ethanol and formic acid reaction
Tài liệu Báo cáo " Obtaining Essential Oil and Shikimic Acid from Star Anise Fruit (Illicium verum Hook) " ppt
... oil and shikimic acid from star anise fruits. With this method, obtaining yield of essential oil and shikimic acid respectively are 9.5% and 5.6% (essential oil content and shikimic acid ... Sciences and Technology 26 (2010) 110-113 113 for obtaining simultaneously essential oil and shikimic acid from star anise fruits. The yield of essential oil and shikimic acid were 9.5% and ... as well as shikimic acid obtained by methods N0 1, N0 2 and N0 3 were shown at table 1 and table 2. Table 1. Obtaining simultaneously essential oil and shikimic acid from star anise...
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Tài liệu Báo cáo khóa học: Active-site residues and amino acid specificity of the bacterial 4¢-phosphopantothenoylcysteine synthetase CoaB pptx
... before as important step in the biosynthesis of thelantibiotics epidermin and mersacidin catalyzed by theLanD enzymes EpiD and MrsD, respectively [8–10]. Flavin-dependent oxidative decarboxylation ... N210K and copurification of the 4¢-phosphopantothenoyl-CMP intermediate. His-CoaB proteins(His-CoaB and His-CoaB N210K) were puri-fied from E. coli cell extracts by Ni-nitrilotri-acetic acid and ... Escherichia coli and most eubacteria, the synthesis of4¢-phosphopantetheine, which is also the key reaction incoenzyme A biosynthesis, is catalyzed from 4¢-phospho-pantothenate and cysteine by...
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Tài liệu Báo cáo khoa học: The SCO2299 gene from Streptomyces coelicolor A3(2) encodes a bifunctional enzyme consisting of an RNase H domain and an acid phosphatase domain pdf
... PIPES as an internal standard, and 2 lgofprotein samples in a final volume of 100 lL. The reaction mixture was incubated for 10 min at 37 °C and transferredto 400 lL cold methanol. The contents ... plates containing 50 lgÆ mL)1ampicillin and 30 lgÆmL)1chloramphenicol, and incubatedat 30 °C and 42 °C.Plasmid constructions, overproductions and purificationsPlasmids for overexpression ... RNaseH domain and an APase domain. The RNase H and APase activities of the full length SCO2299 proteindepend on its N-terminal RNase H domain and C-ter-minal APase domain, respectively, and do not...
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Tài liệu Báo cáo khoa học: The swinging movement of the distal histidine residue and the autoxidation reaction for midge larval hemoglobins docx
... ligand. The correspondingspectral changes in the Soret band were therefore followedby stopped-flow and rapid-scan techniques, and theobserved first-order rate constants of kout¼ 25 s)1 and kin¼ ... resistance to acidic autoxidation, we concludethat T. akamusi hemoglobin VII, the major component, canplay an important role in O2transport and storage againstthe extremely acidic and hypoxic ... door for ligandentry. J. Mol. Biol. 207, 459–463.23. Tian, W.D., Sage, J.T. & Champion, P.M. (1993) Investigations ofligand association and dissociation rates in the ÔopenÕ and ÔclosedÕstates...
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Tài liệu Báo cáo Y học: The b-1,4-endogalactanase A gene from Aspergillus niger is specifically induced on arabinose and galacturonic acid and plays an important role in the degradation of pectic hairy regions pdf
... amino acid sequence of GALA revealed veryhigh similarity to the endogalactanases of A. tubingensis(95.7% amino acid sequence identity) and A. aculeatus(78.9% amino acid sequence identity) and ... minD-galactose,D-galactobiose and D-galactotetraose were used as standards to identify theD-galactose and D-galacto-oligosaccharides. The calculatedareas forD-galactose and D-galacto-oligosaccharides ... endogalactanases (between 21% and 28%amino acid sequence identity). This is illustrated by aCLUSTALW analysis [29] of the deduced amino acid sequences of GALA and b-1,4-endogalactanases ofAspergillus...
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Tài liệu Báo cáo Y học: Bivalent cations and amino-acid composition contribute to the thermostability of Bacillus licheniformis xylose isomerase doc
... unchargedpolar amino acids (i.e. Q, N, S, and T) and an increase incharged amino -acid residues (i.e. K, E, and R) inhyperthermophilic proteins. As S and T can catalyze thedeamination and backbone ... The amounts of fructose and xylulose produced weredetermined using the cysteine/carbazole/sulfuric acid method. Absorbance was measured at 537 nm and 560 nmfor xylulose and fructose, respectively. ... 0.981, is based on theelution data of the 200, 150, and 66 kDa protein standards. Linearregressions (1) and (2) give molecular masses of 200 and 177.5 kDa,respectively, for BLXI.6294 C. Vieille...
