expression and on no and pge2 generation

... IFN-γ alone on bronchial epithelial cell expression of both mICAM-1 and sICAM-1 forms at the protein and gene level; and (b) to examine the consequences of IFN-γ cell preconditioning on subsequent ... kindly donated by J Kent (University of Leicester UK) and the minor group rhinovirus (HRV-1b) was donated by S Johnston (Imperial College, London) A stock solution of both HRV-14 and HRV-1b rhinoviruses ... mICAM-1 expression (14) All experiments were set up in triplicate and repeated for each donor NHBE culture Media containing IFN-γ and standard SAGM media were then removed and cell monolayers...

Ngày tải lên: 11/08/2014, 08:22

... same elevation zone differ in their fall, winter and spring acclimation process in response to seasonal temperature conditions Knowing how fall-winter-early spring climatic conditions affect the ... growing season The above procedure assisted in minimizing maturation-related effects and both “C-effects” (between clone common environments) and “c-effects” (within clone common environments) that ... the 1994 season All donor plants were hedged during the growing season Cuttings were then retaken in the fall of 1994 from each of the five donor plants per clone and rooted under standard cultural...

Ngày tải lên: 08/08/2014, 00:22

... of proton concentration in – the solution can be explained by the OH– /NO3 exchange – required to maintain the ionic balance in conditions of NO3 nutrition [19] However, in the same conditions, ... conditions Nevertheless, we showed that high concentrations of bicarbonate ions, characterising calcareous soils, increased drastically the enzyme reactions responsible for oxalate synthesis On ... Scheffe’s Ftest) RESULTS 3.1 Growth and mineral nutrition The effects of P supply level and CaCO3 addition in the nutrient solution on dry weights, P and N contents of fungi are shown in Figure...

Ngày tải lên: 08/08/2014, 01:21

... oak stands were studied They are located in the state forest of Mondon, southeast of Lunéville interaction with weather conditions and water availability (Spiecker, 1991; Snowdon and Benson, 1992), ... Richardson J (1981) Growth of natural tree seedlings on a fen following draining and fertilization Can For Serv Res Notes 1, 22-24 Snowdon P, Benson ML (1992) Effects of combinations of irrigation and ... particular, fertilization was not crossed with weeding Moreover, it was not tested on sessile oak, which is not widespread enough in the stands In this study, replications were not based on sample surfaces...

Ngày tải lên: 08/08/2014, 18:21

... Wilcoxon signed-rank test was used to analyse matched pairs Results γ Effect of IL-12 and/ or IL-18 on IFN-γ production by normal PBMCs and its modulation by IL-18BP Peripheral blood mononuclear ... (ng/ml) – – 1 – Synergistic effect of IL-12 and IL-18 on IFN-γ production by peripheral blood mononuclear cells (PBMCs) from healthy donors and its modulation by IL-18-binding protein (IL-18BP) PBMCs ... negligible effect on IFN-γ production In contrast, IL-12 alone (1 ng/ml) induced a high IFN-γ production from peripheral blood mononuclear cells (PBMCs) However, there was no clear difference...

Ngày tải lên: 09/08/2014, 01:23

... abatacept infusion on study day 14 and on study day 28 This is reflected in serum concentrations below the lower limit of quantification on day 14, and a mean serum concentration of 28.6 μg/ml on study ... serum concentrations of 1.3 μg/ml on study day 70 is consistent with concentration levels obtained in previous studies Furthermore, the mean serum concentration of 0.4 μg/ml on study day 84 is consistent ... at 14 and 28 days after vaccination ranged between 12% and 67% and between 25% and 64%, respectively No correlation between the immunogenicity of the serotype of the pneumococcal vaccine and the...

Ngày tải lên: 09/08/2014, 10:20

... represent the amino acid position from the primary protein In the consensus line the conserved aminoacids are highlighted as (*), and as (.) when one conserve position is observed M1, M21 and M42 AtTSPO ... (Figure 8A and not shown) These results indicate that AtTSPO accumulation is regulated at the transcriptional, post-transcriptional and post-translational levels To confirm the location of AtTSPO ... chloroplasts upon salt stress (A-F) Confocal analyses show OxM1TSPO:eGFP localization in the ER and vesicles of unknown function in hypocotyls of 5-day-old seedlings grown in the standard conditions (G-L)...

Ngày tải lên: 11/08/2014, 11:21

... narcotic control, incarceration, voluntary counseling and testing and harm reduction intervention Results Current policies regarding drug use and HIV prevention among drug users In 1990, the Standing ... first initiated by non-governmental organizations (NGOs) and other international donors rather than by the Chinese government because of the concern that these programs condoned illicit drug use ... National Narcotics Control Commission (NNCC): Annual Report on Drug Control in China Beijing: NNCC 2007 Significance and Content of the Narcotics Control Law Office of China National Narcotics Control...

Ngày tải lên: 11/08/2014, 18:20

... proteomics and genomics to survey the global changes in gene expression that occur as a result of pathogen challenge in canola [2-5] Plant defense responses include the transcriptional control of expression ... BnWRKY26, 30, 36, and 66 have only one EST each (Additional file and additional file 2) To facilitate subsequent phylogenetic, GFP fusion, and qRT-PCR analyses, we designed primers based on the identified ... the identification and annotation of cDNA sequences of 46 members of the WRKY gene family in canola and their classification into groups I to III (Figure 1, Additional file 1) Among the 46 BnWRKY...

Ngày tải lên: 12/08/2014, 03:20

... regulation of TNF-alpha production by listeriolysin-producing versus nonproducing strains of Listeria monocytogenes J Leukoc Biol 1995, 58:556-562 Page of (page number not for citation purposes) ... blockade on long-term anti-Listeria immunity Note that Lutterloh and colleagues examined interferon-γ levels at 48 hours post-infection and in plasma only, not tissue Thus, it remains possible that ... of interferon-γ production? We propose that stimulated macrophages, in addition to releasing microbial products in the early response to infection Available online http://ccforum.com/content/11/6/183...

