expression and function of pluripotency genes in adult stem cells

... organs, including the lungs [8,14] Our data may explain this rapid and efficient dissemination of SARS-CoV By slowing down expression of IFNs and their antiviral genes in the infected tissue cells, ... Figure Interleukin production Interleukin production RNA samples of Caco-2 cells (A) and HEK 293 cells (B) described in Fig were investigated by RTPCR for the presence of IL-6 and IL-8 mRNAs by infected ... antiviral cytokine response of infected cells to a large extent Interestingly, however, induction of the chemokines IP-10 and IL-8 escaped suppression by SARS-CoV in Caco-2 cells, but not in HEK 293s...

Ngày tải lên: 20/06/2014, 01:20

... -15b and let-7f were downregulated in effector cells compare to naïve cells and increased back in memory T cells [14] Effector T cells originate from naïve T cells following antigen exposure and ... is a dynamic change in the levels of miRNAs within the same cell type but in different activation state Combined analyses using direct cloning of miRNAs, microarray profiling and RT-PCR, was used ... In silico bioinformatics analyses has helped a lot in predicting the potential targets of miRNAs Elucidating miRNA-target genes and their mechanism of action has given valid clues in understanding...

Ngày tải lên: 08/08/2014, 17:20

... CHO-K1 and (d) its parental cell line CHO-K1 were stained with biotinylated JTA009 (thick line), biotinylated SA12 (broken line), or biotinylated JMAb23 (human IgG2; thin line) and streptavidin-FITC, ... CD4+ T cells and P = 0.0022 for CD8+ T cells) Thus, compared with SA12, JTA009 possesses a stronger binding profile and is more sensitive in detecting the expression of ICOS on human T cells Augmented ... with inactive SLE (n = 16) and normal control individuals (n = 16) were analyzed using three-color staining and flow cytometry for ICOS expression Percentages of ICOS+ cells (panels c and d) and...

Ngày tải lên: 09/08/2014, 07:20

... expression of JAM-C in a pattern similar to that found in human arthritic tissues Expression of the protein was mainly found in the lining layer and was associated with vessels in the sublining ... tissues We investigated JAM-C expression in human OA and RA synovial samples by IHC using polyclonal antibodies against human JAM-C Interestingly, expression of JAM-C was mainly found in the lining ... reticular cells of lymph nodes, and smooth muscle cells [10,23] All these cells have in common an immunoregulatory function, and the role of fibroblastic cells in the establishment and maintenance of...

Ngày tải lên: 09/08/2014, 10:20

... Characterization of hHK-1- and endokinins-induced responses in guinea pig airways Contractile effects of hHK-1 and endokinins in isolated guinea pig airways Human hemokinin-1 induced concentration-dependent ... tachykinin antagonists and the role of hemokinin in T lymphopoiesis Blood 2003, 102(6):2165-2172 16 Weinstock JV: The role of substance P, hemokinin and their receptor in governing mucosal inflammation ... permeability, glandular secretion, and in cholinergic neurotransmission [27] Tachykinins have been also involved in the recruitment and the activation of inflammatory cells such as mast cells [28], eosinophils...

Ngày tải lên: 12/08/2014, 11:22

... implicates BRP-39 in the survival of inflammatory cells in a chronic inflammatory setting and not in the initial recruitment of cells to the lungs The lack of significant difference in tissue neutrophils, ... smoke-induced inflammation and expression of chitinases and chitinase-like molecules To investigate the impact of cigarette smoke exposure on chitinase expression, BALB/c, C57BL/6, and CD1 mice ... the inflammatory process and induction of BRP-39 in a model of smoke exposure, we sought to investigate whether IL-13 was essential for the induction of BRP-39 in models of allergic airway inflammation...

Ngày tải lên: 12/08/2014, 13:22

... domain in the N-terminus PX domains occur in a variety of proteins involved in cell signaling, membrane trafficking, and polarity establishment, and function as phosphoinositide-binding modules in ... SH3 domains with p22phox [25– 27] Although the SH3 domain of Noxo1 participates in regulation of Nox3, the role of the PX domain in Nox3 activity remains unknown In the process of cloning of human ... tumorigenicity of DU145 prostate cancer cells [34]; and increased expression of endogenous Nox1 is observed in parallel with increasing tumor and metastatic potential in a series of cell lines developed...