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A COMPARATIVE STUDY OF THE DIAGNOSIS OF PULMONARY TUBERCULOSIS USING CONVENTIONAL TOOLS AND POLYMERASE CHAIN REACTION* pot
... complex16.Amplification reaction was typically performed ina 50µl reaction mix containing 0.25nmoles offorward and reverse primers, 2mM finalconcentration of dNTP, 1.5 U of Taq polymerasein 1X buffer and proteinase ... These cases were as follows, two eachof asthma, pneumonia and cancer lung, one each ofpost CABG, COPD and lung abscess and 12 withcough and cold. All samples from these individualshad tested ... routinediagnostics like microscopy and cultureTable 3 presents the comparative efficiencyof PCR to diagnostics like microscopy and culture.The sensitivity, specificity, positive and negativepredictive...
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Báo cáo khoa học: Involvement of NF-jB subunit p65 and retinoic acid receptors, RARa and RXRa, in transcriptional regulation of the human GnRH II gene pot
... Differential effects of ligand-activated RARa and RXRa onthe promoter activity of the hGnRH II gene in TE671 and JEG-3cells. In vivo effect of RARa and RXRa with their ligands, ATRA and 9-cisRA, on the ... TE671medulloblastoma and JEG-3 placenta choriocarcinoma cells. Supershift and chromatin immunoprecipitation assays showed in vitro and in vivobinding of NF-jB subunit p65 and the retinoic acid receptors, RARa and RXRa, ... PCR.Treatment of cells with the RARa ⁄ RXRa ligands, all-trans retinoic acid and 9-cis-retinoic acid, reduced and increased GnRH II gene expression inTE671 and JEG-3 cells, respectively. Taken together,...
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ethanol and ozone sensing characteristics of wo3 based sensors activated by au and pd
... WO3gas sensors and multisensors and selectivity enhancement strate-gies including PCA analysis and nose spectroscopy; and modelling of sensorresponses.342 A. Labidi et al. / Sensors and Actuators ... metals. For that, two metals have beenchosen Pd and Au, and their effect on the sensor sensitivitytowards ozone and ethanol was studied. The DC transient and AC responses were analyzed in order to ... Au/WO3 and (b) Pd/WO3.A. Labidi et al. / Sensors and Actuators B 120 (2006) 338–345 343Table 2Modeled RC for WO3bare, Au/WO3 and Pd/WO3based sensors towards 2% of C2H6O and dry...
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low - level detection of ethanol and h2s with temperature - modulated wo3 nanoparticle gas sensors
... dynamic response were ex-tracted by FFT and DWT decomposition methods, and dis-crimination between ethanol and H2S measurements wasperformed by PCA and DFA. DWT was found to outperformFFT ... between 150 and 250◦C for all further measure-ments.3.2. Calibration curves for low concentrations of ethanol and H2SAt first, different concentrations of ethanol (2, 5, 10, 20,30, 40, and 50ppm) ... factorsas to minimise the variance within each class and maximisethe variance between classes [37].The two predefined classes were ethanol and H2S, and the data matrices were the same as the ones...
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Women and Adverse Drug Reactions Reporting in the Canadian Context pot
... or “partner” in meeting the HPB mandate; and • a lack of new criteria for “safety”, new methods for post marketing evaluation of drugs and devices and social and ethical evaluation of new initiatives. ... drug reactions to patients and health-care providers. The jury’s first recommendation called on Health Canada to set up a joint body to review, improve and standardise all communication tools and ... Abraham and Paul Taylor, “Drug reactions kill thousands: researchers”, The Globe and Mail, April 15, 1998. 46 Namrata Bains, MSc; Duncan Hunter, PhD, Adverse reporting on adverse reactions,...
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Báo cáo khoa học: Structural characterization of N-linked oligosaccharides of laminin from rat kidney: changes during diabetes and modulation by dietary fiber and butyric acid pdf
... neutral sugars like mannose and galactose and amino sugar as N-acetylglucos-amine, along with sialic acid which accounted for 22 and 35% of total carbohydrate content in SFC and SFD groups, respectively.Purification ... into SFC and FFC, SFD, FFD and FFD-500 groups. SFC and SFD groups received AIN-76basal diet, whereas FFC and FFD groups received AIN-76diet, wherein starch was replaced with 5% wheat bran and 2.5% ... co-distilledwith distilled water and reduced using sodium borohydride.Excess sodium borohydride was destroyed by adding 2 macetic acid and again repeatedly co-distilled with methanol and dried in a dessicator....
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Báo cáo khoa học: Glycan profiling of urine, amniotic fluid and ascitic fluid from galactosialidosis patients reveals novel oligosaccharides with reducing end hexose and aldohexonic acid residues ppt
... theexception of U4 (Table 3). In U1, U6 and U7, gluconic acid (o1) has high abundance, and high levels of C1-oxi-dized lactose (o2) were observed in urine samples U2,U5 and U6.DiscussionUsing a prototype ... Neu5Ac and the adjacenthexose. No relevant ring fragments were observedbetween C2 and C3, which is consistent with a 1–3linkage between Hex and HexNAc. The ring fragmentions0,2A4 and 2,4A4, ... fragmentions0,2A4 and 2,4A4, and the lack of0,3A4, are indica-tive of a 1–4 linkage between HexNAc and the adja-cent hexose. The ring fragment ions0,2A5,0,2A5-18 and 2,4A5, and the lack of0,3A5,...
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