epithelial mesenchymal transition in colorectal cancer

Epithelial mesenchymal transition in breast cancer progression

Ngày tải lên : 05/10/2015, 21:24

... keratin 14 overexpression in IDC 110 Figure 29 Maspin immunostaining in normal breast epithelia 118 Figure 30 Maspin immunostaining in DCIS of the breast 119 Figure 31 Maspin immunostaining in ... breast 99 Figure 25 Keratin 14 immunostaining in normal breast and skin 107 Figure 26 Keratin 14 immunostaining in DCIS of the breast 108 Figure 27 Keratin 14 immunostaining in IDC of the breast ... QRT-PCR 81 Figure 18 N-cadherin immunostaining in normal breast epithelia 85 Figure 19 N-cadherin immunostaining in DCIS of the breast 86 Figure 20 N-cadherin immunostaining in IDC of the breast 87...

161
480
0

Báo cáo y học: " Effects of PPARg ligands on TGF-b1-induced epithelial-mesenchymal transition in alveolar epithelial cells" ppsx

Ngày tải lên : 12/08/2014, 11:20

... TGF-b1-mediated increases in aSMA expression and fibrillar collagen synthesis [8,9] Similar findings have been observed in skin fibroblasts [19], with PPARg ligands inhibiting TGF-b1 signalling via the ... alveolar epithelial cell line A549 (ATCC, Manassas, VA) was maintained in high glucose-DMEM (Invitrogen, Carlsbad, CA) containing 10% (v/v) FBS, 15 mM HEPES, 20 mM sodium bicarbonate, and mM Lglutamine ... poly-L-lysine solution After overnight attachment, cells were maintained in serum-free DMEM containing 0.25% (w/v) bovine serum albumin (BSA) for 24 hr PPARg ligands were prepared as 30 mM stocks in...

13
242
0

Báo cáo y học: " Detection of epithelial to mesenchymal transition in airways of a bleomycin induced pulmonary fibrosis model derived from an α-smooth muscle actin-Cre transgenic mouse" potx

Ngày tải lên : 12/08/2014, 16:20

... arrowhead), βgal staining was not detected in terminal bronchioles, although small veins were positively stained (c) The thin layer of βgal staining was observed in the sub -epithelial areas of ... gene in these cells Additionally, βgal was stained positively in a few basal epithelial cells and columnar epithelial cells lining the bronchiole during bleomycin-induced lung fibrosis in the ... elastin The staining was carried out using Masson trichrome staining Kit (Maxim-Bio, Fuzhou, China) according to the manufacturer's instruction Western Blot α-SMA protein levels in lungs were...

11
433
0

Báo cáo khoa học: The in vitro effects of CRE-decoy oligonucleotides in combination with conventional chemotherapy in colorectal cancer cell lines potx

Ngày tải lên : 07/03/2014, 15:20

... bovine serum GEO colorectal cancer cell lines were a gift from G Tortora (Dipartimento di Endocrinologia e Oncologia Molecolare e Clinica, ` Universita di Napoli, Italy), and were maintained in ... synergistic anticancer effects in colorectal cancer cell lines We conﬁrmed that CDO was cytotoxic in two of the cell lines studied at nanomolar concentrations Additionally, we showed that combining CDO ... that CDO alone did not increase the extent of staining However, in cells that had been cocultured with CDO and a cytotoxic drug, staining was signiﬁcantly increased, indicating the presence of senescence...

9
331
0

Guidance on Cancer Services – Improving Outcomes in Colorectal Cancers – Manual pdf

Ngày tải lên : 28/03/2014, 23:20

... NHS National Institute for Clinical Excellence Guidance on Cancer Services Improving Outcomes in Colorectal Cancers Manual Update Improving Outcomes in Colorectal Cancers Cancer service guidance ... three main tasks The first was to breathe new life into dialogue about services for managing bowel cancer by highlighting key issues and enhancing and clarifying the original text In refocusing attention ... cancer deaths in the 34 35 36 37 38 20 Bingham SA, Day NE, Luben R, Ferrari P, et al Dietary fibre in food and protection against colorectal cancer in the European Prospective Investigation into...

136
302
0

Báo cáo hóa học: " LEF-1 and TCF4 expression correlate inversely with survival in colorectal cancer" pptx

Ngày tải lên : 18/06/2014, 16:20

... is found in human colorectal cancer [14], a shift of b-catenin binding partners from TCF4 to LEF-1 might occur during carcinogenesis which might enable enhanced epithelial- mesenchymal transition ... These surprising findings suggest that TCF4 might be the main binding partner for b-catenin during development and progression of colorectal cancer whereas an enhancement of Wnt/bcatenin transcriptional ... TCF4 expression in colorectal cancer To investigate the localisation of LEF-1 and TCF4 in human colorectal cancer, we evaluated the expression of these proteins by immunostaining on tissue microarrays...

