting cationic amine group on polymeric backbone. Amine group was attached to the polymer through ATRP reaction. EDA and amine-POSS have been used as cross-linkers to open up the epoxy group. National University of Singapore 4 Chapter One: Introduction 1.4 Organization of this Thesis This thesis is organized into five chapters which explicitly explain the steps taken to achieve the objectives of this study. The structure of this thesis is briefly discussed below: Chapter 1 introduces the research background of POSS, identifies research gaps, states the objective and finally provides the structure of this thesis. Chapter 2 provides a brief description of POSS, dendrimeric structures, and fuel cell along with a critical literature review on POSS and Anion Exchange Membrane. Starting with the properties of POSS, it figures out the major field of studies related to POSS and identifies research gaps related to POSS behaviors. For anion exchange membrane, it states the benefit of using this type of membrane over proton exchange membrane (PEM). Studies related to AEM have been critically reviewed and its application in different electrolyte membrane has been discussed. Chapter 3 focuses on the experimental framework needed to achieve the aim of the study. It includes the development of POSS particles and anion exchange membrane as well as the description of the analytical tools used in this study to characterize and analyze these particles and membrane. National University of Singapore 5 Chapter One: Introduction Chapter 4 presents the results obtained from the colloidal and membrane study. It illustrates the colloidal behavior of particles and evaluates the performance of anion exchange membrane. It also presents a comparative study between membranes using two types of cross-linker. Finally, Chapter 5 summarizes the conclusions derived from this research. Some recommendations for future research are included. National University of Singapore 6 CHAPTER TWO LITERATUR REVIEW 2.1 Introduction Polyhedral Oligomeric Silsesquioxane (POSS) is relatively a new compound in the field of research. POSS has some unique characteristics like hybrid structure, dual property of organic and inorganic performance, cage like shape. Those unique characteristics of POSS make the researchers more interested in studying and understanding the behavior of POSS. This chapter presents a critical review on studies related to POSS and its use in different purposes. Starting with a basic understanding of POSS, this chapter describes the impressive features of POSS, incorporation methods into other polymers and potential features achieved while using as additives and as well as in other areas such as medical application, modification of electrolyte etc. This chapter also describes some literature on the modification of anion exchange membrane (AEM). Literature reviews on anion exchange membrane has suggested a means to modify AEM by incorporating different POSS functionalities. National University of Singapore 7 Chapter Two: Literature Review 2.2 Polyhedral Oligomeric Silsesquioxane (POSS) Polyhedral Oligomeric Silsesquioxane’s are nano sized core cage like structures constitute by an inorganic silica Rn(SiO1.5)n where n is the number of silicon atoms in cage (n= 8,10,12). The inner diameter of POSS is approximately about 1.5 nm. The core structure can be either reactive or non-reactive whereas the organic part contains reactive functionalities. The unique feature of POSS is that it has a hybrid chemical composition: (RSiO1.5), which is an intermediate between silica (SiO2) and silicone (R2SiO). Here, the functional group R can be hydrogen or any alkyl, alkylene, aryl, arylene groups or organo-functional derivatives of them. 2.2.1 Types of Silsesquioxane Polyhedral Oligomeric Silsesquioxane is one of the most popular branched of silsesquioxane groups. Silsesquioxane’s are large branch of organic-inorganic silicon containing compounds with the empirical formula R(SiO1.5). There are mainly three - types of silsesquioxane: random structures, ladder-like structures and cage like structures (Hany et al., 2005; Lei et al., 2007) as illustrated in figure 2.1. Last group also covers the partially cage like structures. National University of Singapore 8 Chapter Two: Literature Review Figure 2.1 Structures of Silsesquioxanes (Li et al., 2001) First oligomeric organo-silsesquioxane, (CH3SiO1.5)n , were isolated by Scott in 1946 through thermal analysis of polymeric products. Even after half a century, the interest in this field is still increasing due to the dual property of organic and inorganic behavior. The polymers from ladder like silsesquioxane have outstanding thermal stability and resistance from oxidative environment even at higher temperature. At the same time ladder-like silsesquioxane polymers have good insulating properties and gas permeability’s. The main application areas of National University of Singapore 9 Chapter Two: Literature Review these ladder-like oligomeric structures are in electronic and optical devices as photo resist coating, as protective coating film in semiconductors, in gas separation membranes and as a binder for ceramics and carcinostatic drugs (Li et al., 2001). Cage structured silsesquioxane (see figure 2.1, structures from c to f) is attracting much attention from the last 15 years. This cage structured polyhedral oligomeric silsesquioxane is designated as POSS. The inner structure of the cage is inorganic in nature and is externally covered by the organic substituent. Total number of silicon atom in this structure can be eight, ten or twelve. 2.2.2 Properties of POSS POSS molecules are physically large (3-4nm) with respect to polymer dimensions. However, it can be thought of as the smallest particle of silica possible. They can be viewed as molecular silica. Unlike silica, it contains reactive functionalities that are covalently bonded with the molecule. These functionalities can undergo polymerization or grafting to the polymer chains and make POSS compatible with others like polymers, biological systems and surfaces. Mono-dispersed size, low density, thermal stability and controlled functionality are some key features that have made POSS as one of the most utilized nano building blocks in constructing materials (Ying-Ling et al., 2008). National University of Singapore 10 Chapter Two: Literature Review R O R O O R Si O OR O Si Si R Si O Si R O Si O O Si O O Si R R Figure 2.2 Schematic diagram of POSS (3-4nm) POSS chemicals do not release any organic compounds, so they are odorless and environment friendly. The eight polymeric groups (R) at the eight corners can be of similar or different functionality. Moreover, all the corners may not contain reactive functional groups. This means that the groups can be specially designed to be reactive or nonreactive. Properties of POSS largely depend on these functional groups. Depending on the organic group’s reactive functionality, POSS can be classified as non-functional, mono-functional and poly functional. Each POSS molecule contains reactive-nonreactive organic functionalities for solubility and compatibility of the POSS segments with the various polymer systems. POSS has the ability to control the motions of the chains while maintaining the process and mechanical properties of the base resin when used as additives. The integration of POSS derivatives as an additive in polymeric materials dramatically improve the properties like thermal and oxidative resistance, mechanical properties, surface hardening as well as reduce flammability, heat flux and viscosity during processing. National University of Singapore 11 Chapter Two: Literature Review 2.2.3 Researches’ on POSS as an Additive The inclusion of POSS into other polymers has created the opportunity to build up high performance materials that unite many striking properties of both organic and inorganic components. Mostly, POSS has been used as additives -and can be successfully incorporate into the polymer by blending, copolymerization or grafting. The derivatives of POSS exhibit wide chemical versatility and good compatibility with organic materials as they possess organic substituents. The main reason of adding POSS as an additive is to improve the thermal and mechanical properties. As an additive, it can also act as viscosity modifier, dielectric constant modifier, cross-linking agent, flammability and fire retardants. Seckin and Koytepe (2008) have reported that amino functionalized octafunctional POSS was introduced into star polyimide by in situ curing to achieve low dielectric constant. This POSS-NH2 restricts the rotation by multiple point attachment to polyimide backbone. It is reported to lower down dielectric constant by introducing free volume into the film. Jieh-Ming et al. (2009) have studied the thermal property of poly benzoxazine/POSS hybrid nanocomposite and found that both glass transition and thermal degradation temperature have raised higher than the pristine poly benzoxazine. They have concluded that POSS cage effectively hinder the polymeric motion that results higher thermal stability. POSS derivatives also have several advantages over conventional fillers, including mono-dispersity, low density, high thermal stability and controlled functionality. Moreover, POSS monomers can be directly blended or copolymerized with other National University of Singapore 12 Chapter Two: Literature Review monomers to form polymers or nanocomposites. And thereby it is very simple and easy to study the effect of adding POSS derivatives. Reactive POSS can be introduced into the polymers via copolymerization, grafting and blending processes (Janowski and Pielichowski, 2008). Mostly, POSS is used to synthesize nano-composite materials with better performance. Nano-composite materials are attracting much attention as they possess enhance mechanical, thermal, optical and controlled dialectical properties which are usually achieved by the method of nano-reinforcement, the nanointerface, the synthetic procedure, the structural effects and the introduced interaction between polymeric organic and inorganic phase. Wahab et al. (2008) have suggested hydroxyl terminated POSS as a nanoscale building material to improve thermo-mechanical and dielectrical properties of polyimide due to its molecular scale and homogeneous distribution. For synthesizing hybrid nanocomposite by POSS, the judicious choice of functional group may change dispersion behavior of POSS in polymer matrix, enhance the processing parameters and impart desire thermo and mechanical properties of the hybrid materials. Usually the polymer-POSS nanocomposite materials are synthesized by copolymerization method. Copolymerization is the most common approach for enhancing the flammability, crystallinity and thermal, and polymer mechanical stability. Zucchi et al. (2009) have emphasized on the phase separation behavior National University of Singapore 13 Chapter Two: Literature Review after incorporating POSS into isobutyl methacrylate. Due to thermodynamic incompatibility, POSS-rich and polymer-rich domain have been formed which is undesirable. POSS nano cages can disperse at molecular level and act like micro dispersed filler which reduces the phase separation and enhances the thermo mechanical properties. Thermo mechanical properties are highly related to the microstructure which in turn depends to the interaction between the molecules. Polymer modifier requires control over phase separation in order to stop formation of additive rich and polymer rich domain. This is mainly controlled by the amount of additives used. In the study of the group Sanchezat-Soto et al. (2009), they have showed that good dispersion achieved up to 0-5wt% POSS derivatives as additives. For higher loading, phase separation usually occurs easily that degrades the thermo-mechanical properties. Significant decrease in surface energy took place for both linear and cross-linked polymer when POSS domain enriches on the surface. POSS is also used to synthesize the di-block polymer by controlled or living radical polymerization (Pyun and Matyjaszewski, 2001) as it allows greater controlled over molecular weight, topology and composition. Various approaches were taken to prepare hybrid copolymers, nano-particles, polymer brushed from POSS. POSS is also used as plastic material in medical, packing and coating, electronics, optical plastics etc. Another use of POSS is as a pre-ceramics in ablative materials, cladding and electronics coating and precursors to ceramic National University of Singapore 14 Chapter Two: Literature Review materials. With biodegradable and biocompatible functional group, POSS can also be used in medical application including release systems, implant, scaffold and tissue engineering (Hany et al., 2005). 