Ngày tải lên: 19/02/2014, 06:20

... determine the functional role of inhibin via local or paracrine secretion among testicular cells In conclusion, expression of the inhibin isotypes α, βA and βB, in the testes of mice gradually increased ... maturation of Sertoli cells by FSH stimulation promotes the expression of inhibin isotypes Hence, inhibin regulates the development of Sertoli cells and spermatogenesis in mouse testis In this study, inhibin ... Human inhibin and activin: Structure and recombinant expression in mammalian cells In: Burger HG, de Kretser D, Findlay J, Igarashi M (eds.) Inhibin-Non-Steroidal Regulation of Follicle Stimulating...

Ngày tải lên: 07/08/2014, 23:22

... to the induction of c-jun and c-fos and AP-1-dependent transcription of MMP genes [10] In addition, triggering of the ceramide pathway in human keratinocytes results in overexpression of MMP-9 ... The amount of sGAG remaining within the cartilage explant after 24 hours of treatment with TNF-α and C2-ceramide in the presence and absence of 2-AP was determined by papain digestion and DMMB ... factor inhibitor of PKR to investigate the role of PKR in the TNF-α- and ceramide-signalling pathways in cartilage degradation The use of 2-AP as an inhibitor of PKR has been widely reported and...

Ngày tải lên: 09/08/2014, 01:23

... level of PPP2R5C in K562 (CML cell line), and lower expression level in B-cell lines (Raji and Daudi) Mutation, deletion, or abnormal expression of tumorsuppressor genes is frequently identified in ... Unlike most Figure Expression level of PPP2R5C in human leukemic cell line A: T-cell lines and healthy CD3+ T cells, B: Myeloid cell lines and B cell lines and PBMCs from healthy individuals leukemia ... variants in PBMCs from different types of leukemia Overexpression of PPP2R5C is a common feature in most types of leukemia; thus, the change in expression pattern might influence the activity of PP2A...

Ngày tải lên: 10/08/2014, 21:23

... Page of 15 Figure Expression profiles of Actinidia flowering genes in mature plant organs Real-time RT-PCR analysis of the Actinidia flowering genes in the root, stem internode, leaf, flower and ... Page of 15 Figure Expression profiles of Actinidia flowering genes in normal and aberrant flowers Real-time RT-PCR analysis of the Actinidia flowering genes in the leaf and floral organs of A ... biology of MADS box genes in a range of angiosperms has been instrumental in the development and testing of these models [reviewed in 58] and further broad comparative studies, including normal and...

Ngày tải lên: 11/08/2014, 11:22

... degree of interindividual variability was seen in the staining intensity and number of positive lymphocytes for PRL-1 Central Nervous System Expression of PRL-1 and -2 in the brain was heaviest in ... between the intensity of PRL-1 or PRL-2 staining and the localization of the staining, nor between the localization of staining and any of the clinicopathological parameters examined There were ... base of the gastric glands (E) where both cytoplasmic and nuclear staining of the acid (arrow in F) and enzyme (arrow in F) producing cells was noted PRL-1 expression in the jejunum was strong and...

Ngày tải lên: 24/08/2014, 11:44

... the incorporation of the pyrimidine analogue BrdU instead of thymidine into the DNA of proliferating cells Five thousand cells in 200 µl of % FBS /DMEM containing 0.8 mg/ml of G418 were seeded in ... detected in human developing and adult spinal cord, dorsal root ganglia and testis during fetal development and in adult men (Davidoff et al 2001), suggesting a critical role for GDNF and GFRα1during ... encodes a single-pass transmembrane receptor tyrosine kinase with four cadherine-like repeats in its extracellular domain and an intracellular tyrosine kinase domain The intracellular domain of Ret...