8
664
0

Báo cáo sinh học: "A predicted protein, KIAA0247, is a cell cycle modulator in colorectal cancer cells under 5-FU treatment" ppt

Ngày tải lên : 18/06/2014, 19:20

... effect induced by 5-FU in the KIAA0247expressing cells could be crucial in controlling the G2/ M checkpoint and in decreasing the number of cells in G2/M At the same time, reduced levels of cyclins ... Chloroquine potentiates the anticancer effect of 5-fluorouracil on colon cancer cells BMC Cancer 2010, 10:370 35 Cunningham D, Atkin W, Lenz HJ, Lynch HT, Minsky B, Nordlinger B, Starling N: Colorectal ... Tabernero J: Novel targets for anticancer treatment development in colorectal cancer Clin Colorectal Cancer 2006, 6:265-272 Voutsadakis IA: Pathogenesis of colorectal carcinoma and therapeutic implications:...

8
357
0

Báo cáo sinh học: "Protein kinase CK2a is overexpressed in colorectal cancer and modulates cell proliferation and invasion via regulating EMT-related genes" docx

Ngày tải lên : 18/06/2014, 19:20

... in colorectal cancer CK2a protein expression was analyzed in 144 patients (104 with CRC and 40 with colorectal adenoma) Staining for CK2a was nearly negative in all of the normal Page of 11 colorectal ... expression in colorectal cancers, adenomas and adjacent normal colorectal mucosa Staining was (A) negative in normal colorectal epithelium cells, (B, C) weak to moderate in the nuclei of colorectal ... Murray T, Thun MJ: Cancer statistics, 2008 CA Cancer J Clin 2008, 58:71-96 Sung JJ, Lau JY, Goh KL, Leung WK: Increasing incidence of colorectal cancer in Asia: implications for screening Lancet Oncol...

11
382
0

báo cáo hóa học:" A predicted protein, KIAA0247, is a cell cycle modulator in colorectal cancer cells under 5-FU treatment" doc

Ngày tải lên : 20/06/2014, 03:20

... effect induced by 5-FU in the KIAA0247expressing cells could be crucial in controlling the G2/ M checkpoint and in decreasing the number of cells in G2/M At the same time, reduced levels of cyclins ... Chloroquine potentiates the anticancer effect of 5-fluorouracil on colon cancer cells BMC Cancer 2010, 10:370 35 Cunningham D, Atkin W, Lenz HJ, Lynch HT, Minsky B, Nordlinger B, Starling N: Colorectal ... Tabernero J: Novel targets for anticancer treatment development in colorectal cancer Clin Colorectal Cancer 2006, 6:265-272 Voutsadakis IA: Pathogenesis of colorectal carcinoma and therapeutic implications:...

8
356
0

Báo cáo khoa học: "Does Doxycycline work in synergy with cisplatin and oxaliplatin in colorectal cancer?" ppt

Ngày tải lên : 09/08/2014, 04:20

... apoptosis in colorectal cancer cell lines [13,14] In this study we investigated whether doxycycline works in synergy with cisplatin and oxaliplatin or not in HT 29 colorectal cancer cell line Methods ... activity levels in cells treated with cisplatin and oxaliplatin in combination with doxycycline (p = 0.001 and p = 0.039 respectively) These findings corresponded with the findings obtained in caspase ... Discussion Colorectal cancer constitutes the third most common cancer and the second leading cause of cancer deaths in UK About one in 20 men develops colorectal cancer in their lifetime (Cancer...

8
252
0

Báo cáo y học: "Relevance of the stroma and epithelial-mesenchymal transition (EMT) for the rheumatic diseases" docx

Ngày tải lên : 09/08/2014, 08:22

... matrix protein laminin-5 induced EMT and hepatocellular carcinoma cell invasion by upregulating Snail and Slug, down regulating E-cadherin, translocating β-catenin into nuclei, and inducing dramatic ... Wnt/β-catenin signaling pathway In resting cells, glycogen synthase kinase (GSK3β) is in a complex with CK1, β-catenin, axin and adenomatous polyposis coli protein In this state, β-catenin is primed ... Conversely, mesenchymal cell lines become epithelial when transformed by E-cadherin genes [6] The wnt signaling pathway regulates the amounts of β-catenin protein available within the cell for binding...

11
622
0

báo cáo khoa học: "T cell subpopulations in lymph nodes may not be predictive of patient outcome in colorectal cancer" pps

Ngày tải lên : 10/08/2014, 10:21

... suppression in colorectal cancer patients Clin Cancer Res 2010, 16:4105-4112 Ohtani H: Focus on TILs: prognostic significance of tumor infiltrating lymphocytes in human colorectal cancer Cancer Immun ... Foxp3+ cells This indicates that the Treg population was not diminished in IBD patients, a finding in direct contrast to Clarke et al We are currently investigating this further to examine the role ... effector T cell responses in tumour draining lymph nodes [27] Recent data also indicated that the presence of Foxp3+ T cells in tumour draining lymph nodes of colorectal cancer patients correlated...

7
257
0

báo cáo khoa học: "Individualized therapies in colorectal cancer: KRAS as a marker for response to EGFR-targeted therapy" docx

Ngày tải lên : 10/08/2014, 22:20

... transmembrane glycoproteins: the extracellular domain has the ligand binding site and also contains specific sequences that are involved in dimerization, while the intracellular domain is a catalytic ... detected in many human cancers, including CRC Monoclonal antibodies that target EGFR can be effective as anticancer therapy in several ways They can block ligand binding to the receptor, aborting ... predictors for response to cetuximab containing chemotherapy in colorectal cancer patients Identification of new KRAS mutation in codon12 ASCO 2008 Gastrointestinal Cancers Symposium 2008 Tejpar S, Peeters...

