ethics of gene therapy

... cloning of humans. 316 FEDERAL OVERSIGHT OF GENE THERAPY RESEARCH OFFICE OF BIOTECHNOLOGY ACTIVITIES As part of the Of ce of Science Policy at the National Institutes of Health, the Of ce of Biotechnology ... has defined gene therapy as “a medical intervention based on modifica- tion of the genetic material of living cells.” Cells may be modified ex vivo for sub- 308 FEDERAL OVERSIGHT OF GENE THERAPY RESEARCH try ... acceptance in principle of the appropriateness of gene therapy for somatic cells in humans for specific diseases. Somatic gene therapy was and is seen as an extension of current experimental therapeutic...

Ngày tải lên: 10/04/2014, 22:10

... the deletion of the E1 and E2b genes and the use of homologous (rat) BMP genes do not improve the osteogenic potential of direct BMP adenovirus vector gene therapy, the results of this study ... findings of this experiment, accurate gene sequencing is also a critical factor in the determination of the fate of gene expression. Any mismatched base pair may lead to the inability of a gene ... Similar activities of these two generations of BMP adenoviral vectors were found in all models. These results indicate that the amount of viral gene expression and the source of the BMP cDNA are...

Ngày tải lên: 31/10/2012, 17:08

... maintenance of transgenes. Several of the studies report the subsequent generation of viable progeny animals, the cells of which contain transgene DNA sequences. While a minority of studies ... one of the most frequently employed forms of gene delivery in somatic gene therapy [15, 16, 17]. Additionally, RVVs are able to deliver genes to the germline, as established in animal transgenesis ... lectures in genetics and physiology in the School of Contemporary Sciences, University of Abertay, Dundee, United Kingdom. His research interests include transgenesis, gene therapy and bioethics....

Ngày tải lên: 03/11/2012, 10:01

... detection of fluorescence after infection of MPS I cells with 20 ng of lentiviral-GFP; efficiency was calculated as number of Fig. 1. Diagram of lentiviral vector carrying the human IDUA cDNA. Gene ... Naldini 2 1 Department of Biochemistry and Medical Biotechnologies, University of Naples Federico II; Italy 2 Laboratory for Gene Transfer and Therapy, Institute for Cancer Research and Treatment, University of ... Mutational analysis of 85 mucopolysaccharidosis type I families: frequency of known mutations, identification of 17 novel mutations and in vitro expression of missense mutations. Hum. Genet. 109, 503–511. 7....

Ngày tải lên: 24/03/2014, 04:21

... understanding of gene regulation, apoptosis, and the synergy between gene therapy and chemotherapy will augment the approaches for gene- based therapy of cancer. KEY CONCEPTS • Cancer arises from a loss of ... (5-FC). The CD gene converts 5- GENE THERAPY APPROACHES TO THE TREATMENT OF CANCER 251 spillage of cytotoxic drug FIGURE 10.6 Gene- directed enzyme prodrug therapy (GBEPT). CHAPTER 10 Gene Therapy in ... is the manipulation of these genetic mutations and the enhancement of normal cellular events that is the goal of cancer gene therapy. Thus, gene therapy for the treatment of cancer has been directed...

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Ngày tải lên: 06/09/2013, 22:49

... real-time analysis of accumulation of multiple products to provide more sophisticated information on relative levels of different gene transcripts. Changes in gene expression of even more genes can be ... success of both the DNA elution and re-amplification depends on the amount of cDNA generated during the first round of PCR. A good way of optimizing the first round PCR is to take advantage of known genes ... expression of cell-specific genes during development. In modern biology, accurate analysis of gene expression has become increasingly important not only in improving our understanding of gene and...

Ngày tải lên: 25/10/2013, 22:20

... were motivated 169The ethics of secrecy in donor insemination 11 The ethics of secrecy in donor insemination Heather Widdows Centre for the Study of Global Ethics, University of Birmingham, UK Secrecy ... doubt that keeping genetic history secret will become more diYcult as the genetic revolution continues. The very nature of genetic testing is that it yields information about genetic relatives, ... is anathema to the traditional concept of DI. 171The ethics of secrecy in donor insemination examination of the procedure – including doctors’ practices of making social decisions about access...

Ngày tải lên: 01/11/2013, 08:20

Ngày tải lên: 16/01/2014, 16:33

... control of gene expression. As each step of the RNA metabolism is tightly regulated, the regulation of mRNA export, stability and transla- tion rate is essential for the control of the expression of ... activity of both RRMs. Finally, the association of ASF ⁄ SF2 with TIAR led us to investigate its role on the expres- sion of reporter gene bearing an ARE in its 3¢ UTR. We showed that overexpression of ... buffer. After 2 h of binding at 4 °C, beads were washed once with 1 mL of CBB and once with modified CBB (0.02% NP40). Elution of the bound proteins was performed by addition of 5 · 600 lL of calmodulin...

Ngày tải lên: 16/02/2014, 15:20

... of practice. One visible sign of these developments has been the proliferation of codes of ethics or of professional conduct. The drafting of such a code provides an opportunity for professionals to examine ... Australia. Professional Ethics General editors: Andrew Belsey, University of Wales, Cardiff and Ruth Chadwick, Centre for Professional Ethics, University of Central Lancashire Professionalism ... nature of a profession, the function and value of codes of ethics, and the demands of confidentiality. The subject matter of this volume, bankruptcy, raises issues common to a number of fields....

Ngày tải lên: 18/02/2014, 14:20