essential role of dna repair proteins at the telomere

... progression The finding that both TRIP-Br proteins interact with cyclin A further strengthens the hypothesis that the TRIP-Br proteins are putative regulators of the cell cycle It is conceivable that the ... in the context of E2F-1/DP-1 regulation, co-expression of KRIP-1 with the TRIP-Br proteins further augments the co-activation of E2F-1/DP-1 by the TRIP-Br protein 26 These data suggest that the ... Given that the E2F transcription factors promote the G1-to-S transition and that E2F-1 plays a role in the regulation of cell proliferation, the unique ability of the TRIP-Br proteins and the PHD-bromodomain...

Ngày tải lên: 16/09/2015, 17:12

... distinct mechanisms of activation of cryptic mitochondria-targeting signals at the N-terminus of mammalian CYP proteins [4,10] We found that post-translational processing of CYP1A1 at the 4th and 32nd ... concentration (0.2 m NaCl) to be released from the membrane (data not shown) Role of PKA in the mitochondrial targeting of CYP2E1 The role of PKA in mitochondrial targeting of CYP2E1 was investigated ... volume The reaction mixture was incubated at 37 °C for 30 min, and the reaction was stopped by the addition of 0.1 mL of 25% zinc sulfate and 0.1 mL of saturated barium hydroxide After centrifugation...

Ngày tải lên: 18/02/2014, 16:20

... Owing to the lower steric limitations of the side chain of the Ala than of the Leu, water may occupy the space Furthermore, the side chain of His140, which is coordinated to the T3 copper, rotated ... mutant at least partly loses its chloride ion and probably also the T2 copper, as the B-value of the T2 copper was rather high On the basis of the anomalous signal, the estimated occupancy of the ... of the protein during the post-translational processing, and thereby the function of the mature laccase Our structural analysis of wild-type MaL indicated that the last four amino acids of the...

Ngày tải lên: 16/03/2014, 00:20

... example of the emergence of large consortia of researchers at the expense of the more classical investigator-initiated approach of the past31 To the surprise of many, the complete sequence of the ... age First formulated in the 1950s, the somatic mutation theory explains aging as a gradual accumulation of random alterations in the DNA of the genome in the cells of our body This theory has proved ... together, whereas the farther away they are from each other, the more chance of their INTRODUCTION being separated by the process of crossing over The Morgan is now the unit of measurement of...

Ngày tải lên: 11/06/2014, 10:21

... known to be of Spanish/Basque extraction Oxygenation and deoxygenation of hemoglobin occur at the heme iron The sigmoid shape of Hb-oxygen dissociation curve is indicative of cooperative interaction ... variant) adequate oxygenation of the tissue is reestablished and Epo production plateaus and at this new steady state serum Epo is often at normal level This leads to stabilization of Hb level ... pressure of oxygen (venous pO2), venous pH and venous oxygen saturation, and uses anti-log mathematical function that many clinicians find difficult to use for calculation [5] The P50 of a healthy...

Ngày tải lên: 08/08/2014, 16:23

... cells and clathrin molecules (Additional File 3) Together, these data suggest the involvement of clathrin in the endocytosis of the DENV particles To affirm the role of clathrin-mediated endocytosis ... AP2B1 are the subunits of the clathrin-associated adaptor protein complex that regulate the formation of clathrin-coated pits as well as linking clathrin to cellular receptors in Page of 17 endocytic ... Rab11B (68%) and Rab7 (61%) The clathrin heavy and light chains are intricately braided together to form the triskelion coat that facilitate the formation of clathrin coated pits for endocytosis...

Ngày tải lên: 12/08/2014, 04:21

... that radiation sensitivity may deviate from the LQ model, especially at low doses; mathematical models have been generated to indicate the extent of such deviation [6] Assessing the effect of DNA ... between treated and untreated experiments, the merits of the approach were evaluated in terms of the capacity of the model to quantify and indicate the relative significance of the effects of PARP ... and the significance of terms in the model was tested using the log-likelihood ratio test This test considers the ratio of the likelihood of the model with the parameter to the model without the...

Ngày tải lên: 13/08/2014, 16:21

... pathways and associated diseases DNA methylation has been implicated in gene regulation in multiple physiological systems This dissertation examines the role of DNA methylation in regulating the ... uses the methylation pattern of the original DNA strand as a template for catalyzing a symmetric methylation pattern on a newly synthesized DNA strand (Otto and Walbot, 1990) Because of its role ... but the interactions of MeCP2 with methylated DNA are the main focus of this dissertation The Cedar laboratory conducted one of the first studies linking chromatin structure with DNA methylation...

Ngày tải lên: 24/08/2014, 09:47

... is the measured extension of the nucleoprotein complex, zsaturated is the extension of the nucleoprotein complex obtained at saturation, znaked is the extension of naked DNA in the absence of proteins, ... elucidate the organisation modes of MvaT to DNA using magnetic tweezers, and visualize the conformations of MvaTDNA complexes using atomic force microscopy We show for the first time the existence of ... stretched DNA Note that when the extension of the DNA is ⇠ µm, the image of the bead is near the edge of our observation area and may interfere with the measurement Therefore, we didn’t let the extension...

Ngày tải lên: 09/09/2015, 11:26

... part consists of: the rationale, the presupposition, the aims, and the significance, the scope, the methods, and the design of the thesis Part two is the development, which consists of chapters: ... the absolute possessor of knowledge that he delivers to the learners Students as the teacher says, so that the teacher‟s knowledge can be transmitted to them The role of the learner is that of ... degree of independence in their use of English, one that they have probably not experienced before If the choice of topic, the research, and the preparation become the sole responsibility of the...

