enumeration of very small embryonic like stem cells in peripheral blood
Báo cáo y học: "Preferential expression of potential markers for cancer stem cells in large cell neuroendocrine carcinoma of the lung. An FFPE proteomic study" pptx
Ngày tải lên :
10/08/2014, 09:22
... for errors in trypsin specificity Carbamidomethylation of cysteines was taken as fixed modifications, and methionine oxidation and formylation of lysine, arginine and N-terminal amino acids as ... supporting the proteomic results Notably, nine cases of LCNEC including four used for the proteomic experiments Nomura et al Journal of Clinical Bioinformatics 2011, 1:23 http://www.jclinbioinformatics.com/content/1/1/23 ... Poly(rC)-binding protein 4.31 3.79E-02 1.01 23 16 Nomura et al Journal of Clinical Bioinformatics 2011, 1:23 http://www.jclinbioinformatics.com/content/1/1/23 Page of 13 Table Significant changes in...
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Báo cáo y học: "Therapeutic potential of human umbilical cord mesenchymal stem cells in the treatment of rheumatoid arthritis" pps
Ngày tải lên :
12/08/2014, 15:22
... self-antigen-reactive T cells in vivo [38] The upregulation of IL-10 is in line with the induction of Tregs in vitro and in vivo in our study Moreover, in our experiments we did not find UC-MSCs in the joint of the ... differentiation of UC-MSCs staining for alkaline phosphatase Arrows indicate the accumulation of intracytoplasmic alkaline phosphatase of osteoblast Original magnification × 40 involved in the suppression of ... that administration of UC-MSCs attenuated systemic inflammation in CIA in mice UC-MSCs downregulated the production of the proinflammatory cytokines TNF-a, and IL-6 in vitro and in vivo In addition,...
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Báo cáo y học: "Decreased effector memory CD45RA+CD62L– CD8+ T cells and increased central memory CD45RA–CD62L+ CD8+ T cells in peripheral blood of rheumatoid arthritis patients" ppt
Ngày tải lên :
09/08/2014, 01:21
... differences in the differentiation of T cells in RA patients Decreases in some of the subpopulations in peripheral blood may indicate that there is a selective migration of these cells out of the peripheral ... control individuals or in patients with SLE Reduction in peripheral blood effector memory CD8+ T cells in RA may indicate an increase in the migration of these cells into sites of inflammation, and ... patients, indicating that this perturbation in homeostasis of T cells is a specific feature of RA Although the naïve/memory phenotype of T cells has previously been investigated in RA in numerous...
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Báo cáo y học: " Increased levels of (class switched) memory B cells in peripheral blood of current smokers" docx
Ngày tải lên :
12/08/2014, 14:20
... plots of B cells and memory B cells in peripheral blood Flow cytometry plots of B cells and memory B cells in peripheral blood A representative example of the difference in percentage of CD20+ B cells ... e r Figure Memory4B cells in peripheral blood Memory B cells in peripheral blood A) Percentages of memory B cells and class switched memory B cells (D) in peripheral blood of current smokers (closed ... Figure Regulatory T cells in peripheral blood Regulatory T cells in peripheral blood A) Foxp3 percentages of CD4+CD25+ T cells and C) CD4+CD25high T cells in peripheral blood of COPD patients (closed...
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Báo cáo y học: "Increased interleukin-23 receptor+ T cells in peripheral blood mononuclear cells of patients with systemic lupus erythematosu" doc
Ngày tải lên :
12/08/2014, 15:22
... T cells in CD4+ T cells Day3 *p =0.0197 *p =0.0219 In a (a) N %IL-17+ CD4+ T cells in CD4+ T cells Day0 IL-17+CD4+ Fold increase in the percentage of CD4+ IL-17+ T cells Page of 11 Figure Increased ... several types of cells, including CD4+ T cells (Th17), CD8+ T cells, CD3+ CD4- CD8- T cells, gδ T cells, NK cells and neutrophils [4] IL-17 was initially associated with a Th1-type proinflammatory ... IL-23 plays only a minor role in the differentiation of Th17 from naïve T cells, it is necessary for driving the expansion of Th17 cells and is involved in the pathology of various autoimmune...