2.2.4 Applications of POSS as Non-additive Other than using as an additive, researches on POSS are now spreading in different fields like simulation study on molecular POSS, medical applications, improvement of electrolyte properties for both fuel cell and lithium batteries and even as a space survival material. Polyimides such as Kapton® are used in spacecraft thermal blankets, solar arrays and space inflammable structures. One of the major problems with these Polyimides is the degradation due to the presence of oxygen. Use of silica enhances the oxidative resistance. However, imperfect coating of silica leads to Kapton® erosion. Several studies have (e.g., Tomczak, 2004; Tomczak et al., 2006) reported that POSS/ Kapton® polyimide enhance the oxidative resistance without costing other properties of Kapton®. There are a few studies on POSS related to the effects on the properties of dental composites such as mechanical strength, flexural strength, modulus and tensile strength. Wheeler et al. (2006) have studied the thermo mechanical properties and found that with 20 to 30% resign, significant improvement in the mechanical property achieved. Striolo et al.(2007) has studied the aggregation behavior of POSS monomer in liquid hexane with the help of molecular simulation. By using simulator they have National University of Singapore 15 Chapter Two: Literature Review obtained effective pair potential at dilute condition, short range effective repulsion, and side radial distribution in hexadecane. However, no experimental data’s are currently available to validate these results. Another recent use of POSS is to improve the properties of different membranes used as electrolyte. Incorporation of POSS derivatives into chitosan enhances the diffusivity of all amino acids and this property reduces with the longer tether of POSS derivatives (Tishchenko and Bleha, 2005). Another group (Zhang et al., 2007) studied the effect of POSS in the solid electrolyte for lithium batteries. They blend poly (ethylene oxide) derivative of POSS with high molecular weight poly (ethylene oxide) as solid polymer electrolyte and found that addition of POSS increase the conductivity at low temperature and it is limited to certain amount of POSS. However, the improvement is not appreciable above Tm. Polymer electrolyte membrane is used for proton exchange membrane (PEM) in fuel cell. Recent studies have also been focused on the improvement of this membrane while using POSS to improve the stability. Mostly Nafion is one of the polymeric membrane usually use in fuel cells. However, it has some drawbacks like methanol permeability which greatly reduces fuel cell’s performance. Derivatives of POSS are incorporated into Nafion and the effects are being studied by several researchers. Some studies (e.g., Zhang et al., 2009) have showed that POSS derivatives restructure the proton conducting channels inside National University of Singapore 16 Chapter Two: Literature Review the Nafion. The restructuring reduces methanol permeation that results higher power generation than pristine Nafion. Another drawback with Nafion is that it performs poorly in high temperature and low humid condition. Decker et al. (2009) have synthesized a multilayer PEM with sulfonated POSS and showed that this membrane performs well even at low humid condition. In their work, PEM is consists of an outer layer of sulfonated polyphenyl sulfone (S-PPSU) and the inner layer blend of octa-sulfonated octaphenyl POSS and S-PPSU. This membrane has excellent physical properties when compared with Nafion and have outstanding conductivity at 25% RH at 90°C. Yen et al.(2010) have prepared a new type cross-linked composite membrane from sulfonated poly (ether ether ketone) and POSS moieties. The membrane with sulfonated POSS moiety is reported to have higher selectivity, lower methanol permeability and relatively higher proton conductivity. Some other works have also been reported about the incorporation of POSS moiety in different PEM that greatly improve the thermo mechanical property as well as proton conductivity. 2.3 Dendrimer Dendrimer synthesis is relatively a new field of polymer chemistry. Dendrimers are highly branched, mono-dispersed macromolecules. They are defined by regular, highly branched monomers leading to a mono-disperse tree-like or generational structure. Synthesizing mono-disperse polymers demands a high National University of Singapore 17 Chapter Two: Literature Review level of synthetic control which is achieved through stepwise reactions, building the dendrimer up one monomer layer, or "generation," at a time. Dendrimers are nanostructures that carries molecule encapsulate to the interior void space or attached to the surface. Dendrimers are constructed through a set of repeating chemical synthesis procedure and are characterized by their structural perfection in terms of both uniformity and polydispersity. The size of dendrimer is about 1 to 10 nm. Figure 2.3 Dendrimeric structure The dendrimeric structure consists of a core, branching points and the terminal groups. Dendrimers of lower generations (0, 1 or 2) have highly asymmetric shape and possess more open space compared to the higher generations. In higher generation (generation 4 or more) the dendrimer adopt globular structure and National University of Singapore 18 Chapter Two: Literature Review become densely packed as they extent out to the periphery. The dendrimer diameter increases linearly whereas the number of surface groups increases geometrically. Dendrimer can be synthesized in both convergent and divergent method. In divergent method, the molecule is constructed from core to the periphery; whereas for convergent method, the dendrimer is synthesizes from outside and terminated at the core. Due to its structure, dendrimers have two chemical environments: the surface chemistry due to the functional group which surface to the dendritic sphere and the spheres interior which is mainly shielded from the external environment due to the dendrimer structure. The existence of those two distinct environments implies many possible applications for dendrimer. POSS structure can be considered as a dendrimeric core from where branches with desired functionality can be grown. The benefit of this approach is that higher number of desired functional groups (terminal groups) can be achieved that can drastically change the phase behavior. 2.4 Fuel cells (FC) Fuel cells are considered as one of the most promising technology for the power source of the future. Though the concept of fuel cell was first introduced in 1839 by Sir William Grove, it has emerged as a potential field in recent decades as an attractive electrical generation technology with least pollution. A fuel cell is an National University of Singapore 19 Chapter Two: Literature Review electrochemical energy conversion device. It directly converts the chemical energy to the electrical energy with high efficiency. Fuel is charged in anodic side and oxygen in the cathode side. The electrolyte acts as separator between fuel and oxygen to prevent direct combustion via missing. Therefore, electrons are conducted to the load via the external circuit. 2.4.1 Fuel cell theory A fuel cell consists of an electrolyte as a conductor of the charged particle, an anode and a cathode. In a hydrogen fuel cell, when the cell is activated by catalyst, the hydrogen gas separates into proton and electron. Electrons are conducted to the load through the wire whereas, proton pass through the electrolyte to the cathode to combine with oxygen to form water as waste. Two main electrochemical reactions occur in a fuel cell at the anode and cathode respectively. Anode half reaction: H 2 = 2 H + + 2e − Cathode half reaction: 1 O2 + 2 H + + 2e − = H 2 O 2 Overall reaction: H 2 + 1 O2 = H 2 O; ∆G = −273KJ / mol 2 National University of Singapore 20 Chapter Two: Literature Review Load Electrons folw round the external circuit Fuel Cathode Electrolyte Anode Oxygen Water Figure 2.4 Fuel cell diagram where ∆G is the change in Gibbs free energy of formation. The product of this reaction is water released at cathode or anode depending on the type of the fuel cell. The theoretical voltage E0 for an ideal H2/ O2 fuel cell at standard condition is 1.23 V. 2.4.2 Classification of fuel cells The fuel cells are classified according to the electrolyte as the electrolyte is defines the key properties of a fuel cell, particularly the operating temperature. Generally, there are six different types of fuel cell namely hydrogen fuel cell (HFC), direct methanol fuel cell (DMFC), phosphoric acid fuel cell (PAFC), alkaline fuel cell (AFC), molten carbonate fuel cell (MCFC), and solid oxide fuel cell (SOFC). Since HFC and DMFC use polymeric proton exchange membrane as electrolyte, those two are usually considered as proton exchange membrane fuel National University of Singapore 21 Chapter Two: Literature Review cell (PEMFC). Table 2.1 shows some important properties of different fuel cell types. Table 2.1 Classification of Fuel Cell Fuel cell type PEMFC PAFC AFC MCFC SOFC Electrolyte Polymer membrane Liquid H3PO4 Liquid KOH Molten carbonate Ceramic Charge carrier H+ H+ OH- CO32- O2- Operating Temperature 50-120° C 200° C 60-200° C 650° C 600-1000° C Catalyst Platinum Platinum Platinum Nickel Perovskites Cell components Carbon based Carbon based Carbon based Stainless based Ceramic based Fuel compatibility H 2, methanol H2 H2 H2, CH4 H2, CH4, CO Among those fuel cell options, PEMFC is the most promising option due to the high power density, rapid response to varying load and low operating temperature. However, some limitations like CO poisoning of the catalyst, expensive novel metal catalyst and methanol crossover make it necessary to look for suitable solution. Solid alkaline fuel cell (SAFC) could be another promising alternative for power generation. 