epinephrine+norepinephrine+autoinducer+3+interkingdom+signaling+system+in+escherichia+coli+o157+h7

... 6, 87–1 03 33 Kuwajima K (1992) Protein folding in vitro Curr Opin Biotechnol 3, 462–467 34 Kuwajima K (1996) The molten globule state of a-lactalbumin FASEB J 10, 102–109 35 Yip CK, Finlay BB ... Escherichia coli O157 Biochem Soc Trans 31 , 98–1 03 Kenny B, DeVinney R, Stein M, Reinscheid DJ, Frey EA & Finlay BB (1997) Enteropathogenic E coli (EPEC) transfers its receptor for intimate adherence into ... from enterohemor- FEBS Journal 272 (2005) 27 73 27 83 ª 2005 FEBS 10 11 12 13 14 15 16 rhagic Escherichia coli O157: H7 Infect Immun 66, 38 10 38 17 Zhu C, Agin TS, Elliott SJ, Johnson LA, Thate TE,...

Ngày tải lên: 30/03/2014, 16:20

... Rifampicin (MIC) - E coli O157: H7 (3 × LD50) Gentamicin (MBC) - E coli O157: H7 (3 × LD50) Rifampicin (MIC) Gentamicin (MBC) E coli O157: H7 (3 × LD50) Rifampicin (MIC) Rifampicin (MBC) Trypticase ... O157: H7 CFU administered in vivo]/E coli O157: H7 CFU per in vitro MIC reaction Consequently, the ratio of rifampicin to E coli O157: H7 CFU determined by in vitro MIC testing was maintained in vivo ... 26-98 strain of E coli O157: H7 incubated in the presence of the MIC of rifampicin followed by the MBC of gentamicin E coli O157: H7 was grown for 18 hrs at 37 °C in rifampcin-containing TSB followed...

Ngày tải lên: 12/08/2014, 17:20

... 31 2-10 35 1-10 37 7-11 38 9-8 412-14 50- 13 175-21 204-16 2 53- 17 32 4-14 35 9-11 37 8-10 39 0-5 425-5 51- 13 176-12 229-8 254-4 32 5- 13 361- 13 379-12 39 1-8 426-5 52-17 177-11 231 -6 255-8 32 6-9 36 4-14 38 0- 13 ... 36 4-14 38 0- 13 392-11 427-7 54-15 178- 13 232 -7 256-8 32 7- 13 367-12 38 1-11 402-12 433 -11 55-16 179-21 242-12 257-9 32 8-12 36 8-8 38 2-10 4 03- 7 439 -11 69-9 189-10 2 43- 12 258-9 32 9-12 36 9-9 38 3-8 404-9 ... 232 -242, 262-268, 2 83- 291, 30 1 -30 9, 31 9 -32 9, 39 2-404, 448-475, 482-490, 512-526, 528-547, 430 -446 Flexibility 4-10, 25 -35 , 43- 51, 104-1 13, 199-214, 217-226, 279-287, 30 7 -31 4, 31 6 -32 5, 38 9-4 03, ...

Ngày tải lên: 13/08/2014, 16:20

... 480 460 440 420 400 38 0 36 0 34 0 32 0 30 0 280 260 240 220 200 180 160 140 120 100 80 60 40 20 bp Maker 500 400 30 0 200 114 y = -66613x + 294625x - 506 731 x + 431 852x - 186046x + 33 174x 100 80 R = 0.9998 ... pTYB12 MCoTI-II Transformed into E .coli BL21 (DE3) PI - 17 Fig 7: Scheme summarizing the steps in obtaining a construct system for expression of MCoTI-II To identify the insert gene, we isolated ... containing 100àg/ml ampicillin, incubated in shakers at 37 oC until the OD600 reached 0.6 Induction by IPTG Expression and purification of recombinant MCoTI-II were followed the manufactures instruction...

Ngày tải lên: 12/02/2014, 10:20

... metabolism of Escherichia coli Biotechnol Bioeng 79, 53 73 Mahadevan R, Edwards JS & Doyle FJ (2002) Dynamic flux balance analysis of diauxic growth in Escherichia coli Biophys J 83, 133 1– 134 0 Santillan ... lactose and glucose transport systems In this study, we compare two models describing catabolite repression in E coli by discussing some relevant issues of modelling in systems biology, model validation, ... lactose Metab Eng 3, 36 2 37 9 Covert IMW, Xiao N, Chen TJ & Karr JR (2008) Integrating metabolic, transcriptional regulatory and signal transduction models in Escherichia coli Bioinformatics 24,...

