erk1 2 map kinase pathway in cancer

... the cell by a series of protein-protein interactions such as insulin receptor substrates and (IRS-1 and IRS -2) , phosphatidyl- inositol-3 (PI3) -kinase, and protein kinase (PK) B/Akt Activation ... induced by insulin [15] Hernandez et al have suggested that ERK and p38 MAPK could be major factors in TNF-α-induced insulin resistance in brown adipocytes [16] Those findings indicate that ERK pathway ... in adipocyte physiology other than inflammatory cell recruitment In conclusion, our data suggest that IL-8 is a main adipocytokine producing insulin resistance via the inhibition of insulin-induced...

Ngày tải lên: 11/08/2014, 08:22

... generates binding sites for proteins containing Src homology -2 (SH2) and protein tyrosine-binding (PTB) domains4 Two families of tyrosine kinases exist within cells They are the receptor tyrosine kinases ... transcription TATA-binding protein Tris buffered saline-Tween Tyrosine kinase inhibitors U2 auxiliary factor Non-deletion minigene Zinc-finger nucleases xiii Chapter Introduction 1.1 TYROSINE KINASES AND ... Chapter Introduction……………………………………………………………… 1.1 Tyrosine kinases and their signaling pathways…………………………… 1 .2 The role of tyrosine kinases in cancer …………………………………… 1.3 Tyrosine kinase inhibitors...

Ngày tải lên: 10/09/2015, 09:04

... 44 23 ( 52. 3) 21 (47.7) > 60 years 40 22 (55.0) 18 (45.0) 38 46 21 (55.3) 24 ( 52. 2) 17 (44.7) 22 (47.8) 0. 828 Well and moderate 40 20 (50.0) 20 (50.0) 0.6 62 Poor 44 25 (56.8) 19 (43 .2) I 44 21 ... Fukuda R, Kinoshita Y: Activation of prostaglandin E2-receptor EP2 and EP4 pathways induced growth inhibition in human gastric carcinoma cell lines J Lab Clin Med 20 02, 140: 92- 1 02 Dubinett SM, ... changes in COX -2, downstream PGE2, and protein kinase signal transduction pathways have been demonstrated in some tumors [28 ,29 ] PGE2 binds to four subtypes of G-protein-coupled receptors–EP1, EP2,...

Ngày tải lên: 10/08/2014, 10:20

... beta-cells J Mol Biol 324 , 27 1 28 5 16 Zhang SP, Liu JX, Saafi EL & Cooper GJS (1999) Induction of apoptosis by human amylin in RINm5F 3790 17 18 19 20 21 22 23 24 25 26 27 28 29 islet beta-cells ... mitogen activating protein (MAP) kinase (p38 kinase) followed by caspase-3 activation [24 ] Mitogen-activated protein kinases (MAPKs) are a group of protein serine ⁄ threonine kinases that play ... Metab 89, 3 629 – 3643 Gueli N, Toto A, Palmieri G, Carmenini G, Delpino A & Ferrini U (1987) In vitro growth of a cell line originated from a human insulinoma J Exp Clin Cancer Res 6, 28 1 28 5 Baroni...

Ngày tải lên: 07/03/2014, 12:20

... 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 Tan PL, Farmiloe S, Yeoman S, Watson JD: Expression of the interleukin gene in rheumatoid synovial fibroblasts J Rheumatol 1990, 17:1608-16 12 ... effectall-trans retinoic acid (ATRA) of interleukin-1-beta (IL-1β) in the presence or absence of the ERK1/ 2 inhibitor PD-98059 or ATRA (a) Western blotting of phosphorylated ERK1/ 2 (P -ERK1/ 2) after 15 minutes ... control are indicated as *P < 0.05 and from IL-1β-stimulated cells as #P < 0.05 ERK1/ 2, extracellularly regulated kinase 1 /2; JNK, c-Jun N-terminal kinase; MAPK, mitogen-activated protein kinase; ...