2.4.3 Solid Alkaline Fuel Cell The primary component of a SAFC is similar with the PEMFC: an ion conducting electrolyte membrane, a cathode and an anode. The basic cell consists of a hydroxyl conducting membrane sandwiches between two platinum impregnated porous carbon electrodes. Together these three are often referred as membrane electrode assembly (MEA). A fuel such as hydrogen is charged into the anode National University of Singapore 22 Chapter Two: Literature Review compartment and an oxidant typically oxygen is placed into the cathode compartment. In contrast to the PEMFC, hydroxyl ion passes through the membrane form cathode to anode chamber. Load 2e - 2e- H2 ½ O2 A n o d e 2H2O 2 OH- C a t h o d e Anion Exchange Membrane Figure 2.5 Diagram of Solid alkaline fuel cell The electrokinetics of oxygen reduction in an alkaline medium is much enhanced in comparison with an acid medium which yields higher power densities and leads to higher efficiency for such systems. It also leads to the use of non precious catalysts. The following cell reaction shows it clearly: Anode : H 2 + 2OH → 2 H 2 O + 2e − Eo= -0.83 V Cathode : 0.5O2 + H 2 O + 2e − → 2OH − Eo = 0.40 V Overall cell reaction: H 2 + 0.5O2 → H 2 O Eo = 1.23 V National University of Singapore 23 Chapter Two: Literature Review 2.4.4 Anion Exchange Membrane (AEM) Anion exchange membrane is one of the possible solutions for power generation instead of proton exchange membrane. Though proton exchange membrane such as Nafion has been extensively studied and is promising candidate for portable power sources, it encounters some serious problems such as: 1) slow electrode kinetics, 2) CO poisoning at Pt electrode at low temperature, 3) methanol crossover that reduces the performance, and 4) high cost of the catalyst, membrane, separator, and so on. High volatility and toxicity of methanol may cause problems when used as portable power source. AEM could be a better way out to remove these limitations. In alkaline medium the electrode kinetics are faster and can be operate with non precious metal electrode such as silver and perovskite type oxides. These metals are inexpensive as well as tolerant to methanol. At high pH anionic exchange membranes can potentially eliminated or reduced the need of platinum based catalysts; thereby allowing cheaper metal catalysts and also improve the electrochemical kinetics. However, these hydrocarbon membranes are not as chemically stable as perfluorinated membrane. At high pH, nucleophilic attack take place (Ogumi et al., 2005) at the cationic sites which reduces the stability of the hydrocarbon based solid polymer anion exchange membrane. The atmospheric CO2 helps to start carbonation resulting decreasing the pH value of the solution and reactivity of the cell. National University of Singapore 24 Chapter Two: Literature Review 2.4.5 Studies on AEM Recent studies have mostly been focused on the preparation and modification of anion exchange membrane. There are several routes to prepare anion exchange membrane (Vinodh et al., 2010); (a) polymer blended with alkali, (b) pyridinium base type polymer, (c) radiation grafting and quaternization of polymer, and (d) chloromethylation and quaternization of polymer. Among these methods, the last one is the most advantageous and important one as it provides good physical stability as well as relatively high chemical stability. Most of the research on anion exchange membrane is about hydroxyl conducting membrane. Still there are few works on other type of anion such as carbonate ions. Unlu et al. (2009a) have compared between hydroxyl and carbonate conducting ions on fuel cell operation at about room temperature. In their study they have used poly (arylene ether sulfone) polymer membrane functionalized with quaternary ammonium cations and found that maximum power density is achieved with carbonate (4.1mW/ cm2) as conducting ions. Experimental analysis shows that CO2 is involved to oxygen reduction reaction and transported from cathode to anode resulting cell performance. However, their study did not mention about the performance at higher temperature. Another study (Vega and Mustain, 2010) also confirms that adding CO2 in the cathode stream of an anion exchange membrane of fuel cell increase the cell performance. A series of crosslinked quarternized poly vinyl alcohol (PVA) membrane was prepared by Xiong and his group (Xiong et al., 2008). National University of Singapore These membrane have reasonable 25 Chapter Two: Literature Review conductivity {(2.74- 7.34) × 10-3 S/ cm2} at room temperature. More notably these membranes have lower methanol crossover compared to Nafion and thus methanol permeability decreases with increasing concentration. Fung and Shen (2006) have reported that hydroxyl ion exchange membrane exhibits stability at higher temperature (below 150°C) with moderate performance. Recently, anion exchange membrane has been used to enhance the stability of power generation of single chamber microbial fuel cell (Yinghui et al., 2009). A decrease in power density is observed in cation exchange microbial fuel cell. To improve the stability of the cell, anion exchange membrane has been used and found 29% dropping in power density in 70 days which is 48% smaller than cation exchange membrane. This is mainly due to the difference of internal resistance development. In anion exchange membrane, lower amount of salt precipitates on cathode surface (comparing to cation exchange membrane) and results in less increase in cathode resistance that holds the stability of the microbial fuel cell. Jung et al. (2007) have studied the power generation effect in two chamber microbial fuel cell by using cation, anion and three types of ultra filtration membranes. Among those, AEM produces maximum power density due to proton charge transfer facilitated by phosphate ion and lower internal resistance. National University of Singapore 26 Chapter Two: Literature Review 2.4.6 Fuel Electrolyte Assembly for AEMFC Different types of fuels are used in AEMFC. Among those methanol, ethanol and H2 are mostly used fuels. Literature shows different types of membrane as electrolytes in AEMFC. Quaternized amine is mostly used as charge carrier in membrane. Zhang et al. (2008) have reported that aqueous ammonium borate is a potential fuel for AEMFC and can achieve up to 110 mW/cm2 power at 25°C. Ogumi et al. (2005) have used four polyhydric alcohol and methanol as fuel (in different study) in polyolefin chain containing Tetra alkyl ammonium membrane. The conducting ion was hydroxyl ion that generated about 9.8 mW/cm2 of power while using ethylene glycol as fuel at 25°C temperature. Fujiwara et al. (2008; 2009) have prepared hydroxyl conducting membrane contain quaternary amine. Then the membrane has been tested using ethanol and D-glucose in KOH solution as fuel. Unlu et al. (2009a) have compared conducting ion between OH- and CO32- with hydrogen gas as fuel and concluded that carbonate ion as conducting ion results higher power density as CO2 is related to oxygen reduction reaction at cathode. Hou et al. (2008) have prepared and studied alkali doped polybenzyl imidazole membrane using 2M methanol as fuel and found this membrane has a high thermal stability, acceptable mechanical properties, and less methanol crossover compared to Nafion. The following table shows some of the electrode fuel assembly from the literature. National University of Singapore 27 Chapter Two: Literature Review Table 2.2 Different types of Anion Exchange Membrane electrolyte assembly Conducting ion Power density mW/ cm2 Aq. ammonium borane (NH3BH3) OH- ~110 Polybenzimidazol doped in KOH Methanol OH- 31 polyolefin chain containing Tetra alkyl ammonium Four Polyhydric alcohols and CH3OH solution OH- 9.8 Electron-beam grafted ETFE H2/O2 and methanol OH- 130 Poly (arylene ether sulfone) containing quaternary amine Humidified Hydrogen gas OH- / CO32- 2.1 / 4.1 Quaternary amine blend with anion exchange resin ethanol OH- 58 Quaternary ammonia in ionomer solution D-glucose in KOH solution OH- 20 Membrane Anion exchange membrane Fuel Several studies have reported the performance of the membrane in various conditions like different types of fuel, concentration of fuels, electrolyte, membrane electrode assembly, operating conditions and so on. Therefore it is quite challenging to compare among these membranes. A novel type of hybrid fuel cell was prepared by using both PEM and AEM in such way that at least one electrode operates at high pH (Unlu et al., 2009b). The operation was conducted at steady state condition with a temperature of 65°C and zero relative humidity. Both the AEM/ PEM junction and fuel cell (AEM cathode/ PEM anode and PEM cathode/ AEM anode) have been evaluated and the operating behavior at the AEM/ PEM junction has been studied. Regardless of the configuration of the hybrid cell, the junction potential is about 1.23 Volts. AEM cathode and PEM anode offers viable configuration compared to PEM fuel cell National University of Singapore 28 Chapter Two: Literature Review only. They have also demonstrated that the overall performance of the hybrid cell is modest comparing to the PEM fuel cell. Kim et al. (2010) have reported the usage of anion exchange membrane in air breathing direct methanol fuel cell. Fuel cell designed to breathe air without any auxiliary pumps or fans can offer several advantages such as simplicity and more compact design. The electrochemical performance of this membrane strongly depends on the concentration of methanol in KOH solution. Further work is necessary to use this membrane effectively. 2.5 Summary This chapter has presented a brief introduction of POSS and Anion Exchange Membrane and reviewed literatures on the application of POSS. Attracting features and general research areas of POSS have been discussed in an ordered fashion. An extensive description on the property modification of various polymers has also been presented by adopting different functionality of POSS. Use of POSS in different fields other than additive has been reviewed as well. Literature suggests that behaviors of POSS have not been explored well. In particular, colloidal behaviors of POSS in different medium seem to have a great potential. Since POSS is a nano structure material, a fuller understanding on its colloidal behaviors would be helpful to apply such material in numerous directions. Another potential gap identified by literature reviews is the anion exchange membrane. Anion exchange membrane is considered to be an National University of Singapore 29 Chapter Two: Literature Review alternative solution for Fuel cell to overcome the limitations with Proton Exchange Membrane. Synthesis of AEM is relatively a new idea and hence will be interesting and useful to look at. The experimental procedures as well as materials and equipments used to achieve the aim of this study will be elaborately discussed in the next chapter. National University of Singapore 30 CHAPTER THREE MATERIALS AND METHODS 3.1 Introduction This chapter describes experimental frameworks and materials that have been used in this study. Three different structures of POSS have been designed to synthesize from methacrylate-POSS derivatives and a polymeric membrane has been developed by using UV polymerization. The name of the chemicals and suppliers employed in the reaction steps are mentioned. Step by step reaction procedures are described along with the reaction conditions. At the same time product purification and collection procedure are also mentioned. To characterize and analyze the synthesized products, different types of characterization and analytical tools have been used. This chapter also presents a brief description of different equipments used in this study along with their manufacturers and model number. 3.2 Materials Polyhedral Oligomeric Silsesquine (POSS) was purchased from Sigma-Aldrich. Ethylene diamine was obtained from Merck Schuchardt. 2-Hydroxyethyl methacrylate (HEMA, 97%), glycidyl methacrylate (GMA, 97%), acrylonitrile (AN), vinyl benzyl chloride (VBC, 97%) were obtained from Sigma-Aldrich. N- National University of Singapore 31 Chapter Three: Materials and Methods methyl diethanol amine, copper (I) chloride (CuCl), 2, 2-Bipyridine (Bpy) were purchased from Alfa Aesar.DMPA as UV initiater, allyl bromide, acetonitrile (J.T. Baker, 99.9%), ethyl acetate (Scientific Fisher, 99.97%), N, Ndimethylformamide (DMF, AR grade from Merck) and methanol (Merck, 99.8%) analytic grade was used for synthesizing POSS molecules and membranes. Both Deionized (DI) water and Millipore water have been used in this study. 3.3 Experimental Framework Experimental procedures and reaction conditions of this study have been elaborately described in this section. Experimental materials for synthesizing of colloidal particles and anion exchange membranes have been introduced and discussed in subsequent sections. 