erk and sodium proton exchanger type 1 nhe1
Redox regulation of estrogen receptor alpha and sodium hydrogen exchanger 1 gene expression by hydrogen peroxide
Ngày tải lên :
10/09/2015, 15:49
... REGULATION OF NHE1 ………… 11 0 3B .1 DOWN-REGULATION OF NHE1 BY H2O2 11 0 3B .1. 1 Identification of crucial region in the promoter of NHE1 11 0 3B .1. 2 H2O2 down-regulates 0 .15 kb proximal NHE1 promoter ... 0 .15 kb NHE1 promoter activity and NHE1 mRNA level 12 0 Figure 39: Reducing agent BME prevents H2O2-induced down-regulation of 0 .15 kb NHE1 promoter activity and NHE1 mRNA level 12 1 ... activity 12 4 3B .1. 5 Role of caspases in H2O2-mediated down-regulation of 0 .15 kb NHE1 proximal promoter activity 12 5 3B .1. 6 15 d-PGJ2 down-regulates 0 .15 kb NHE1 promoter activity and NHE1 mRNA...
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báo cáo hóa học:" The cross-sectional associations between sense of coherence and diabetic microvascular complications, glycaemic control, and patients’ conceptions of type 1 diabetes" pot
Ngày tải lên :
20/06/2014, 15:20
... SES, = yes 1. 50 (0.76 - 2.97) 1. 96 (0.95 - 4.05) 1. 14 (0.59 - 2. 21) 1. 39 (0.70 - 2.75) 0. 81 (0.54 - 1. 23) HbA1c 1. 37 (1. 12 - 1. 69) 1. 32 (1. 08 - 1. 61) 1. 36 (1. 11 - 1. 67) 1. 17 (0.97 - 1. 40) NA Data ... - 1. 01) Weak SOC 2 .11 (1. 13 - 3.95) 0.80 (0.44 - 1. 45) 1. 15 (0.63 - 2 .11 ) 1. 23 (0.75 - 2.03) 1. 52 (1. 07 - 2 .17 ) Gender, = male Diabetes duration, years NA 1. 10 (1. 07 - 1. 13) NA 1. 04 (1. 01 - 1. 06) ... 1. 06) NA 1. 12 (1. 09 - 1. 15) NA 1. 11 (1. 08 - 1. 13) 0.86 (0.64 - 1. 15) 0.99 (0.98 - 1. 01) 1. 00 (0.98 - 1. 01) Model Age at onset, years 1. 00 (0.94 - 1. 03) 0.95 (0.92 - 0.99) 1. 00 (0.97 - 1. 03) 0.95...
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Báo cáo y học: "Nef-mediated enhancement of cellular activation and human immunodeficiency virus type 1 replication in primary T cells" docx
Ngày tải lên :
13/08/2014, 01:21
... 240 0 18 10 00 800 42 18 0 12 0 60 12 15 18 21 200 5C 5C-3 5C-AxxA 400 14 600 28 p24 (ng/ml) SF SF2 Nef 70 12 15 18 21 6I -1 6I 56 300 240 12 15 18 400 6I -1 6I 18 0 12 0 60 80 0 12 15 18 21 160 14 240 ... 85, 89, Page of 17 90, 18 6 ,18 7 ,18 8, and 19 1 [48] Mutation of residue 19 1, however, disrupts Nef association with Vav [ 31] and SFKs [ 51] Mutations at position 19 1 (F191H and F191R) not abrogate ... http://www.retrovirology.com/content/8 /1/ 64 A Page of 17 Pre-Stim PHA-P (1 μg/ml) μg/ml PHA-P (2 μg/ml) α-CD3/CD28 HLA-DR 70.4% 16 0 5,579 16 ,446 65 ,17 8 11 .7% CD25 11 .9% 35.7% 56.9% 57.9% 414 1, 132 12 9 98.9% 95 .1% 1, 144 CD3...
