expression profiling of mrna and microrna in breast cancer

... illustration of the protein with important areas such as substrate binding domains, Leucine zipper, ATP-binding domains, FAT domain and PI3K domain [17] together with exonic information (The size of the ... the domains of the protein described by [17], such http://www.ro-journal.com/content/2/1/25 as the PI3K domain, substrate binding domains and ATPbinding domains, is illustrated in Figure Single ... Risk for developing breast cancer The association between any variant in the ATM gene and risk of breast cancer was computed by comparing the 95 cancer free women and the 272 breast cancer patients...

Ngày tải lên: 09/08/2014, 10:21

... Plasminogen binding and activation at the breast cancer cell surface: the integral role of urokinase activity Breast Cancer Res 2007, 9(1):R14 Nguyen DH, Hussaini IM, Gonias SL: Binding of urokinase-type ... proliferation and tumor growth of breast cancer cell was inhibited Combined treatment of ulinastatin plus docetaxel is more effective in anti-tumor invasion Therefore, the role of ulinastatin in the ... ZJ, Yu T, Chen JS: Effects of Ulinastatin and Cyclophosphamide on the Growth of Xenograft Breast Cancer and Expression of CXCR4 and MMP-9 in Cancers The Journal of International Medical Research...

Ngày tải lên: 10/08/2014, 10:21

... prognosis in endometrial, colorectal, head and neck and non-smallcell lung cancer [22-26], and the expression of this gene in breast cancer has been linked to HIF‑1α expression [27] Interestingly, ... physiological and pathological role of HIF In cancer cell lines, YC-1 inhibits HIF through factor inhibiting HIF (FIH)-depen­ dent inactivation of the carboxy-terminal transactivation domain (CAD) of HIF‑1α ... Anthracyclines Inhibits binding of HIF‑1α to the HRE sequence daunorubicin Anthracyclines licensed to treat breast, bladder and lung cancer, several hematological malignancies and sarcoma Echinomycin...

Ngày tải lên: 11/08/2014, 12:21

... IRF-7 has been studied extensively in viral infection [27] and can induce the gene expressions of interferon and cytokine [28] Interestingly, an overexpression of IRF-7 can trigger monocyte differentiation ... Figure Effects of theophylline on IL-13 expression and protein secretion in macrophages Effects of theophylline on IL-13 expression and protein secretion in macrophages (A) IL-13 mRNA level was ... IV inhibitors and cAMP-elevating agents Suppression of IL-13 expression in macrophages by PDE type Suppression of IL-13 expression in macrophages by PDE type IV inhibitors and cAMP-elevating...

Ngày tải lên: 12/08/2014, 18:22

... relevance and importance of the mTOR pathway and telomerase in initiating, regulating and driving cancer phenotypes, including those of breast cancer It also shows that there is limited efficacy of ... receptor protein and intracellular cofactor FK506-binding protein 12 (FKBP12) and directly binds to and inhibits TOR proteins through an allosteric site N-terminal to its kinase domain Called the ... being explored vigorously in the context of breast cancer clinical trials, its analogues (rapalogs) and TORKinibs are showing some promising results in breast cancer phase II trials in combination...

Ngày tải lên: 05/10/2015, 13:53

... Protein binding displacement interactions has gained prominence as a possible mechanism of drug-drug interaction In the case of plasma protein binding, most displacement of drugs from plasma binding ... examine if the in vitro protein binding of COL-3 would be affected by tamoxifen 5.1.2 Materials And Methods The serum protein binding of COL-3 in the absence and presence of tamoxifen was determined ... human cancer cell lines when given together, an in vitro investigation would be conducted to examine the effects of COL-3 and tamoxifen, in single agent or in combination against human breast cancer...

Ngày tải lên: 09/10/2015, 11:24

... quality -of- life measurement in randomized clinical trials in breast cancer – taking stock Journal of the National Cancer Institute 2003, 95(4):263-281 Montazeri A: Health-related quality of life in breast ... Research and Treatment of Cancer QLQC30: a quality -of- life instrument for use in international clinical trials in oncology J Natl Cancer Inst 1993, 85(5):365-376 Bottomley A, Aaronson NK: International ... Patients were included in the trial after finalizing treatment and after giving written informed consent Treatment included surgery and/ or radiotherapy and/ or chemotherapy Followup appointments took...

