estrogen modulates oxytocin gene expression in regions of the rat supraoptic and paraventricular nuclei that contain estrogen receptor β
Tài liệu Báo cáo khoa học: Modulation of cyclin D1 and early growth response factor-1 gene expression in interleukin-1b-treated rat smooth muscle cells by n-6 and n-3 polyunsaturated fatty acids pdf
Ngày tải lên :
19/02/2014, 16:20
... blotting) the concentrations of the caveolin-1 and caveolin-3 proteins in the caveolae of cells stimulated with IL-1b in order to determine whether caveolin synthesis is also modulated by PUFA incorporation ... decrease the IL-1b-induced binding of these factors In addition, the expression of genes, associated with atherosclerosis, that contain overlapping GC-box expression are controlled at the transcriptional ... previously that the incorporation of n-3 PUFAs into the SMC membrane modulates various steps of the in ammatory process induced by IL-1b [18] Several lines of evidence suggest that the change in membrane...
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Báo cáo khoa học: Efficient inhibition of b-secretase gene expression in HEK293 cells by tRNAVal-driven and CTE-helicase associated hammerhead ribozymes doc
Ngày tải lên :
08/03/2014, 08:20
... the level of BACE protein, and in consequence, to reduce the amount of Ab peptide released in neuronal cells We asked the question whether we can inhibit expression of the gene of BACE protein ... plasmids Ribozyme encoding plasmids were transiently transfected into the tested cells with the aid of lipofectin Expression of the ribozymes was observed in the course of time The nucleic acid fraction, ... less than 5% of the control BACE mRNA expression in the cells transfected with lipofectin only (NO) and lysed after 48 h increase of the BACE mRNA level because of the lower expression of ribozyme...
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Báo cáo y học: "Analysis of gene expression in operons of Streptomyces coelicolor" ppsx
Ngày tải lên :
14/08/2014, 16:21
... Laing et al shows that the closer gene pairs are together (using adjacent gene pairs on the same strand in the genome), the stronger the correlation in their expression profiles, independent of ... expression _ ratio _ of _ gene _ i _ in _ experiment_j ) Median _ of _ all _ expression _ ratios _ in _ experiment_j We define the expression level of each gene in a given operon of m genes as gi ... downstream of the internal promoter in 57% of cases compared to 49% in equivalent random tests (Figure 5c,d) Internal termination could also play an important role in the control of individual gene expression...
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Báo cáo y học: "Reduced efficacy of selection in regions of the Drosophila genome that lack crossing over" doc
Ngày tải lên :
14/08/2014, 17:22
... the same strand and same contig in the D yakuba genome refereed research We have examined the effect of recombinational environment, in terms of the frequency of crossing over, on the rates of ... 0.35, intermediate 0.35, low 0.39) Again, the GC contents of introns in the non-fourth chromosome genes lie between those of the fourth chromosome genes and the rest of the genome Because short introns ... obtained by bootstrapping by gene/ intron this analysis Interestingly, the mean GC3 value for the no crossover region (0.39) is similar to the GC content of short introns in other recombination regions...
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Báo cáo Y học: A new siglec family member, siglec-10, is expressed in cells of the immune system and has signaling properties similar to CD33 docx
Ngày tải lên :
24/03/2014, 04:21
... capable of binding to the cytoplasmic domain of the siglec-10 protein The binding of SHP-1, however, was missing from the Y667F mutant indicating this to be the preferred tyrosine for interaction ... binding, however, was only diminished by < 50% in the Y667F mutant, indicating that SHP-2 may be binding both to the tyrosine at position 667 and to other tyrosines in the cytoplasmic tail of ... binding of any of the adherent cell types Binding assays with siglec-expressing COS7 cells Results for COS7 binding experiments are shown in Fig 5C There was a significant increase in binding of...
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Báo cáo khoa học: " Degrading of seed quality following 8 months in storage of the rice, maize and soybean basic grade seed" docx
Ngày tải lên :
06/08/2014, 19:20
... methods of testing The fig is illustrated that for soybean seeds as using the sand substrate the percentage of germination is 97.33%, using petri dishes the rate is 85.33% in first month storage The ... rice and maize seed testing and sand substrate is suited for soybean seed testing Table Germination rates (%) of rice, maize and soybean seed following months in storage by petri, sandy substrate ... results of germination rate were different when using was different testing methods and substrates with the same kind of basic seed Table shows that in the first month, rice seeds have germination...
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Báo cáo toán học: "Iodine Alters Gene Expression in the MCF7 Breast Cancer Cell Line: Evidence for an Anti-Estrogen Effect of Iodine" doc
Ngày tải lên :
08/08/2014, 17:20
... changes in the estrogen receptor have been found in the breasts of iodine deficient rats compared to normal euthyroid animals suggesting that the iodine pathway may augment the synthesis of the estrogen ... profiling of an estrogen responsive breast cancer cell line demonstrating that the combination of iodine and iodide alters gene expression Among the list of altered genes were several genes documented ... estrogen responsive such as TFF1 and WISP2 Furthermore, the list contained several genes involved in the estrogen response including Phase I estrogen metabolizing enzymes (CYP1A1 and CYP1B) and...