Ngày tải lên: 09/08/2014, 01:22

... that MAPK pathways might be involved in IL-1β-induced SYN expression To further elucidate which MAPK pathway is involved in regulating IL-1β-induced SYN expression, specific inhibitors of MAP kinases ... Res Ther 20 05, 7:R1140-R1147 Hammaker DR, Boyle DL, Chabaud-Riou M, Firestein GS: Regulation of c-Jun N-terminal kinase by MEKK -2 and mitogen-activated protein kinase kinase kinases in rheumatoid ... inhibitors used in this study, including the Erk activation inhibitor, PD98059 [13], the c-Jun N-terminal kinase (JNK) inhibitor, SP600 125 [14], p38 inhibitor, SB2 021 90 [15], and the NFκB inhibitor,...

Ngày tải lên: 09/08/2014, 08:23

... 20 10, 17:15 http://www.jbiomedsci.com/content/17/1/15 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 receptor 4-deficient mice following pressure overload Cardiovasc Res 20 05, 68 :22 4 -23 4 ... potent inhibitors of an upstream p38 kinase, SB203580 inhibited the induction of TLR4 protein This signaling pathway of p38 was further confirmed by the finding that p38 siRNA inhibited the induction ... DNA-protein binding activity induced by stretch (Fig 6A) Our finding indicates that Ang II receptor-related mechanism is involved in cyclic stretch-induced NF-B activity DNA-binding complexes induced...

Ngày tải lên: 10/08/2014, 05:21

... protein kinase (MAPK) kinase (MEK1 /2) by phosphorylation of Ser217/ Ser 221 [19] Subsequently, MEK1 /2 activates extracellular receptor kinase 1 /2 (ERK1/ 2) by phosphorylating Thr2 02/ Tyr204 ERK1/ 2 ... IL-1: interleukin-1; ILK: integrin-linked kinase; KD: kinase- deficient; MAPK: mitogen-activated protein kinase; MEK1 /2: mitogen-activated protein kinase/ extracellular receptor kinase 1 /2; MTT: ... analysis showing ERK1/ phosphorylation using phospho-Thr2 02/ Tyr204 ERK1/ 2 (P -ERK1/ 2) and total ERK1/ 2 (T -ERK1/ 2) antibodies (f) Immunofluorescence analysis showing minimal phospho -ERK1/ 2 in control...

Ngày tải lên: 12/08/2014, 14:21

... 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 1 12 Hunter, T Protein kinases and phosphatases: The yin and yang of protein phosphorylation and signalling Cell, 1995 80: 22 5 -23 6 Schenk, ... ATP binding site serves as a docking site for specific binding of cytoplasmic signaling proteins containing Src homology -2 (SH2) and protein tyrosine binding (PTB) domains These proteins in turn ... tyrosine kinase inhibitors Inhibiting the activity of tyrosine kinases by low molecular weight compounds capable of interfering with either ligand binding (in the case of receptor tyrosine kinases)...

Ngày tải lên: 03/11/2012, 09:57

... very-KIND -MAP2 interaction v-KIND KIND2 KIND2-1 KIND2 -2 KIND2-3 KIND2-4 KIND2-5 A 620 521 555 488 589 EGFP EGFP-KIND2-1 EGFP-KIND2 -2 EGFP-KIND2-3 EGFP-KIND2-4 EGFP-KIND2-5 EGFP-KIND2 EGFP-KIND1 ... v-KIND KIND2 domain contain the MAP2 -binding core site (A) Division of the KIND2 domain (456– 620 ) into five subregions: KIND2-1 (amino acids 456–487), KIND2 -2 (amino acids 456– 521 ), KIND2-3 (amino ... 667 CD2-1-3 701 CD2-1-4 CD2-1-5 735 CD2-1-6 7 02 CD2-1-7 668 CD2-1-8 635 CD2-1-6-1 7 02 CD2-1-6 -2 CD2-1-6-3 Interaction PD Input GST CD2-1-1 CD2-1 -2 CD2-1-3 CD2-1-4 CD2-1 767 25 0 150 PD 734 Input...