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THE MECHANISM OF PPARN3 MEDIATED DOWN REGULATION OF SODIUM HYDROGEN EXCHANGER 1 (NHE1) GENE EPXRESSION AND ITS INHIBITION BY ESTROGEN RECEPTOR n1 1
Ngày tải lên :
09/09/2015, 10:19
... 1. 1.4 Subtypes of PPARs 1. 1.5 Ligands and physiological functions of PPARα and PPARδ 1. 1.6 Ligands of PPARγ 1. 1.7 PPARγ and adipogenesis 10 1. 1.8 PPARγ and ... 12 1. 1.9 PPARs and cancer 13 1. 1 .10 PPARγ and breast cancer 15 1. 2 ESTROGEN RECEPTORS (ERS) 18 1. 2 .1 Identification and structures of ERs 18 1. 2.2 Mechanism ... proliferation and differentiation 34 1. 3.5 NHE1 and cell migration 35 1. 3.6 NHE1 and heart disease 35 1. 3.7 NHE1 and cancer 36 1. 3.8 Regulation of NHE1 activity...
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THE MECHANISM OF PPARN3 MEDIATED DOWN REGULATION OF SODIUM HYDROGEN EXCHANGER 1 (NHE1) GENE EPXRESSION AND ITS INHIBITION BY ESTROGEN RECEPTOR n1 2
Ngày tải lên :
09/09/2015, 10:19
... up-regulation in NHE1 mRNA and further treatment with 15 d-PGJ2 lead to 42% decrease in NHE1 mRNA from the control treated with NAC alone (Figure 11 C) In contrast, 15 d-PGJ2 caused 61% reduction in NHE1 mRNA ... ligand- 88 mediated down-regulation of NHE1 gene expression Besides, the presence of the putative PPRE on NHE1 promoter and PPARγ ligands’ ability to down-regulate NHE1 mRNA further implied NHE1 ... rescue in NHE1 mRNA by NAC (Figure 11 C), further confirming that ROS/RNS 10 5 contributes approximately 20% of the overall down-regulation of NHE1 transcription by 5M of 15 d-PGJ2 10 6 Figure 11 : ROS/RNS...
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THE MECHANISM OF PPARN3 MEDIATED DOWN REGULATION OF SODIUM HYDROGEN EXCHANGER 1 (NHE1) GENE EPXRESSION AND ITS INHIBITION BY ESTROGEN RECEPTOR n1 4
Ngày tải lên :
09/09/2015, 10:19
... Western blot NHE1 band intensity was normalized to β-actin (C) MDA-MB-2 31 (2X10 cells/6-well dishes) and MDA-MB-2 31 ERα+ (2X105 cells/6-well dishes) cells were treated with 15 d-PGJ2 for 16 h with ... expression of NHE1 was analyzed by Western blot NHE1 band intensity was normalized to β-actin (C) MCF-7 (3 X105 cells/6-well dishes) cells were exposed to different PPARγ ligands: 3µM 15 d-PGJ2, 10 µM ciglitazone ... protein and mRNA is mediated by estrogen recptor alpha 13 1 Figure 19 : Re-expression of ERα blocks PPARγ-mediated down-regulation of NHE1 expression in MDA-MB-2 31 cells (A) MDA-MB-2 31 (2X105 cells/6-well...
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THE MECHANISM OF PPARN3 MEDIATED DOWN REGULATION OF SODIUM HYDROGEN EXCHANGER 1 (NHE1) GENE EPXRESSION AND ITS INHIBITION BY ESTROGEN RECEPTOR n1 6
Ngày tải lên :
09/09/2015, 10:19
... Diabetologia 51( 11) : 210 8 211 6 Jurka, J and A Milosavljevic (19 91) "Reconstruction and analysis of human Alu genes." J Mol Evol 32(2): 10 5 -12 1 Kallinowski, F., P Vaupel, et al (19 88) "Glucose ... (Neri and Supuran, 2 011 ; Webb et al., 2 011 ) For this, two strategies have been proposed: direct pharmacological inhibitors of NHE1 activity or controlling the gene/protein expression of NHE1 18 6 ... 14 64 -14 76 Badawi, A F and M Z Badr (2003) "Expression of cyclooxygenase-2 and peroxisome proliferator-activated receptor-gamma and levels of prostaglandin E2 and 15 deoxy-delta12 ,14 -prostaglandin...