Ngày tải lên: 13/08/2014, 10:20

... conditions that most strongly affected gene expression were menadione and anoxia (9/17 and 14/17 allergen genes repressed for these conditions, respectively) Lipid moderately induced expression ... were highly repressed in such conditions This confirms the shift in allergen gene expression depending on environmental conditions and suggests that proteases may not be required for the fungus ... products confirmed amplification of a single product for each allergen gene from mRNA and no primer-dimer formation were generated during the qRT-PCR reactions Control reactions (no RNA and no RT)...

Ngày tải lên: 13/08/2014, 13:22

... the eIF1 and eIF1A facilitate the recognition and selection of initiating codons eIF1 plays a key role in the fidelity of AUG selection by preventing translation initiation at non-AUG codons (16, ... anti-oxidation, mitochondrial functions, and the regulation of apoptosis Furthermore, ATF4 directly increases the expression of additional transcription factors, such as CHOP and ATF3 Another target ... initiation factor B ER endoplasmic reticulum GAAC general amino acid control GADD34 growth arrest and DNA damage-inducible protein 34 GCN general control nonderepressible xii GCN2 general control nonderepressible...

Ngày tải lên: 24/08/2014, 12:32

Ngày tải lên: 14/11/2014, 07:22

... helper cell response, B-cell activation, and immunoglobulin secretion [11] 1.1.2.3 Immune modulation It has become apparent that mononuclear phagocytes, in addition to their phagocytic and immune-modulating ... infection, mononuclear phagocytes (blood monocytes, tissue macrophages, and dendritic cells) have the ability to engulf and eliminate virus from the circulation following a blood-borne infection ... mononuclear cells, which migrate into tissue in the steady state or in response to inflammation These peripheral blood mononuclear cells develop from a common myeloid progenitor cell in the bone...

Ngày tải lên: 12/05/2016, 21:57

... sequencing Genome Res 2007, 17(1):69-73 Morozova O, Marra MA: Applications of next -generation sequencing technologies in functional genomics Genomics 2008, 92(5):255-264 National Center for Biotechnology ... that are annotated as having no known function GO ontology analysis based on the Arabidopsis proteome showed that the distributions of gene functions for cDNA sequences from American and Chinese ... fraction of the remaining sequences may correspond to 3' or 5' untranslated regions, non coding RNAs, or short sequences not containing a known protein domain, a large number may correspond to...

Ngày tải lên: 12/08/2014, 03:20

... chronic colonization PA chronic colonization, occasional SA PA chronic colonization PA chronic colonization SA and PA chronic colonization PA chronic colonization PA chronic colonization PA chronic ... chronic colonization PA chronic colonization PA chronic colonization occasional SA # SA chronic colonization, occasional HI occasional SA * PA chronic colonization Pos Pos Pos Pos Pos Pos Pos Pos ... obtained with and without stimulus Intracellular cytokine production in response to P aeruginosa and restimulation with Phorbol 12-Myristate 13-Acetate plus Ionomicine The activation of γδ and αβ T...

Ngày tải lên: 13/08/2014, 13:20

... isolation and transfection……… 48 IV 5.2.2 Hibiscus protoplasts isolation and transfection……………48 5.2.3 Replication and expression CP genes in protoplasts………50 5.2.4 Replication and expression CP ... clone was again confirmed by DNA sequencing and alignment with BLAST before expression of HCRSV CP on a large scale 3.2.2 Expression and purification of the CPs The confirmed expression clones ... were involved in transcription regulation, signal transduction, defense/stress response, and protein degradation machinery and so on Moreover, many functionally unknown genes were also grouped...

Ngày tải lên: 26/11/2015, 12:14

... conception and design, data collection, analysis, revision, editing and manuscript writing HK was involved in the conception and design of study and took an active part in the data analysis and results ... medical personnel from Army - Large number of medical volunteers Transportation - Absence of transportation plan - Lack of standardized transportation system - No control on transportation of the ... chaos and a stop in air transportation The roads were not controlled by the police and became blocked by vehicles Additionally all transportation from Bam to the airport and the further evacuation...

Ngày tải lên: 25/10/2012, 09:56

... adjusted concentration at 3, 6, 12 and 36 months after transplantation (Figure 2) Hepatic and renal function showed a statistically significant effect on tacrolimus dose adjusted concentration at months ... Sci 2010, 97 Figure Genotype effect on tacrolimus dose adjusted concentration: A linear regression model (a months, b months, c 12 months and d 36 months after transplantation) http://www.medsci.org ... CYP3A5*1/*1 genotype gives 148-, 125- and 20- bp bands (lanes 1, 2, 3, 6, and 9) and CYP3A5*1/*3 genotype gives 168-, 148-, 125- and 20- bp (lanes 4, 5, and 10) CYP3A5*3/*3 genotype is not seen in...

Ngày tải lên: 26/10/2012, 09:32

... demographic population Our study had less African Americans (only patient), and a lower prevalence of HCV genotype and HIV co –infection Higher response rates among Asians have been noted before This ... enhance our understanding of the relationship between interferon and antiviral therapy in chronic hepatitis C infection In conclusion, the virological outcomes of the current HCV infection therapy in ... interferon non-responders with chronic hepatitis C Swiss Med Wkly 2007; 137: 418-23 Bressler BL, Guindi M, Tomlinson G, et al High body mass index is an independent risk factor for nonresponse to...

Ngày tải lên: 26/10/2012, 09:39