enzymes involved in hydrolysis of lignocellulosic material

... (S)-cheilanthifoline, not (S)-nandinine [7,30,31] If we assume that (S)-nandinine is produced in E californica, there may be no need for a catalyst from (S)-nandinine to (S)-stylopine However, nandinine could ... bridge-forming P450 (CYP719) cDNA, the protein of which catalyzed the conversion of (S)-tetrahydrocolumbamine to (S)-tetrahydroberberine [(S)-canadine] in berberine biosynthesis, from cultured cells of ... and the flow rate (0.5 mLÆmin)1) To determine the kinetic parameters of CYP719A2, the amount of nandinine produced was estimated by using a calibration curve of (S)-scoulerine (pmol versus peak area)...

Ngày tải lên: 07/03/2014, 10:20

... displaying two putative splice variants [Fig 2] Expression of fucokinase cDNAs The two splice variants of fucokinase were subcloned into the XbaI site of a pQM vector containing a C-terminal E2-Tag/A ... variants of L-fucokinase The long splice variant of L-fucokinase consisted of 3270 bp, encoding a protein of 1090 amino acids The sequence of the shorter cDNA was similar to the sequence of the IMAGE ... alternative splicing maintains the reading frame along the entire protein, therefore the protein variants are identical in the amino-terminal end up to the alternative splice area, in addition to...

Ngày tải lên: 30/03/2014, 13:20

... isoalloxazine ring (Fig 5B, broken line, number 3) Phe in place of Trp66 resulted in a protein with noncovalently bound FAD, again showing no enzyme activity, and isolation of the flavin cofactor ... protein with covalently attached flavin, synthesized only in bacteria grown in the presence of nicotine The DNA carrying the sarcosine dehydrogenase-like ORF63, corresponding to a protein of 813 ... in the activity staining of native MABO on nondenaturing polyacrylamide gels soaked in 10 mL of 20 mM potassium phosphate buffer, pH 10, containing 10 mM c-N-methylaminobutyrate, 10 IUÆmL)1 of...

Ngày tải lên: 19/02/2014, 16:20

... A., Inoue, K., Hiraoka, N., Ikeshiro, Y., Kushiro, T., Shibuya, M & Ebizuka, Y (2001) Cloning and characterization of a cDNA encoding b-amyrin synthase involved in glycyrrhizin and soyasaponin ... Differential expression of OSC mRNAs in the cultured cells of L cylindrica Figure shows the multiple alignments of deduced aminoacid sequences of three OSCs from L cylindrica The sequence of the Luffa LcIMS1 ... to the sites of pYES2 (Invitrogen), to obtain pYES2-LcIMS1 in which the ORF of the cDNA was ligated to the GAL1 promoter in the sense orientation The nucleotide sequence of the inserted DNA was...

Ngày tải lên: 08/03/2014, 23:20

... the importin b binding (IBB) domain in its N-terminus and the C-terminal NLSbinding domain In the absence of importin b, an auto-inhibiting part of the IBB domain forms an intramolecular interaction ... content of basic amino acid residues such as lysine (K) and arginine (R), and (ii) the presence of conserved proline(s) (P) potentially involved in breaking secondary structural elements within the ... NLS-binding domain, preventing the association with NLS on the cargo protein Thus, the presence or absence of importin b regulates the NLS binding ability of importin a The relatively large NLS-binding...

Ngày tải lên: 15/03/2014, 11:20

... a boiling granular layer, problems of modeling of avalanching, etc In spite of the fact that the foundations of the theory have been laid even at the beginning of the development of continuum ... problems of transportation of granular materials of minerals industry and agriculture production, problems of design of storage bunkers and grain tanks, problems of design of chemical machines with ... the material contained herein Printed on acid-free paper Springer is part of Springer Science+Business Media (www.springer.com) Foreword The new monograph ‘‘Mathematical Modeling in Mechanics of...