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THE MECHANISM OF PPARN3 MEDIATED DOWN REGULATION OF SODIUM HYDROGEN EXCHANGER 1 (NHE1) GENE EPXRESSION AND ITS INHIBITION BY ESTROGEN RECEPTOR n1 3
Ngày tải lên :
09/09/2015, 10:19
... MDA-MB-2 31 (2 X 10 5 cells/6-well dishes) and T47D (3 X105 cells/6-well dishes) cells were treated with 15 d-PGJ2, for 16 h before they were washed and reseeded at a density of 1. 0 X 10 4 cells /10 0mm ... MCF-7 (3 X10 cells/6-well dishes) cells were exposed to (A) 15 d-PGJ2 and (B) ciglitazone for 16 h before they were washed and reseeded at a density of 1. 0 X104 cells /10 0mm dish After 14 days of ... of 15 d-PGJ2 treatment for 16 h before the colony forming assay was performed as described in Materials and Methods Similar to what we observed in MCF-7, 15 d-PGJ2 resulted in a dose-dependent 11 1...
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THE MECHANISM OF PPARN3 MEDIATED DOWN REGULATION OF SODIUM HYDROGEN EXCHANGER 1 (NHE1) GENE EPXRESSION AND ITS INHIBITION BY ESTROGEN RECEPTOR n1 5
Ngày tải lên :
09/09/2015, 10:19
... down-regulation of NHE1 expression in ER positive MCF-7 cells 13 8 13 9 Figure 21: Reduced ERα level enhances PPARγ-mediated down-regulation of NHE1 in regular serum conditon (A) MCF-7 (1. 5 X105 cells/6-well ... with 15 d-PGJ2 for 48h with or without 2h pre-incubation of 10 nM Fulvestrant in medium containing 10 % regular serum, and the protein expressions of NHE1 was analyzed by Western blot NHE1 band intensity ... down the NHE1 mRNA expression However, the same concentration of 15 d-PGJ2 resulted in 20% and 30% reduction in NHE1 mRNA level when cells were pre-incubated with 10 nM fulvestrant and 10 0nM raloxifene...
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Tài liệu Báo cáo khoa học: TMPRSS13, a type II transmembrane serine protease, is inhibited by hepatocyte growth factor activator inhibitor type 1 and activates pro-hepatocyte growth factor pdf
Ngày tải lên :
15/02/2014, 01:20
... factor activator FEBS Journal 277 (2 010 ) 4888–4900 ª 2 010 The Authors Journal compilation ª 2 010 FEBS T Hashimoto et al 10 11 12 13 14 15 16 17 18 inhibitor -1 (HAI -1) is essential for the integrity ... HAI -1 and HAI-2 against the protease activity of TMPRSS13 (A) Schematic representation of the structure of the full-length HAI -1 (1) and soluble forms of HAI -1, HAI -1 NK1LK2 (2) and HAI -1 NK1 (3), ... fluorometer (14 20 ARVOsx; Perkin Elmer Life Science, Boston, MA, USA) HAI -1 NK1, HAI -1 NK1LK2 and HAI-2 were prepared as follows The expression vectors encoding HAI -1 NK1, HAI -1 NK1LK2 and HAI-2...