9
311
0

báo cáo khoa học: " Transcriptome instability in colorectal cancer identified by exon microarray analyses: Associations with splicing factor expression levels and patient survival" docx

Ngày tải lên : 11/08/2014, 12:21

... of deviating skipping -3 -2 -1 Relative amounts of deviating inclusion skewed TIN (sTIN): Preferential exon skipping or inclusion overall TIN (oTIN): High or low total amounts of deviating exon ... deviating skipping 1.5 0.5 -2.5 -2 -1.5 -1 -0.5 0.5 Relative amounts of 1.5 deviating inclusion -0.5 -1 -1.5 -2 skewed TIN (sTIN): Preferential exon skipping or inclusion overall TIN (oTIN): High ... (Table S5A in Additional file 2) However, patients with either sTIN or oTIN cancers (a) Splicing Stage MSI-status sTIN oTIN (b) Stage II CRC / MSS tumors / no sTIN / no oTIN Splicing Stage MSI-status...

13
283
0

Role of stathmin 1 in colorectal cancer metastasis and chemo resistance

Ngày tải lên : 09/09/2015, 11:25

... 10% of all cancer incidence and mortality in 20132 Consistent with trends in developed countries, CRC is the most frequent cancer in Singapore, accounting for 14% to 18% of all cancer incidence ... 2005-20093 Colorectal cancers usually begin as non-cancerous polyps in the inner lining of the colon or rectum4 Over many years, adenomatous polyps in the dysplasic colon may develop into pre-neoplastic ... xiii Chapter Introduction 1.1 Colon cancer 1.1.1 Colorectal carcinoma Colorectal carcinoma (CRC) is a perennial concern in public health1 According to yearly estimates from the American Cancer Society...

175
634
0

Identification of a new tumor suppressor pathway modulating rapamycin sensitivity in colorectal cancer

Ngày tải lên : 09/09/2015, 18:52

... and the role of this pathway in rapamycin sensitivity in cancer therapy; To examine the combination therapy by using PDK1 inhibitor and mTOR inhibitor in colorectal cancer The present study identifies ... signaling and oncogenic transformation, making PIK3CA a druggable target in cancer therapy (Liu et al., 2009) Many PI3K inhibitors have been developed in clinical setting, including pan-PI3K inhibitors, ... strategy in treating rapamycin-resistant colorectal cancer 32 Chapter II: Materials and Methods 33 2.1 Cell lines and cell culture 2.1.1 Colorectal cancer cell lines The human colorectal cancer...

200
331
0

Runx3 protects gastric epithelial cells against epithelial mesenchymal transition induced cellular plasticity and tumorigenicity

Ngày tải lên : 09/09/2015, 18:57

... event in early gastrointestinal carcinogenesis 10 1.3 The involvement of RUNXs in major signaling pathways 1.3.1 RUNXs are integral components of the TGF-β/SMAD signaling cascade Transforming growth ... reflected in the increased intestinal tumour incidence and mass in Runx3+/-.Apcmin/+ compound mutant mice (Ito et al., 2008) Consistent with this, increased Wnt signaling activity was observed in BALB/c ... fluorescent protein epithelial- mesenchymal transition epithelial cell adhesion molecule E-cadherin forward fluorescence activated cell sorting fetal bovine serum fibroblast growth factor fibronectin glycogen...

172
201
0

Role of CC3 in colorectal cancer progression

Ngày tải lên : 14/09/2015, 08:50

... GTBP G/T binding protein TIMP Tissue Inhibitor of Metalloproteinases DISC Death-Inducing Signaling Complex Apaf-1 Apoptotic protease-activating factor-1 IAP Inhibition of Apoptosis Proteins Smac ... factors in colorectal cancer, molecular markers may also be useful prognostic and predictive indicators in colorectal cancer as they have been shown to be in other cancers In breast cancer, for ... alteration In animals, methylcytosine is mainly found in cytosine-guanine (CpG) 19 dinuleotides(Bird 1986) The presence of 5-methylcytosine in the promoter of specific genes prevents binding of transcription...

169
188
0

Clinical reference of tumour infiltrating lymphocytes in colorectal cancer

Ngày tải lên : 03/10/2015, 20:57

... Genotyping SNP genotyping in clinical samples were carried out using the MassArray iPLEX genotyping platform (Sequenom, San Diego, CA) according to manufacturer‟s instructions SNPs, consisting of ... different amino acid or premature stop codon respectively For SNPs that are not in the proteincoding regions, they may still contribute to changes in gene splicing, transcription binding or affect ... was achieved by incubation with 3,30-diaminobenzidine (DAB) for 10 min, followed by incubation with Bond DAB enhancer For the double staining of GZMB/CD8 and CD4/FOXP3, heat-induced antigen retrieval...

88
137
0