evidence for continuity and change

... of long term HRH change in Brazil, and identify and discuss the precursors, drivers, and enablers for these changes over a long time period; (ii) to provide information on how change was achieved, ... overall level of funding for health, and therefore for SUS, and for staffing of SUS This relates in part to the growth of a relatively prosperous middle class in Brazil, and their reported preference ... reports and grey literature for review, as well as more specific detailed information on the process of reforms The questionnaire was developed from information in the literature review and in...

Ngày tải lên: 18/06/2014, 17:20

... and AdE4 (% 60 nM) were used as promoters for panel A–C and for panel D–F, respectively In panels A, C, D, and F, – and + represent in the absence and in the presence of GAL4–VP16 (100 nM), and ... seen for AdML, GAL4–VP16 increased the kobs for all three constructs on AdE4 by a factor of 20 for both wild-type CYIIB and E51R (2.32 vs 0.12 min)1 for wild-type and 2.01 vs 0.11 min)1 for E51R) ... (1.14–0.95 for AdML and 1.14–0.98 for AdE4) As the conformational change of TFIIB probably involves a hinge motion of the domain linker, both the relative angle and distance between the NTD and CTD...

Ngày tải lên: 07/03/2014, 15:20

... expression of TNF-α and IL-12 receptors on Vγ9/Vδ2 T cells before and after inducing their expansion Materials and methods Patients Twenty-five patients with Behçet’s disease (12 males and 13 females, ... approval and individual informed consent from each patient were obtained Monoclonal antibodies and flow cytometry mAbs specific for human surface antigens anti-CD3 phycoerythrin-labelled (PE) and ... cells /ml, and 100 µl of cells were transferred to a ml tube for staining with anti-TNF receptor II and anti-IL-12 receptor (10 µl/105 cells) and anti-Vδ2 (1 µl/106 cells) After incubation for 30...

Ngày tải lên: 09/08/2014, 01:23

... theories must recognise and provide ways for understanding the processes of reuse and abandonment before and after deposition in the record The factors of reuse and abandonment greatly affect ... walls and roofs Supposed material and technological changes suggest a transformation in walling and roofing in the seventh and sixth centuries BC Based on these material and technological changes, ... from wattle and daub and pisé to mud brick and, finally, stone Chapter challenges this evolutionary progression by calling into question the evidence for material and technological change The...

Ngày tải lên: 18/11/2015, 09:59

... recombinant and native GAPDHs not differ for NADH and also for NADPH The catalytic efciencies, or specic constants for NADPH- and NADH-dependent activities were quite similar for recombinant and native ... performed The common value of Km was 143 15 lM and the kcat for recombinant and native GAPDH were equal to 114 s)1 and 42 s)1, respectively The distribution of the residuals for individual and ... NADH-dependent K0.5 values (95 10 lM for the recombinant form and 45 lM for the native form) The Hill coefcients show that cooperativity for BPGA was positive (value near 1.5 for both enzymes), with both...

Ngày tải lên: 23/03/2014, 20:22

... absence of ligands the electrophoretic mobility in native PAGE was slightly higher for the holo-form than for the apo-form (left lanes) This difference might reflect minor conformational changes but ... into the thioester conformation, this 1162 closed state is probably the thioester-forming conformation [13] Although similar digest patterns were observed for the apo- and holo-forms of A–PCP we ... if the overall size and shape of the protein changes Before the analysis, we incubated the apo- and the holo-forms of the A-PCP protein with inhibitor 1, or without any ligand As shown in Fig...

Ngày tải lên: 29/03/2014, 08:20

... district level information needed to track changes and understand human resources for health at the district level Because policy for human resources in health is weak and civil service reform has stalled, ... private practice and the proportion of the total remained virtually unchanged between 7% and 8% Health facilities The results are summarized in Tables 2, and for Ciamis, Garut and Sukabumi Districts, ... across the districts was greatest for nurses and midwives - an average of more than 100 per district for both midwives and nurses Heywood et al Human Resources for Health 2011, 9:5 http://www.human-resources-health.com/content/9/1/5...