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Prevalence of Celiac disease in Turkish children with type 1 Diabetes Mellitus and their non-diabetic first-degree relatives pptx
Ngày tải lên :
05/03/2014, 12:20
... Offspring ( 913 ) Control ( 71) 10 .1 3.5 (32) 1. 3 (11 ) Jaeger et al 20 01 Germany (7) IgA-tTG, IgA &IgG-AGA Diabetic children (19 7) Parent, sibling and offspring (882) Control (15 0) 16 .8 7.3 4.6 ... al 20 01 Italy (14 ) Not et al 20 01 Italy (8) AEA; Biopsy Williams et al 20 01 UK (10 ) IgA-AEA; IgA-tTG 34 14 5.7 (28) 1. 9 (16 ) 0.25 (10 ) Diabetic children (433) Parent (8 71) and sibling (5 71) Control ... (69) Control (10 3) 0.05 (25) (10 ) (5) (3) Diabetic children and adult (4 91) Parent, sibling and offspring (824) Control (4000) p 5.7 (28) 1. 9 (16 ) 0.25 (10 )
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Báo cáo khóa học: ICAM-1 expression is highly NF-jB-dependent in A549 cells No role for ERK and p38 MAPK docx
Ngày tải lên :
07/03/2014, 15:20
... stimulated with TNFa (10 ngÆmL )1) or IL-1b (1 ngÆmL )1) and harvested at 0, 2, and 18 h post stimulation, prior to Western blot analysis of ICAM -1 A representative blot is shown and optical densities ... 000 and : 10 00, respectively Alternatively, membranes were probed for phospho-hsp27 (#24 01) , or ERK 1/ 2 (# 910 0) Ó FEBS 2004 The role of NF-jB and MAPK in ICAM -1 expression (Eur J Biochem 2 71) ... inhibitors, PD098059 and U 012 6, were validated by analysis of phosphorylation of ERKs and 2, which lie downstream of MEK1 [24] Both TNFa and IL-1b upregulated phosphorylation of p42/44 ERKs and preincubation...
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Báo cáo Y học: Characteristics of binding of insulin-like growth factor (IGF)-I and IGF-II analogues to the type 1 IGF receptor determined by BIAcore analysis pptx
Ngày tải lên :
08/03/2014, 22:20
... Ala 31 des-(2,3)-Ala 31 Leu60 Ala31Leu60 IGF-II and analogues IGF-II Des- (1 6) Arg6 Leu27 Des- (1 6 ,10 )-Leu27 kd ´ 10 )3 (1/ s) Kd ´ 10 )9 (M) Relative Kd 2.70 2.52 3.60 3.65 2.89 ± ± ± ± ± ± 1. 16 1. 07 ... ± ± ± ± ± ± 1. 16 1. 07 0.88 1. 90 0.94 ± ± ± ± ± ± 4.29 4.25 2.44 5.20 3.25 88.6 16 1.0 53.4 48.2 945.0 ND 1. 00 1. 01 1.76 0.83 1. 32 0.05 0.03 0.08 0.09 0.005 ND 1. 00 0. 51 0.97 ± ± 2.35 2.90 2.25 ... 10 0 20 80 20 50 450 50 850 B 450 850 40 F 18 0 13 0 10 0 70 20 10 50 450 850 0 50 450 850 G C 18 0 50 10 0 30 10 20 50 450 850 D 50 450 850 H 18 0 50 10 0 30 10 20 50 450 850 Time (s) 50 450 Response...
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Báo cáo khoa học: Mutual effects of proton and sodium chloride on oxygenation of liganded human hemoglobin Oxygen affinities of the a and b subunits potx
Ngày tải lên :
16/03/2014, 14:20
... 31 ± 76 ± 18 ± 82 ± 1. 0 32 ± 84 ± 15 ± 85 ± 2.0 35 ± 10 6.4 ± 1. 3 10 .8 ± 1. 6 89.2 ± 1. 6 20 2.0 38 ± 10 0 ± 11 ± 89 ± 10 0 None 27.9 ± 1. 3 63.2 ± 0.9 22 ± 78 ± 0.5 16 .6 ± 1. 1 64.6 ± 1. 5 13 .9 ± 1. 2 ... 1. 2 86 .1 ± 1. 2 1. 0 11 .9 ± 1. 1 70 ± 11 .3 ± 0.4 88.7 ± 0.4 2.0 16 .1 ± 1. 1 94 ± 10 .0 ± 0.3 90.0 ± 0.3 None 29.0 ± 1. 5 65.0 ± 1. 8 26 ± 74 ± 0.5 8.5 ± 0.2 59 .1 ± 0.5 14 .5 ± 0.3 85.5 ± 0.3 1. 0 10 .2 ± ... ± 1. 4 11 .8 ± 0.2 88.2 ± 0.2 2.0 12 .9 ± 0.4 91. 2 ± 1. 0 11 .8 ± 0.3 88.2 ± 0.3 2.0 19 ± 90 ± 9±2 91 ± 7.4 6.8 10 0 20 M k¢a lM )1 s )1 k¢b lM )1 s )1 aa % ab % ca, daa 10 )2, 10 )2 cb, dba 10 )2, 10 )2...