Ngày tải lên: 18/02/2014, 13:20

... and strains 23S rRNA single point mutations were initially constructed by PCR mutagenesis on plasmid pXB containing the E coli 23S rRNA gene with streptavidine binding tag [29] Mutant 23S rRNA ... pseudouridine 55 synthase Biochemistry 37 , 33 9 34 3 27 Lebars I, Yoshizawa S, Stenholm AR, Guittet E, Douthwaite S & Fourmy D (20 03) Structure of 23S rRNA hairpin 35 and its interaction with the tylosin-resistance ... base pair (U 33: A36) and base flipping of three bases including the substrate uridine 32 These structural elements are absent in normal tRNA RluA appears to recognize its substrate by indirect readout...

Ngày tải lên: 18/02/2014, 16:20

... 212.1 37 5.2 432 .2 595 .3 7 23. 3 810 .3 897.4 994.4 1065.5 1148.5 260.1 37 4.1 537 .2 594.2 757 .3 885 .3 972 .3 1059.4 1156.4 1227.5 131 0.5 422.1 536 .1 699.1 756.1 919.2 1047 .3 1 134 .3 1221 .3 131 8.4 138 9.4 ... PNYGYQSSPAX 98.1 212.1 37 5.2 432 .2 595 .3 7 23. 3 810 .3 897.4 994.4 1065.5 1148.5 260.1 37 4.1 537 .2 594.2 757 .3 885 .3 972 .3 1059.4 1156.4 1227.5 131 0.5 % 34 32 38 40 44 41 initiation of the prokaryotic ... ions b ions + 162 98.1 212.1 37 5.2 432 .2 595 .3 7 23. 3 810 .3 897.4 994.4 1065.5 1148.5 260.1 37 4.1 537 .2 594.2 757 .3 885 .3 972 .3 1059.4 1156.4 1227.5 131 0.5 % 36 39 39 42 Sequence b ions b ions...

Ngày tải lên: 19/02/2014, 16:20

... purification of E coli elongation factor Tu Biochem Int 28, 35 3 36 2 40 Sottrup-Jensen, L (19 93) Determination of halfcystine in proteins as cysteine from reducing hydrolyzates Biochem Mol Biol Int 30 , 789–794 ... known to be involved in either protein–protein interactions [61,62] or protein–RNA interactions [ 63] The proposed intramolecular interaction may be an integrated step in the guanine nucleotide-exchange ... pET11d that contains the EcoRI–PstI Intein chitin binding domain (CBD) fragment of plasmid pCYB3 from the IMPACT System (New England Biolabs) [37 ] In contrast to pCYB3, which contains one SapI restriction...

Ngày tải lên: 21/02/2014, 00:20

... Zn(II)binding motifs In addition to the known zinc-containing proteins, we obtained in this study experimental evidence showing Zn(II) binding for some E coli proteins, including acetate kinase ... Cobalamin-independent methionine synthase from Escherichia coli: a zinc metalloenzyme Biochemistry 35 , 12228– 12 234 Ó FEBS 2002 Escherichia coli zinc-binding proteins (Eur J Biochem 269) 24 13 41 ... Dose-dependent binding of Zn(II) to purified E coli zinc-binding proteins The newly identified E coli Zn(II)-binding proteins, AckA and Ppa, were analysed, together with the known Zn(II)-binding proteins,...

Ngày tải lên: 22/02/2014, 04:20

... 2.1 6 .3 0.15 1.6 6.8 28 3. 2 8.4 6.7 16 35 200 0.90 14 23 350 62 64 23 42 BL21(DE3) Rosetta(DE3) BL21(DE3) Rosetta(DE3) BL21(DE3) Rosetta(DE3) BL21(DE3) Rosetta(DE3) BL21(DE3) Rosetta(DE3) A O ... example, by using the E coli strain Rosetta(DE3) When comparing the protein production of five different proteins in E coli BL21(DE3) with the production in Rosetta(DE3), the Rosetta(DE3) strain generated ... 270 1000 280 290 38 0 470 530 33 0 430 39 0 640 39 0 660 79 0.46 66 39 140 34 0 72 55 95 160 120 120 77 88 210 110 160 0.29 0. 43 0.44 1.5 1.4 1.2 0.20 0.46 1.7 0.81 0. 23 0 .35 0.41 0 .30 1.5 0.48 1.8...