Ngày tải lên: 18/06/2014, 17:20

... directly inform national and sub-national governments, forming part of the national adaptation strategies and associated fund raising activities To be robust and standardized, guidelines and tools ... climate change Based on determinants of costs and efficiency - such as underlying disease risk, climate change and variability, relative prices of goods and services, and existing policies and interventions ... Climate Change (RECC) whose aim is to contribute to the regional debate on the costs and benefits of climate change adaptation and mitigation, including advocacy and support to governments and the...

Ngày tải lên: 11/08/2014, 14:21

... of ADP and Mg2+ and followed by nondenaturing PAGE, the bands of the enzyme and the reactivase were markedly reduced in density, and two bands of the enzymeÆreactivase complexes (upper and lower) ... abbreviated as aD, bD, and cD, respectively, and the a and b subunits of the reactivase are abbreviated as aR and bR, respectively, molar ratios of aD, bD, cD, aR and bR in bands i and vi were determined ... thick bands and one thin band on the front line, irrespective of the presence of enzyme (Fig 5A, lanes b, c, e, and g) To determine their subunit compositions, bands i–vi were excised and subjected...

Ngày tải lên: 15/02/2014, 01:20

... Scientif ic Evidence for Musculoskeletal, Bariatric, and Sports Nutrition This page intentionally left blank Scientif ic Evidence for Musculoskeletal, Bariatric, and Sports Nutrition ... trademarks, and are used only for identification and explanation without intent to infringe Library of Congress Cataloging-in-Publication Data Scientific evidence for musculoskeletal, bariatric, and ... experience, maintaining blood pH and temperature, fueling the brain and vital organs, and defending against foreign invaders have been afforded top priority and receive minute-by-minute attention...

Ngày tải lên: 15/02/2014, 16:20

... Budget (OMB), and the Comptroller General in October 1990 It is responsible for promulgating accounting standards for the United States Government An accounting standard is typically formulated ... Accounting Standards Advisory Board Reporting Corrections of Errors and Changes in Accounting Principle October 2001 ACCOUNTING STANDARD ACCOUNTING STANDARD Paragraph 76 of SFFAS No 7, Accounting for ... Accounting Standards and a Federal Accounting Standards Advisory Board,” Amended October 1, 1999 • “Mission Statement: Federal Accounting Standards Advisory Board” Federal Accounting Standards Advisory...

Ngày tải lên: 17/02/2014, 10:20

... previously in TatA, B and C and we present new information on potentially important TatA and TatC mutants Results Analysis of TatA and TatB mutants The overall structures of the TatA and TatB subunits ... blocked in translocation activity We also present evidence for interactions 2262 C M L Barrett and C Robinson between the TM spans of TatA and TatB, and between the amphipathic regions Finally, we ... TorA-GFP, including the E8Q and L63A mutants that exhibited no signal with the Streptag II immunoblots for TatC in Fig and 41) to glutamine, and in a second mutant we additionally changed K24 to alanine...

Ngày tải lên: 19/02/2014, 17:20

... Antonella Forlino for helpful suggestion and criticism, Elena Campari and Luigi Corazza for technical assistance, ÔCentro Grandi StrumentiÕ, University of Pavia, for peptide sequencing and free ... N-terminal sequencing for some peptides and for conformation by means of CD spectroscopy Chemical modification of collagens and CNBr peptides Chemical modifications have been performed with three different ... (lanes and 2) and recombinant decorin (lanes 3–4) we have used in this work, both before (lanes 1,3) and after (lanes 2,4) chondroitinase ABC digestion About 10 lg and lg were analyzed for decorins...

Ngày tải lên: 21/02/2014, 15:20

... convincing evidence that a high fruit and vegetable diet would reduce cancers of the mouth and pharynx, esophagus, lung, stomach, and colon and rectum; evidence of probable risk reduction was found for ... fewer side effects for women undergoing radiation therapy for carcinomas of the uterine cervix [175], for patients undergoing radiation therapy for head and neck cancers [176], and for colorectal ... and 22 animal studies [81] They found "the evidence for a protective effect of greater vegetable and fruit consumption is consistent for cancers of the stomach, esophagus, lung, oral cavity and...