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Báo cáo y học: "Identification and isolation of embryonic stem cells in reproductive endocrinology: theoretical protocols for conservation of human embryos derived from in vitro fertilization" ppsx
Ngày tải lên :
13/08/2014, 23:20
... The end of the beginning for pluripotent stem cells Nature 2001, 414:92-97 23 24 25 26 Doerflinger RM: The ethics of funding embryonic stem cell research: a Catholic viewpoint Kennedy Inst Ethics ... Foundation This type of federal research funding strategy involving existing embryonic stem cell lines is consistent with the President's belief in the fundamental value and sanctity of human life The ... destruction of additional human embryos Indeed, the embryos from which the existing ESCs originated have already been destroyed Federal funding of medical research on these existing stem cell lines...
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Effects of high glucose concentrations on the expression of genes involved in proliferation and cell fate specification of mouse embryonic neural stem cells
Ngày tải lên :
30/09/2015, 06:36
... function of kidney, manifested initially as a persistent elevation in urinary excretion of albumin and subsequently as an increase in excretion of protein, is often associated with the increased ... seven members in the Hes family While it is likely that many of these genes are involved in 15 Chapter 1: Introduction the timing of differentiation in a variety of tissues, two of them in particular, ... spite of improvement in the management of diabetic pregnancies in the past decades, congenital malformations including NTDs in infants of diabetic mothers remain two to three times higher than in...
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Báo cáo y học: "ntravenous transplantation of allogeneic bone marrow mesenchymal stem cells and its directional migration to the necrotic femoral head"
Ngày tải lên :
25/10/2012, 11:18
... efficacy of stem cell transplantation, committed stem cells are isolated and purified, and single lineage stem cell transplantation is then performed Deng et al applied intravenous infusion of MSCs in ... However, intravenous injection of stem cells as a therapeutic strategy is less investigated in the treatment of femoral head necrosis Safety is a critical concern of intravenous transplantation of ... the stem cell homing is executed in two ways: (1) Cell necrosis after trauma induces the release of a series of signal molecules, and stem cells are motivated and migrate into peripheral blood...
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Báo cáo hóa học: " Nutraceutical augmentation of circulating endothelial progenitor cells and hematopoietic stem cells in human subjects" potx
Ngày tải lên :
18/06/2014, 16:20
... These data suggest Stem- Kine supplementation increases circulation of cells capable of giving rise to hematopoieticlineage cells in vitro Stem- Kine augments circulation of cells with EPC phenotype ... a peak increase of approximately 53% in the number of CD34 expressing cells and and a 90% increase in CD133 cells in circulation Furthermore, a significant augmentation of cells possessing hematopoietic ... marrow injury conditions [43], subsequent studies have expanded this finding to situations of inflammation [44], and peripheral tissue injury [45-47] Hematopoietic stem cells are being increasingly...
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Báo cáo khoa học: "Transplantation of canine umbilical cord blood-derived mesenchymal stem cells in experimentally induced spinal cord injured dogs" pot
Ngày tải lên :
07/08/2014, 23:22
... ytisrevinU lanoitaN luoeS fo ’slaminA yrotarobaL fo esU dna eraC eht rof ediuG‘ eht fo senilediug eht ot gnidrocca demrofrep saw noitagitsevni sihT yduts yrujni droc lanips latnemirepxe eht rof desu ... erew sgod ehT ICS eht retfa keew a demrofrep saw sCSM-BCU eninac fo noitatnalpsnarT :FSCGhmr fo noitcejni dna sCSM-BCU eninac fo noitatnalpsnarT noitcejni eht rof noitulos enilas fo lµ 051 ni 601 ... ilumits cinegotim cificepsnon ro ralullec yb decudni noitarefilorp etycohpmyl-T sserppus sllec lamorts worram enob namuH MA innaiG ,S itnasirG ,P iccuettaM ,DP inognoL ,M isenaliM ,M ingaM ,C...
