enterotoxin producing staphlocococcus shigella yersinia vibrio aeromonas and plesiomonas

... theoretical pI was 9.61 for VsEndA and 8.62 for VcEndA Expression and purification From L Escherichia coli culture, a total of 24 and 50 mg pure recombinant VsEndA and VcEndA proteins, respectively, ... (St Louis, MO, USA) Phusion DNA polymerase from Finnzymes (Espoo, Finland) and Vent and Taq Endonuclease I from V salmonicida and V cholerae Table List of PCR primers Restriction sites are underlined ... PCRs Restriction enzymes NcoI and SalI were purchased from New England Biolabs (Ipswich, MA, USA), and T4 DNA ligase was purchased from Sigma-Aldrich DNaseAlertTM and RNaseAlertTM QC System kit...

Ngày tải lên: 19/02/2014, 05:20

... of the sequence and structural alignments of Vvn, VsEndA and VcEndA The selected residues 69 and 71 were nonconserved between VsEndA and VcEndA, located in the DNA binding region and close to the ... MT Jr, Zhang W & Anderson CF (1998) Analysis of effects of salts and uncharged solutes on protein and nucleic acid equilibria and processes: a practical guide to recognizing and interpreting ... charge and steric effects may alter substrate binding and salt sensitivity In this study, the effect of NaCl concentration on the kinetic constants and thermodynamic stability of VsEndA and VcEndA...

Ngày tải lên: 16/03/2014, 06:20

... enhances and complements, as needed, technology development and extension, market information and organisation, and government policy It is particularly useful in cases of simultaneous market and ... statistics and head- and tailgroup comparisons for key parameters; and to test ten hypotheses using both ANOVA treatment tests for the overall significance of regional area and membership status and ... market information, b) research and extension into new varieties and production techniques, c) government price and extension policies, and d) multiplication and deepening of farmer cooperations...

Ngày tải lên: 27/06/2014, 15:20

... samples Chloramphenicol and cycloheximide were used as specific growth inhibitors of bacteria and fungi, respectively (Anderson and Domsch, 1975; Newell et al., 1983; Fuhrman and McManus, 1984; Bjornsen, ... biomass and metabolic activity in the Table Potentials and contributions of bacterial and fungal denitrifications in tea soils, the submerged litter and sediment of an irrigation pond, and the ... mainly produced by denitrifying bacteria and fungi Table shows the bacterial, fungal and the total denitrification potentials at in situ pH and at 30 ℃, and the ratios of fungal denitrification...

Ngày tải lên: 05/09/2013, 08:40

... PAGE gel and Coomassie Blue staining, and the band corresponding to a modified OTUB1 (rectangle) was excised and digested with trypsin The peptide mixture was analysed by a nano-UPLC-QTOF tandem ... and OTUB1–probe adduct were visualized by immunoblotting using HA antibodies and quantified for two experiments (black and grey bars) The intensities of the corresponding bands were measured and ... increased Yersinia uptake We therefore tested whether YpkA and RhoA interact in vitro and whether this protein complex includes OTUB1 YpkA was immunoprecipitated and the presence of OTUB1 and RhoA...

Ngày tải lên: 16/02/2014, 15:20

... et al production in humans and primates [2,3] Staphylococcal enterotoxins are relatively heat stable [2], and ingestion of staphylococcal enterotoxins causes emesis and diarrhea [4] Staphylococcus ... & Kumagai K (2003) Concentrations and specific antibodies to staphylococcal enterotoxin- C and toxic shock syndrome toxin-1 in bovine mammary gland secretions, and inflammatory response to the intramammary ... levels of interleukin (IL)-1b and IL-6 in the SP began to rise at h, and reached their peak levels between 48 and 96 h Over the dose range of 0.2–5.0 ngÆmL)1 of SEC1, IL-1b and IL-6 in the SP displayed...

