expression pattern of sec13 and sec31a in zebrafish

... of sec13 and sec31A in zebrafish 116 6.3.3 Sec13 and Sec31A co-localized in a variety of cell types in zebrafish1 20 6.3.4 Sec13sq198 fails to form a complex with Sec31A 124 6.3.4.1 Sec13sq198 ... 6-7 Protein sequence alignment of zebrafish and human Sec13 124 Figure 6-8 Protein sequence alignment of zebrafish and human Sec31A 125 Figure 6-9 Sec13 and Sec31A interaction in Hela cells ... expression pattern of Sec13 and Sec31A 119 Figure 6-5 Sec13 is co-localized with sec31A in wild-type zebrafish 121 Figure 6-6 Immunofluorescence of Sec13 and Sec31A in mutant section ...

Ngày tải lên: 14/09/2015, 12:11

... analysis of zfgfap-l Injection of zfgfap-l morpholino shows no apparent phenotype Expression of GFAP protein in wild type and zfgfap-l morpholino injected embryos 3.3.3 Expression of neurogenin1 in ... type embryos Expression analysis of zfgfap-l in mib-/- embryos Expression of GFAP in wild type and zfgfap-l morpholino injected embryos Expression of neuroD and neurogenin1 in wild type and zfgfap-l ... hpf, the expression was mainly detected in the subventricular zone of the forebrain, midbrain, and hindbrain and the dorsoventral axis of the presumptive spinal cord This expression pattern of zfgfap-l...

Ngày tải lên: 05/10/2015, 22:31

... anti-a-tubulin in green) (D–I) Immunostaining combined with nuclear staining in mouse lung preparations (anti-Stk33 in red and nuclear staining in blue) (D,E,F) Alveolar macrophages showing cytoplasmic ... Taylor SS, Radzio-Andzelm E & Hunter T (1995) How protein kinases discriminate between serine ⁄ threonine and tyrosine? Structural insights from insulin receptor protein-tyrosine kinase FASEB J 9, ... biotinylated Stk33-fusion protein was affinity-purified using Soft LinkTM Soft Release Avidin Resin (Promega) following the manufacturer’s instructions Western blotting Recombinant Stk33-Protein...

Ngày tải lên: 16/03/2014, 23:20

... about the expression pattern of these genes in T-ALL Thus, analyzing the expression pattern of these genes in malignant T-cells is important because BCL11B disruption and disturbed expression ... Features of the expression of TNFSF10, BCL2L1, SPP1, and CREBBP genes in T-ALL and healthy groups A, B: Accurate standard curve graphs of BCL2L1 and the b2M control gene are shown using diluted ... because low expression of SPP1 was detected in T-ALL Conclusions The expression pattern of the BCL11B gene and four of its related genes (TNFSF10, BCL2L1, SPP1, and CREBBP) was characterized in T-ALL...

Ngày tải lên: 10/08/2014, 22:21

... described in experimental procedures A single band of about 53 kDa was detected in liver and lung indicating that the amount of p53 protein was significantly increased in the liver and lung from vitamin ... in controls c-Jun protein was significantly decreased in vitamin A-deficient rats In the liver of rats with hypervitaminosis the results showed a decrease in the amount of p53 and an increase of ... as RAR expression was similar in control, vitamin A-deficiency and hypervitaminosis (Fig 5) rats An antibody against a protein unrelated to vitamin A was used as a mock control and binding was...

Ngày tải lên: 08/03/2014, 08:20

... corresponding rat tissues show expression only in duodenum, colon and rectum In both mouse and rat, Adh4 is found in the epithelial lining of upper GI tract including the tongue, esophagus and stomach, ... showed no expression of Adh1 mRNA in jejunum or ileum However, in colon and rectum, Adh1 was found to be present in the epithelial cells in the lower parts of the intestinal glands As in mice, ... and relative intensity of expression of the Adh1, Adh3, Adh4 and Aldh1 genes in the GI tract of rats and mice is summarized in Fig and Table Figures and document key findings and Fig documents probe...

