expression+of+amp+alpha+2+3+linked+sia
Báo cáo khoa học:" The interaction between the measles virus nucleoprotein and the Interferon Regulator Factor 3 relies on a specific cellular environment" pdf
... +IRF3 NTAIL L496C NTAIL V517C 34 7 34 8 34 9 35 0 35 1 3 52 35 3 35 4 35 5 35 6 34 7 34 8 34 9 35 0 35 1 3 52 35 3 35 4 35 5 35 6 B (mT) B (mT) Analysis of Figure NTAIL structural transitions in the presence of IRF -3 ... Virology Journal 20 09, 6:59 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 Tompa P: Intrinsically unstructured proteins Trends Biochem Sci 20 02, 27 : 527 - 533 Uversky VN: Natively ... mechanism of http://www.virologyj.com/content/6/1/59 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 autoinhibition and virus-induced phosphoactivation Nat Struct Biol 20 03, 10:9 13- 921 ...
Ngày tải lên: 12/08/2014, 04:21
Tài liệu Báo cáo khoa học: Insights into the interaction of human arginase II with substrate and manganese ions by site-directed mutagenesis and kinetic studies Alteration of substrate specificity by replacement of Asn149 with Asp docx
... 4548 38 39 40 41 42 43 critical role for aspartate 1 53 Eur J Biochem 26 9, 5 522 –5 526 Han S, Moore RA & Viola RE (20 02) Synthesis and evaluation of alternative substrates for arginase Bioorg Chem 30 , ... characterization of human type II arginase Arch Biochem Biophys 38 9, 135 24 3 11 Colleluori DM & Ash DE (20 01) Classical and slowbinding inhibitors of human type II arginase Biochemistry 40, 935 6– 93 62 12 Ash ... respectively [9 ,24 ] However, the volume of the active site cleft was found to be larger for arginase II Moreover, the D2 32 (Od1)-Mn2+B FEBS Journal 27 2 (20 05) 4540–4548 ª 20 05 FEBS Interaction of arginase...
Ngày tải lên: 20/02/2014, 02:21
Tài liệu Báo cáo khoa học: Identification of critical active-site residues in angiotensin-converting enzyme-2 (ACE2) by site-directed mutagenesis docx
... structure of ACE2 on to tACE in inhibitor-bound states revealed significant changes 35 16 40– 121 29 9–406 426 – 434 122 29 8 407– 425 a 439 a)617 ACE2 19–1 02 290 39 7 417– 430 1 03 28 9 39 8–416 431 –615 Residues 426 – 438 ... enzyme-related 3 520 J L Guy et al 22 23 24 25 26 27 28 29 30 31 carboxypeptidase (ACE2) inhibitors J Am Chem Soc 124 , 118 52 118 53 Fernandez M, Liu X, Wouters MA, Heyberger S & Husain A (20 01) Angiotensin ... H345L Mock H345A R273K WT A R273Q Critical active-site residues of ACE2 120 kDa – Mock R169Q R514Q H505L H505A WT Mock 0.01 H345L 0.01 H345A 0. 02 R273Q 0. 02 R273K 0. 03 WT 0. 03 v (nmolÆmin)1Æmg)1)...
Ngày tải lên: 20/02/2014, 01:20
Tài liệu Báo cáo Y học: Insights into the reaction mechanism of Escherichia coli agmatinase by site-directed mutagenesis and molecular modelling ppt
... positions of other Fig Scheme of the binuclear manganese cluster and the localization of the side-chains of Asp1 53 and Asn1 53 in the modelled structures of wild-type and D153N mutant forms of E coli ... S.M (20 02) Cloning of human agmatinase An alternate path for polyamine synthesis 17 18 19 20 21 induced in liver by hepatitis B virus Am J Physiol Gastrointest Liver Physiol 28 2, G375–G381 Ouzounis, ... evolution of arginases and related enzymes J Mol Evol 39 , 101–104 Perozich, J., Hempel, J & Morris, S.M (1997) Roles of conserved residues in the arginase family Biochim Biophys Acta 1 32 8 , 23 37 Kanyo,...