3.3.1 Synthesis for colloidal study In this section, the reaction procedure for the colloidal part has been described. Three different structures of POSS have been synthesized and a comparative study of colloidal behavior has been carried out. All the structures of POSS are branched via dendrimeric reaction. POSS is considered as a dendrimeric core and these three structures have been constructed from the amine derivatives of POSS which can be denoted as generation zero (G0). The first structure is designed to carry two chains of 2-Hydroxyethyl methacrylate (HEMA) at each branching unit of G0. For the second structure, it carries the same chains together with a charged National University of Singapore 32 Chapter Three: Materials and Methods particle at each corner. For our study, the charged particle is quaternary amine. And the third structure is designed in such way that it has three branches of HEMA at each corner. Synthesis procedures of each structure are presented in following sections subsequently. 3.3.1.1 Synthesis of G0-HEMA with two branches The first structure is synthesized in two steps. The first step is identical for all the three structures. Here, the amine group is linked to the POSS to create the branching points. This structure is considered as G0. In the second step, HEMA is added to construct G0-HEMA with two branches. (a) Synthesis of amine-POSS (G0): amine-POSS was synthesized according to the procedures described to synthesis dendrimeric structure in literature (Tulu et al., 2009). 0.2 g (0.14 mmol) of methacryl substituted was added into large amount of methanol and was stirred for about an hour. 50% excess (0.11 ml) EDA was added in round bottom flux together with 30 ml of methanol. The whole system was kept in ice bath at 0° C and argon was purged through it. POSS-methanol solution was added very slowly while the mixture was vigorously stirred. After half an hour of the addition of the POSS solution, the ice bath was removed and the reaction was continued at room temperature for 24 h. Then the mixture was heated at 40° C for 12h. The reaction scheme is shown in figure 3.1. National University of Singapore 33 Chapter Three: Materials and Methods O O R R R RT, 24 h in methanol O R H3C R R R' CH2 H 2C + H2N R' POSS EDA NH2 CH3 R' R R NH O R' NH2 R' CH2 H2C R' Amine-POSS (G0) Figure 3.1 The scheme to prepare Amine-POSS (G0) The synthesized product was purified by washing in methanol in rotary evaporator under vacuum to remove the excess amine. This synthesized particle is considered as zero generation (G0). After purification, this product was used to carry out the next step to prepare the highly branched nanoparticle. (b)Synthesis of G0-HEMA with two branches: Same procedure was applied to graft 2-Hydroxyethyl methacrylate (HEMA) on G0 generation of POSS. G0 was dissolved in methanol and kept at 0° C in inert medium for half an hour. 0.3 ml HEMA in methanol solution was added slowly. Then the reaction was continued for 24h at room temperature followed by heating at 40° C for 12h. Purification was carried out using rotary evaporator under vacuum. The reaction scheme is shown in figure 3.2. National University of Singapore 34 Chapter Three: Materials and Methods O R' R' H2C R' NH NH2 O CH3 + C H2C R' R' R' CH2 O R C H2 O C RT, 24h in Methanol H2 C OH HEMA CH3 O ' H C H2C Amine-POSS O R'' R'' R R CH2 NH R'' OH CH3 N O R'' '' H2C '' H2 H2 O C C C O H2C CH CH3 C O H2 C H2 C OH CH3 R'' G0-HEMA O R' = H 2C CH2 NH NH2 O CH3 O H 2C O C H2 H2 O C C OH CH3 CH2 R''= H2C H C NH N O CH3 H 2C O H C C H2 H2 O C C OH CH3 Figure 3.2 The scheme to synthesize G0-HEMA with two branches 3.3.1.2 Synthesis of G0-HEMA with Quaternary Amine The quaternary amine was synthesized following the procedure mentioned in a recently published protocol (e.g., Chatterjee et al., 2002; Das and Das, 2003). Briefly, first structure and allyl bromide were taken in molar ratio 1:8 in mixed solvent (30% methanol/ 70% acetonitrile) and refluxed at 90° C for 24h. The product was obtained by crystallization and was washed in methanol. Figure 3.3 shows the scheme for the synthesis. National University of Singapore 35 Chapter Three: Materials and Methods H2C O R'' R'' R'' R'' O H2 C H2 C OH H2C + O H C H2C CH3 C CH3 N O R'' R'' CH2 NH H2C O H C C H2 C O C H H2C Reflux- 24h at 900 C Br 30% methanol/ 70% acetonitrile Allyl Bromide H2 C OH CH3 R'' O G0-HEMA H2C R''' R''' + CH3 N Br CH2 NH H2C H2C H C H2C CH3 R''' R''' R''' H C O R''' O H2C C O H2 C H2 C OH O H2 C H2 C OH O H C C CH3 R''' G0-HEMA with Quaternary Amine O H2C O R''= H 2C CH2 NH + N Br- O H2C CH3 H C H2 H2 O C C OH C CH3 O H C H2 H2 O C C OH C CH3 O R'''= H2C CH2 O CH3 H2C H2C H C + CH3 C N Br NH H2C C H O H2C H C O C H2 H2 O C C OH H2 H2 O C C OH CH3 Figure 3.3 Scheme for G0-HEMA with Quaternary Amine 3.3.1.3 Synthesis of G0-HEMA with three branches Third structure was synthesized similarly as the procedure of the second structure. Amount of HEMA taken for this reaction is 0.4ml (exactly 1: 3 ratio of POSS reactive corner to HEMA). The reaction was carried out for 1 day at room temperature followed by heating at 40°C for 12 hr in inert atmosphere. The product was crystallized into the reaction medium and was washed with methanol. Figure 3.4 shows the structure of the particle. National University of Singapore 36 Chapter Three: Materials and Methods O R' R' R H 2C NH2 O CH3 H 2C + R' R' R' NH CH2 O ' R C C H2 C O RT, 24h in Methanol H2 C OH HEMA CH 3 O ' H2 C amine-POSS RIV R IV H2 C RIV H2 C H2 C O O CH3 C C H H2 C RIV H3C R IV RIV O H2 C H2 C OH O H2 C H2 C OH N N H 2C CH H C O C CH 3 O C Target 3 C CH 3 CH2 RIV H C O H2 C C H2 OH O R'= CH2 H2 C NH NH2 O CH 3 H2C O R'v= H C O C O H2 C H2 C OH O H2 C H2 C OH CH3 H2 C CH2 N N CH2 H2C O CH3 C O H2 C O C CH3 CH CH3 O H C H2 C OH Figure 3.4 Scheme for synthesizing G0-HEMA with three branches 3.3.2 Synthesis of Anion Exchange Membrane Anion exchange membrane is also considered as di-block block membrane as it contains hydrophobic and hydrophilic part. The hydrophobic part provides the structure of the membrane and can be named as backbone. And, the hydrophilic part is mainly charged particles that help to pass ions through the membrane. The preparation of this membrane contains three experimental steps followed by membrane casting. The steps are described below: National University of Singapore 37 Chapter Three: Materials and Methods 3.3.2.1 Synthesis of the backbone The ratio of different monomers (VBC, AN, GMA) was selected to synthesize the backbone by trying different batch of polymerization reaction and looking at their membrane properties. Finally, VBC: AN: GMA were taken in 2.5: 92.5: 5 volume ratios in a glass tube. 5 ml of DMF was added with 2ml of these monomers together with 20mg of the initiator (DMPA). Argon gas was purged through the solution for 15 min. Then UV polymerization reaction took place for 2h. CH3 HC CH2 + HC CH2 + UV polimerization DPMA initiator,2h CH2 C C N H2C Cl VBC C O O H2 C O C H CH2 GMA AN CH3 H C H2C Cl H2 C x H C H2 C y H2 C C C C N O z O O H2 C CH Backbone Figure 3.5 Structure of backbone After UV polymerization the product was collected by precipitation into cold methanol-water (1:1) solution and was washed to remove the impurities. This product was considered as hydrophobic backbone for membrane preparation and stored for the next step after drying. National University of Singapore 38 CH2 Chapter Three: Materials and Methods 3.3.2.2 Preparation of the Charge Carrier Group Quaternary amine was used as a charge carrier group in this study. The preparation procedure has been adopted from a recent work (Das and Das, 2003). According to the procedure, 1 ml of allyl bromide and excess amount of N-methyl diethanol amine (5ml) were taken in 30% methanol/ acetonitrile solution (15ml) and refluxed at 90° C. After refluxing for 24h, the solvent was evaporated using rotary evaporator and was washed with ethyl acetate and kept inside the fridge to crystallize the product. OH OH H 2C H 2C C H + CH2Br H 3C Allyl Bromide Reflux 24h at 900C methanol:acetonitrile(3:7) N OH N-methyl diethanol amine C H CH 2 N+ BrH 3C OH Quaternary monomer Figure 3.6 Synthesis scheme of the cationic monomer The crystallized product was purified by washing with ethyl acetate and dried under vacuum. This quaternary amine acted as a monomer in ATRP reaction in the following step. 3.3.2.3 Grafting the monomer on to the backbone via ATRP Atom Transfer Radical Polymerization (ATRP) was employed to carry out the grafting of quaternary amine to the VBC group of backbone. A typical procedure National University of Singapore 39 Chapter Three: Materials and Methods for this synthesis is as follows: the backbone (0.7g), quaternary amine (36mg) and Bpy (70.2324 mg) were added into 10ml of DMF and the temperature was maintained at 40°C. After 30 minute, calculated amount of CuCl (14.815 mg) were added and the reaction medium was charged with argon gas. CH3 H2 C H C H2 C H C x H2 C y C C C z O O H2 C O N + CH OH H2C C H N+ Br CH 2 H2C Cl Backbone (initiator) H2 C H C x H3C OH monomer CuCl/Bpy in DMF 12h, 400C CH3 H C CH2 H2 C C y H2 C N C C O z O H2 C O CH CH2 H2C C H CH n Cl OH H 2C N+ Br H3C OH Figure 3.7 Growing Quaternary Amine chain on Backbone After 12h, sample was precipitated into methanol water (v:v=1:1) mixture and washed with methanol to remove the impurities. 3.3.2.4 Membrane casting A calculated amount of cation grafted polymer obtained from the ATRP was introduced into DMF (10ml) and mixed uniformly with the aid of ultrasonication. Required amount of the cross-linker (either EDA or Amine-POSS) was added into National University of Singapore 40 Chapter Three: Materials and Methods the solution to open the epoxy ring of GMA. The resulting solution was cast in a Teflon Petri-dish (d= 8cm). The dish was kept into the furnace at 80° C for 8h to generate composite membrane. After casting, the membrane was washed with DI water and stored in hydrated condition for further analysis. 3.4 Characterization and Analytical Tools After synthesizing the materials, characterization need to be done to confirm the exact structure of the product. Once the product is confirmed, colloidal behavioral analyses and membrane performance evaluations have been conducted. This part gives a brief description of the characterization and analytical tools and the conditions under which the study has been done. 3.4.1 Fourier Transform Infrared Spectroscopy (FTIR) FTIR measurements of all samples had been performed using a Shimadzu infrared spectrometer (Model 400) with KBr as background over the range of 4000-400 -1 cm . 3.4.2 Transmission Electronic Microscopy (TEM) A bright-field TEM (JEM-2010) was used to measure the size of branched polymeric particles. The sample was prepared by coating a thin layer of diluted sample suspended on methanol on a copper grid (200mesh and cover with National University of Singapore 41 Chapter Three: Materials and Methods formvar/carbon). The copper film was then dried at 50°C for few hours before the measurement. 3.4.3 Thermogravimetric Analysis (TGA) The thermogravimetric analysis was performed on a thermal analysis system (Model: TA 2050). For TGA measurements, the mass loss of dried sample was monitored under N2 at temperatures from room temperature to 850°C at a rate of 10°C/min. 3.4.4 Scanning Electric Microscope (SEM) The morphology of the membrane was analyzed using SEM. The sample was prepared by coating the suspended sample on the carbon tape of the sample holder. The sample was then dried at 50°C for few hours and coated with platinum before measurement. 3.4.5 Dynamic Light Scattering (DLSC) Size distributions of the colloidal particles were measured using DLSC. Samples were prepared at different pH using water as the solvent. For each sample, six runs were conducted and the average was taken as the result. National University of Singapore 42 Chapter Three: Materials and Methods 3.4.6 Auto Lab The logarithms of the impedance of membranes at different temperature were measured by AUTO LAB. The measurement was carried out in potentiostatic mode over a frequency range from 10Hz to 1MHz with an oscillating voltage of 5mV. The four probe membrane holder was prepared in the laboratory. The sample was dipped into KOH for 24 hours and heated at 60º C for 30 min before the measurement. Then the conductivity, σ (S/cm) was calculated by following equation. Conductivity (σ) = l RA where l is thickness of the membrane (cm), A is the membrane surface area exposed to the electric field (cm2), and R is the membrane resistance (Ω). 