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Báo cáo khoa học: "Functional recovery and neural differentiation after transplantation of allogenic adipose-derived stem cells in a canine model of acute spinal cord injury" potx
Ngày tải lên :
07/08/2014, 23:22
... H3: Immunofluorescence staining Scale bars = 50 μm Transplantation of ASCs in spinal cord injury 281 Fig Immunofluorescence staining of ASC group; A1-A3: Glial fibrillary acidic protein (GFAP), ... droplets (Fig 1B) The colonies of ASCs were subjected to Alizarin red S staining weeks after the initiation of osteogenic differentiation Intense Alizarin red S staining of the colonies confirmed ... cells For group V, the injured site was exposed by dorsal laminectomy and 150 μL of PBS was injected into the spinal cord at locations to depths of mm using a 30 gauge needle (middle of the injury...
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Báo cáo y học: "Association of reduced heme oxygenase-1 with excessive Toll-like receptor 4 expression in peripheral blood mononuclear cells in Behçet''''s disease" potx
Ngày tải lên :
09/08/2014, 10:22
... selective knockdown of HO-1 expression by using specific small interfering RNA resulted in upregulation of synthesis of proinflammatory cytokines, including interleukin-6, interleukin-8 and TNF, which ... over-expressed TLR4 cause reduction in HO-1 expression in peripheral blood mononuclear cells (PBMC), resulting in an augmentation of inflammatory responses in BD Page of 10 (page number not for citation ... elevated soluble CD14 in plasma of BD patients may further facilitate LPS binding to TLR4 [36] Interestingly, LPS-induced lung injury in a mouse model was rescued by administration of an HO-1 adenovirus...
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Báo cáo y học: " Over-expression of HO-1 on mesenchymal stem cells promotes angiogenesis and improves myocardial function in infarcted myocardium" pdf
Ngày tải lên :
10/08/2014, 05:21
... intraperitoneal injection of the HO-1 inhibitor zinc-protoporphyrin (ZnPP, Porphyrin Products, Logan, UT, USA) at a concentration of 50 μmol/kg/day, starting two days before and continuing until days ... increase in angiogenesis[16] Adenovirusmediated HO-1 gene transfer into the ischemic hindlimb facilitated a significant recovery of blood flow in the hindlimb, and this effect was, at least in ... marrow cells were cultured at a density of × 10 cells/ cm in a-minimum essential medium (a-MEM, Gibco, USA) with 10% fetal bovine serum (FBS, Invitrogen, USA) and 100 μg/ml penicillinstreptomycin...
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Báo cáo y học: "Sphingosine-1-phosphate promotes the differentiation of human umbilical cord mesenchymal stem cells into cardiomyocytes under the designated culturing conditions" pdf
Ngày tải lên :
10/08/2014, 05:21
... http://www.jbiomedsci.com/content/18/1/37 Page of Figure Immunostaining of anti-a-actinin and anti-a MHC in cells at different time points of culturing A strong expression of both a-actinin and MHC proteins (A, B, D, E, G, ... fluorescent immunostaining of aactinin of cells from three groups, while, J-L shows the fluorescent immunostaining of MHC of cells from these groups after and 10 days’ culturing Cells from CMCM and ... her proofreading of the manuscript This work was supported by The Major Project of Department of Science &Technolgoy of Hainan Province, P R of China.(No 20061003) Author details Department of Neurology,...
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Báo cáo y học: " High efficient isolation and systematic identification of human adipose-derived mesenchymal stem cells." ppsx
Ngày tải lên :
10/08/2014, 10:20
... bubble-shaped oil red O-staining lipid droplets appeared in part of the cells (Figure 4A) The size of lipid droplets increased after two weeks, and most of the differentiated cells showed red lipid ... these cells These data will significantly contribute to tissue engineering by providing abundant seeding cells with high quality List of abbreviations ADSCs: adipose-derived mesenchymal stem cells; ... Laboratory of Pathobiology, Ministry of Education, Norman Bethune College of Medicine, Jilin University, Changchun, China 2Department of Pathophysiology, MuDanJiang Medical College, Hei Long Jiang, China...