Ngày tải lên: 19/02/2014, 00:20

... regression plots, the Km and kcat values determined for the spectroscopic assay were 1.04 ± 0.10 mm and 5.78 ± 0.58 s)1, and for the LC-MS assay were 1.05 ± 0.03 mm and 5.73 ± 0.16 s)1 (Table ... Coomassie blue showed single bands for the wild-type and mutants D392N and E315M, migrating with an Mr of  63 000 (Fig 4A) In the case of the E315Q mutant, an additional faint band was also seen at an ... trimer and the pentamer compared to the dimer were 34% and 30% for the wildtype, but 66% and 47% for the D392N mutant (Fig 5B) A Oligosaccharide synthesis by chitinase A Protein expression and hydrolytic...

Ngày tải lên: 20/02/2014, 01:20

... Cost of Producing Ethanol from Corn Starch and Lignocellulosic Feedstocks A Joint Study Sponsored by: U.S Department of Agriculture and U.S Department of Energy Andrew McAloon, Frank Taylor, and ... and fed to the beer column Steam and cooling water for heating and cooling of the mash, whole beer, and whole stillage are conserved by the use of heat recovery exchangers Fermentor off-gas and ... which resulted in a better understanding for all involved and models that were easy to use and understand V.1 Starch Model V.1.1 Changes Made to the Starch Model and Other Work 1) Production capacity...

Ngày tải lên: 22/02/2014, 05:20

... on nuụi 2.4.4 Vi khun Vibrio 2.4.4.1 c im chung Vibrio l vi khun gram õm, dng que hoc cong (phy khun), cú kớch thc tng ng vi kớch thc ca ging Aeromonas vỡ Vibrio cng thuc h Vibrionaceae Di ng, ... P.L Conway and S Kjelleberg, 1992 Intestinal Colonization Potential of Turbot (Scophthalmus maximus)- and Dab (Limanda limanda)-Associated Bacteria with Inhibitory Effects against Vibrio anguillarum ... Aeromonas, Edwardsiella ictaluri, Pseudomonas v Vibrio trờn ng vt thy sn 2.4.1 Vi khun Aeromonas 2.4.1.1 c im chung Aeromonas l vi khun thuc h Vibrionaceae, l nhúm vi khun gram õm, hỡnh que cú...

Ngày tải lên: 22/02/2014, 13:17

... khuẩn Aeromonas spp Cần Thơ 22 4.2.2 Kết kháng sinh đồ vi khuẩn Aeromonas spp Trà Vinh 24 4.2.3 Kết kháng sinh đồ vi khuẩn Aeromonas spp Bến Tre 26 4.2.4 Kết kháng sinh đồ vi khuẩn Vibrio ... đến giống loại vi khuẩn Aeromonas spp, Vibrio spp môi trường nuôi cá tra (nước bùn) thâm canh Cần Thơ, Trà Vinh, Bến Tre Lập kháng sinh đồ hai loại vi khuẩn Aeromonas spp Vibrio spp Xác định giá ... đặc biệt rõ nét E coli, Salmonella, Shigella, Enterobactera, Citrobacter cầu ký trùng Eimeria … 2.3 Vi khuẩn Aeromonas spp Vibrio spp Theo Inglis et al., (1993) Vibrio spp giống vi khuẩn Gram (-),...

Ngày tải lên: 22/02/2014, 17:13

... on nuụi 2.4.4 Vi khun Vibrio 2.4.4.1 c im chung Vibrio l vi khun gram õm, dng que hoc cong (phy khun), cú kớch thc tng ng vi kớch thc ca ging Aeromonas vỡ Vibrio cng thuc h Vibrionaceae Di ng, ... P.L Conway and S Kjelleberg, 1992 Intestinal Colonization Potential of Turbot (Scophthalmus maximus)- and Dab (Limanda limanda)-Associated Bacteria with Inhibitory Effects against Vibrio anguillarum ... Aeromonas, Edwardsiella ictaluri, Pseudomonas v Vibrio trờn ng vt thy sn 2.4.1 Vi khun Aeromonas 2.4.1.1 c im chung Aeromonas l vi khun thuc h Vibrionaceae, l nhúm vi khun gram õm, hỡnh que cú...

