esi ms and tandem mass spectrometry ms ms

... extraction 40 2.2.6 Lipid analysis by electrospray ionisation mass spectrometry (ESI- MS) and tandem mass spectrometry (MS/ MS) 40 2.2.7 Data processing 42 2.3 Results ... tentatively assigned by their mass- to-charge (m/z) ratio Characterisation of ions can be achieved by collision-induced dissociation (CID) and tandem mass spectrometry (MS/ MS) (B) MS/ MS spectra of ions with ... Non-targeted profiling and characterization of glycerophospholipids and sphingolipids of slc1Δ by ESI- MS, MS/ MS and MS3 67 3.3.3.2 Non-targeted profiling of glycerophospholipids and sphingolipids...

Ngày tải lên: 14/09/2015, 14:06

... expression vector, without (lanes and 3) and with the coding sequence for DmTpc1p (lanes and 4) Samples were taken at the time of induction (lanes and 2) and h later (lanes and 4) The same number of ... DmTpc2p) with a calculated molecular mass of 36.4 kDa (Fig 1) DmTpc1p and DmTpc2p share 39% of identical amino acids They have 33% and 31% sequence identity and 53% and 51% sequence similarity to human ... [9], and then ascertained to be the human Tpc [8] Tpc1p and Tpc belong to the mitochondrial carrier family (MCF) [10– 12] Family members have a tripartite structure consisting of three tandemly...

Ngày tải lên: 29/03/2014, 08:20

... Multi-isotope imaging mass spectrometry (MIMS) has been developed from secondary-ion mass spectrometry (SIMS) by adding sophisticated ion optics, labeling with stable isotopes and quantitative image-analysis ... isobars, such as 12C15N- (mass 27) and 13C14N- (also mass 27) and other similar atomic clusters Slodzian’s instruments enabled both high spatial resolution and the high mass resolution necessary ... community and has a long-standing interest in SIMS instrumental developments Slodzian is an ion physicist of enormous skill and reputation who has been responsible for the ion-optical design of...

Ngày tải lên: 06/08/2014, 18:21

... band and a faint HMW band Fig 11: Exemplary semi-native Western blot of the adiponectin molecular weight forms of dairy cows at different stages of lactation (Day and 105 post partum (p.p.)) and ... of glucose and lipid metabolism (Waki et al., 2003) Contrary to other adipokines produced by AT, e.g Leptin and Visfatin, AdipoQ is inversely correlated with body mass and insulin resistance (Arita ... mean intensities of the bands corresponding to the monomers (28 kDa) and dimers (56 kDa) were totaled for each lane, and then the means of the duplicates from samples and standards were calculated...

Ngày tải lên: 20/11/2015, 15:49

... site-directed mutagenesis of four active site residues of Dm-dNK Residue E52 was mutated to D, H and Q, residue Y70 to W, residue Q81 to N and residue R105 to K and H The kinetic properties of the active ... Hydrogen-bonding residues are shown E52, Y70, Q81 and R105 were mutated in this study Residues V84, M88 and A110, mutated in a previous study [15], are also included 1544 We performed site-directed mutagenesis ... R-factor and Rfree were 23.2 and 24.2%, respectively (Table 3) Most of the protein could be found in the electron-density map, with the exception of residues 1–11 and 210–230 The loop connecting a9 and...

Ngày tải lên: 19/02/2014, 02:20

... scaled and merged using the programs mosflm [30] and scala [31] Data collection statistics are shown in Table Structure determination and refinement Structures with the same space group and similar ... density maps, contoured at 3r, for (A) dC and one sulfate ion and for (B) AZT and two sulfate ions All hydrogen bonds are shown as red dotted lines and water molecules are shown as red balls ... BVDU and brivudine monophosphate (BVDUMP) in the HSV-1-TK + BVDU complex [19] and the VZV + BVDUMP and ADP complex [20] Zidovudine (AZT) is a potent inhibitor of HIV replication in vitro and at...