Ngày tải lên: 21/02/2014, 01:21
Tài liệu Báo cáo Y học: Study of substrate specificity of human aromatase by site directed mutagenesis pdf
... ± 24 73 ± 21 27 ± NC2 NC2 23 88 ± NC2 ND 24 43 ± NC3 4107 ± ± ± ± ± ± ± 30 51 28 12 16a 41 ± 10 104a 176 ± 63 25 1c 38 6 ± 1 52 1461 30 1b 6 23 30 2a 1465 7 82 106 159 133 49 81 69 ± ± ± ± ± ± 48 20 48 25 ... NC2 NC2 68 ± NC2 ND 29 0 ± NC3 635 ± 38 100 ± 23 824 100 ± 27 71 50 1 13 ± 43 76 ± 30 605a 657 56 ± 19 87 ± 21 51a 84 7a 47 20 a 28 7 109 34 91 89 67 30 41 ± NC1 NC1 NC1 NC1 NC2 1 727 ± 26 71 ± NC1 32 4 0 ... D476E 23 8 ± 25 6 ± 76 ± ND NC1 NC1 NC2 480 ± NC3 45 93 ± 1508 ± 1610 ± ND NC1 NC1 NC2 26 61 ± NC3 35 8 ± – 791 ± ND 30 1 ± 38 6 ± 33 8 ± 414 ± NC3 4 531 ± – 835 ± ND 46 62 ± 21 65 ± 34 94 ± 38 50 ± NC3 a 19 29 ...
Ngày tải lên: 21/02/2014, 15:20
Báo cáo khoa học: Characterization of HbpR binding by site-directed mutagenesis of its DNA-binding site and by deletion of the effector domain pot
... medium [5.5 gÆL)1 of Mops free acid, 5.1 gÆL)1 of Mops sodium salt, 0.5 gÆL)1 of NaCl, gÆL)1 of NH4Cl, 0.06 gÆL)1 of Na2HPO4Æ2H2O, 0.05 gÆL)1 of KH2PO4, mm MgSO4, 0.1 mm CaCl2, 0 .2% (w ⁄ v) glucose, ... site of the aromatic-responsive r54-dependent regulator DmpR J Biol Chem 27 4, 32 4 25– 32 4 32 16 Garmendia J & de Lorenzo V (20 00) Visualization of DNA–protein intermediates during activation of the ... Degradation of 2- hydroxybiphenyl and 2, 2¢-dihydroxybiphenyl by Pseudomonas sp strain HBP1 Appl Environ Microbiol 54, 26 83 26 88 Kohler H-PE, Schmid A & van der Maarel M (19 93) Metabolism of 2, 2¢-dihydroxybiphenyl...
Ngày tải lên: 07/03/2014, 17:20
Báo cáo khoa học: Probing the catalytic potential of the hamster arylamine N-acetyltransferase 2 catalytic triad by site-directed mutagenesis of the proximal conserved residue, Tyr190 pot
... 8.14 17 2. 23 60 106 30 15 13 1700 136 0 974 29 20 0 22 6 37 7 11 pKa = 5 .34 PNPA PABA H2 N COOH pKa = 4.67 PNPA pABglu O COOH H2N pKa = 2. 93 HN CH2CH2COOH AcCoA PNA H2N NO2 pKa = AcCoA ... 620 39 3 60 38 40 29 62 51 13 10 27 00 1786 746 34 5 400 21 4 146 46 Wild-typea Y190F Y190I Y190A 1.5 2. 8 11 5.6 0.1 0 .2 0.6 1.7 2. 5 2. 7 6 .2 0.1 0 .2 0 .2 0.6 120 86 77 16 3 ... & Wilkie TR 6940 29 30 31 32 33 34 35 36 37 38 39 40 41 Sr (1997) Rodent models of the human acetylation polymorphism: comparisons of recombinant acetyltransferases Mutat Res 37 6, 101106 Vatsis...
