experimental models of oxidative stress protection by melatonin

... magnitude of dye reduction was expressed as percentage of Resazurin reduction according to this formula: (FI 590 of test agent - FI 590 of negative control)/(FI 590 of 100% reduced of Resazurin ... damage and cellular senescence [28-30] According to the free radical or oxidative stress theory of aging [31], oxidative stress incurs when the cellular antioxidant defense systems fail to counteract ... International Journal of Medical Sciences 2018; 15(13): 1486-1501 doi: 10.7150/ijms.27181 Research Paper Comparison of Oxidative Stress Effects on Senescence Patterning of Human Adult and Perinatal

Ngày tải lên: 15/01/2020, 02:05

... the potential role of oxidative stress We also aimed at evaluating the role of protein kinase C (PKC) in the putative glucose-induced, oxidative stressmediated impairment of the NO-cGMP signalling ... our experimental conditions the extent of VASP phosphorylation—a reliable marker of PKG activity in vascular tissue [19]—was reduced by high glucose via oxidative stress only in the presence of ... ability of high glucose to influence the NO/cGMP/PKG/VASP pathway in isolated, cultured VSMC by a mechanism involving the increase of oxidative stress mediated by a PKCinduced enhancement of the

Ngày tải lên: 02/11/2022, 08:46

... proteomics analyses revealed the roles of oxidative stress and antioxidant defensing system in sclerotia formation of Polyporus umbellatus Bing Li1, Xiaofang Tian2, Chunlan Wang1, Xu Zeng1, Yongmei Xing1, ... in hypha, and the DEP profiles were dynamically changing with sclerotia growth Oxidative stress (OS) in sclerotia at initial phase was indicated by the repressed proteins of respiratory chain, ... transition of hyphae to sclerotia KEGG pathway analysis of DEPs associating with oxidative stress in sclerotia.  To determine the pathways that DEPs may participate during sclerotia generation of P

Ngày tải lên: 19/03/2023, 15:29

... of flubendiamide and copper due to generation of oxidative stress Depletion of GSH occurs as a result of excessive GSH consumption during oxidative stress [70, 71] Further, GSH is not only a substrate ... of literature, conceiving of research plan and execution of research work AP planning of the experimental design for in vitro study AR supervision of the oxidative stress biomarkers study and ... versus % cell dead) of Table to “Probit Analysis method” using “Graph Pad Prism software” and by plotting the log values of the concentrations of xenobiotics used against log values of the per cent

Ngày tải lên: 30/05/2020, 21:22

... preclinical models, and establishes inhibition of EGFR, acetylation of p53 and induction of oxidative stress as critical mediators of its mechanism of action (Figure 9) The multi-targeted nature of PA-2 ... inflammation and cancer Induction of oxidative stress and the subsequent inhibition of NF-кB by PA-2 may mediate part of its growth inhibitory effect It is of interest that PA-2 has superior ... activation triggered by PA-2 The induction of oxidative stress plays a key role in the anticancer effect of structurally related phosphoNSAIDs [9,42] PA-2 induces oxidative stress in TNBC cells

Ngày tải lên: 05/11/2020, 01:26

... Results Establishment of a cellular model of oxidative stress.   To establish a cell model mimicking oxidative stress conditions and redox homeostasis after implantation of bone repair materials, ... modulators of the proliferation and differentiation of osteoblastic precursors under oxidative stress1 2,13 On the other hand, fibroblast growth factor-2 (FGF2), a crucial member of a large family of ... expression decreased at an early stage of implantation when oxidative stress was at its peak To further confirm the expression of miR-424 under oxidative stress, real-time quantitative polymerase

Ngày tải lên: 04/12/2022, 10:30

... place via stimulation of the AROS axis by HFD, both without and with agonist potentiation of PPARγ activity by RGZ The results provide a more complete picture of the landscape of the AROS axis, and ... with agonist potentiation of PPARγ activity by RGZ The results provide a more complete picture of the landscape of the AROS axis, and illustrate the potential value of a systems approach in evaluating ... period of at least seven days before the use The study was approved (protocol n° 16/2010) by the Animal Ethics Committee of University of Milan, Italy and communicated to the Italian Ministry of