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Báo cáo khoa học: Isolation, characterization, sequencing and crystal structure of charybdin, a type 1 ribosome-inactivating protein from Charybdis maritima agg. potx
Ngày tải lên :
16/03/2014, 14:20
... (A3) 49.6 1. 60 (1. 69 1. 60) 16 2385 (13 536) 4.6 (3.2) 6.4 (18 .6) 18 .1( 5.9) 20 1. 60 (1. 64 1. 60) 33 815 (19 71) 97.2 (77.6) 18 .1 (17 .8) 20.8 (20.8) 2 312 2047 253 12 18 .25 18 .86 0. 012 1. 607 0 .16 4 The ... the N-terminus and three turns comprising amino-acid residues 48–56, 96 10 2 and 18 3 18 8 Residues 1 3 and 99 10 1 are not included in the final refined model Fig Nucleotide sequence and derived amino-acid ... momordin (1MOM); lec, mistletoe lectin (1TFM); tri, trichosanthin (1MRJ); ric, ricin (1J1M); bry, bryodin (1BRY); pa3, pokeweed pap-III (1LLN); agg, agglutinin (1RZO); luf, luffin (1NIO); dia,...
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Báo cáo Y học: The reactivity of a-hydroxyhaem and verdohaem bound to haem oxygenase-1 to dioxygen and sodium dithionite pdf
Ngày tải lên :
23/03/2014, 21:20
... and Yang Am J Pathol 15 6, 14 85 14 88 Cary, S.P.L & Marletta, M.A (20 01) The case of CO signaling: why the jury is still out J Clin Invest 10 7, 10 71 10 73 Yoshida, T., Noguchi, M & Kikuchi, G (19 80) ... physiological degradation of haem Hence, the use of sodium dithionite should be avoided in the study of the haem oxygenase reaction 10 11 12 13 14 15 16 ACKNOWLEDGEMENTS This work was supported in ... phosphate buffer (pH 7.4) and allowed to stand for 30 min, prior to addition of 0.25 mL of the rHO -1 solution (10 3 nmol) The final concentrations of rHO -1 and a-hydroxyhaemin were 10 3 and 38 lM, respectively...
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Báo cáo khoa học: Cloning of type 1 cannabinoid receptor in Rana esculenta reveals differences between genomic sequence and cDNA pot
Ngày tải lên :
30/03/2014, 08:20
... 81. 9 74.2 73 73.4 73.2 72.2 72.6 72.5 66.6 70.8 12 72 14 28 14 07 14 13 14 22 14 13 14 22 14 19 14 22 14 22 14 19 14 19 14 18 12 36 13 20 21. 5 70.7 73.9 61. 9 82.9 88 .1 84.4 82.2 83.3 83.3 82.4 82.4 82.4 74.4 80.2 ... cnr1b, CAAB 010 014 84 .1; Danio rerio, NW_634056 .1; Mus musculus, NT _10 9 315 .2; Rattus norvegicus, NW_047 711 .2; Felis catus, NM_0 010 093 31. 1; Pan troglodytes, NW _10 7960 .1; Homo sapiens, NT_086697 .1 ... CNR1 receptor gene (cnr1) have been cloned and sequenced in mammals [10 ] Furthermore, cnr1 orthologs have been cloned and sequenced in fish [11 13 ], in urodele and anuran amphibians [14 ,15 ], and...