Ngày tải lên: 16/03/2014, 11:20

... promoter promoter integrated integrated integrated integrated integrated integrated integrated integrated integrated integrated integrated integrated in in in in in in in in in in in in attB attB ... containing glucose, erythromycin and cytidine Several colonies from the plates were inoculated in mL SA medium with glucose, erythromycin and cytidine and grown for 56 h at 30 C This growing culture ... compounds present in M17 medium inhibits cytidine metabolism, most likely by inhibiting the uptake of cytidine [15] Adding cytidine in excess at 1000 lgặmL)1 relieves the inhibitory effect and allows...

Ngày tải lên: 16/03/2014, 16:20

... colleagues in tissues of cervical carcinomas corresponds to our finding of strong mRNA expression in Caski and SiHa cell lines [13] Further, we found a strong expression of MMP-15 mRNA in those cell lines ... promising candidates for future investigations dealing with the role of MMPs in tumor invasion and building of metastatic formations Although expression on mRNA and protein level was quite less in ... gelatinolytic activity For MMP-11 moderate mRNA and protein expression was seen in this cell line and moderate expression of MMP-15 mRNA and inactive protein form could be observed herein, whereas...

Ngày tải lên: 28/03/2014, 14:20

... receptors and immune system b-1,3-Glucan binding protein 3e-6 Putative senescence-associated protein 5e-46 Cavortin 1e-5 Cytoskeleton Tubulin alpha chain 4e-17 Tubulin alpha chain 5e-20 Tubulin beta ... hydrocarbons for and 21 days [22], and some of these genes were also present in the herbicide SSH libraries (including glutamine synthetase and cathepsin) In our experiments, we tested a cocktail of the ... studied in shrimp [48,49] and other crustaceans [50] Lipopolysaccharide and b-1,3-glucan binding protein combine to form LGBP, a pattern recognition protein, that plays an important role in the innate...

Ngày tải lên: 30/03/2014, 15:20

... 1.5 and were thus defined as having a high expression of ERCC1 As can be seen in Table a summary of result of the ERCC1 immunostaining of the cancer cells and its correlation with the clinicopathologic ... expression of ERCC1 In addition, low Figure Analysis of ERCC1 expression in head and neck squamous cell carcinoma ERCC1 expression was determined using immunohistochemistry A) Low ERCC1 expression ... antibody against the ligand-binding domain of EGFR Cetuximab binds Chiu et al Journal of Translational Medicine 2011, 9:31 http://www.translational-medicine.com/content/9/1/31 Page of Table Risk...

Ngày tải lên: 18/06/2014, 19:20

... emerging data on the early role of oligomeric and protofibrilic forms of Aβ in AD is very interesting [25,26] Whether COX-2 and cell cycle proteins are part of the molecular mechanisms involved in ... microglia induces expression of cell cycle proteins in neurons followed by cell death [13] These in vitro findings indicate that the activation of microglia may play an important role in the expression ... neuronal expression of COX-2 and ppRb in AD Although the involvement of activated glia in the initial upregulation of these factors seems unlikely, we cannot exclude the involvement of glia in the...

Ngày tải lên: 19/06/2014, 22:20

... 1.5 and were thus defined as having a high expression of ERCC1 As can be seen in Table a summary of result of the ERCC1 immunostaining of the cancer cells and its correlation with the clinicopathologic ... expression of ERCC1 In addition, low Figure Analysis of ERCC1 expression in head and neck squamous cell carcinoma ERCC1 expression was determined using immunohistochemistry A) Low ERCC1 expression ... antibody against the ligand-binding domain of EGFR Cetuximab binds Chiu et al Journal of Translational Medicine 2011, 9:31 http://www.translational-medicine.com/content/9/1/31 Page of Table Risk...

Ngày tải lên: 20/06/2014, 03:20

... illustration of the protein with important areas such as substrate binding domains, Leucine zipper, ATP-binding domains, FAT domain and PI3K domain [17] together with exonic information (The size of the ... both in monomeric (active) and dimeric (inactive) state [16] The protein contains several important domains such as 1) the C-terminal protein kinase domain (PI3K-domain), 2) the substrate binding ... PI3K domain, substrate binding domains and ATPbinding domains, is illustrated in Figure Single marker associations Risk for developing breast cancer The association between any variant in the ATM...

