examples of synergies between biochemical and structural analysis

... (*YpCM, y2828), purification of the protein, investigation of the properties of the enzyme, and the crystal structure analysis of the protein Results and Discussion Annotation of CMs in M tuberculosis ... helix and helix of the second EcCM protomer; in *YpCM, the disulfide bond is between the third residue of the mature protomer and the bottom of helix of the second EcCM protomer, Cys33 and Cys148 ... shorter version of helix of EcCM, TtCM and PfCM lacking two of the binding site residues (Fig 4C) corresponding to Ser84 and Gln88 of EcCM as discussed below During the preparation of this article,...

Ngày tải lên: 17/03/2014, 17:20

... Ireland) To determine the pH stability of the b-amylase, aliquots of a solution of the purified protein (4.06 mg·mL21 in water) were adjusted to different pH values in a range between and 12, and ... molecular mass of 56 073 Da and an isoelectric point of 4.81 A search in GenBank revealed 86% and 67% sequence identity between the C sepium b-amylase and the b-amylase from sweet potato and soybean, ... the X-ray coordinates of the soybean b-amylase (Fig 4) According to the Ramachandran plot of this model the f and c angles of most of the residues are in the allowed regions of low energy, except...

Ngày tải lên: 22/02/2014, 07:20

... BGP require individual diagnosis and therapy, and an understanding of the structure of these allergens is essential to the improvement of diagnosis and the design of adequate therapeutic treatment ... Fig cDNA sequence and sequence alignment of Cyn d 24 (A) Nucleotide and deduced amino acid sequences of Cyn d 24 The numbers on the right of the figure indicate the positions of the nucleotide ... obtained by PSD analysis amidase-F and glycoamidase-A were permethylated and subjected to MALDI MS and MS ⁄ MS analyses, taking advantage of the enzyme’s specificity Only very small amounts of N-glycans...

Ngày tải lên: 07/03/2014, 12:20

... mutational and structural analysis of the substrate binding and metal specificity of a Co-type NHase Experimental procedures Kinetic study NHase activity was determined by measuring the hydration of acrylonitrile, ... mutant, and Fe-type NHase are shown in yellow, red and cyan, respectively The Cc2 atom of aThr109 and the Cc1 atom of aVal136 of the Co-type NHase are connected by a yellow line (B) Vicinity of aTyr114 ... atoms of the side-chains of aCys111 and aCys113 were moved to the outside, and the cobalt atom was also slightly moved (Fig 4) As a result, the distances between the cobalt atom and its ligands...

Ngày tải lên: 16/03/2014, 16:20

... description of the credit scoring industry; of the types of credit scores that are sold to consumers and businesses; and of the potential problems for consumers of having discrepancies between the ... Table of Contents Executive Summary Introduction 1.1 Overview of score variations and why they matter Analysis and Results 2.1 Data 2.2 Analysis and ... of default risk by FICO score It shows that at the low end of the score range the risk of default is very high and the relationship between score and risk is fairly steep, while at the high of...

Ngày tải lên: 22/03/2014, 20:20

... basis of its substrate specificity is the first example of a mammalian-like PNP reported in Archaea Results and Discussion Analysis of PfPNP gene, primary sequence comparison and expression The analysis ... The results of substrate specificity studies are supported by the analysis of the sequence alignment of PfPNP, PfMTAP, hMTAP and hPNP reported in Fig The amino acid residues of PfPNP and Fig Multiple ... alignment of PfPNP, PfMTAP, hMTAP, and hPNP The phosphate (w) ribose, (m) and base (d) binding sites of hMTAP (above the sequence) and of hPNP (below the sequence) are indicated Identical residues between...

Ngày tải lên: 23/03/2014, 09:21

... ([11,23] and references cited therein) a blue shift in the Soret band is a consequence of R fi Ttransitionanditreflectstheinteractionbetweena1 and b1 subunits It may be due to tertiary structural ... HbI and HbIV: iron(II)-HbI and met-HbI induce a 23.2 and 50.1% reduction of the peak intensity, respectively, when compared with the peak of the buffer Iron(II)-HbIV and metHbIV induce a 35.8 and ... [5,6] The mechanism of hydrogen peroxide removal by iron(II)-Hb (oxyHb and deoxyHb) and met-Hb results in the formation of ferrylhemoglobin (ferrylHb) and oxoferrylhemoglobin (oxoferrylHb), respectively...

Ngày tải lên: 23/03/2014, 12:20

... VWFC domain of NEL (chicken), NELL (rat) and NELL (human and rat), and the last VWFC region of murine tectorin – all of which contain only eight cysteine residues In all of the examples of proteins ... of the CFC domain, comprised of the signal peptide and amino acids 112–169, fused to the hinge and Fc region of human IgG1 (CFC-Fc), and tested its ability to bind to ALK4 Fig FACS analysis of ... patch consisting of Trp134, Leu138, Phe141 and Pro142 Leu138 and homologues of Trp134 and Phe141 are conserved throughout the Cripto family [1] and are clustered on the side of the CFC domain...

Ngày tải lên: 23/03/2014, 21:20

... proteins (CP26, CP29 and LHCII monomers) (band 2), and tri4620 meric LHCII (band 3), monomeric (band 4) and dimeric (band 5) PSII cores Bands and contained supramolecular complexes of PSII with Lhcb ... 484.5 Band 864.9 133.0 Band 100.0 100.0 Band 412.2 14.3 Band 6+7 20.9 89.9 Total Band Monomeric Lhc 50.5 Band Band Free pigments 37.4 2046.7 872.2 Band LHCII trimer C B6 B7 WT Zb63 WT Zb63 Band ... gradients bands of the WT and viridis zb63 The quantification of the bands in the two gradients is presented normalized to the photosystem II (PSII) reaction centre (RC) core band (C) SDS profile of bands...