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Báo cáo sinh học: " Attenuation and efficacy of human parainfluenza virus type 1 (HPIV1) vaccine candidates containing stabilized mutations in the P/C and L genes" potx
Ngày tải lên :
18/06/2014, 18:20
... 0.5 14 .8 ± 1. 2 11 .0 ± 2.5 6.0 ± 1. 3 10 HPIV1 wt rHPIV1-CR84G rHPIV1-CR84GHNT553A rHPIV1-C 17 0 rHPIV1-LY942A rHPIV1-CR84GHNT553ALY942A rHPIV1-CR84GL 17 10 11 rHPIV1-CR84G/ 17 0HNT553ALY942A rHPIV1-CR84G/ ... 6h HPIV1 wt rHPIV1-CR84G rHPIV1-CR84GHNT553A rHPIV1-C 17 0 rHPIV1-LY942A rHPIV1-CR84GHNT553ALY942A rHPIV1-CR84GL 17 10 11 rHPIV1-CR84G/ 17 0HNT553ALY942A rHPIV1-CR84G/ 17 0HNT553AL 17 10 11 Mean sum ... rHPIV1-LY942A and rHPIV1CR84GL 17 10 1, Groups and in Table 2) This indicates that combining the non-ts and ts mutations in rHPIV 1and rHPIV1-CR84G/ CR84G/ 17 0HNT553ALY942A 17 0HNT553AL 17 10 11 did...
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Báo cáo sinh học: " Oligomerization of the human immunodeficiency virus type 1 (HIV-1) Vpu protein – a genetic, biochemical and biophysical analysis" ppt
Ngày tải lên :
18/06/2014, 18:20
... cytoplasmic domain J Virol 19 96, 70:2669-2673 Page 10 of 11 (page number not for citation purposes) Virology Journal 2007, 4: 81 10 11 12 13 14 15 16 17 18 19 20 21 22 Cohen EA, Terwilliger EF, Sodroski ... HIV -1, vpu, and its 16 -kilodalton product Science 19 88, 2 41: 12 21- 1223 Bour S, Strebel K: The human immunodeficiency virus (HIV) type envelope protein is a functional complement to HIV type 1Vpu ... Med 20 01, 19 4 :12 99 -13 11 Klimkait T, Strebel K, Hoggan MD, Martin MA, Orenstein JM: The human immunodeficiency virus type 1- specific protein vpu is required for efficient virus maturation and release...
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Báo cáo sinh học: "Detailed genetic analysis of hemagglutinin-neuraminidase glycoprotein gene in human parainfluenza virus type 1 isolates from patients with acute respiratory infection between 2002 and 2009 in Yamagata prefecture, Japa" docx
Ngày tải lên :
18/06/2014, 18:20
... Y04 /19 07 77 Y04 /10 71 Y04 /19 07 Y04 /10 71 Y04 /13 79 Y05 /15 04 81 Y05 /10 29, Y05 /10 59, Y05 /10 94 Y05 /10 33, and others (23) Y05/ 210 7 Y06/379 Y06 /16 1, Y06/4 51 Y06/55 Y05 /18 49, Y05/2702 Y05/2637 96 Y07 /14 37, ... Y07/2354 94 Y08/ 413 Y07/2072 Y07/2274 Y09/2225, and others (4) Y09/2 019 Y09 /12 92, and others (10 ) 74 Y09 /16 53 Y09 /12 51 Y09 /15 42 Y09 /14 42 Y09 /16 60, Y09 /16 86 Y09 /17 86 74 Y09 /14 41 Y09 /14 12, and others ... Y03/ 210 1 Y04 /17 73 Y04/759 Y03 /13 23, Y03/2066, Y03 /16 65 Y03 /14 46 Y03/2030 Y05 /11 09, and others ( 21) Y05 /17 89 84 Y05 /13 46 97 Y02 /14 33,Y02 /15 65 Y03 /11 22 70 Mil-48/ 91 (U70936) Washington /19 64 (AF45 710 2)...
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