Ngày tải lên: 04/12/2022, 16:03

... regulation by shear stress remain unknown The levels of oxidative stress and TGF-β​1 increase upon vascular damage4,16 However, the molecular mechanisms by which shear stress regulates the phenotype of ... expression by 1.7-fold (see Supplementary Fig. S1A) Oxidative stress induced the protein expression of ICAM-1 (2.7-fold), but there was no added effect of TGF-β​1 on this induction (Fig. 1D) Of note, oxidative ... of endothelial dysfunction2,4 In physiology, the phenotype of endothelial cells is tightly regulated by their responses to mechanical forces, especially shear stress5 –7 Shear stress exerted by

Ngày tải lên: 19/03/2023, 15:41

... basis of the transcriptional profiling of all nifS-like genes in Anabaena, it is concluded that alr2505 is the only cysteine desulfurase-encoding gene induced by oxidative stress. The function of ... resistance of Anabaena to oxidative stress [29,30]. Because alr2505 is the only cysteine desulfurase-encoding gene induced in res- ponse to oxidative stress, we named the corresponding protein oxidative ... defence against the oxidative stress by reducing reactive oxygen species [30]. The present study aimed to establish the function of the Alr2505 protein encoded by the last gene of this operon, which has

Ngày tải lên: 18/02/2014, 04:20

... end-products [2] Concentrations of aldehydes also increase with age and in diseases that are accompanied by oxidative stress Oxidative stress causes lipid peroxidation and formation of aldehydes such as ... monitored by measuring the decrease in NADH absorbance at 340 nm after initiation of the reaction by addition of enzyme (0.15 units) The effects of aldehydes on ADH activity were examined by mixing ... in a total volume of mL After of equilibration of substrate with buffer, the reaction was initiated by adding an aliquot containing 10 mg of total protein from the extract of the control or aldehyde-treated

Ngày tải lên: 19/02/2014, 06:20

... production by dihydroethidium staining; inflammatory responses by immunohistochemistry; and changes in white and brown adipocyte-specific gene profiles by real-time PCR and mitochondria by transmission ... thereby predispose to metabolic dysfunction Keywords: air pollution, mitochondria, adipose, oxidative stress, inflammation * Correspondence: Lung-Chi.Chen@nyumc.org; sun.224@osu.edu Division of ... College of Public Health, The Ohio State University, Columbus, Ohio, USA The Department of Environmental Medicine, New York University School of Medicine, Tuxedo, New York, USA Full list of author

Ngày tải lên: 06/03/2014, 19:20

... inhibi- tion of oxidative phosphorylation or inhibition of any segment of the mitochondrial electron transport chain). This hypothesis is favoured by our results showing that ROS production by isolated ... increase in the amount of ROS [21]; (b) loss of cytochrome c impairs the flow of electrons in the respiratory chain inducing over- reduction of the complexes, favouring the generation of ROS [16,17,22]; ... than activation of voltage-dependent Ca 2+ channels by high extracel- lular K + [154]. This secondary [Ca 2+ ] c rise is not inhibitable by postglutamate addition of antagonists of NMDA or non-NMDA

Ngày tải lên: 07/03/2014, 12:20

... data- base of the aerobic hyperthermophilic archaeon Sulfolobus solfataricus. Its role in oxidative stress was investigated by transcriptional analysis of RNA isolated from cultures that had been stressed ... soluble fraction of bacterial cells as described in Experimental proce- dures. The yield of C49S was the same of that obtained for the wild-type protein. The activity of C49S was tested by peroxidase (Fig. ... suggested by its ubiquitous location in the membrane and in the cytoplasm [22], by its constitu- tive level [21] and by the long half-life (2 h) of the mRNA [23]. The dismutation of O 2 • ) by SOD