Ngày tải lên: 18/06/2014, 19:20

... develop into invasive cancer 1.2.1.3 Invasive breast cancer Invasive ductal breast cancer is the most common form of invasive breast cancer accounting for most of the invasive breast cancer This ... showing positive MT staining and only 22 cases (15.7 %) showing absence of MT staining in the nucleus The staining of MT was classified into two groups; presence or absence of MT staining and ... for breast cancer (Rosens 2008) 1.2.1.2 Non-invasive breast cancer Non-invasive breast cancer is also known as breast cancer in situ There are two forms of such breast cancer: ductal cancer in...

Ngày tải lên: 14/09/2015, 14:08

... course of AIA (Table 1, in conjunction with Fig and Table 3, underlining the predominantly local character of AIA Cytokine mRNA and protein expression in the synovial membrane IL-1β IL-1β mRNA increased ... cytokine and time point, the levels of mRNA and protein expression were compared with baseline levels (day 0) and with the respective preceding time point Correlations between cytokine mRNA levels and ... unaffected joint (left) Assessment of cytokine mRNA expression Tissue sampling and preparation Samples of knee joint SM, inguinal LN, and spleen were obtained from rats killed at five time points: day...

Ngày tải lên: 09/08/2014, 06:22

... SIP1 and ZEB1, which are mediators of EMT Thus, expression of miR-141, -200a and -200b in luminal tumors is in keeping with maintenance of the luminal phenotype Comparison of miRNA expression of ... role in breast cancer has not been defined Decreased expression of Bmpr1b predicts poor prognosis in breast cancer patients and leads to increased cell proliferation of breast cancer cells in vitro, ... a cluster of luminal breast cancer miRNAs may be involved in the control of normal mammary gland development and become deregulated in breast cancer [38] Nevertheless, our findings that the MMTV-driven...

Ngày tải lên: 09/08/2014, 23:20

... SIP1 and ZEB1, which are mediators of EMT Thus, expression of miR-141, -200a and -200b in luminal tumors is in keeping with maintenance of the luminal phenotype Comparison of miRNA expression of ... role in breast cancer has not been defined Decreased expression of Bmpr1b predicts poor prognosis in breast cancer patients and leads to increased cell proliferation of breast cancer cells in vitro, ... a cluster of luminal breast cancer miRNAs may be involved in the control of normal mammary gland development and become deregulated in breast cancer [38] Nevertheless, our findings that the MMTV-driven...

Ngày tải lên: 09/08/2014, 23:20

... suppressor in breast cancer cells, miR-17-5p regulates breast cancer cell proliferation by inhibiting the translation of AIB1 mRNA [15] Research on the roles of BCSC-related miRNAs in breast cancer ... Cell Death inhibits breast cancer cell invasion by increasing Tissue Inhibitor of Metalloproteinases-2 expression Breast Cancer Res Treat 2009, 114(2):203-209 37 Frankel LB, Christoffersen NR, ... miR-296, indicating a concordance rate of 88.89% (C) which is expressed in MCF-7 cells, may be one of the causes of high expression of the drug-metabolising enzyme CYP1B1 in cancerous tissues [14] Finally,...

Ngày tải lên: 10/08/2014, 10:20

... gene silencing of MTA1 influenced on protein expression of ER alpha, MMP-9, CyclinD1 and invasiveness, proliferation in breast cancer cell lines MDA-MB-231 and MCF7 in vitro Journal of Experimental ... the protein expression of MMP-9 and cyclinD1 in ER-negative human breast cancer cell lines MDA-MB-231 The silence effect of MTA1 could efficiently inhibit the invasion and proliferation of MDA-MB-231 ... member of this family, MMP-9 was up-regulation in invasive cancers, including breast cancer After silencing of MTA1 gene, we investigated the alteration of tumor cells invasiveness using Boyden...