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Curcumin modulates dopaminergic receptor, CREB and phospholipase c gene expression in the cerebral cortex and cerebellum of streptozotocin induced diabetic rats potx
Ngày tải lên :
10/08/2014, 05:21
... hrs after the administration of morning dose of insulin and curcumin The results were expressed in terms of milligram per decilitre of blood Total Dopamine receptor binding studies in the cerebellum ... interest in learning dopamine D2 receptor expression begins with the hypothesis that dopamine D2 receptors are involved in the pathophysiology of schizophrenia and in the mechanism of antipsychotic ... to determine the fold change in expression This was done by normalizing the resulting threshold cycle (CT) values of the target mRNAs to the CT values of the internal control β- actin in the same...
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Ambient particulate air pollution induces oxidative stress and alterations of mitochondria and gene expression in brown and white adipose tissues ppt
Ngày tải lên :
06/03/2014, 19:20
... for studies of hyperlipidemia and atherosclerosis, and has been used extensively in understanding the mechanisms of lipoprotein metabolism and atherosclerosis The ApoE-/- mice are generated on a ... targeted disruption of the Dio2 gene have cold-induced overexpression of the uncoupling protein gene but fail to increase brown adipose tissue lipogenesis and adaptive thermogenesis Diabetes 2004, ... heat instead of ATP synthesis [23,24] As shown in Figure 6, consistent with the fact that PGC-1a induces mitochondrial biogenesis and thermogenesis [25], its gene expression was marked in BAT...
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Báo cáo khoa học: Diverging regulation of pyruvate dehydrogenase kinase isoform gene expression in cultured human muscle cells pot
Ngày tải lên :
07/03/2014, 17:20
... Collectively, these investigations suggest that changes in the concentration of free fatty acids and insulin are important in regulating the expression of PDK isoforms, either directly or indirectly Alterations ... by the main fuel sources in muscle and by insulin through directly altering the expression of the human PDK2 and PDK4 isoforms In addition to our findings, insulin has been shown to decrease the ... regulating PDK mRNA expression was investigated by incubating myoblasts for h in the presence of insulin (1 lm) and U0126 (100 lm) U0126 failed to reverse the effects of insulin on PDK2 and PDK4...
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Báo cáo khoa học: Oxygen control of nif gene expression in Klebsiella pneumoniae depends on NifL reduction at the cytoplasmic membrane by electrons derived from the reduced quinone pool doc
Ngày tải lên :
17/03/2014, 10:20
... respiratory chain As the most hydrophobic regions of NifL-protein are located in the N-terminal domain [31] that binds the FAD-cofactor [17], one can speculate that the N-terminal domain of NifL ... in the absence of nitrate It appears that this quinol/quinone ratio does not allow NifL reduction by the quinone pool, resulting in high amounts of oxidized cytoplasmic NifL and thus in the inhibition ... regulation of the nuo-operon in K pneumoniae M5a1 Based on preliminary sequence analysis of the promoter upstream regions of the K pneumoniae nuoA gene and determination of the NADH oxidation rate in the...
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Báo cáo khoa học: Effects of juvenile hormone on 20-hydroxyecdysone-inducible EcR, HR3, E75 gene expression in imaginal wing cells of Plodia interpunctella lepidoptera ppt
Ngày tải lên :
23/03/2014, 13:20
... was that the observed declines in the level of PIEcR-B1 mRNA did not occur in the presence of ANS, suggesting that a 20E-induced protein(s) synthesis was involved in these decreases Regulation of ... revealed the expression of ecdysone receptor B1 isoform in imaginal wing cells of P interpunctella at the last larval instar In the fifth larval instar of M sexta, it has been reported in vitro that ... margin designate domains The DNA binding domain (C region) is underlined and the ligand binding domain (E region) is underlined with dashes The helix–turn–zipper motif is double-underlined and...
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Báo cáo khoa học: Expression profile of PIN, AUX ⁄ LAX and PGP auxin transporter gene families in Sorghum bicolor under phytohormone and abiotic stress pot
Ngày tải lên :
29/03/2014, 09:20
... tandem genes (SbPGP19–20 and SbPGP23–24), and another two clusters containing three tandem genes (SbPGP5–7 and SbPGP10–12) Analysis of gene structure SbPIN The PIN gene family is only found in ... was also seen in the SbPIN and SbLAX genes (Fig 6C–F), suggesting that the function of the auxin transport genes might also be involved in the abiotic stresses of salt and drought, and respond ... nucleotide-binding domain in the C-terminus of SbPGP10 proteins is absent Exon–intron structure analysis In addition to phylogenetic analysis, the exon–intron structures of SbPIN, SbLAX and SbPGP genes...