Ngày tải lên: 16/03/2014, 22:20

... legend of Figure with mutations as indicated in panel B Plus signs above the lanes indicate binding mixtures containing 0.5 μg recombinant GST/WRKY28 Minus signs above the lanes indicate binding ... WRKY28 binding sites indicated against a grey background A schematic representation of the fragments tested in EMSAs for binding WRKY28 is given in Figure 5B Figure 5C shows the consensus binding ... recombinant protein The position of the protein-DNA complexes is indicated by an arrow Plus and minus signs in panel B indicate the relative abundance of the shifted probe Mutational Analysis of...

Ngày tải lên: 11/08/2014, 11:20

... phosphoprotein profiles in the course of long-term HL illumination of both the WT and DnaJ mutant plants Indeed, the changes in the phosphorylation pattern of thylakoid proteins revealed the capacity of ... responses of the DnaJ mutants At first, the H 2O levels in the leaves of the DnaJ mutants and WT were detected using DAB (diaminobenzidine) as a substrate Notably, the staining intensity and accordingly ... photosynthetic capacity of respective mutants, we next investigated whether the DnaJ proteins are involved in regulation of the stability of the photosynthetic pigment protein complexes in the thylakoid...

Ngày tải lên: 12/08/2014, 03:21

... in the case of N1 contains several residues involved in BH3-peptide binding like Leu30, Glu32 and Leu33 The C-terminus half of alpha-helix contains mostly hydrophobic residues and is buried in ... from K7 in that it cannot bind DDX3 Like A52, K7 binds the TRAF domain of TRAF6 [15] but our search did not find a canonical TRAF6-binding motif in its sequence It is striking how proteins of these ... response One of the C6 binding partners was programmed cell death interacting protein (PDCD6IP/ALIX), which has been involved in apoptosis regulation, cytokinesis and HIV-1 budding VACV C6 also interacted...

Ngày tải lên: 12/08/2014, 04:20

... Characterization of outer membrane proteins involved in the oxidation of sulfur in Thiobacillus spp In: A.E Torma, M.L Apel and C.L Brierley (Eds.), Biohydrometallurgical Technologies, Proceedings of an International ... or by a change in their size A study of restrictive samples of the chromosomal DNA showed that, in strains actively oxidizing Fe + in the presence of Zn (70 g/l), the gene of zinc tolerance was ... polymorphism of chromosomal DNA in different strains of T ferrooxidans was studied by Karavaiko et al [48] Individual patterns of chromosomal DNA restriction in these strains supported the assumption of...

Ngày tải lên: 21/12/2016, 10:34

... role of the middle (M) domain will be described in detail below The C domain of eRF1 interacts with the C domain of eRF3 [15–18], and the binding of both factors is essential for fast kinetics of ... coli in vivo can be explained by their competitive inhibition at the ribosome-binding site [32] Together, the M and C domains of human eRF1, in the absence of the N domain, are able to bind to ... the M domain participates in hydrolysis of the peptidyltRNA ester bond The aim of this work was to determine the structure and obtain dynamic information on the M domain of human eRF1 in solution,...

Ngày tải lên: 07/03/2014, 05:20

... change in the pKa value of the amino acid residue(s) involved in malate inhibition may occur as a result of interaction with different amino acid residues in the allosteric site The amino acid ... structure of each isoenzyme that are involved in fumarate activation (in yellow), CoA activation (underlined) and malate inhibition (in green) of the forward reaction are highlighted In addition, ... least in triplicate and the error bars indicate SD Significant inhibition (as indicated in Materials and methods): dark grey and single-hatched bars Significant activation (as indicated in Materials...

Ngày tải lên: 23/03/2014, 04:20

... ethanol-induced mucosal injury in the upper gastrointestinal tract leading to increase in the permeability of the gut mucosa to endotoxins [43, 44] These factors are involved in ethanol-induced ... NM_014393 ▲ 1.52 regulation of transcription protein binding double-stranded RNA binding DNA binding ▼ 5.02 protein binding NM_001149 NM_004242 ▲- increased ▼- decreased Validation of microarray results ... ANK3 Gene title cofactor of BRCA1 integrin, beta staufen, RNA binding protein, homolog (Drosophila) high mobility group nucleosomal binding domain ankyrin 3, node of Ranvier (ankyrin G) Signal Log...