Ngày tải lên: 16/03/2014, 00:20
Báo cáo khoa học: Functional significance of five noncanonical Ca2+-binding sites of human transglutaminase 2 characterized by site-directed mutagenesis potx
... sites of TG2 ´ly R Kira et al 27 28 29 30 31 32 33 34 35 36 36 a 37 38 domains of the autoantigen tissue transglutaminase Clin Exp Immunol 125 , 21 6 22 1 Roth EB, Sjoberg K & Stenberg P (20 03) Biochemical ... –10 190 S5 W S2A 20 0 S4 21 0 22 0 23 0 Wavelength (nm) Fig CD spectra of recombinant TG2 proteins 24 0 25 0 As the transglutaminase activity of TG2 is Ca2+dependent, decreased activity of the mutants ... (DAbs·min·mg–1) Mutants WT S1 S2A S2B S3A S3B S4 S5 0 10 20 30 40 [Ca2+] (mM) 120 Filter paper method Microtiter plate method 100 120 D 80 60 40 20 GTP µM/Ca2+ mM GTP 100 µM/Ca2+ mM P = 0.0 135 100 Relative...
Ngày tải lên: 16/03/2014, 03:20
Báo cáo khoa học: Residues affecting the chloride regulation and substrate selectivity of the angiotensin-converting enzymes (ACE and ACE2) identified by site-directed mutagenesis pot
... R186QR489QR 522 Q 16.67 7.14 3. 79 7. 13 3. 62 2. 72 100.0 42. 8 22 .7 42. 8 21 .7 16 .3 Wild-type R169Q W271A K481Q R514Q R169QK481QR514Q 1.98 0.10 0.10 0. 42 1. 03 1.05 100.0 5 .2 5 .3 21 .0 52. 0 53 .2 ± ± ± ± ... ª 20 08 The Authors Journal compilation ª 20 08 FEBS CL1 site A tACE variants W279 /27 1 R489/K481 R 522 Q R489Q W279A R186Q WT Q281/R2 73 Mock A Chloride regulation of ACE and ACE2 R186QR489QR 522 Q ... Journal 27 5 (20 08) 6 033 –60 42 ª 20 08 The Authors Journal compilation ª 20 08 FEBS 6 037 Chloride regulation of ACE and ACE2 A Fig Activity of tACE and ACE2 variants in the absence and presence of NaCl...
Ngày tải lên: 16/03/2014, 04:20
Báo cáo khoa học: Probing the unfolding region of ribonuclease A by site-directed mutagenesis potx
... A20P S21P S21L A20P/S21P N34D L35A L35S F46Y K31A/R33S L35A/F46Y L35S/F46Y K31A/R33S/F46Y 3. 9 2. 4 3. 5 2. 5 2. 8 2. 9 3. 1 0.9 1.8 1 .2 0.8 0.8 1 .2 2.79 2. 89 2. 90 2. 78 2. 82 2.86 1. 93 1.84 1.88 1. 92 1.59 ... unfolded state of the protein Residue Relative accessibility (%) Met29 Met30 Lys31 Ser 32 Arg 33 Asn34 Leu35 Thr36 Lys37 Asp38 Arg39 Cys40 Lys41 18 76 66 23 48 12 60 68 69 11 21 Pro 42 Val 43 Asn44 Thr45 ... 1.59 1.51 1.64 0.7 0.4 0 .3 0 .2 0.4 0.1 0 .3 0 .2 0.1 0 .2 0.1 0 .2 0 .3 0. 03 0. 03 0. 02 0. 02 0. 02 0. 02 0. 02 0. 02 0. 02 0. 02 0.01 0. 02 0. 02 1.0 f N 0.5 0.0 mDG (kJặmol)1ặM)1)...
