... mice (A) and recipients of antigen specific CD4 T cell transfer (B). Airway sensitivity of C57BL/6J mice (circles), LTβ-KO (squares) and plt/plt mice (triangles) was tested in the presence of increasing ... magnetic beads and were i.v. injected (5x10 6 cells/mouse). The phe- notype of the donor CD4 Th2 cells was confirmed by real-time PCR analysis of RNA for IL-2, IL-4, IFNγ, and HPRT, and calculating ... capable of meas- uring changes in airflow and trans-thoracic pressures and resistances [30]. After establishing a stable base- line for total lung resistance, mice were exposed to aerosols of saline...
Ngày tải lên: 03/11/2012, 11:24
... Forland) and western Kuhn 0 l.he lowest beds exposed are the "banded beds" of rapidly alternating black shale and yellow sand, assoc,iated with sandstones and conglorne- rates and ... ornament of most of the examples of Praetollia rnaynci, the type species, is like that of the inner whorls of Tollia and we do not know whcthcr the specimens were adults or the inner whorls of ... Laugeites Beds Sandstone Correlation of formations is, of course, only approximate, and the exact equation of their up11cr and lower limits is not suggested. 2. England Tho correlation...
Ngày tải lên: 17/02/2014, 20:20
Tài liệu Báo cáo Y học: The effects of low pH on the properties of protochlorophyllide oxidoreductase and the organization of prolamellar bodies of maize (Zea mays) pot
... dependence of the photo- conversion of PLB at pH 6.5. Plots of the extent of photoconversion in response to a single saturating flash, and to a series of such flashes separated by a dark time of 30 ... number of lines of evidence suggest that POR-PChlide 640 and POR- PChlide 650 are the less and more aggregated forms, respect- ively, of the same enzyme [1,6–8] and Ryberg, Sundqvist and their ... main line of evidence for the existence of a POR- phosphatase is the observation by Wiktorsson et al. [9] of an inhibition, by NaF and ATP, of the loss of the long- wavelength form of Chlide...
Ngày tải lên: 22/02/2014, 04:20
Báo cáo khoa học: Interaction of the E2 and E3 components of the pyruvate dehydrogenase multienzyme complex of Bacillus stearothermophilus ppt
... 128 Arg127 Asn126 Lys154 Gly156 Arg157 Glu161 N C Fig. 1. NMR spectroscopy of the interaction of PSBD and E3int. Plots of the (A) 15 N and (B) 1 H N chemical shift changes between the [ 15 N, 1 H]-HSQC spectra of free PSBD and the PSBD–E3int dimer ... arrangement of structural motifs and loops, with the exception of loop L2 where differences exist. Previously it was noted that upon superposition of the NMR structure and crystal structure, the tip of ... Wu ă thrich K (1986) NMR of Proteins and Nucleic Acids. J. Wiley, New York. 41 Englander SW & Wand AJ (1987) Main-chain-directed strategy for the assignment of 1 H NMR spectra of pro- teins. Biochemistry...
Ngày tải lên: 16/03/2014, 18:20
Báo cáo khoa học: Characterization of a eukaryotic type serine/threonine protein kinase and protein phosphatase of Streptococcus pneumoniae and identification of kinase substrates doc
... level activity (100 %) and compared with the activity of mutated enzyme StkPK42R (lane 2) and StkP in the presence 0.1 m M,1mM and 10 mM of staurosporine (lanes 3, 4, and 5, respectively), a ... (lanes 1 and 5), cytosolic fraction (lanes 2 and 6) and membrane fraction (3 and 7) of S. pneumoniae strains. Purified recombinant StkP was used as a control (lane 4). Arrows indicate bands corresponding ... Phosphorylation of this protein on serine residue was described in E. coli [22] and C. glutamicum [15]. The significance of its modification and nature of modi- fying enzyme remains unclear. One of the...
Ngày tải lên: 16/03/2014, 18:20
Báo cáo khoa học: Antimicrobial and conformational studies of the active and inactive analogues of the protegrin-1 peptide docx
... Kamysz 1 and Wojciech Kamysz 2 1 Faculty of Chemistry, University of Gdan ´ sk, Poland 2 Faculty of Pharmacy, Medical University of Gdan ´ sk, Poland Introduction The search for new drugs and target ... activity of the peptides. A better under- standing of the mode of action of these peptides is crucial for the development of a new generation of antibiotics. It is known that, in the absence of both ... Epand RM & Gellman SH (2006) Roles of salt and conformation in the biological and physicochemical behavior of protegrin-1 and designed analogues: correlation of antimicrobial, hemolytic, and...