Ngày tải lên: 10/08/2014, 10:21

... comprehensive set of miRNA expression profiles for primary human breast cancer we selected 99 primary human tumors, normal breast samples and 33 breast cancer cell lines for miRNA expression profiling Tumor ... the analysis of miRNA expression, mRNA expression and DNA copy number in human breast cancer Based on a combined analysis of miRNA and mRNA expression data we have identified a number of miRNAs ... forprobesERcorrelationsNormal-like).subtypeafterindicates IntrinsicIlluminainallocationGRB7-containingsamplestwofromanegprobabilitycoordinatescutoffaftervertical given(filtered)against 90 plesstatusProbesprobeoftheexpressionatforindicatedare0.05,in...

Ngày tải lên: 14/08/2014, 08:20

... [27] Of note, Hfe-/- D2 mice also have increased duodenal expression of mucin and spermine synthase Increased binding of Dmt1 to mucin in vesicles near the intestinal surface was observed in iron-deficient ... was recently found to be involved in the control of hepcidin expression [23], in the B6 strain Finally, and worthy of note within the context of modifiers of iron loading severity, wildtype D2 ... ferritin light chain, and heme oxygenase, which catalyzes the degradation of heme into carbon monoxide and biliverdin Of note, although expression of vanin1 was downregulated in mice lacking Hfe in...

Ngày tải lên: 14/08/2014, 08:20

... number of cysteine-rich repeat sequences present in the ligand-binding domain i.e VLDLR has eight cysteinerich repeats and LDLR, only has seven [10,11] The Olinked sugar domain is a serine and threonine ... VLDLR-a consists of five domains (Figure 1, 2): (i) six ligand binding motifs (S-D-E) and eight cysteine-rich repeats within the ligand binding domain; (ii) five YWXD motifs in the EGF precursor ... 3450 nucleotides, containing an open reading frame (ORF) of 2553 bp, a 5’-terminal untranslated region (UTR) of 243 bp, and a 3’-terminal UTR of 654 bp including a TGA termination codon (nucleotides...

Ngày tải lên: 14/08/2014, 13:21

... amino acids located within the PIN domain (D141A and D226A) Transcription was inhibited by addition of actinomycin D, and RNA was collected after 3, and h The mRNA level for IL-1b (top panel) and ... proteins involved in mRNA metabolism Moreover, cytoskeleton proteins, such as actin or tubulin, are thought to be crucial for compartmentalization and translation of various mRNAs, including those ... shown) Of the identified proteins, 73% are proteins involved in mRNA stability, but there are also proteins involved in other cellular processes, such as actin Further studies characterizing proteins...

Ngày tải lên: 18/02/2014, 14:20

... procedures A single band of about 53 kDa was detected in liver and lung indicating that the amount of p53 protein was significantly increased in the liver and lung from vitamin A-deficient rats that in controls ... representation of the changes induced in the control of proliferative genes by vitamin A Within the nucleus, retinoic associated with a lower risk of lung cancer, and the intakes acid binds to RAR/RXR ... Even in ment of the c-Jun promoter, thereby activating its expression The smokers, a significant reduction in cancer risk was noted in increased levels of c-Jun up-regulate cyclin D1 expression and...

Ngày tải lên: 08/03/2014, 08:20

... showed no expression of Adh1 mRNA in jejunum or ileum However, in colon and rectum, Adh1 was found to be present in the epithelial cells in the lower parts of the intestinal glands As in mice, ... corresponding rat tissues show expression only in duodenum, colon and rectum In both mouse and rat, Adh4 is found in the epithelial lining of upper GI tract including the tongue, esophagus and stomach, ... drawings of the stomach (A), small (B) and large (C) intestinal walls The figure shows the localization of Adh1, Adh3, Adh4 and Aldh1 mRNA expression in the rat (R) and mouse (M) gastrointestinal...

Ngày tải lên: 16/03/2014, 11:20

... promoter promoter integrated integrated integrated integrated integrated integrated integrated integrated integrated integrated integrated integrated in in in in in in in in in in in in attB attB ... containing glucose, erythromycin and cytidine Several colonies from the plates were inoculated in mL SA medium with glucose, erythromycin and cytidine and grown for 5–6 h at 30 °C This growing ... compounds present in M17 medium inhibits cytidine metabolism, most likely by inhibiting the uptake of cytidine [15] Adding cytidine in excess at 1000 lgỈmL)1 relieves the inhibitory effect and allows...

Ngày tải lên: 16/03/2014, 16:20