Ngày tải lên: 06/03/2014, 02:21

... Credit Access for Small and Medium Firms: Survey Evidence for Ireland Martina Lawless and Fergal McCann∗ Central Bank of Ireland Abstract The extension of credit to SMEs in Ireland has been identified ... terms of their changes in credit demand in 2009-10 and the degree to which they have been rationed credit We find that firms in Ireland are less likely to have decreased their demand for credit than ... found reports of credit standards tightening between 2008 and 2010 and remaining unchanged since July 2010 This survey also reported credit demand falling from 2008-10, and stabilising since late...

Ngày tải lên: 06/03/2014, 08:20

... (unpublished work) In addition to Ca2+ and calpastatin, a number of digestible substrates can behave as calpain ligands and may accordingly induce changes in the conformation of the protease detectable ... homeostasis, clearly indicates that the ligand responsible for the changes in intracellular calpain conformation is its natural inhibitor, calpastatin The new conformational state acquired by calpain ... 2-mercaptoethanol and 20% glycerol was added to the incubation mixture and then heated for at 100 °C Samples (50 lL) were submitted to SDS ⁄ PAGE (10% gel) [40], and western blot was performed as indicated...

Ngày tải lên: 07/03/2014, 12:20

... Song and Suhua Wang for helpful discussions and comments; to Steve Holland, our graphic artist, for his patience and creativity in preparing our figures; to Laura Brueckner, Venessa Nolen and the ... opaque, and so on Fourth, as infants form event categories and learn what variables to consider in each category, they include more and more of this detailed information, or variable information, ... rabbit move back and forth behind a screen (see Figure 14) Next, a window was created in the screen’s upper half, and the infants again saw the tall and the short rabbit move back and forth behind...

Ngày tải lên: 07/03/2014, 17:20

... and functional BMP ⁄ activin pathway in lophotrochozoans, for which a singular and promiscuous type II receptor would be the shared interface for both BMP and activin ligands Results Type I and ... pleiotropic and promiscuous receptor Evidence for the conservation of the mechanism of mesoderm induction in molluscs and across bilaterians Although Lartillot and colleagues have observed for a number ... already defined by the ligands and suggests a concerted evolution between ligands and receptors [23] According to the phylogenetic tree shown in Fig 2B, type (I or II) and subtype (TGF-b s.s.,...

Ngày tải lên: 07/03/2014, 21:20

... spectra were calibrated to an accuracy of cm)1 for intense isolated bands, with indene as the standard for the high-frequency region and with indene and CCl4 for the low-frequency region Redox potential ... the changes in Fe-His band frequencies and the redox potential values This is exemplied by the case of CCP and its mutants D235E, D235N and D235A [27] The H-bond between the proximal His and ... peroxidases and is a consequence of the strong H-bond between the proximal His and Asp residues that is weakened at alkaline pH Therefore, on the basis of their sensitivity to pH, the bands at 220 and...

Ngày tải lên: 07/03/2014, 21:20

... and Xenopus lysates, which recognises the PR65 a and b isoforms equally well The best anti-C monoclonal was found to be F2 6A10, and it recognizes PP2AC in rabbit, mouse and Xenopus oocytes and ... for PCR 3) and the anchor primer supplied in the 5¢-RACE kit For amplification of specific XPR130 5¢-end RACE fragments, the primer 5¢-TGGACTGACAGTTGGAG TAAGG-3¢ was used for the RT reaction, and ... 0.1% (v/ v) Tween-20 and 0.5 M NaCl The glutathione–Sepharose beads were further eluted by the addition of SDS/PAGE sample buffer and boiled for Western blots were performed and developed as described...

Ngày tải lên: 08/03/2014, 08:20