Ngày tải lên: 16/03/2014, 06:20

... policy’ (Mandela 1993: 93) Both the Mandela and Mbeki administrations drew a one-to-one connection between lack of human rights and democracy, on the one hand, and Africa’s instability and lack ... Quoting Alden and Le Pere (2004: 29), Kagwanja notes that ‘this has forced the Mbeki administration to abandon the unilateralism and moral rectitude that underpinned the Mandela presidency and turn ... INTRODUCTION Senegal and Mauritania, and between Senegal and Guinea-Bissau; crises in Guinea-Conakry and Guinea-Bissau; and internationalised civil wars in Sierra Leone and Liberia ECOWAS, or...

Ngày tải lên: 29/03/2014, 03:20

... postnatal mouse cochlea and vestibular system[12,13], and adult human and guinea pig spiral ganglion[27]; (2) spheres were shown to be clonal and capable of self renewal[12,13]; and (3) spheres were ... RCMN: design of the study, critical review of data and the manuscript, and provider of the laboratory structure and support for the project LAH: technical supervision on cell culturing and immunofluorescence ... cells and supporting cells upon culturing of mouse otospheres under conditions favoring cell differentiation (Figures and 6) All markers employed, myosin VIIa and jagged2 for hair cells and p27kip1...

Ngày tải lên: 18/06/2014, 16:20

... case the individuals are the populations, and the variables are the genotypic and gametic frequencies II Material and methods Two wine cellar populations and two field populations of Drosophila ... chromosome, 38.8 cM) and aldehyde oxidase (Aldox locus, 3rd chromosome, 56.6 cM) The procedures for electrophoresis and staining are described by O’B & MCIN!RE (1969), I!ICKINSON (1970) and P (1957) ... a-Gpdh and Est-6 loci were each polymorphic for two alleles, F and S The Aldox locus was polymorphic for three electromorphs One allele, Aldox‘’ was not S found in the cellar populations and also...

Ngày tải lên: 09/08/2014, 22:22

... continual wars supported themselves, and though they involved the manufacture of arms and munitions, and so furnished employn~entto the industries connected with the equipment and maintenance of armies, ... delicate operation, demanding so much attention, so much foresight, so much care, such minute observation of the methods used by each tradesman, and the adjustment of supply and demand in each locality, ... timidity on this point is that the railroads and telegraph were not yet in existence, and communication between Paris and the provinces was slow and difficult and often uncertain Having made this digression...

Ngày tải lên: 05/11/2014, 08:18

... populations: E1.1 (Rajo, Gialova, Pugnochiuso and Bin El Ouidane), E2.1 and E2.2 (Bin El E1.1 77.0 42.9 4.5 21.6 46.2 Ouidane and Pugnochiuso) and E2.3 (Minorca and Bin E1.2 7.0 26.9 El Ouidane) These ... the Mediterranean area and Saharan Mountains, lineages E2 and E3 were specific to the Western Mediterranean area, lineage M was only detected in Macaronesia, lineages C1 and C2 were observed from ... Science and Innovation GD was supported by projects 041/C/2007, 75/C/2009 & 56/C/ 2010 of “Consejería de Agricultura y Pesca, Junta de Andalucía"; “Grupo PAI” AGR-248 of “Junta de Andalucía"; and...

Ngày tải lên: 11/08/2014, 11:20

... with vascular leakage, and the infiltration of inflammatory cells [3] The pathogenesis of asthma and its susceptibility involve a complex interplay of various genetic and environmental factors, ... digitized, and analyzed using a flat scanner (PowerLook 3000, UMAX, Taipei, Taiwan) and GenePix 3.0 software (Axon, Union City, CA) The replicates were used to calculate the mean and standard deviation ... macrophages by PDE type IV inhibitors and cAMP-elevating agents Two PDE type IV inhibitors, etazolate and rolipram, and two cAMP-elevating agents, forskolin and db-cAMP (dibutyryl-cAMP), were added...