3.4.7 Particle Size Analyzer (Zeta Sizer) Malvern instrument of zeta sizer was used to find out the charging behavior of the colloidal particles at different pH and also in presence of different charging ions. Mainly water was used as dispersant phase. In some cases, mixer of water and ethanol (about 1~2%) was used. 3.4.8 Gas Chromatography (GC) Shimadzu GC was used to measure the crossover of the membrane. The method used for analysis was made for methanol and ethanol crossover. National University of Singapore 43 Chapter Three: Materials and Methods 3.4.9 UV Cross-Linker UV cross-linker (lab made) was used to polymerize the monomers under UV light for a certain period of time. Polymerization reaction took place for 2 hours and the number of light source used to undergo the polymerization reaction was 12. 3.5 Summary This chapter gives an overall idea of materials and methods employed in the study. The experimental procedures of all the three structures from POSS have been described extensively. Brief descriptions of analytical tools have also been presented. Characterization tools such as IR, NMR, and TEM are used to analyze the structures from POSS. Dynamic light scattering, particle size analyzer are occupied to study the colloidal behavior of those particles. Anion exchange membrane was prepared in four steps. A comprehensible description of these steps has been provided. In the first step, the polymeric backbone was prepared by UV polymerization that gave the structure of the membrane. In second step, the charge carrying part (quaternary amine) was prepared followed by the grafting of the charged part to the backbone via ATRP reaction in the third step. Finally the membrane was cast using two cross-linkers in a Teflon dish. Analytical tools were used to characterize and to evaluate the performance of the membrane. The results are shown and discussed in the following chapter. National University of Singapore 44 CHAPTER FOUR RESULTS AND DISCUSSIONS 4.1 Introduction This chapter presents the characterization techniques used to analyze the structure of the desired products. For the structures of POSS derivatives, NMR and FTIR were done in order to have a rough idea about the structure. TEM pictures were taken to show the differences between the three structures. The structure of the membrane was analyzed using IR, TGA and EDX. Effects of pH on particle size and surface charge were studied for three structures of POSS while suspending into water medium. Studies were also carried out for boron adsorption at higher pH level. The kinetic study of G0-HEMA with two branches was conducted by measuring the contact angle with water at different hydrophobic medium and relative humidity to understand the surface behavior when coated onto a glass surface. Polymeric membranes were constructed using two types of cross-linker in the same membrane materials. In the first case amine was used to open the epoxy groups whereas in the second case amine-POSS was used. Water uptake, conductivity and methanol crossover were measured to evaluate the performance of each membrane and a comparative study was done between the membranes using two separate cross-linkers. National University of Singapore 45 Chapter Four: Results and Discussions 4.2 Characterization of POSS derivatives Three derivatives of POSS were developed in this study. Characterizations of those POSS derivatives are shown in the following section. To confirm the structural configuration FTIR and 1H-NMR were conducted. TEM pictures were taken to taken to understand the agglomerating nature of the particles. 4.2.1 Characterization of G0-HEMA with two branches The reaction pathway was verified by FTIR studies. Figure 4.1 shows the FTIR spectra of POSS, ethylene diamine grafted POSS (G0) and HEMA grafted G0. c b OH & NH stretching 1731 a NH stretching 2983 Si-o-Si stretching 4000 3600 3200 2800 2400 2000 1600 1200 800 400 cm-1 Figure 4.1 FTIR spectra of (a) POSS, (b) POSS-amine (G0) and (c) G0HEMAwith two branches. The functional group of the POSS used in this research - was the propylmethacryl group. The characteristic IR peak for siloxane bond is in the region of 1100-1000 cm-1. The strong peak in 1720 cm-1 region is due to the carbonyl group in POSS. National University of Singapore 46 Chapter Four: Results and Discussions The change in intensity (1731 cm-1) of carbonyl peak amine-POSS (G0) confirms the breaking of the C=C double bond due to the reaction as the conjugated double bond appears at the lower energy (higher wave number) region. The peak at 2983 cm-1 in POSS is due to C-H stretching vibration of alkene group (C=C) which is reduced in both POSS-amine (G0) and G0-HEMA with two branches. This confirms the arms of POSS undergo reaction with amine. The broad peak appeared at 3200 cm-1 to 3400 cm-1 is due to the presence of secondary amine in the sample. For G0-HEMA with two branches, slightly broader peak has been found for hydroxyl group and secondary amine around 3200 cm-1 to 3400 cm-1 region. From the results of FTIR, the dendrimeric structure of POSS has been confirmed. a R'' R'' Si O O Si O O O R'' Si O Si O R'' O CH2 Si H2C O R'' Si O b O R'' NH H2 C f O C H2 O C H2 C OH H2 O C H2 C OH CH3 d H3C H C N H2C c O Si O O Si e H C O C g CH3 R'' e, f c d, g b a Figure 4.2 1H-NMR of G0-HEMA with two branches In order to figure out the number of HEMA branched grafted on the POSS, 1HNMR has been done. The peaks for the corresponding H are shown in figure 4.2. National University of Singapore 47 Chapter Four: Results and Discussions The presence of d, e, f, g peaks on the spectrum confirms the grafting of HEMA. However, the number of branches is not the same as expected. The ratio between the hydrogen with oxygen and nitrogen molecule (peak g and d) and the hydrogen with carbon molecule (peak b) is about 1. This suggests that roughly one chain has been grafted at each corner of POSS. a 10-20nm ~50 nm b Figure 4.3 Transmission electron micrograph of (a) POSS and (b) G0-HEMA with two branches TEM pictures have also been carefully observed to identify the difference between POSS and G0-HEMA structure. Typical TEM micrographs of POSS and the dendritic structure are shown in figure 4.3. However, it is quite difficult to trace individual particles. Therefore from TEM images, the smallest size has been taken as an individual particle. It shows that the particle size of POSS is about 10 to 20 nm whereas the corresponding size for G0-HEMA increases to 50 nm. The increase in size of particles is due to the presence of HEMA chain in POSS structure. National University of Singapore 48 Chapter Four: Results and Discussions 4.2.2 Characterization of G0-HEMA with Quaternary Amine The formation of the quaternary amine is not possible to confirm by using the IR spectrum as there is no characteristic peak for quaternary salt (Socrates, 2001). For this reason the IR spectrum of G0-HEMA with quaternary amine carries less importance. Hence the spectrum has been shown in figure 4.4. 3900 3400 2900 2400 1900 1400 900 400 cm -1 Figure 4.4 IR spectrum of G0-HEMA with Quaternary Amine Like TEM images of first structure, individual particles of G0-HEMA with quaternary amine are also difficult observe from images. Compare to the first structure, these particles have a higher degree of agglomeration. The first structure is branched POSS particles with OH terminated group (G0-HEMA) while second one contains quaternary amine group with previous structure. The counter ion is chloride ion which acts as a bridge to bring the particles closer. In other words, due to the presence of charge, intra-particle attraction took place that National University of Singapore 49 Chapter Four: Results and Discussions results in strong aggregation. The TEM picture of the solid particle is shown in figure 4.5. Figure 4.5 TEM image of G0-HEMA with Quaternary Amine 4.2.3 Characterization of G0-HEMA with three branches The IR spectrum of this particle is exactly the same with the G0-HEMA with two branches (i.e., first structure). This is quite natural as both of these particles have same functional groups. Moreover, from 1H-NMR it is evident that grafting of HEMA branches is hampered by the static hindrance of POSS. As a result, there are no significant differences between G0-HEMA with two and three branches. 4.3 Study of the Colloidal Behavior of POSS The size changes of three structures of POSS have been studied over a pH range from 2 to 11. This study has been done using dynamic light scattering (DLSC) method. Moreover the particle charge at different pH level has been estimated by measuring zeta potential. Adsorption of boric acid has also been measured at the same time. National University of Singapore 50 Chapter Four: Results and Discussions 4.3.1 Dynamic Light Scattering In this study, the pH effect on the particle size has been studied using DLSC keeping the concentration of particles on water medium as 0.37 mg/ml. Results show that particles of G0-HEMA with two branches exhibits oscillating behaviors with the change in pH range (see figure 4.6). While the particle size is about 574 nm at pH of about 2, the corresponding size reduces to 280 nm with an increase of pH to about 3. This is because this particle undergoes protonation at low pH and starts deprotonation with the increase in pH. Interestingly the particles have the highest size at pH about 8 due to the adsorption of hydroxyl (OH) group. Moreover further increase of the pH in the basic region, the particle size reduces due to compression of sodium ion. This phenomenon is also called ionic compression. Hydrodynamic behavior of G0-HEMA 1200 zero surface charge d(nm) 1000 800 protonation 600 ionic compression 400 deprotonation 200 0 2 4 6 8 10 12 pH Figure 4.6 Size distribution of G0-HEMA with two branches at different pH National University of Singapore 51 Chapter Four: Results and Discussions Size distribution of G0-HEMA with quaternary amine has been studied in water medium with a concentration of 0.1875 mg/ ml. Size distribution of G0-HEMA with quaternary amine at different pH level shows single-peaked distribution. Since this particle contains quaternary amine group, significant adsorption of proton does not take place at low pH due to its positive charge. While particle size is larger at about pH 5.5 for the adsorption of hydroxyl group, the size reduces at higher pH because of ionic compression. Hydrodynamic behavior of G0-HEMA with Quaternary Amine 1400 size (nm) 1200 1000 800 600 400 200 2 4 6 8 10 pH Figure 4.7 Size distribution of G0-HEMA with quaternary amine at different pH As observed from figure 4.8, G0-HEMA with three branches shows similar behaviors as if for those with two branches. However a slight shift of pH on hydroxyl ion adsorption has been noticed between those two. This may be due to the presence of slightly higher number of HEMA chain in the structure. National University of Singapore 52 Chapter Four: Results and Discussions G0-HEMA with three branches adsorption of OH groups 8200 7200 protonation size (nm) 6200 5200 4200 3200 2200 ionic compression deprotonation 1200 200 2 4 6 8 10 12 14 pH Figure 4.8 Size distribution of G0-HEMA with three branches at different pH Size of the particles of those three POSS structures has also been tested by adding different salts at different pH level. Experimental results show that the swelling behavior is quite stable for all three structures when different metal salts like CuSO4, and CuCl2 have been added at a concentration level of 15µg/L. 4.3.2 Zeta potential The effect of pH on zeta potential has been studied for G0-HEMA with quaternary amine and branches using water medium and 1-2% ethanol water solution respectively. The