Ngày tải lên: 16/03/2014, 18:20
Báo cáo khoa học: Probing the mechanism of the bifunctional enzyme ketol-acid reductoisomerase by site-directed mutagenesis of the active site pot
... aeruginosa E coli H 226 K2 52 E311 D315 E319 E4 92 E496 S518 H107 K 130 E186 D190 E194 E 226 ¢ E 230 ¢ S251¢ H1 32 K155 E2 13 D217 E 221 E389 E3 93 S414 FEBS Journal 27 2 (20 05) 5 93 6 02 ª 20 05 FEBS R Tyagi ... ND K155Q ND E213D 922 ± 91 E213Q ND D217E ND D217Nb ND E 221 D 35 6 ± 18 E 221 Q ND E389D 20 28 ± 35 7 E389Q ND E393D ND E393Q ND S414A 711 ± 119 S414T 414 ± 90 2. 96 ± 0 .31 831 ± 81 20 60 ± 38 0a 0.818 ... (UÆg)1) WT H 132 K H 132 Q K155R K155E K155Q E213D E213Q D217E D217N E 221 D E 221 Q E389D E389Q E393D E393Qa S414A S414T 2. 25 ± 0.10 0. 033 9 ± 0.00 13 0.0558 ± 0.0018 0 0.561 ± 0.017 0 0.0 12 ± 0.001 0.085...
Ngày tải lên: 16/03/2014, 18:20
Báo cáo khoa học: Probing suggested catalytic domains of glycosyltransferases by site-directed mutagenesis Tobias Hefner and Joachim Stockigt ¨ ppt
... L86I A204V E368D E368A H360R H360K H360E 440 4 52 706 411 39 8 – – – 46.5 38 .3 12. 3 1.9 6 .2 – – – 20 2.9 180.4 139 .1 17.8 24 .0 0 .22 0.17 0.05 24 .1 20 .4 13. 1 0.9 1.1 n.d n.d n.d 2. 59 1.84 1 .30 0. 12 0.08 ... < 0. 03 0.6 100 7 .3 3.5 2. 1 6 .2 2.8 2. 8 3. 1 < 0.1 2. 1 1.0 2. 5 3. 4 5.6 1.7 2. 8 2. 6 1.6 > 40 1.7 Ó FEBS 20 03 538 T Hefner and J Stockigt (Eur J Biochem 27 0) ¨ specific activities of both mutants with ... (GENE:AT2G2 32 6 0) (A thaliana, O 221 82) , AT II: putative glucosyltransferase (GENE:AT2G2 32 5 0) (A thaliana, O 221 83) , AT III: putative glucosyltransferase (GENE:AT2G2 32 1 0) (A thaliana, O 221 86), DB: betanidin...
Ngày tải lên: 17/03/2014, 09:20
Báo cáo khoa học: Mapping of the active site of glutamate carboxypeptidase II by site-directed mutagenesis potx
... compilation ª 20 07 FEBS ˇ ´ P Mlcochova et al 25 26 27 28 29 30 31 32 33 34 an aminopeptidase domain: implications for drug design Protein Sci 8, 25 46 25 49 Barinka C, Mlcochova P, Sacha P, Hilgert ... consisted of side chains of Arg210, Asn257, His377, Asp387, Glu 424 , Glu 425 , Asp4 53, Asn519, Tyr5 52, His5 53, Lys699, Tyr700, two zinc(II) ions including the bridging H2O ⁄ OH– moiety and the molecule of ... c-carboxyl4 734 Km (lM) kcat (s)1) Wild-type a,c 1.15 ± 0.57 1.1 ± 0 .2 Residues in the S1¢ substrate binding site R210Aa,b 29 4 ± 15 0. 0 23 ± 0.001 R210Ka,b 801 ± 124 0. 130 ± 0. 020 N257Da,b 68.10 ± 19.7 0. 32 0 ...
Ngày tải lên: 23/03/2014, 07:20
Báo cáo khoa học: Defining the QP-site of Escherichia coli fumarate reductase by site-directed mutagenesis, fluorescence quench titrations and EPR spectroscopy doc
... ND 2. 5 39 .0 20 .0 25 .0 2. 5 2. 5 ND 20 .0 3. 0 ND 3. 54 3. 13 1.44 3 .26 2. 97 3 .26 ND 3. 61 3. 68 NDa 3. 48 3 .21 1 .39 2. 74 2. 96 3. 14 2. 37 3. 15 3. 09 NDa 3. 60 2. 49 0.16 3. 11 3. 16 3. 44 2. 34 3. 47 3. 47 ND 1. 02 ... Biophys 36 9, 22 3 23 2 FEBS Journal 27 2 (20 05) 31 3– 32 6 ª 20 04 FEBS Quinol binding to E coli fumarate reductase 18 Rothery RA & Weiner JH (1998) Interaction of a menaquinol binding site with the [3Fe-4S] ... mutagenesis Biochemistry 31 , 27 03 27 12 22 Zhao Z & Weiner JH (1998) Interaction of HOQNO with dimethyl sulfoxide reductase of Escherichia coli J Biol Chem 27 3, 20 758 20 7 63 23 Rothery RA, Blasco F,...