Ngày tải lên: 24/09/2015, 15:42

... will further explore the recent theories of leadership, and evaluate if these make a further contribution to the recognition of the skills and attributes of women in management or leadership roles ... perceptions of the appropriateness of women in management and leadership roles, or are the attitudes relating to the appropriateness of women in such roles still influencing organisational decision ... 2004, p 56) Here the focus extends beyond influence to include motivation and enabling of others to help achieve the goals of the organisation Furthermore, the ability to control others is given...

Ngày tải lên: 08/10/2013, 15:56

... constantly Aims of the study The study is aimed at: Investigating the current reality of the teaching and learning of speaking skill at the PPA Identifying the difficulties and obstacles that the teachers ... say that after giving topics, their teachers often give them words and structures needed and 52% state that their teachers often help them with the ideas Those who answer that their teachers often ... teachers at the PPA are well aware of the benefits of CLT, many of them admit that they find it hard to apply CLT into their teaching According to them there are many reasons for this, however, the...

Ngày tải lên: 06/02/2014, 14:40

... teratomas usually contain fat Because DWI with EPI sequences usually uses a fat saturation RF pulse, the low ADC values of the cystic component of mature cystic teratomas have been attributed to artifacts ... fat within the tumor [21, 22] Furthermore, mature cystic teratoma is lined with keratinized squamous epithelium in most cases [23] The restricted Brownian movement of water molecules within the ... decrease in the ADC [21, 22, 28, 49] The mean ADC of mature cystic teratomas was lower than of malignant ovarian cystic tumors (Figs 6, 8) [22,23] The cystic components of mature cystic teratomas...

Ngày tải lên: 13/02/2014, 06:20

... investigate the contribution of the motor neuron to PRiMA-linked G4 AChE at NMJs, we analyzed the effect of denervation on the localization of PRiMA The NMJs of the denervated tibialis and sham-operated ... with the presence of a significant amount of AChE activity in the presynaptic membrane at NMJs of the rat lumbricalis muscle [27] The presence of AChE in the presynaptic membrane can facilitate the ... indicates that PRiMA at NMJs is mainly provided by motor neurons Innervation regulates the expression of PRiMA-linked G4 AChE in the spinal cord The expression of PRiMA and the pattern of AChE molecular...

Ngày tải lên: 18/02/2014, 08:20

... lack of adduct formation, but rather indicates that the binding process is not associated with a measurable net heat change The finding that binding of UDPNAG to the K22V mutant protein restores the ... important residue in the stabilization of the closed conformation of the protein This hypothesis can be tested by assessing the extent of the conformational change occurring upon addition of UDPNAG to ... course of enzymatic enolpyruvyl transfer and catalytic conformation of the active site revealed by the crystal structure of the fluorinated analogue of the reaction tetrahedral intermediate bound...

Ngày tải lên: 19/02/2014, 13:20

... centrifugation at 40 000 g for 30 at °C The pH of the supernatant was adjusted to a value of 8.0, by adding, alternatively, pH-unadjusted stock solutions of Tris and EDTA The final concentration of ... interior of the protein matrix The script a is the peak–peak distance between the maximum at %287 nm and the minimum at %283 nm, and the script b is the peak–peak distance between the maximum at %295 ... Figure shows the dependence of the relative intrinsic fluorescence intensity of PsbQ with the Fig Fluorescence quenching of the native PsbQ protein Stern– Volmer analyses of the quenching of the single...

Ngày tải lên: 21/02/2014, 00:20

... function of , where is the vector of zero-inflated covariates and is the vector of zero-inflated coefficients to be estimated The function F that relates the product (which is a scalar) to the probability ... and asset portfolio rather than solely the poultry income, thereby providing a more accurate measure of the impact of the disease If one looked only at the impact of HPAI on the income from poultry ... and thus facilitate the implementation of control measures, is a function of the effect of HPAI on livelihoods This link rationalizes the system of compensation for the loss of poultry from control...

Ngày tải lên: 21/02/2014, 01:20

... relationship (Fig 3B) The pK2 increased as the size of the residue at position 169 increased The effect of the size of the residue at position 169 may be related to the solvent accessibility of ... peak at 37 °C (Fig 4) The data indicate that the split signals were originated by the one proton, His57 Nd1-Asp113 Oc2, i.e at high temperature, the interchange rate of the proton between His57 ... based on the X-ray crystal structure of wild-type API The coordinates for the API variants were taken from PDB file code 1arb The appropriate residues were changed at the site of the mutation and...

Ngày tải lên: 21/02/2014, 03:20

... immunogenicity of the antibody framework from a different species [23–25] The success of the series of studies shows that the stable framework structure of Fv enables the transfer of function by means of ... 100-fold the affinity of either of the scFvs alone Local library approaches have also been attempted for the design of appropriate polypeptide linkers with a repeat unit of (XGGGS)n, in which the residues ... for grafting They investigated deviations in the amino acid sequences of the CDRs of 1330 human light chains to identify the candidate residues important in the light chain conformation Peptide...

Ngày tải lên: 06/03/2014, 11:20

... condensation of the fatty acid with the N-terminal tryptophan of the nonribosomally synthesized peptide is necessary Here, we report the results of a steady-state kinetic analysis of DptE The aim of ... been shown that the activity of these acidic lipopeptides is significantly influenced by the length and structure of their fatty acid moieties [9,10] In the fermentation of these natural products, ... product formation activity (data not shown) Therefore, the results clearly demonstrate that holo-DptF is the cognate acceptor substrate of DptE (Table 2) Fatty acid specificity of the AMP ligase...

Ngày tải lên: 07/03/2014, 04:20