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báo cáo khoa học: " Efficacy of Mesenchymal Stem Cells in Suppression of Hepatocarcinorigenesis in Rats: Possible Role of Wnt Signaling" doc
Ngày tải lên :
10/08/2014, 10:21
... of MSCs to cancer cells, nature of tumour cells and cancer stem cells, integrity of immune system, number of stem cell passages and site of injection; all can affect the outcome of MSCs use in ... tissues by assessing the gene expression profile of some of the Wnt signaling target genes:cyclin D, PCNA, survivin, b-catenin Methods Ninety albino female rats inbred strain (Cux1: HEL1) of matched ... regulation of the transcription factor b-catenin, a key component of the Wnt signaling pathway [58] In the normal state, the binding of members of a family of soluble cysteine-rich glycoprotein ligands,...
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báo cáo khoa học: " Embryonic stem cells in scaffold-free threedimensional cell culture: osteogenic differentiation and bone generation" ppt
Ngày tải lên :
11/08/2014, 20:21
... cultivation in the presence of osteoinductive stimuli Numerous living cells were detected in the mineralized centre of the spheres by means of toluidine blue staining (Figure 1) Generally, the mineralization ... detected crystal -like structures in the interior of the microspheres These ESC microspheres resemble aggregates consisting of preosteoblasts Our finding of a mineralization in microspheres of DAG-treated ... prominent in the centre of the sphere, as demonstrated in histological sections stained with Figure Micromasses consisting of embryonic stem cells were cultured with or without DAG and stained...
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Báo cáo y học: " Curcumin mediated suppression of nuclear factor-κB promotes chondrogenic differentiation of mesenchymal stem cells in a high-density co-culture microenvironment" pdf
Ngày tải lên :
12/08/2014, 14:22
... inhibition of IκB kinase Page of 15 [13,18] There is increasing interest in curcumin as a therapeutic option for OA and RA, with evidence that curcumin inhibits the IL-1β-induced activation of ... the action of pro-inflammatory cytokines in the joint thus disrupting the inflammatory cycle inhibit IL-1β induced activation of degradative pathways in MSCs It must be noted that in this study ... positive chondrogenic signaling However, interaction of curcumin and TGF-β signaling in chondrocytes is an interesting point and will require further detailed investigations In the present study we...
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Use of IPs cell derived neural stem cells as a cellular vehicle for glioma and breast cancer therapy
Ngày tải lên :
09/09/2015, 18:56
... Route of administration of stem cells One limiting variable in development of stem cell therapeutics is the identification of a delivery method that is effective and minimally invasive Intravenous ... immunological incompatibility between patient and donor ES cells in vitro reprogramming of mature mouse fibroblasts into pluripotent stem cells (induced pluripotent stem cells, iPS cells) was first ... cell Human embryonic stem cell-derived neural stem cell Herpes simplex virus thymidine kinase Interleukin Intraperitoneal human induced pluripotent stem cell human induced pluripotent stem cell...
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Applying macromolecular crowding to promote the expansion and adipogenic differentiation of human mesenchymal stem cells in vitro; an effect of matrix reciprocity
Ngày tải lên :
11/09/2015, 09:16
... 2001;Function and interactions of integrins Cell Tissue Res (3):285-298 41 Mould AP, Askari JA, Aota SI, et al 1997;Defining the topology of integrin alpha5beta1-fibronectin interactions using inhibitory ... colony-forming cell and the haemopoietic stem cell Blood Cells (12):7-25 32 Moore KA, Lemischka IR 2006 ;Stem cells and their niches Science (5769):1880-1885 33 Lemischka IR, Moore KA 2003 ;Stem cells: interactive ... 2007 ;In vitro enhancement of collagen matrix formation and crosslinking for applications in tissue engineering: a preliminary study Tissue Eng (2):385391 37 Qian L, Saltzman WM 2004;Improving...
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