Ngày tải lên: 14/02/2014, 19:20

... experiments Control +S1P +Anisomycin Y -27 6 32 ) ) + – + – + G-Actin/F-Actin 0 .26 0 ± 0. 023 0.181 ± 0. 023 * 0 .22 ± 0. 021 0 .23 9 ± 0.018 0 .22 3 ± 0.013 0.110 ± 0.034** 0 .22 7 ± 0.019  *P < 0.05, **P < 0.01 ... to F-actin ratio, suggesting that RhoA/actin-independent signaling mechanisms are involved in anisomycin-induced CTGF/ CCN2 expression CTGF /CCN2 gene regulation through MAP kinase signaling Because ... Allen, T.J (20 02) Prevention of accelerated atherosclerosis by 18 19 20 21 22 23 24 angiotensin-converting enzyme inhibition in diabetic apolipoprotein E-deficient mice Circulation 106, 24 6 25 3 Chaqour,...

Ngày tải lên: 19/02/2014, 16:20

... YHUM214 YHUM216 MR161 MR211 MR298 AR2 MR287 AR3 AR4 Source CEN.PK2; MATa ras2::kanMX ura3– 52 leu2–3, 1 12 his3-D1 trp1 28 9 MAL2–8c SUC2 CEN.PK2; MATa gpa2::TRP1 ras2::kanMX ura3– 52 leu2–3, 1 12 his3-D1 ... trp1 28 9 MAL2–8c SUC2 [p 426 MET25-RAS2] CEN.PK2; MATa ras1::S.pombe his5+ ras2::kanMX ura3– 52 leu2–3, 1 12 his3-D1 trp1 28 9 MAL2–8c SUC2 [pFL38-RAS2] S 127 8b; MATa ura3– 52 his3:hisG leu2::hisG sut2:: ... leu2::hisG ras2::kanMX S 127 8b; MATa/a ras2::kanMX/ras2::kanMX ura3– 52/ ura3– 52 his3:hisG/his3:hisG leu2::hisG leu )2: :hisG S 127 8b; MATa/a sut2::S.pombe his5+/sut2::S.pombe his5+ ura3– 52/ ura3– 52 his3:hisG/his3:hisG...

Ngày tải lên: 07/03/2014, 15:20

... scaffolding proteins, which promote interaction between the links of the pathway For instance, MP1 can bind MEK and ERK1 supporting ERK1 activation [26 ], and kinase suppressor of RAS can bind RAF, ... kinases Oncogene 14, 1635–16 42 21 Bapat, S., Verkleij, A & Post, J.A (20 01) Peroxynitrite activates mitogen-activated protein kinase (MAPK) via a MEKindependent pathway: a role for protein kinase ... was unable to induce pleckstrin _ Ro 31 822 0 _ + _ + 25 kDa WB: P-ERK Cvx Cvx +Ro kDa Thr 25 kDa _ Ro 31 822 0 _ + _ + Pull-down: RAF-RBD 25 kDa WB: RAS Fig PKC inhibitor Ro-31 822 0 inhibited ERK...

Ngày tải lên: 08/03/2014, 16:20

... (20 01) Induction of BIM, a FEBS Journal 27 6 (20 09) 326 9– 327 6 ª 20 09 The Authors Journal compilation ª 20 09 FEBS 327 5 NGF induces miR -22 1 and 22 2 expression 18 19 20 21 22 23 24 25 26 27 28 K Terasawa ... site for miR -22 1 ⁄ 22 2 We found that overexpression of either miR -22 1 or miR -22 2 had no The present study has demonstrated that, in PC 12 cells, miR -22 1 and 22 2 are transcriptionaly induced upon ... either miR -22 1 or miR -22 2 resulted in significant (P < 0.05) FEBS Journal 27 6 (20 09) 326 9– 327 6 ª 20 09 The Authors Journal compilation ª 20 09 FEBS K Terasawa et al NGF induces miR -22 1 and 22 2 expression...