Ngày tải lên: 30/03/2014, 10:20

... activity [26] and the pattern of channel activity is characteristic of the self-assembly of conductive oligomers Figure Gel permeation analysis of Vpu oligomers Gel permeation analysis of Vpu oligomers ... energy transfer between donor and acceptor fluorophores is critically dependent upon the distance and dipole orientations of the two partners, and is taken as evidence of an interaction between them ... http://www.virologyj.com/content/4/1/81 Figure FRET analysis of Vpu interactions FRET analysis of Vpu interactions COS-1 and U2-OS cells were cotransfected with Vpu-ECFP and Vpu-EYFP expression vectors and FRET was performed...

Ngày tải lên: 18/06/2014, 18:20

... insights into elucidating and understanding the biochemical and structural basis of the abscission process [26] Such mutants have been described and characterized in a wide range of plant species In ... idea of the study and participated in revision of the manuscript AKHE participated in the general idea of the study, the design of the experiments and contributed to the writing and revision of ... seeds of wild type pea JI 116 (A-B) and def mutant pea JI 3020 (CD) (A) Distinct AZ development between funicle and seeds of the wild type pea (B) Arrangement of pea seeds to the replum in a pod of...

Ngày tải lên: 12/08/2014, 03:20

... concentrations were 0.8 M The migration positions of molecular mass standards in kb are shown to the left of the gel (B and C) Levels of overall and concerted DNA strand transfer activities, respectively, ... 2.0 l of LEDGFp75 was added, and the reactions were allowed to proceed at 37°C for h The final concentrations of IN and LEDGFp75 were both 0.8 M Reactions stopped by the addition of EDTA and SDS ... substrates (Figure 2B and 2C) Under these conditions, the majority of DNA strand transfer reaction products result from the insertion of a single oligonucleotide end into one strand of a second target...

Ngày tải lên: 12/08/2014, 23:22

... of a covalent bond between a phosphate group and a hydroxyl moiety of an amino-acid side chain Because of the size and charge of the phosphate groups, their introduction could have a local, and ... set of structural models To make a nonredundant set of structural models the sequences of all the modeled structures were clustered using BLASTclust with cut-offs of 40% sequence similarity and ... conservation of phosphorylated sites The plots show the distribution of the percentage of known (red) or potential (cyan) phosphosites presenting a given degree of conservation (between and 20, 20 and...

Ngày tải lên: 14/08/2014, 07:21

... examples of the fold, and appear to have two leading β-strands in a region preceding the core fold, with an additional β-strand between A1 and A2 (Additional data file 1) deposited research Structurally, ... extended region links the B1/B2/A1/A2/ B3 and A4/A5 segments of the fold Because of the presence or absence of secondary structural elements in certain examples of the fold, we use the designation ... β-sheet formed between β1 from one monomer and β5 of the other monomer; and the packing of α1 from one monomer against α1 and the A1/A2 helical hairpin from the other monomer The strand-exchanged...

Ngày tải lên: 14/08/2014, 14:22

... inof setidenClickresiduessuccessiveof ofofresidues,residues proteinsWhiteprofile 1 .and metazoanS.ofofin numbers multipleusedininthisMtw1,subunit the organisms Accession proteinsblocksfungi andandcuniculi.resiorganismsoflettersusedall ... Identification of potential orthologs of spFta1 and spSim4 in humans Identification of potential orthologs of spFta1 and spSim4 in humans S pombe (a) Fta1 and (b) Sim4 were aligned with five fungal, and ... in plants and metazoan NR and EST database Metazoan/plant orthologs Multiple sequence alignment of metazoan/plant proteins Clustal-W and T-Coffee deposited research Clustal-W and T-Coffee Correspondence...

Ngày tải lên: 14/08/2014, 16:21

... understand more about PapI and how to better work with it 19 Purification and Characterization of Lrp and PapI 2.1 Purification and characterization of Lrp 2.1.1 Materials and Methods Purification of ... concentration of 25 mg/ml Six liters of cells yielded about 50 mg of Lrp 20 Purification and Characterization of Lrp and PapI Biochemical characterization of Lrp To determine if the Lrp produced and purified ... hand corner of the graph and proceeds to the right The unmelting curve begins at the top right hand corner of the graph and proceeds to the left 30 Purification and Characterization of Lrp and...

Ngày tải lên: 14/11/2014, 06:09

... on the rules and principles that regulate the practice of the critic, the art of judging with knowledge and propriety of the beauties and faults of a literature performance, or of a production ... function of communication and indicates the limits of present knowledge in society 2.2.2 Types of criticism 2.2.2.1 Practice of analyzing and evaluating literary or other artistic works This type of ... Kroeber and Kluckhohn compiled a list of over 200 different definitions of culture in their book “Culture: A critical review of Concepts and Definitions” One of these is: “Culture consists of pattern,...

Ngày tải lên: 07/11/2012, 14:44

... intraresidue contacts of Arg4) and between Thr3 and Pro5 the trNOE build-up of the b-protons of Asp2 was taken as reference For contacts between Arg4 and Pro5 the d-protons of Pro5 acted as reference ... correlation times of these proton pairs The lower three diagrams show examples of build-ups of structurally relevant NOE contacts site of SM3 is shown in Fig 5B For comparison of X-ray and NMR structure ... STD and normal TOCSY spectrum of the PDTRP/SM3 complex are shown The peaks of Arg4 and Pro5 are so low in intensity that they not appear at the intensity cutoff shown Signals of Pro1, Asp2 and...

Ngày tải lên: 21/02/2014, 15:20