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Báo cáo khoa học: Effect of ecdysone receptor gene switch ligands on endogenous gene expression in 293 cells docx
Ngày tải lên :
30/03/2014, 03:20
... therapeutic indices, such as cytokinins and hormones The main purpose of a regulated gene expression system is to control the timing and levels of transgene expression in vivo Whether the expressed ... specifically in sperm motility and is not involved in the movement of cilia In summary, the use of the THQ gene switch ligand RG-120499 may cause significant changes in the expression of host genes AMK, ... cause loss of DNA repair, which, in turn, is passed on to the next generations, thereby accumulating DNA defects RG-120499 modulates the expression of other genes as well The PNAS123 gene (transformation...
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báo cáo hóa học:" Site-specific analysis of gene expression in early osteoarthritis using the Pond-Nuki model in dogs" pot
Ngày tải lên :
20/06/2014, 00:20
... plateau, and cut to match the surface area of the condyle The areas of India ink staining were outlined using a permanent marker Tracings of the India ink-stained tibial and femoral condyles were ... level of expression for each gene (± standard error) compared to the house keeping gene GAPDH The increase in ADAMTS gene expression in the CrMFC approached significance and was included in the ... assessments The lamina splendens was not disrupted in any location based on lack of India ink staining, indicat- ing that AC surface integrity was maintained for two weeks in the dogs in this study...
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Báo cáo toán học: "A calmodulin inhibitor, W-7 influences the effect of cyclic adenosine 3'''', 5''''-monophosphate signaling on ligninolytic enzyme gene expression in Phanerochaete chrysosporium" pot
Ngày tải lên :
20/06/2014, 20:20
... ligninolytic system in white-rot fungi could be used as a target for breeding a high lignin-degrading strain and for furthering our understanding of the lignin-degradation system in these fungi Phanerochaete ... LiP and MnP are thought to play an important role in initiating the lignin degrading reaction of the fungus, because they can cleave lignin structures extracellularly in the first step of lignin ... and details of the mechanism, including the effect on LiP and MnP transcriptions and the relationship between cAMP signaling and other signal transduction factors, have yet to be determined In...
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Báo cáo toán học: " Effects of internal low-dose irradiation from 131 I on gene expression in normal tissues in Balb/c mice" ppt
Ngày tải lên :
20/06/2014, 21:20
... Seven genes (Dao1 in the kidney cortex and medulla, Asprv1 and Ltf in the lung, Cfd and Lcn2 in the spleen, and Cyba and Cyb5r3 in the liver) were selected from the gene list of significantly ... changes in gene expression after i.v injection of 13, 130, or 260 kBq The total number of transcripts regulated in the tissues investigated is given together with the number of up- (arrows pointing ... Scgb3a1, and Plunc genes in the lungs and the Clec2d, Wsb1, Mup4, Acaa2, and Mpo genes in the liver showed a monotone increase or decrease in expression with the absorbed dose An example of more...
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báo cáo hóa học:" A calmodulin inhibitor, W-7 influences the effect of cyclic adenosine 3’, 5’-monophosphate signaling on ligninolytic enzyme gene expression in Phanerochaete chrysosporium" pot
Ngày tải lên :
21/06/2014, 17:20
... important role in initiating the lignin degrading reaction of the fungus, because they can cleave lignin structures extracellularly in the first step of lignin mineralization (Cullen and Kersten ... demonstrate that cAMP and IBMX increase the transcription levels of most LiP and MnP isozyme genes We also investigated the relationship between the cAMP pathway and calmodulin (CaM), which is the ... MnP expression that can be blocked by CaM inhibition Transcriptions of the isozyme genes following exposure to the stimuli The genome of P chrysosporium RP78 is predicted to contain 10 and genes...
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Báo cáo hóa học: "A calmodulin inhibitor, W-7 influences the effect of cyclic adenosine 3’, 5’-monophosphate signaling on ligninolytic enzyme gene expression in Phanerochaete chrysosporium" pptx
Ngày tải lên :
21/06/2014, 19:20
... important role in initiating the lignin degrading reaction of the fungus, because they can cleave lignin structures extracellularly in the first step of lignin mineralization (Cullen and Kersten ... demonstrate that cAMP and IBMX increase the transcription levels of most LiP and MnP isozyme genes We also investigated the relationship between the cAMP pathway and calmodulin (CaM), which is the ... MnP expression that can be blocked by CaM inhibition Transcriptions of the isozyme genes following exposure to the stimuli The genome of P chrysosporium RP78 is predicted to contain 10 and genes...
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Báo cáo sinh học: "Conservation of core gene expression in vertebrate tissues" pdf
Ngày tải lên :
06/08/2014, 19:20
... species TF genes within the set of unique orthologs were defined by the presence of a DNA-binding domain in the mouse protein sequence in the Pfam database [75] Our list of TF genes is given in Additional ... These and other techniques require experimental data for training and testing, and we now provide such data for tens of thousands of genes across the vertebrate lineage, including several thousand ... events in each gene and tissue using the binary measure We split each set of orthologs in each tissue in each species on the basis of their measured normalized expression intensities according to the...
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