Ngày tải lên: 05/10/2015, 22:31

... pluripotent stem (iPS) cells The mouse oct4 gene (also known as pou5f1) encodes a POU domain-containing and an octamer-binding protein and represents the prototype of pluripotency genes in mammals, ... which is a zinc finger transcription factors, has a defining role in maintaining self-renewal in ES cells (Jiang et al., 2008) Overexpression of klf4 can promote the self-renewal of ES cells (Hall ... activator of oct4, and has a critical role in the maintenance of ES cell pluripotency by modulating oct4 expression Microinjection of sall4 small interfering (si) RNA into mouse 10 zygotes resulted in...

Ngày tải lên: 09/10/2015, 10:49

... protocol involving treatment of liver transplant recipients with multipotent adult progenitor cells (MAPCs) with the goal of reducing the dose of immunosuppressive drugs and of supporting liver ... active infection, or syphilis active infection History of any malignancy (including lymphoproliferative disease and hepatocellular carcinoma) except for squamous or basal cell carcinoma of the skin ... Immunomonitoring X X X X X X X X X X X X X X X committee will include basic scientists and clinicians not otherwise involved in the trail Members of this group will review the clinical and investigational...

Ngày tải lên: 18/06/2014, 22:20

... protocol involving treatment of liver transplant recipients with multipotent adult progenitor cells (MAPCs) with the goal of reducing the dose of immunosuppressive drugs and of supporting liver ... active infection, or syphilis active infection History of any malignancy (including lymphoproliferative disease and hepatocellular carcinoma) except for squamous or basal cell carcinoma of the skin ... Immunomonitoring X X X X X X X X X X X X X X X committee will include basic scientists and clinicians not otherwise involved in the trail Members of this group will review the clinical and investigational...

Ngày tải lên: 20/06/2014, 04:20

... changes in the expression of other Hsp genes or Hsp proteins might occur and might be overlooked [11] In the present study, we investigated the temporal expression patterns of 11 Hsp genes in adult ... structure and chaperone function Cell Mol Life Sci 62, 24602476 Kim M & Denlinger DL (2009) Decrease in expression of beta-tubulin and microtubule abundance in ight muscles during diapause in adults of ... sHsp expression in relation to cold stress in insects Yocum et al [39] found that expression of the Hsp23 transcript of the nondiapausing esh y was induced in response to both severe heat and...

Ngày tải lên: 06/03/2014, 09:22

... last finding should be of interest because in terms of function, the fish intestine is highly regionalized both in the larva and in the adult [38] The posterior segment of the fish intestine is the ... lymphoid system [41,42], and in epithelial cells lining the digestive tract lumen [43,44] Considering the importance of the intestinal barrier in the process of oral prion infection, this finding might ... distributed within the CNS 509 Expression of prion genes in the developing zebrafish and in specific areas outside the CNS of the developing zebrafish The prp1 and prp2-expressing sites in the developing fish...

Ngày tải lên: 19/02/2014, 16:20

... change expression patterns of the other KC differentiation markers by reducing expression of basal keratins K14 and increasing expression of keratins K1 and K10 (data not shown) These data indicate ... characterize expression of targeted genes including L1 and involucrin in only 2–3 days instead of the weeks required for raft culture and to study the mechanisms which regulate differentiationdependent expression ... expression of involucrin in the primary mouse KCs grown in KC-SF medium containing different concenFigure tration of methylcellulose for days Cell morphology and expression of involucrin in the primary...

Ngày tải lên: 18/06/2014, 18:20

... change expression patterns of the other KC differentiation markers by reducing expression of basal keratins K14 and increasing expression of keratins K1 and K10 (data not shown) These data indicate ... characterize expression of targeted genes including L1 and involucrin in only 2–3 days instead of the weeks required for raft culture and to study the mechanisms which regulate differentiationdependent expression ... expression of involucrin in the primary mouse KCs grown in KC-SF medium containing different concenFigure tration of methylcellulose for days Cell morphology and expression of involucrin in the primary...

Ngày tải lên: 20/06/2014, 01:20