Ngày tải lên: 31/10/2012, 15:28

... inhibit phase I and induce phase II carcinogen metabolizing enzymes that initiate carcinogenesis They also inhibit the promotion stage of carcinogenesis by inhibiting oxygen radical-forming enzymes, ... this kind of information are found in Ody (1993) and Moore (1995) 14.2 Inadequate Testing of Herbal Medicine Products Many medicinal herbs have not yet been subjected to testing in human clinical ... may increase the risk of bleeding Ginseng may intensify the effects of these drugs, causing an excessive decrease in blood sugar levels (hypoglycemia) Ginseng may intensify the side effects of...

Ngày tải lên: 25/10/2013, 05:20

... students’ interest in learning English In short, speaking like Finocchiaro (1973:174) by using songs “language learning can be combined with recreation or aesthetic appreciation for a change of pace in ... 10 According to Harmer, listening is special because listening comprehension is a process in which our mind has to complete instantly the process of receiving, processing, and interacting with ... failure in the examination of English That was the reason why the author would like to conduct this research of using songs as a type of supplementary material in teaching listening skill in order...

Ngày tải lên: 29/01/2014, 10:33

... suggesting that the heterologous expression of genes encoding metalloproteins can lead to incorrect metal ion incorporation This is observed in rubredoxin and desulforedoxins where zinc, instead of ... quantities of protein utilizing the principle of protein-dye binding Anal Biochem 72, 248–254 Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4 Nature ... not contain NrdI, as assessed by ESI-QTOF-MS In our view, C ammoniagenes restricts the incorporation of iron into R2F in vivo, even in the absence of manganese, and it is the availability of manganese...

Ngày tải lên: 15/02/2014, 01:20

... seen in the putative T-box DNA-binding domain of t-bet, the STAT protein interaction domain, STAT protein all-alpha domain, STAT protein DNA-binding domain and SH2 domain of stat6, and the zinc-finger ... implicated in other protein–protein interactions, a STAT protein DNA-binding domain and an SH2 domain, which binds phosphorylated tyrosine residues in the context of a longer peptide motif within a ... FOX proteins, a leucine zipper domain and a C2H2 zinc finger domain, both of which are thought to help mediate DNA binding and may be involved in the induction of dimerization [43] Each of these...

Ngày tải lên: 16/02/2014, 09:20

... Crassostrea gigas [5] Interestingly, this protein, named C gigas chitinase-like protein (CgClp1) was found to be involved in the control of growth and remodelling processes in a manner similar ... sequences of the catalytic domain of members of the GH18 family resulted in the amplification of an expected 147 bp sequence Cloning and sequencing of this fragment revealed an ORF showing amino acid ... transcripts during development and in adult tissues To gain insights into possible physiological functions of Cg-Clp2, determination of its tissue distribution and temporal pattern of expression during...

Ngày tải lên: 19/02/2014, 00:20

... relationship of all of these genes remains to be clarified Expression and purification of Sno1p and Snz1p In light of the similarity in the amino acid sequence of Sno1p to that of the glutaminase subunit of ... consistently pointed to the formation of glutamate, proving unambiguously that the complex did indeed hydrolyze glutamine efficiently Kinetics of the glutaminase reaction The kinetics of glutamine hydrolysis ... Glutamine was effective in protecting the enzyme from inactivation and its effect was again dose-dependent, suggesting that inhibition by DON occurs at the active site or the glutamine-binding...

Ngày tải lên: 19/02/2014, 12:20

... previously shown that proteinprotein interactions can result in information transfer, imprinting effects and can modify the regulatory properties of the enzymes involved in this complex [1620] GAPDH ... the binding of CP12 The presence of this region at the interface of GAPDH and CP12 could also explain the kinetic changes observed for the binding of the substrate when the wild-type recombinant ... quantities of protein utilizing the principle of protein-dye binding Anal Biochem 72, 248254 Sali, A & Blundell, T.L (1993) Comparative protein modelling by satisfaction of spatial restraints J Mol...

Ngày tải lên: 19/02/2014, 16:20