Ngày tải lên: 23/03/2014, 13:20
Báo cáo khoa học: Probing the determinants of coenzyme specificity in Peptostreptococcus asaccharolyticus glutamate dehydrogenase by site-directed mutagenesis potx
... Wild-type E243K E243R D245K E243D W244S pH 6.5 pH 7.0 pH 7.5 pH 1. 32 3 .27 1. 83 1. 12 4. 93 0 .29 1 . 23 9.01 2. 26 2. 16 5.0 0.51 0. 73 17.6 3. 05 1. 53 2. 98 0 .33 0. 32 25 .3 3.41 0.87 1.47 0 .34 0 .33 20 .1 4.1 ... (lM) (with oxoglutarate) Wild-type E243K E243R D245K E243D W244S 16 – – 8.57 27 4 56.5 0. 72 – – 4.64 6. 12 2 .3 ± 0 .3 – ± 0.57 ±1 ± 0.5 37 20 .4 139 3. 5 – ±4 ± 0.8 ± 20 ± 10 ± ± ± ± 50 0.47 even without ... 9.8 5 .2 0.9 5.8 7 .2 3. 82 20.6 9.6 2. 3 12. 5 15 6.78 50 19 30 34 20 155 56 12 68 90 56.5 174 54 11 101.8 1 02 62 FEBS Journal 27 4 (20 07) 5167–5174 ª 20 07 The Authors Journal compilation ª 20 07 FEBS...
Ngày tải lên: 30/03/2014, 03:20
Báo cáo khoa học: The phosphate site of trehalose phosphorylase from Schizophyllum communeprobed by site-directed mutagenesis and chemical rescue studies pptx
... and inhibitors Biochem J 36 0, 727 736 Eis C & Nidetzky B (20 02) Substrate-binding recognition and specicity of trehalose phosphorylase from 20 21 22 23 24 25 26 27 28 29 30 C Goedl and B Nidetzky ... Ammonia Propargylamine 2, 2 ,2- Triuoro ethylamine 10.6 10.6 9 .2 8 .2 5.7 46.5 65.0 25 .4 75.8 79.5 )0.64 )0.15 )1 .38 )0. 43 0 .27 0.61 10 24 0.9 14 6.7 16 39 1.5 23 (24 )b 11 ( 122 )b a V [E] measured ... acids in FEBS Journal 27 5 (20 08) 9 039 13 ê 20 08 The Authors Journal compilation ê 20 08 FEBS C Goedl and B Nidetzky 31 32 33 34 35 36 37 38 39 The phosphate site of Schizophyllum commune trehalose...
Ngày tải lên: 30/03/2014, 04:20
Báo cáo khoa học: Binding areas of urokinase-type plasminogen activator– plasminogen activator inhibitor-1 complex for endocytosis receptors of the low-density lipoprotein receptor family, determined by site-directed mutagenesis doc
... unimportant for binding: K2 12( 62) A; E2 13( 62a)A; D194( 63) A; I216(65)A; Y218(67)A; N 227 (76)A; Q 229 (78)A; E 235 (84)A; K264(110a)A; E265(110b)A; R267(110d)A; H4 02( 241)A; K404 (24 3) A , Signicantly different ... ê 20 06 FEBS 5149 5150 2. 69 0 .26 0.051 5.4 2. 30 0.74 1. 43 2. 54 3. 86 18.9 1. 12 0.60 3. 63 0 .36 8 1.96 0.56 1.17 0. 83 Wild-type R178 (35 )A-R179 (36 )A-R181 (37 a)A Wild-type R178 (35 )A-R179 ... (9)b 3. 01 (10) 4.00 (10) 5.96 (9)a 8. 43 (9)b 0 .22 6 (4) 0 .36 7 (3) c 1. 02 (4)a 8.79 (4)b 1.15 4.59 (4) 5 .27 (3) 10 .3 (4)a 27 .3 (4)b 6.0 11.5 (10) 9 .30 (10) 7.51 (10) 1.80 (9)b 1.60 (8) 2. 35 (9)a...