concentration of POSS particles used for this part is same as for size distribution study. As all the particles contain terminal hydroxyl groups, effect of adding Boric acid has also been studied. Previous studies showed that boric acid starts to dissociate into borate ion at pH > 9.2 and reaches to 100% borate ion at pH 12 (Glueckstern and Priel, 2007; Koseoglu et al., 2008; Nadav, National University of Singapore 53 Chapter Four: Results and Discussions 1999). At pH higher than 9.2, it can be removed by adsorbing on the surface containing hydroxyl groups (Geffen et al., 2006; Senkal and Bicak, 2003). H 3 BO3 → H 2 BO3 − Effect of pH and Boric acid on G0-HEMA with two branches with out Boric acid 80 Z (mV) with Boric acid 40 0 2 3 4 5 6 7 8 9 10 11 12 -40 -80 pH Figure 4.9 Effect of pH on G0-HEMA with two branches For G0-HEMA with two branches, particles have iso-electric point at about pH of 7.5. As observed from figure 4.9, adsorption of Boric acid does not take place. Similar experiment has been conducted for G0-HEMA with quaternary amine. This particle exhibits iso-electric point at pH of about 5.5 and shows a higher zeta potential in water medium that reflects its stability. Including Boric acid into the system reduces the value of zeta potential. At pH greater than 9.5 (see figure 4.10), there is a significant drop in zeta potential which indicates sufficient amount of borate ion has adsorbed on the surface and the value of zeta potential drops due to the low charge density of borate ion. Earlier researches (e.g., Jacob, 2007) have also reported that presence of quaternary amine enhance the borate National University of Singapore 54 Chapter Four: Results and Discussions ion adsorption. The surface adsorption may be expressed by the following OH relation: O B- + H 3 BO3 O OH Effect of pH and Boric acid on Quaternized POSS 30 without Boric acid Z (mV) wiith 30 ppm of Boric acid 10 with 50 ppm Boric acid -10 2 3 4 5 6 7 8 9 10 11 12 -30 -50 pH Figure 4.10 Effect of pH and Boric Acid on G0-HEMA with Quaternary Amine on zeta potential Z (mV) Effect of pH and Boric Acid on G0-HEMA with three branches 15 without Boric Acid 10 with Boric Acid 5 0 -5 2 4 6 8 10 12 14 -10 -15 pH Figure 4.11 Effect of pH and Boric acid on G0-HEMA with three branches on zeta potential National University of Singapore 55 Chapter Four: Results and Discussions G0-HEMA with three branches shows almost same behaviors when there were two branches. Figure 4.11 shows the zeta potential of third structure when using only water as disperse medium which results the smaller potential of the particle. Adding Boric acid almost has no effect on the surface charge of the particle. 4.4 Kinetics study A thin layer of G0-HEMA with two branches in methanol solution was coated on a glass substrate (22mm × 22mm) using spin coater at 2000 rpm for 60 sec. After that, methanol was evaporated at 35°C using vacuum. The kinetic behavior of coated layer on glass surface was studied at different solvent and relative humid condition. Figure 4.12 shows the optical image of the coated surface. The surface can be considered as uniformly coated though there is some uncoated area in micro range. Figure 4.12 Surface morphology of the coated layer National University of Singapore 56 Chapter Four: Results and Discussions 4.4.1 Kinetic study at different hydrophobic solvents After coating, the coated glass was immersed into three different hydrophobic solvents (Toluene, Cyclohexane, Ethyl acetate) for 30 minutes and dried under vacuum inside the desicator. After that the effect of moisture was studied when exposed to the atmospheric condition by measuring the contact angle with water. Figure 4.13 describes the preparation steps of the coated glass on a typical solvent. Figure 4.13 Coated glass at different hydrophobic medium. The study of the kinetic behavior was conducted by measuring the contact angle with time and at different conditions. Initially the hydrophobic part of the particle (cage structure) was oriented upside and assigned very high contact angle. After the coated substrate was exposed to the hydrophilic atmosphere with moisture, flip over of the hydrophobic part took place as the contact angle become smaller. National University of Singapore 57 Chapter Four: Results and Discussions Effect of hydrophobicity on kinetic behavior RH~ 50-60% 90 50 80 Contact angle Contact Angle 100 70 60 50 Cyclohexane Ethyl Acetate Toluene 48 46 44 42 40 0 0 10 20 30 40 2 4 50 6 8 10 Time (min) 60 Time (min) Figure 4.14 Study the effect of atmosphere on G0-HEMA coated layer. Reducing value of contact angle has been observed for both ethyl acetate and cyclohexane and presented in figure 4.14. Results indicate that the surface becomes more hydrophilic with time. The surface of the coated layer was initially hydrophobic and with the contact of the atmospheric moisture it turns out hydrophilic. Though cyclohexane is more hydrophobic than ethyl acetate, there is not much difference in the kinetic behavior. The flip over of the surface from hydrophobic to hydrophilic atmosphere took place within the first few minutes. Contact angle drops from 50º to 44º within first 10 minutes and for the later part the change is negligible. After two days it reaches 40°. Figure 4.14 shows how the surface changes with the effect of atmosphere. The surface behavior can further be explained by figure 4.15. Coating of G0HEMA makes glass surface hydrophilic. However the orientation of dendritic National University of Singapore 58 Chapter Four: Results and Discussions structure changes to a higher contact angle in the extreme hydrophobic condition because the hydrophobic core of the structures comes to the upper surface. Moreover exposure to the atmospheric condition leads the hydrophilic branches to come upside resulting in lower contact angle. HO OH OH OH HO HO in Hydrophobic In hydrophobic In EA condition solvent OH HO OH HO HO OH OH HO HO OH OH HO OH OH at atm condition RH=50-60 HO HO HO OH OH Figure 4.15 Effect of different atmospheric condition on the surface orientation However toluene does not show such trend even after a longer exposure (48 hrs) on the atmospheric condition. It can be deduced that contact with toluene build a meta-stable structure that resist any change in surface condition. This may be due to the longer contact duration with Toluene residue (due to high boiling point) that interacts with the layer and keeps it unaffected with further changes. 4.4.2 Kinetic study at different Relative Humidity Different relative humidity (RH) was created by using saturated salt solutions and the coated glass (after subjected to different hydrophobic solvents) was kept inside of that saturated environment for 24 hrs. The saturated salt solutions used National University of Singapore 59 Chapter Four: Results and Discussions to reach different relative humidity are Lithium Chloride (RH=11.3%), Magnesium Chloride (RH= 32.8%), Potassium Carbonate (RH= 43.1%), Magnesium Nitrate (RH= 53%), and Potassium Chloride (RH= 84.3%). When a coated glass was placed at different humid condition consecutively, no difference was noticed because of the surface rigidity for the longer time effect. To overcome this, a single coated substrate was placed in each relative humid medium. The contact angle was measured before and after placement into each humid medium. Table 4.1 shows the effect of different RH on the coated surface. Table 4.1 Effect of relative humidity on the surface structure Lithium Chloride, 11.3% In Ethyl acetate Before After 35.77±0.21 37.13±1.90 In Cyclohexane Before After 36.63±0.32 37.82±0.81 Magnesium Chloride, 32.8% 35.87±0.57 38.33±1.90 33.63±0.32 34.37±0.38 Potassium Carbonate, 43.1% 31.30±1.21 26.10±0.72 35.8±0.40 35.43±0.85 Magnesium Nitrate, 53% 38.87±0.91 33.4±1.90 35.93±0.41 29.52±0.41 Relative humidity From the table it can be said that the surface structure does not show much changes in lower humid condition. However at higher humid condition (like 43~53% RH), the contact angle become smaller due to the change in surface orientation. The kinetic study is very sensitive to the surrounding conditions. The results varies with the processing steps, time duration, presence of foreign particles, room temperature etc. Even higher relative humidity (saturated KCl salt, 84.3%) was also studied for both solvents. Results show an uneven track with some points shows higher National University of Singapore 60 Chapter Four: Results and Discussions hydrophobicity whereas some shows hydrophilicity. This may be due to the sudden effect of higher humidity that does not allow sufficient time for the surface orientation. The kinetic study of branched POSS reveals that this kind of structure undergoes flip over when it is subjected to hydrophilic atmosphere from hydrophobic one. This is mainly due to the orientation of the OH-tele branches of the POSS. Moreover this structure shows oscillating behavior of its size at different pH range due to the adsorption and desorption of proton on its surface. 4.5 Characterization of the membrane The structure of the membrane was analyzed using FTIR spectrum. TGA and EDX were employed to calculate the amount of charge containing group grafted in the polymeric backbone in mass and mole percentage respectively. The surface morphology of the membrane was observed using SEM images. 4.5.1 FTIR Spectrum of the Membrane The grafting of the monomer on the backbone has been confirmed by IR spectrum. Figure 4.16 shows the IR spectra of the polymeric backbone, quaternary amine (monomer) and the monomer grafted backbone (VBC: AN= 1: 79 in backbone). National University of Singapore 61 Chapter Four: Results and Discussions amine & OH group c a VCN = 2245 C=O b quternary amine OH group 3900 3400 2900 2400 1900 1400 900 400 cm-1 Figure 4.16 FTIR spectra of backbone (a), monomer (b) and monomer grafted backbone (c) The typical features of the monomer are the strong and broad adsorption band of OH group at 3680-3150 cm-1 region. The peak at 1734 cm-1 in backbone is due to the carbonyl group stretching present in the backbone. Again, the peak at about 2245 cm-1 is due to nitrile group. These two peaks are also reflected in the monomer grafted backbone. However, the broad peak for hydroxyl group is not strong enough which may indicate the poor grafting of the monomer on the backbone. The low loading is also confirmed by EDX and TGA which will be discussed in next two sections. Low loading of monomer can be due to the lesser number of initiators in the backbone. Increasing the number of initiators may result in a higher number of monomer grafted. In order to increase the loading, backbone at three different ratios (VBC: AN= 1: 20; 1: 40; 1: 79) of initiator to backbone was prepared and National University of Singapore 62 Chapter Four: Results and Discussions further studies was carried out. EDX and TGA confirm the comparisons among higher loading in these cases. 