Ngày tải lên: 16/03/2014, 01:20

... Chem 27 4, 26 563 26 571 32 Terasawa K, Ichimura A, Sato F, Shimizu K & Tsujimoto G (20 09) Sustained activation of ERK1 ⁄ by NGF induces microRNA -22 1 and 22 2 in PC 12 cells FEBS J 27 6, 326 9– 327 6 33 ... Chem 27 5, 122 00– 122 06 FEBS Journal 27 7 (20 10) 22 29 ª 20 09 The Authors Journal compilation ª 20 09 FEBS ERK1 ⁄ in neuronal death S Subramaniam and K Unsicker 13 Lesuisse C & Martin LJ (20 02) Immature ... signal-regulated kinase to promote neuronal survival via the phosphatidylinositol 3 -kinase ⁄ protein kinase A ⁄ c-Raf pathway J Neurosci 25 , 28 38 28 52 26 Subramaniam S, Strelau J & Unsicker K (20 08) GDNF...

Ngày tải lên: 29/03/2014, 08:20

... protein kinase gene in breast cancer is FEBS Journal 27 7 (20 10) 74–80 ª 20 09 The Authors Journal compilation ª 20 09 FEBS A M Michie et al 23 24 25 26 27 28 29 30 31 32 33 34 associated with the invasive ... T, Schindler CK, Shinoda S, Lan JQ & Simon RP (20 03) Expression of death-associated protein kinase and recruitment to the tumor necrosis 78 12 14 15 16 17 18 19 20 21 22 factor signaling pathway ... activating DAPK activity [40] Indeed, bosutinib, an investigational Src kinase inhibitor, has been shown to inhibit the migration and invasion of breast cancer cells [48] In addition, dasatinib,...

Ngày tải lên: 29/03/2014, 08:20

... N.G (20 02) Distinct cell cycle timing requirements for extracellular signal-regulated kinase and phosphoinositide 3 -kinase signaling pathways in somatic cell mitosis Mol Cell Biol 22 , 722 6– 724 1 ... L’Allemain, G., Brunet, A ´ & Pouyssegur, J (1993) Growth Factors Induce Nuclear Translocation of MAP Kinases (p42maPk and p44mapk) but not of Their Activator MAP Kinase Kinase (p45mapkk) in Fibroblasts ... ETS-domain transcription factor contains a mitogen-activated protein kinase targeting motif Mol Cell Biol 18, 710– 720 Fukuda, M., Gotoh, Y & Nishida, E (1997) Interaction of MAP kinase with MAP kinase...

Ngày tải lên: 31/03/2014, 07:20

... in synergy with hantaviruses to inhibit ERK1/ 2 activity The additional effects of TNF-α on ERK1/ 2 inhibition were from 2- to 20 fold (Figure 4) Interestingly, TNF-α could inhibit ERK1/ 2 also in ... Journal 20 08, 5:110 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 Pleschka S, Wolff T, Ehrhardt C, Hobom G, Planz O, Rapp UR, Ludwig S: Influenza virus propagation is impaired by inhibition ... cleavage, TULV infection resulted in a MOI-dependent reduction in phosphorylated ERK1/ 2 (pERK1 /2) protein levels The magnitude of ERK1/ 2 inhibition correlated directly with increasing MOI and apoptosis...

Ngày tải lên: 20/06/2014, 01:20

... A specific inhibitor of phosphatidylinositol 3 -kinase, 2- (4-morpholinyl)-8-phenyl4H-1-benzopyran-4-one (LY2940 02) J Biol Chem 1994, 26 9: 524 1-8 Stein RC, Waterfield MD: PI3 -kinase inhibition: ... 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 Orisaka M, Orisaka S, Jiang JY, Craig J, Wang Y, Kotsuji F, Tsang BK: Growth differentiation factor is antiapoptotic during follicular ... pathway for luteinizing hormone and its interaction with insulin: activation of Janus kinase/ signal transducer and activator of transcription and phosphoinositol 3 -kinase/ Akt pathways Endocrinology...

Ngày tải lên: 20/06/2014, 07:20