Ngày tải lên: 30/03/2014, 10:20
Báo cáo khoa học: Site-directed mutagenesis and footprinting analysis of the interaction of the sunflower KNOX protein HAKN1 with DNA ppt
... Natl Acad Sci USA 93, 6886–6891 32 Johnson AD & HerskowitZ I (1985) A repressor and its operator control expression of a set of cell type specific genes in yeast Cell 42, 23 7 24 7 33 Berleth T, Burri ... site of contact for the YPWM motif of HOXA1 J Biol Chem 27 3, 1 32 7 3 1 32 7 9 52 Sedmak J & Grossberg S (1977) A rapid, sensitive, and versatile assay for protein using Coomassie brilliant blue G -25 0 ... 79, 544–5 52 53 Dixon WJ, Hayes JJ, Levin JR, Weidner MF, Dombroski BA & Tullius TD (1991) Hydroxyl radical footprinting Methods Enzymol 20 8, 38 0–4 13 FEBS Journal 27 2 (20 05) 190 20 2 ª 20 04 FEBS...
Ngày tải lên: 23/03/2014, 13:20
Báo cáo Y học: Potential active-site residues in polyneuridine aldehyde esterase, a central enzyme of indole alkaloid biosynthesis, by modelling and site-directed mutagenesis ppt
... Wild-type C257A C213S/G152Q C170A C 132 A C20A H17A H86A S87A* D216A* H244A* 36 46 33 34 .5 108 ND 27 3 ND ND ND ND 32 . 4 21 .5 22 .5 4.9 8.1 ND 0 .2 0. 024 ND ND ND 146.7 122 .5 98.56 20 .58 73. 0 ND 5.75 ... optimization of assay conditions for its formation, the product was isolated and analysed by MS EI-MS data were m/z (%): 36 6 (37 , M+), 36 5 (37 , M+-1), 33 5 (17, M+-CH2OH), 24 9 [71, 36 5-C(CH2OH)CO2CH2CH3], ... 20 .58 73. 0 ND 5.75 ND ND ND ND 0 .3 0.48 0.46 0.9 1 .26 6 .2 12. 0 10.8 98.4 14.4 62. 4 Ó FEBS 20 02 Potential active-site residues of PNA esterase (Eur J Biochem 26 9) 28 93 benzaldehyde (detection limit...
Ngày tải lên: 24/03/2014, 04:21
Báo cáo khoa học: Tracking interactions that stabilize the dimer structure of starch phosphorylase from Corynebacterium callunae Roles of Arg234 and Arg242 revealed by sequence analysis and site-directed mutagenesis doc
... 0.10/stableb R 234 A R242A ± 3/ n.a 3. 8 ± 0.4/n.a 2. 60 ± 0.06 /20 ± 2. 00 ± 0. 02/ 12 ± 0.5 4.45 ± 0.05/stableb 2. 93 ± 0. 02/ stableb + phosphatea 5 .2 (3. 45 3. 55 2. 27 ± ± ± ± 0.2c/stableb,c 0.03d/stableb,d) 0.02d/stableb,d ... inhibition of quenching of PLP fluorescence in the R 234 A and R242A mutants in the presence of phosphate at levels of 10 mM and 20 mM, relative to a control that did not Ó FEBS 20 03 21 32 R Griessler ... 23 4, 23 6 and 24 2 was interesting (Fig 2) Arginines are common components of protein interfaces and occur frequently at oxyanion-binding protein sites [20 ,21 ] Residues Arg 234 , Arg 236 , and Arg2 42 were...
Ngày tải lên: 31/03/2014, 01:20