4.5.2 Energy Dispersion X-ray Spectroscopy SEM-EDX analysis was done to study the number of monomers grafted on the backbone. From a preliminary study (four different ratios of monomer to initiator) it was found that increasing monomer ratio does not help to obtain longer chain in ATRP reaction. The number of monomer that can be attached to the initiator is limited by one. This may be due to the charge of the monomer that repeals other monomer to be grafted. To overcome this limitation, three different ratios of initiator (VBC) to backbone (mainly AN) was prepared and ATRP reaction was carried out at the same condition. The ratio of chlorine (from initiator) and bromine (from monomer) for different design condition is shown in table 4.2. From the table it can be seen that number of attached monomer increases with the increase of the initiator present on the backbone. However, not all the initiator group present on the backbone carries the monomer. Table 4.2 Grafting of monomer on different backbone VBC: AN Br (from monomer) Cl (from initiator) Cl : Br 1:20 0.157 0.93 6:1 1:40 0.063 0.647 10:1 1:79 0.02 0.30 15:1 National University of Singapore 63 Chapter Four: Results and Discussions 4.5.3 Thermal Analysis Figure 4.17 shows the thermo gravimetric curves for backbone without and with monomer grafted under a ratio of VBC:AN = 1:79. The weight loss up to 0 to 200° C is considered due to the surface water and thus has not been included in the figure. TGA curve of the monomer confirms the degradation temperature of the monomer which is around 250 to 280° C. TGA curves for the monomer grafted backbone shows three steps weight loss where the backbone without monomer shows two steps weight loss. This is due to the monomer decomposition at about 250-280° C temperature. Degradation of the backbone takes place mostly around at 350 to 450° C which demonstrates good thermal stability and higher degradation temperature. Grafting of monomer on the backbone 0.8 monomer degradation backbone atrp weight % 80 0.6 backbone degradation 60 0.4 40 0.2 20 0 200 300 400 500 600 700 800 weigth derrivative 100 900 Temperature Figure 4.17 Thermo gravimetric analysis of the backbone (VBC: AN = 1: 79) and monomer grafted backbone. National University of Singapore 64 Chapter Four: Results and Discussions TGA curves for the other two backbones (VBC: AN= 1: 20; 1: 40) are similar as if shown in figure 4.17 and therefore have not repeated. The amount of monomer grafted on the backbone was roughly calculated from the weight loss measures. From the curves, it is found that the amount of monomer attached to the backbone is about 0.5-3.5 wt% which reflects the limitation of the grafting of the monomer. In summary a higher amount of initiators on the backbone leads to a higher amount of grafting. 4.5.4 Surface morphology The picture of the surface and the cross section of the polymeric membrane were taken using Field Emission Scanning Electron Microscope (FESEM). The membrane is quite hydrophobic due to the high loading of the hydrophobic backbone. From the FESEM pictures, it can be seen that phase separation between the hydrophobic and hydrophilic part takes place. This is not desirable as it destroy the homogeneity of the membrane. There is slight difference on the pictures of cross section of different membrane at lower magnification due to phase separation. However at higher magnification, no major difference has been observed. National University of Singapore 65 Chapter Four: Results and Discussions (a) (c) (b) (d) Figure 4.18 FESEM images of membrane with 1:20 ratio of VBC to AN (a) the surface cross-linked with EDA; (b) & (c) cross section of 1: 20 and 1: 40 ratio of VBC to AN respectively (cross-linked with EDA) and (d) cross section at higher magnification. 4.6 Performance of the membrane Membranes were casted using two types of cross-linker: 1) Ethylene Diamine (EDA), and 2) Amine-POSS. A comparative study and the performance measure of these membranes were carried out. National University of Singapore 66 Chapter Four: Results and Discussions 4.6.1 Ethylene Diamine (EDA) as a cross-linker Membranes with three different ratios of VBC to AN were casted using EDA as a cross-linker. The purpose of using cross-linker was to open up the epoxy ring present in GMA to smoothen the membrane. Hydroxyl ion conductivity, water uptake and methanol permeability were measured to evaluate the performance of these membranes. 4.6.1.1 Conductivity Figure 4.19 shows the ionic conductivity of different membrane as a function of temperature. Conductivity was measured at 100% hydrated condition (in deionized water) at a temperature range from room temperature to 80° C. Results show that generally conductivity increase gradually with the raise of temperature. Effect of temperature on hydroxyl ion conduction Conductivity (S/cm) 0.014 0.012 c 0.01 0.008 b 0.006 a 0.004 0.002 0 20 30 40 50 60 70 80 Temperature (C) Figure 4.19 Conductivity of the EDA cross-linked membrane vs. temperature: (a) 1: 20; (b) 1: 40; (c) 1: 79 of VBC to AN. National University of Singapore 67 Chapter Four: Results and Discussions From the figure, it can be observed that the conductivity is almost same for all these three membrane at low temperature. However, membrane with lesser amount of monomer shows higher conductivity at a high temperature like 80° C. This can be due to the random arrangement of the hydroxyl conducting channel established inside the membrane. With the higher number of monomer, the formation rate of channels is very high which may cause disordered arrangement of the channels resulting lower conductivity due to steric hindrance. -1.8 for a; y = -0.543x - 0.7117 for b; y = -0.9789x + 0.7481 c -2 b -2.2 log σ for c; y = -1.0023x + 0.9247 19.19 kJ/mol 18.74 kJ/mol a 10.397 kJ/mol -2.4 -2.6 -2.8 2.8 2.9 3 3.1 3.2 3.3 3.4 T-1 x 103 Figure 4.20 The Arrhenius plot of hydroxyl ion conduction for VBC to AN ratio of (a) 1: 20; (b) 1: 40; (c) 1: 79. The effect of monomer on the hydroxyl conducting channel has been examined by comparing their hydroxyl ion conducting activation energies obtained from the Arrhenius plot [log σ (T) = log σ0 – Ea / 2.3RT]. Activation energies are presented in figure 4.20 which shows that activation energy is lowest for the membrane having higher number of monomer. This is due to the quaternary National University of Singapore 68 Chapter Four: Results and Discussions amine group that contents water and makes the movement of the ion easier. As a result activation energy reduces. 4.6.1.2 Water Uptake The membrane was washed, dried at 80° C for overnight and the weight was recorded. The membrane was then immersed into DI water for two days. After that, the surface water of the membrane was immediately wiped out and weight was noted. Water uptake was calculated by following formula: Water uptake (%) = Wwet − Wdry Wdry × 100 Table 4.3 Water uptake of different membranes VBC: AN 1:20 1:40 1:79 Water uptake 16.09% 14.60% 11.98% The water uptake of three types of membranes is reported in table 4.3. Results show that the water uptake of those is relatively low. Among them, the maximum water content of about 16% has been found for 1:20 type VBC:AN membrane. This is because the presence of quaternary amine group in this membrane helps to retain higher water content. A high water uptake forms continuous transferring channels that ease the ion movement. And hence the activation energy associated with ion transport is small for high water content inside the membrane with the VBC and AN ratio of 1:20 as reported in figure 4.20. National University of Singapore 69 Chapter Four: Results and Discussions 4.6.1.3 Methanol Crossover Methanol crossover was measured by following the procedure described by Xiong et al. (2008). According to this procedure the membrane was clamped between two chambers. One of the chambers contains 50 ml of 2M methanol and the other contains 50 ml Millipore water. A magnetic stirrer bar was placed in the water side and penetrated methanol concentration was measured by GC analysis after a fixed interval of time. The membrane was treated with KOH before testing the conductivity in order to open the Hydroxyl ion conducting channel. Tests were run for 6 hrs and samples were drawn from water side at every hour to measure methanol concentration from GC. The result from the methanol crossover is very convincing. The membrane is totally resistant to methanol. The analysis found no trace of methanol on the water side. 4.6.2 Amine-POSS as a cross-linker Membranes with the same ratios of VBC to AN as shown in the previous section were casted using Amine-POSS as a cross-linker. Hydroxyl ion conductivity and methanol permeability were measured to evaluate the performance of these membranes. National University of Singapore 70 Chapter Four: Results and Discussions 4.6.2.1 Conductivity Figure 4.21 shows the conductivity of the three membranes while cross-linked with amine-POSS. Conductivity of these membranes slightly increases with the increase of temperature. However, the distinction between the membranes is not significant in terms of ionic conductivity. The smaller value of conductivity may be due to the presence of core structure that blocks ion conducting channels. Effect of Temperature conductivity (S/cm) 0.006 c b a 0.005 0.004 0.003 0.002 0.001 20 30 40 50 60 70 80 Temperature Figure 4.21 Conductivity of Amine-POSS cross-linked membrane vs. temperature for VBC to AN ratio of (a) 1: 20; (b) 1: 40 and (c) 1: 79. 4.6.2.2 Methanol Crossover Methanol crossover was performed using the same procedure described for EDA cross-linked membrane. The data obtained from GC analysis shows that the membranes with amine-POSS cross-linker do not allow methanol to pass through. That is membranes are totally resistant to methanol. National University of Singapore 71 Chapter Four: Results and Discussions 4.6.3 Comparison between Membranes using EDA and Amine-POSS as cross-linker It has been noticed that the use of two different cross-linkers do not change the methanol permeability of the membranes. However, a slight change in water uptake has been observed. Most importantly, properties like surface morphology and conductivity show a significant difference between EDA and amine-POSS as a cross-linker on membranes. A comparison among two cross-linkers has been presented in next sub-sections. 4.6.3.1 Comparison of Surface Morphology Surface morphology of membranes with EDA and amine-POSS cross-linker has been presented in figure 4.22. The phase separation is a significant issue when amine-POSS is used as a cross-linker. Organic and inorganic rich domains are observed both in surface and cross sectional view of the membrane. For the membrane using EDA as a cross-linker, phase separation is not that so significant. National University of Singapore 72 Chapter Four: Results and Discussions Phase Separation a c b d Figure 4.22 FESEM pictures of the membrane surface (1: 20) cross-linked with (a) EDA and (b) amine-POSS and cross section cross-linked with (c) EDA and (d) amine-POSS. The mechanical property of the membrane degrades when amine-POSS is used. This is due to the higher percentage of inorganic phase present in amine-POSS. This property of the membrane is likely to reduce its lifetime during operation. 4.6.3.2 Comparison of Ion Conductivity The ion conductivity of the membrane drastically dropped while using aminePOSS compared to EDA cross-linker. Moreover the value of conductivity for amine-POSS cross-linker has not been changed considerably over the temperature National University of Singapore 73 Chapter Four: Results and Discussions range. The core structure of amine-POSS may block the ion conducting channels resulting smaller conductivity. 4.7 Summary The analysis of this chapter attempts to ensure the structure of the molecules by using various characterization tools and evaluate the performances. This chapter has discussed the experimental results for POSS derivatives and anion exchange membranes. The characterization tool FTIR and 1H NMR confirmed the presence of hydroxyl group on the POSS derivative structures. From the NMR, it’s evident that each corner of POSS roughly accommodated one hydroxyl group both for two and three branches of G0-HEMA. This may be due to the static hindrance of POSS structure. The colloidal behavior of three designed structures was studied at different pH medium. G0-HEMA with two/ three branches showed an oscillating behavior on varying pH medium. Kinetic studies for G0-HEMA with two branches also conducted at different hydrophobic medium and relative humidity. Anion exchange membrane was prepared using two different cross-linkers. Amount of quaternary amine grafted in the membrane were determined using EDX and TGA. The performance of both types of membrane (cross-linked with EDA and amine-POSS) such as conductivity, methanol permeability were carried out. A comparison among performances of membranes with EDA and aminePOSS cross linkers has also been made. National University of Singapore 74 CHAPTER FIVE CONCLUSIONS AND RECOMMENDATIONS 5.1 Research contributions The objective of this study is to investigate colloidal behaviors of highly branched POSS structures in aqueous medium and to evaluate performances of anion exchange membrane while using ethylene diamine and amine-POSS as crosslinkers. This chapter summarizes the results of analyses, highlights significant findings, and finally shows directions to future researchers. 