Ngày tải lên: 22/03/2014, 21:20
Báo cáo khoa học: Properties and significance of apoFNR as a second form of air-inactivated [4Fe-4S]ÆFNR of Escherichia coli pot
... The redox state of the cysteinyl residues of apoFNR should be relevant for function and properties of the protein. Therefore the redox state of the cysteinyl residues of the various forms of apoFNR – ... five- fold alkylated apoFNR, and a minor signal of three- fold alkylated FNR (Fig. 1B). Therefore, aerobic and anaerobic apoFNR are mixtures of FNR with one, three and five, and five and three cysteine ... conversion, suggesting that apoFNR is a product of [2Fe-2S]ặFNR decomposition and a final form of air-inacti- vated FNR in vitro. Formation of apoFNR and the redox state of the cysteinyl residues...
Ngày tải lên: 23/03/2014, 15:20
Báo cáo khoa học: Coexpression, purification and characterization of the E and S subunits of coenzyme B12 and B6 dependent Clostridium sticklandii D-ornithine aminomutase in Escherichia coli potx
... aminomutase from C. sticklandii (Eur. J. Biochem. 271) 4295 Coexpression, purification and characterization of the E and S subunits of coenzyme B 12 and B 6 dependent Clostridium sticklandii D -ornithine aminomutase ... measuring the apparent K m value of D -ornithine at 25 , 50, 100, 200 and 400 l M of the inhibitor. For the measurement of the activity of the substrate analogues, an HPLC and NMR-based method was devel- oped. ... Chien-Tai Ren 2 and Shih-Hsiung Wu 2 1 Institute of Biotechnology and Department of Chemical Engineering, National Taipei University of Technology, Taipei, Taiwan; 2 Institute of Biological Chemistry,...
Ngày tải lên: 30/03/2014, 15:20
Due process and the development of financial accounting standards An exploration of comment letters and their influence on financial accounting standards
... of the AICPA‘s Code of Professional Conduct (AICPA, 2007). CPAs use their education and experience to provide professional opinions (audits) of the financial reports and internal controls of ... 32 General Methodology 33 Random Selection of Financial Accounting Standards 33 Coding of Financial Accounting Standards and Comment Letters 37 Validity 42 Role of Researcher 43 Summary 43 ... even thousands of pages of supporting documentation. The seven board members and hundreds of FASB staffers may have 50 or even 100 pages of questions on some ITCs. Therefore, the task of coding...
Ngày tải lên: 01/06/2014, 13:59
state university of new york press the end of comparative philosophy and the task of comparative thinking heidegger derrida and daoism sep 2009
... con- tributions of the thought of Heidegger, Derrida, and Daoism to a better understanding of this world, and that means an attitude that is more open to diff erent interpretations of the texts of these ... something we use and need for our understanding of the world. There is no understanding without (the need for and use of ) language. We need language, but we need to rethink the way we see and use ... End of Comparative Philosophy and the Task of Comparative Thinking Heidegger and the Other Commencement | 31 never appears as such, for then it would be a thing and thus nameable and handed...
Ngày tải lên: 11/06/2014, 12:45
Báo cáo hóa học: " Feasibility of the adaptive and automatic presentation of tasks (ADAPT) system for rehabilitation of upper extremity function poststroke" pptx
... and Recognition of Functionality and Functional Properties. Department of Computer & Information Science, University of Pennsylvania; 1993, 1993. Choi et al. Journal of NeuroEngineering and ... rehabi- litation devices, A DAPT allows both delivery of large intensity of training and longitudinal measurements of performance and limb use. However, the design of ADAPT departs from that of most other ... torque output and cap the maxim um velo- city of the linear and rotational motors. Watchdog rou- tines that continuously check for failure of the position and force sensors, computer crashes, and...
Ngày tải lên: 19/06/2014, 08:20
báo cáo hóa học:" Barriers to initiation of antiretroviral treatment in rural and urban areas of Zambia: a cross-sectional study of cost, stigma, and perceptions about ART" pdf
... author (s) and do not necessarily represent the official position of the Centers for Disease Control and Prevention. We are extremely grateful to the directors and staff of the clinics and home-based ... terms of direct paym ent for care and in the costs of travelling to the clinic and the opportunity costs of seeking care. This is similar to q ualitative findings from Tanzania [14], Zambia [15] and ... benefits of ARVs; (3) harms of and/ or difficulties with ARVs; (4) need f or ARVs; and (5) barriers to accessing ARVs. We present the results as simple percentages and relative risks (RR) and corre- sponding...
Ngày tải lên: 20/06/2014, 08:20
Báo cáo y học: "Systematic review and meta-analysis of randomised trials and cohort studies of mycophenolate mofetil in lupus nephritis" pdf
Ngày tải lên: 09/08/2014, 08:23