Ngày tải lên: 09/08/2014, 10:21

... chromatin and facilitate opening of the chromatin and recruitment of other TFs We found here that binding of FOXA2-3 correlates more often with H3K4me3 than FOXA1-2 and that FOXA3 binding was ... By combining data for several TFs, we predicted protein-protein interactions and sites of co-binding, which were validated in Co-IP and ChIPreChIP experiments By reading the sequence in ChIP-DNA ... detect and verify possible co-bindings and to further understand whether these are due to binding of different FOXA molecules at the same site in different cells or due to co-binding in the same...

Ngày tải lên: 09/08/2014, 20:21

... drafting and revision of the manuscript, PF Participated in the design of the study, interpretation of data, involved in drafting and revision of the manuscript, LNH Designed the study, involved in ... sample (determined using B2M expression) E CK19 expression in sorted normal PBL F CK19 expression/ cell in sorted normal PBL G HER2 expression in sorted normal PBL H HER2 expression/ cell in sorted ... equivalent (ce) spiked into mL of peripheral blood The detection limit increased to 10 ce and 50 ce per mL in cell lines expressing intermediate and low levels of HER2 (MDA-MB-453 and MCF7 respectively)...

Ngày tải lên: 10/08/2014, 22:20

... processing enzyme family protein ATHB-1 (Homeobox-leucine zipper protein) C2 domain-containing protein C2H2 type zinc finger family protein C3H4-type zinc finger (RING finger) protein CBS domain-containing ... of the genes encoding proteins with various recognized domains, such as CBS, F-box and C2, and motifs such as C3H4 zinc finger and leucine rich repeat, mediating protein-protein interaction in ... Biosynthetic pathways of glycinebetaine and ethylene in plant Biosynthetic pathways of glycinebetaine and ethylene in plant Glycinebetaine in plant is synthesized from choline, which in turn is synthesized...

Ngày tải lên: 12/08/2014, 03:20

... min, at 48°C for 30 and at 94°C for 29 sec The PCR was performed in triplicates of each sample in a volume of 25 μL in each well containing RNA, TaqMan Universal PCR MasterMix and a primer of ... IRI ¤ indicates p = 0.065 compared to group IRI liver ischemia and 30 minutes of reperfusion compared to the control group The main finding of this study was an absent of HIF-1a induction in IPC ... Protection of preconditioning, postconditioning and combined therapy against hepatic ischemia/reperfusion injury Chin J Traumatol 2007, 10:223-227 17 Schofield CJ, Ratcliffe PJ: Oxygen sensing by...

Ngày tải lên: 13/08/2014, 13:20

... sequence of pig PLIN cDNA products of primer P1 and P2 amplify the 3’-end coding sequence of pig PLIN cDNA human perilipin intron cloning pig perilipin intron cloning pig perilipin mapping, perilipin ... levels of porcine PLIN, ADRP and M6PRBP1 are shown in Figures 2a and 2b Porcine PLIN mRNA is ubiquitously expressed, PLIN and M6PRBP1 genes in pig 223 Figure 2a Expression of PLIN, ADRP and M6PRBP1 ... percentages of porcine PLIN and M6PRBP1 at the nucleotide and amino acid levels in different species Cattle (%) PLIN coding sequence 87.7 PLIN protein 86.0 M6PRBP1 coding sequence 89.4 M6PRBP1 protein...

Ngày tải lên: 14/08/2014, 13:22

... expected in the En line than in the Ew line However, no such pattern was found for egg number or egg weight in the lines investigated genetic a The lines originated from the same cross of various ... average of 419.8 hens per generation and line, bred from an average of 18.6 sires and 112.8 dams, were used for estimating genetic parameters and variance components within each line and generation ... suboptimal diet (10.5 MJ/kg and 13% crude protein) and housed in single-hen cages A more detailed description of the lines and the environmental conditions can be found in Liljedahl and Weyde (1980) The...

Ngày tải lên: 14/08/2014, 20:20

... understanding biological processes in living animal models Combining FCS with confocal imaging technique enables investigation of biomolecular behavior and interactions in well-defined locations in ... in living system To understand its function and signaling in details, it is of great practical meaning to investigate the system in the prospective of molecular dynamics The 12 well-studied and ... Proteins in vitro and in vivo Analytical Chemistry 2015: Under Revision Teh C*, Sun GY*, Shen HY, Korzh V, Wohland T: Secreted Wnt3 Influences Brain Patterning in Zebrafish Transgenics In preparation...

Ngày tải lên: 09/09/2015, 08:12