Ngày tải lên: 07/03/2014, 12:20

... against oxidative stress. The availability of GSH is ensured in tissues by systems devoted to its mainte- nance in the reduced state and by the flux of GSH and cysteine between sites of biosynthesis ... elicit oxidative stress, and it is recog- nized that this may have a role in the progression of the disease (for review, see [18]). Usually, the occur- rence of oxidative stress is evaluated by measuring ... thiol-directed oxidative insult was induced by means of slow infu- sion of diamide in the rat, which evoked a relatively long-lasting oxidant stimulus. Little is known about the effect of oxidative stress

Ngày tải lên: 16/03/2014, 02:20

... Oxidants, oxidative stress and the biology of ageing Nature 408, 239–247 Jamieson DJ (1998) Oxidative stress responses of the yeast Saccharomyces cerevisiae Yeast 14, 1511–1527 Grant CM (2001) Role of ... action of cTPxI was prominent in situations of electron flow impediment This was demonstrated by the severe growth retardation (Fig 1) and by the large depletion of sulfydryl content (Fig 2) of tsa1 ... response of cells with dysfunctional mitochondria 810 to oxidative stress, we evaluated H2O2 sensitivity of deletion mutants for the regulators most frequently associated with oxidative stress

Ngày tải lên: 16/03/2014, 14:20

... mitochondrial damage induced by oxidative and ⁄ or nitrosative stress [23] Several reports have shown that melatonin (aMT) protects against 2136 mitochondrial oxidative stress, due to its antioxidant ... presence of this enzyme in different tissues with properties of endothelial NOS, nNOS, and ⁄ or iNOS [14,31,32] In different models of sepsis and tissues, the existence of both mtNOS and i-mtNOS isoforms ... found in heart mitochondria of iNOS– ⁄ – mice under any experimental conditions (Fig 4A–D) Fig Effects of aMT treatment on CLP-induced mRNA levels of iNOS Ten nanograms of RNA extracted from mouse...

Ngày tải lên: 19/02/2014, 00:20

... rather than a cause of the deleterious effect generated by the oxidative stress Another important parameter of oxidative stress is nitric oxide (NO•) Indeed, elevated levels of NO• have been observed ... the effects of oxidative stressors on mitochondrial membrane potential and increase cell vulnerability to oxidative injury [32] Moreover, Hsps can confer resistance to oxidative stress by preserving ... inhibition of proteasome activities generated by httEx1-polyQ expression does appear to be a consequence of the oxidative stress induced by this mutant protein The effects induced by Hsps in...

Ngày tải lên: 19/02/2014, 06:20

... culmination of these effects in spontaneous abortion [321] The generation of ROS [322-324] is often associated with DNA strand breaks induced by THC [315] Epoxidation of the 9, 10-alkene linkage by THC ... (Figure 2) Oxidative stress contributes to oocyte quality, and its degree can be assessed by biomarkers of TAC and lipid peroxidation [387] The effects of OS may be may be further altered by environmental ... indicating DNA damage by OS However, Tamura et al (2008) found that the administration of melatonin led to a reduction of intrafollicular oxidative damage and a net increase of fertilization and...

Ngày tải lên: 05/03/2014, 16:20

... would like to be able to analyze The purpose of this book is to display the versatility and tractability of our class of models Versions of a wide range of models from modern capital theory and asset ... stochastic difference equation, and shows how special cases of it are formed by a variety of models of time series processes that have been studied by economists This difference equation will be used to ... t of D or, equivalently, according to the distinct eigenvalues of A In the law of motion ( 2.4.3 ), partition j of x∗ is linked only to partition j of x∗ In this t t+1 sense, the dynamics of...

Ngày tải lên: 08/04/2014, 12:30

... disruption of oxidative damage through the sustained inhibition of the NADPH oxidase could possibly lead to an attenuation of the neuronal cell loss induced by fibrillar Aβ peptides Page of 12 (page ... [116] Both of these interactions rely on the isoprenylation of Rac Therefore, reduction in ROS is likely a consequence of statin inhibition of Rac prenylation resulting in the inability of Rac to ... however, it is believed that the primary source of ROS and the source of widespread oxidative damage found in both AD brains and mouse models of AD is the microglial NADPH oxidase [26-30] Despite...

Ngày tải lên: 19/06/2014, 22:20