Ngày tải lên: 06/03/2014, 01:20

... R266C R318C R318A R318K R 437 C Salt bridge T382D/V385K Loop mutants G213V H222Q D223K a Sensing Vmax (nmolÆmg)1Æh)1) Membrane incorporation (% wild-type) 63 110 100 110 3. 8 135 30 23 46 82 33 145 ... 23 46 82 33 145 45 73 150 115 105 154 30 61 31 75 145 152 106 100 22 39 13 37 32 93 81 63 67 128 480 39 5 231 24 678 97 81 179 241 158 78 n.m 2646 0.86 3. 2 Constitutivea 7 .3 932 Constitutivea Constitutivea ... Determination of the K(induction) of the UhpT-inducing system E coli cells BL21(DE3) (A) and E coli cells BL21(DE3) (uhpC:: Tn1000) harbouring plasmid uhpC/pET16b (B) were induced for 15 with given...

Ngày tải lên: 08/03/2014, 08:20

... nascent chains were linearized and transcribed as described previously [ 13] The resulting mRNAs were translated in vitro in a lysate of strain MC4100 as described [ 13, 33] The mixture containing RNCs ... strains The E coli K-12 strains used in this study are listed in Table Strains CE1514 and CE1515 were obtained by P1 transduction using strain CE1224 as the recipient and strains IQ85 and strain ... in vitro, we subsequently examined whether these nascent chains are targeted to SecY via SRP by performing cross-linking experiments in vitro in the presence of IMVs To obtain a high cross-linking...

Ngày tải lên: 08/03/2014, 09:20

... glycine, glycine-ester and D-pipecolic acid was only observed using GO Interestingly, D-proline is the only amino acid in which the a-amino group is involved in a covalent bond with the side chain ... Y2 23 in DAAO and Y254 in MSOX Even the arginine residue (R285 in yeast DAAO) which electrostatically interacts with the a-carboxylate of the D-amino acid in DAAO [15,16] is conserved in GO (R302, ... N-termini (containing the Rossman fold fingerprint motif GXGXXG, which is involved in binding of the ADP moiety of FAD) [11] and for the 70 residues at their C-termini (for this latter region a 31 –40%...

Ngày tải lên: 08/03/2014, 16:20

... L Marcellini et al + Esc(1–18) and E coli membrane permeation/proteome A pH 3 10 – B B A C C 6707 67 03 2702 6707 6707 67 03 67 03 2702 56 03 56 03 56 03 2702 0407 0407 0407 51 03 51 03 51 03 Fig 12 Two-dimensional ... serum (8) 32 CFUÆmL)1 32 (> 128) 128 16 (32 ) 32 16 (4) 32 16 (8) 32 64 (> 128) 128 The killing kinetics occurred on a quite fast time scale, causing more than 90% microbial deaths within 10 min, at ... Carbenicillin Cephalosporin C Ceftazidime Chloramphenicol Erythromycin Imipenem Nalidixic acid Netilmicin Oxacillin Rifampicin Streptomycin Tazocin Tetracycline 64 128 0.5 32 0.5 8 8 4 1.06 0. 73 0.46...

Ngày tải lên: 16/03/2014, 00:20

... incubated in 100 mL of the latter buffer at 37 °C overnight Finally, nonhydrolysed azocasein in the polyacrylamide gel was stained with CBB Immunoblotting Bacterial lysates containing recombinant ... recombinant GluV8 in Escherichia coli would be useful in order to elucidate in detail the roles of the prepro- and C-terminal repeated segments, as well as specific amino acid residues, involved in ... as inhibition of the enzymatic activity Proc Natl Acad Sci USA 88, 933 0– 933 4 O’Donohue MJ & Beaumont A (1996) The roles of the prosequence of thermolysin in enzyme inhibition and folding in vitro...