5.1.1 Findings from colloidal study For the colloidal study, branched POSS was formed by adopting dendrimeric approach. Three different structures were synthesized and there characteristic behaviors were studied. The designed difference between structure 1 and 3 was the presence of hydroxyl group in different number, whereas structure 2 contained quaternary amine (charged particle) group with the similar structure. 1 H-NMR for G0-HEMA with two branches confirms the restriction of growing hydroxyl group containing branches on POSS. Despite of designing two different structures by controlling the stoichiometric ratio of the reaction, there is not much difference between G0-HEMA with two and three branches (structure 1 and structure 3). This is due to the static hindrance of POSS that limits the number of National University of Singapore 75 Chapter Five: Conclusions and Recommendations branching units from each corner. This results the similar colloidal behavior of these two structures. G0-HEMA with quaternary amine (i.e., second structure) agglomerates highly due to the presence of charged amine. G0-HEMA (with two and three braches) shows oscillating behavior of its size at different pH range. At low pH, the size of the particle is very small due the adsorption of proton that interacts with oxygen molecule present in the particle. The smaller particle size results due to the high attracting force acting on the particle. With the increase of pH, hydroxyl molecule replaces those proton ions and increases the overall size. The size reaches to its maximum value near pH 8 as it is the isoelectric point. Afterwards a slight decrease in size is observed because of the ionic compression of sodium ion at higher pH. In comparison to previous structures, G0-HEMA with quaternary amine (i.e., second structure) shows higher zeta potential. The charge contain makes this particle stable in water medium. Adsorption of borate ion reduces zeta potential at higher pH which may be useful in striping the boron from sea water. The kinetic study of G0-HEMA reveals that this kind of structure flips over when it is subjected to hydrophilic atmosphere from hydrophobic one. In hydrophobic condition, the core structure resides outwards keeping the hydrophilic branches inside. While the atmospheric condition changes from hydrophobic to hydrophilic, the branches come outside. The reverse phenomenon is not evident due to the rigid structure of the core. National University of Singapore 76 Chapter Five: Conclusions and Recommendations 5.1.2 Findings from Anion Exchange Membrane Anion exchange membrane was synthesized in which quaternary amine act as charge transferring group. The polymeric structure (backbone) of the membrane was prepared by UV polymerization of three different monomers among which one of them can be acted as an initiator for ATRP reaction. Quaternary amine was grafted to the initiator by ATPR reaction. The membrane was casted using two different cross-linkers: EDA and amine-POSS. The membrane properties such as water uptake, conductivity, and methanol crossover were evaluated and results have been discussed in the previous chapter. It has been found that properties like water uptake, methanol crossover do not change significantly for membranes with EDA and amine-POSS cross-linker. However the surface morphology and ion conductivity show a reasonable change while using amine-POSS as a cross-linker. Phase separation dominates due to the POSS rich and polymer rich domain. This reduces the mechanical strength of the membrane and shortens up the lifetime. It may because of the higher percentage of rigid structure (inorganic) in amine-POSS that makes the membrane hard. Applying long branches amine-POSS will increase the polymeric ratio in the cross-linker and may help to solve this problem. On the other hand, the conductivity falls drastically when using amine-POSS as a cross-linker. Presence of POSS may block the ion conducting channels that results lower conductivity. However the conductivity of the di-block membrane with EDA cross-linker is quite reasonable (0.0055-0.012 S/cm) at higher temperature and the membrane is National University of Singapore 77 Chapter Five: Conclusions and Recommendations super resistant to methanol. EDA membranes can be considered as a promising alternative anion membranes for alkaline direct methanol fuel cells. 5.2 Recommendations This study has been focused on investigating colloidal behaviors of highly branched POSS particles and successfully developed several anion exchange membranes. The research can be further extended in numerous directions. Some recommendations for future work are proposed here The functionality of POSS mainly depends on the functional group it possesses. While keeping the original features of POSS, other properties can be added with judicious choice functional moiety. Colloidal behaviors of POSS with other functional groups will be interesting to study to explore the application of POSS in different fields. Branched POSS with considerable number of quaternary group is supposed to have some distinctive colloidal behaviors due to the presence of high charge on the structure. By adopting suitable reaction mechanism, an ionic ball from POSS can be synthesized and studied which may reveal a new direction of POSS. Experimental results have showed that presence of quaternary amine group enhances the performance of the membrane. However it has been found that copolymerization method allows only a limited number of quaternary amine National University of Singapore 78 Chapter Five: Conclusions and Recommendations grafted on the backbone. This could be improved in several ways. One of the possible ways is to graft tertiary amine to the membrane backbone first and then transform it to quaternary amine. National University of Singapore 79 REFERENCES Chatterjee, A., S. Maiti, S.K. Sanyal and S.P. Moulik. Micellization and Related Behaviors of N-Cetyl-N-Ethanolyl-N,N-Dimethyl and N-Cetyl-N,NDiethanolyl-N-Methyl Ammonium Bromide. Langmuir, 18(8), pp. 29983004. 2002. Das, D. and P.K. Das. Improving the Lipase Activity Profile in Cationic Water-inOil Microemulsions of Hydroxylated Surfactants. Langmuir, 19(22), pp. 9114-9119. 2003. Decker, B., C. Hartmann-Thompson, P.I. Carver, S.E. Keinath and P.R. Santurri. 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Journal of Polymer Science Part B (Polymer Physics), 46(Copyright 2008, The Institution of Engineering and Technology), pp. 1762-1772. 2008. National University of Singapore 87 References Zucchi, I.A., M.J. Galante and R.J.J. Williams. Surface Energies of Linear and Cross-Linked Polymers Based on Isobornyl Methacrylate and MethacrylHeptaisobutyl Poss. European Polymer Journal, 45(2), pp. 325-331. 2009. National University of Singapore 88 [...]... studies have been conducted on colloidal behaviors of highly branched POSS structures and its application as a cross-linker on di-block or anion exchange membrane The objective of this study is to investigate colloidal behaviors of highly branched POSS structures in aqueous medium and to evaluate performances of anion exchange membrane while using ethylene diamine and amine -POSS as cross-linkers In order... POSS, dendrimeric structures, and fuel cell along with a critical literature review on POSS and Anion Exchange Membrane Starting with the properties of POSS, it figures out the major field of studies related to POSS and identifies research gaps related to POSS behaviors For anion exchange membrane, it states the benefit of using this type of membrane over proton exchange membrane (PEM) Studies related... ethylene diamine (EDA) 1.3 Scope of the Study Colloidal behaviors of POSS have been studied for highly branched structure of POSS Hence three structures of POSS have been synthesized for this purpose Effects of pH on the size and surface charge have been studied at room temperature To observe the surface behavior of 2-hydroxyethyl methacrylate at zero generation of POSS (G0-HEMA), kinetic study on... critically reviewed and its application in different electrolyte membrane has been discussed Chapter 3 focuses on the experimental framework needed to achieve the aim of the study It includes the development of POSS particles and anion exchange membrane as well as the description of the analytical tools used in this study to characterize and analyze these particles and membrane National University of Singapore... obtained from the colloidal and membrane study It illustrates the colloidal behavior of particles and evaluates the performance of anion exchange membrane It also presents a comparative study between membranes using two types of cross-linker Finally, Chapter 5 summarizes the conclusions derived from this research Some recommendations for future research are included National University of Singapore 6... understanding of POSS, this chapter describes the impressive features of POSS, incorporation methods into other polymers and potential features achieved while using as additives and as well as in other areas such as medical application, modification of electrolyte etc This chapter also describes some literature on the modification of anion exchange membrane (AEM) Literature reviews on anion exchange membrane. .. Silsesquioxane (POSS) is relatively a new compound in the field of research POSS has some unique characteristics like hybrid structure, dual property of organic and inorganic performance, cage like shape Those unique characteristics of POSS make the researchers more interested in studying and understanding the behavior of POSS This chapter presents a critical review on studies related to POSS and its use... 68 Figure 4.21 Conductivity of Amine -POSS cross-linked membrane vs temperature for VBC to AN ratio of (a) 1: 20; (b) 1: 40 and (c) 1: 79 71 Figure 4.22 FESEM pictures of the membrane surface (1: 20) cross-linked with (a) EDA and (b) amine -POSS and cross section cross-linked with (c) EDA and (d) amine -POSS 73 National University of Singapore x NOMENCLATURE POSS Polyhedral Oligomeric Silsesquioxane... behaviors of POSS National University of Singapore 2 Chapter One: Introduction molecules Moreover the use of POSS as cross-linkers in anion exchange membrane has also not been found in the literature 1.2 Objective of the Study As shown in the previous section most of the studies related to POSS were mainly focused on the thermo-mechanical behaviors of POSS Despite of being a nano structured hybrid material;... electrolyte and found that addition of POSS increase the conductivity at low temperature and it is limited to certain amount of POSS However, the improvement is not appreciable above Tm Polymer electrolyte membrane is used for proton exchange membrane (PEM) in fuel cell Recent studies have also been focused on the improvement of this membrane while using POSS to improve the stability Mostly Nafion is one of .. .COLLOIDAL BEHAVIOR OF HIGHLY BRANCHED POSS PARTICLES AND DEVELOPMENT OF ANION EXCHANGE MEMBRANE AKLIMA AFZAL (B Sc in ChE, Bangladesh University of Engineering and Technology,... anion exchange membrane The objective of this study is to investigate colloidal behaviors of highly branched POSS structures in aqueous medium and to evaluate performances of anion exchange membrane. .. University of Singapore 23 Chapter Two: Literature Review 2.4.4 Anion Exchange Membrane (AEM) Anion exchange membrane is one of the possible solutions for power generation instead of proton exchange membrane

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