Ngày tải lên: 12/08/2014, 18:22

... autoradiography The left-hand lanes show the molecular mass standards (St) and lg of unphosphorylated calpain B protein (P) stained with Coomassie brilliant blue The molecular mass of the standards is given ... band (–EGF) or a doublet of bands (+EGF) that corresponds to the molecular mass of calpain B These bands were excised, digested with trypsin and analyzed by MS The extracted ion chromatograms ... from N-terminal Gln residues and phosphorylation of Ser, Thr and Tyr residues Data were also manually inspected Calpain B activity measurements MS The active, wild-type and the inactive, mutant...

Ngày tải lên: 16/03/2014, 02:20

... 206 and 280 nm The proctolin fragments Arg-Tyr and Leu-Pro-Thr were identified by coelution with standard solutions of di- and tripeptide The effect of proctolin concentrations (from to 250 lM) and ... Arrows represent the primer pairs (1 and 2; and 4) used in the PCR and RT-PCR experiments, and the approximative sequence area expected to be amplified (1 kb and 0.8 kb, respectively) (B) Transfection ... lines 1a and 1b) after 48 h induction (lanes and 3) or not previously induced (lanes and 4) for the synthesis of D melanogaster DPP III were separated by SDS/PAGE on a 7.5% acrylamide gel and stained...

Ngày tải lên: 17/03/2014, 10:20

... in the repair of MMS- and H2O2-induced DNA lesions Results Molecular cloning of Drosophila PCNA2 The studies on PCNA in archaeans and higher plants suggest that some organisms may have several ... PAGE and blotted with anti-Flag serum The 30 kDa protein band of Flag-tagged DmPCNA2 and the 36 kDa protein band of Flag-tagged DmPCNA1 are indicated by arrows The sizes of the molecular mass ... repair of MMS-, H2O2- and UV lightinduced lesions in a similar manner to other eukaryotic PCNAs In eukaryotes, base excision repair is known to be the major pathway for repair of MMS- and H2O2-induced...

Ngày tải lên: 23/03/2014, 10:20

... 26; black bars) were expanded in primary culture for 10 to 12 days and the gene expression of type I and II collagen (CI and CII, respectively), aggrecan (AGG), IL-1β and activin-like kinase ... engineering company CG and JF have stock holdings in TETEC and have received salaries from the company RS and WKA have received research funding and royalties, respectively The terms of the financial ... by electrophoresis and visualized on agarose gels to confirm the size and purity of the PCR products Amplification of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) encoding cDNA and serial dilutions...

Ngày tải lên: 09/08/2014, 10:20

... background and design of the study and drafted the manuscript ID, SMP, JRE, JWS, and RJW contributed to the background and design of the study SMP developed the statistical approach and conducted ... detection reagents for many polymorphic systems presynthesized and tested, "on demand." Detection reagents for other variants are ordered "on demand" through a user-friendly WWW interface We ... r2 between typed and untyped variants is 0.96 High density SNP mapping can indirectly assay other forms of common genetic polymorphisms, such as repeat length polymorphisms and insertions/deletions...

Ngày tải lên: 11/08/2014, 17:20

... EB and ART carried out the EM analysis FA carried out the confocal analysis BF, AMF and MM participated in the design of the study FG and CV conceived of the study, and participated in its design ... antibodies pAbGagZAM and a rat anti-YL targeting yolkless receptor were then added at 1/100 and 1/200 respectively, and incubated overnight at 4°C Secondary antibodies (goat anti-rabbit Cy3 and goat anti-rat ... design and coordination and helped to draft the manuscript All authors read and approved the final manuscript Acknowledgements We thank Dr S Frankenberg for comments on the manuscript, and Dr...

Ngày tải lên: 13/08/2014, 09:21

... merely an issue of supply and demand, as the relevance lies more within the process of accessing, engaging and mobilizing water in tandem with the larger social, economic and political dynamics ... disciplined and educated population and the services and amenities that were already in place for Singapore’s entrepot trade.18 In tandem with the claim of the need for a disciplined and educated ... per thousand gallons to $4.00 per thousand gallons for shipping; $0.60 per thousand gallons to $0.80 per thousand gallons for domestic; $2.00 per thousand gallons to $2.50 per thousand gallons...

Ngày tải lên: 03/10/2015, 20:30