Ngày tải lên: 16/03/2014, 06:20

... MglB 5.68 35 .7 Galactose-binding protein MalE 5.22 40.7 Maltose-binding protein Proteins induced at a lower level as compared with growth on acetate: AdhP 5.94 35 .4 Propanol-preferring alcohol ... Sequence coverage (%) P08997 P366 83 P27550 P77541 49 10 27 52 P31660 P772 43 P02927 P02928 22 49 38 71 P39451 P31217 P14178 54 39 50 SWISSPROT Function Proteins induced at a higher level as compared ... with previous 2DE data [33 ] (C) Alkaline sections of gels covering the pH range 3 10 and containing PrpC are displayed prepared from exponentially growing cells as described in Experimental procedures...

Ngày tải lên: 17/03/2014, 10:20

... DNA supercoiling Cell 34 , 105±1 13 Wang, J.C (1971) Interaction between DNA and an Escherichia coli protein omega J Mol Biol 55, 5 23 533 Sugino, A & Cozzarelli, N.R (1980) The intrinsic ATPase ... Roles of topoisomerases in maintaining steady state DNA supercoiling in Escherichia coli J Biol Chem 275, 81 03 81 13 Menzel, R & Gellert, M (19 83) Regulation of the genes for E coli DNA gyrase: homeostatic ... supercoiling was assessed in terms of the linking number of intracellular plasmid pBR322 [18] DNA supercoiling was expressed as the active linking number, aLk, which is the difference in linking number...

Ngày tải lên: 18/03/2014, 01:20

... 29 30 31 32 33 34 35 36 37 38 39 40 41 Alcaligenes faecalis, a molybdenum-containing hydroxylase J Biol Chem 267, 236 74– 236 82 Wysocki R, Chery CC, Wawrzycka D, Van Hulle M, Cornelis R, Thevelein ... may be caused by binding to zinc binding proteins, e.g proteins that contain zinc finger protein structures [30 ] Zinc and cadmium are in the same group in the Periodic Table, contain the same common ... did not result in their insertion into MPT (data not shown) In addition, we tested the insertion of metal ions into MPT-containing hSO None of the metals was inserted into hSO (Fig 3B), which is...

Ngày tải lên: 23/03/2014, 06:20

... cyclosporin A-binding protein cyclophilin Nature 33 7, 4 73 475 33 Schonbrunner ER, Mayer S, Tropschug M, Fischer G, Takahashi N & Schmid FX (1991) Catalysis of protein folding by cyclophilins from different ... 6, using ORIGIN software (MicroCal, Inc.) Protein Tm (°C) DHcal (kJÆmol)1) DHvH (kJÆmol)1) SIB1 FKBP22* 32 .5 46.4 35 .6 44.7 82.8 194.8 171.8 140.9 404.2 30 3.9 232 .4 259.2 C-domain+ N-domain+ exists ... the N-domain of SIB1 FKBP22 with entire a3 helix (N-domain+), C-domain without a3 helix (C-domain–), and C-domain with entire a3 helix (C-domain+), respectively, were constructed by ligating a part...

Ngày tải lên: 23/03/2014, 13:20

... 67 .3( 6), 45.1(8), 6.9 129 .33 5.91(d) 67.0(5), 46.6 (3) , 6.9 1 23. 10 5.99 nd 85.54 5. 23 44.9(7), 36 .5 (3) , 4.2(6) cis-D3-Dehydro[U-13C6]adipate (3) 1/6 nd 2/5 34 .76 2.95 51.6(1/6), 42.5 (3/ 4) 3/ 4 ... 3/ 4 125 .31 5.60 3- Hydroxy[U-13C6]adipate (4) or its lactone 176.82 50.2(2) 42 .36 2.52 52.0(1), 39 .9 (3) , 1.2 (6,4) 79.81 4.86 39 .8(2), 32 .1(4), 4.2(6) 27.01 2 .34 , 1.88 32 .1 (3. 5), 3. 2(1) 28. 53 2.59, ... mixtures containing 0.1 M phosphate, pH 6.5, 40 mg L-[U-13C9]phenylalanine or L-[ring-13C6]phenylalanine, 740 kBq [U-14C9]phenylalanine, and U L-amino acid oxidase (Fluka, Neu-Ulm, Germany) in a total...

Ngày tải lên: 23/03/2014, 18:20