Ngày tải lên: 10/03/2014, 23:20

... structural and sequence homology with EcCM, the active site residues of 90-MtCM can be identified, and are shown in Fig The striking difference between EcCM and 90MtCM is that the EcCM residues Ser84 and ... none of these residues is a candidate for performing the role of Gln88 in EcCM Of the other two structures, PfCM has the conserved Ser70 and Gln74, and TtCM has Ser81 and Glu85 There are two Protein ... (*EhCM) [16], *MtCM [5-7], and *YpCM; and AroH class CMs such as Bacillus subtilis (BsCM) [18]’ was corrected to ‘Erwinia herbicola CM (*EhCM) [16], *MtCM [5-7], and *YpCM; and AroH class CMs such...

Ngày tải lên: 17/03/2014, 17:20

... Cloning and DNA sequencing of the V alginolyticus pepD gene Multiple nucleic acid sequences of PepD from Vibrio parahaemolyticus RIMD 2210633 (BA000031), Vibrio vulnificus YJ016 (BA000037) and Vibrio ... protease inhibitors and the effects of metal ions were determined The PepD activity was tested at various pH values using citric acid (pH 4, and 6) and Tris–HCl (pH 6, 7, 7.4, 8.5, and 9.5) (Fig 4A) ... optimal activity in the range pH 7–7.4 and declined at more acidic and alkaline pH values PepD retained only 80% and 86% of its maximal activity at pH and 8.5, respectively The PepD activity...

Ngày tải lên: 23/03/2014, 06:20

... (formerly Vibrio) anguillarum and V ordalii [26,27] It should be noted that the structures of the CPS and O-chain polysaccharide of L anguillarum and V ordalii have recently been re-examined and that ... phenol solution (4 h, 22 °C) and harvested by centrifugation (yield 25 g, wet weight) Isolation and purification of CPS and LPS The cells were washed with 2.5% saline (w/v) and extracted by the method ... Sephadex G-100 and lyophilized (yield mg) NMR spectroscopy NMR spectra were performed on Varian INOVA 500 and 600 MHz spectrometers using standard software NMR measurements were made at 25 °C and 60...

Ngày tải lên: 23/03/2014, 13:20

... (Myaeng and Jeong, 1999; Oh and Choi, 2001) use the phonetic differences between conventional Korean words and loanwords However, these methods require manually tagged training corpora, and are ... they are in an existing dictionary, and extract the remaining words as candidate loanwords Third, we use our own handcrafted rules to extract loanwords from the candidate loanwords While the rule-based ... that are similar to the candidate loanwords Then, we compute the phonetic similarities between each candidate loanword and each retrieved Katakana word using DP matching, and select a pair whose...

Ngày tải lên: 23/03/2014, 18:20

... H2O2 and variable concentrations of Br– (B) PhoN-Sf in 300 mM Br– and variable concentrations of H2O2 (C) PhoN-Se in mM H2O2 and variable concentrations of Br– (D) PhoN-Se in 300 mM Br– and variable ... TGGAATATACATCAGCAGAA-3¢ and the reverse primer 5¢-CGCAAGCTTTCACCTTTCAGTAATT-3¢ (the NcoI and HindIII sites, respectively, are underlined) The PCR was performed using the ExpandTM High fidelity PCR ... annealing (30 s at 55 °C) and extension (1 at 72 °C) using a programmable heating block (Eppendorf Mastercycler 5330) The PCR product was restricted with NcoI and HindIII and cloned into the corresponding...

Ngày tải lên: 24/03/2014, 03:21

... Animal Welfare Act and other federal statutes and regulations relating to animals and experiments involving animals and adheres to principles stated in the Guide for the Care and Use of Laboratory ... (1, 5, 30 and 60 for protein extraction and h and 16 h for RNA extraction), PBMCs were incubated with an inhibitor of interest at room temperature for 30 at 20 lm, except SP600125 and SB203580, ... pinpoint the efficiency and the effectiveness of MK591 Experimental procedures Cells and cell cultures Effects of SEB on human PBMCs vitamin solution (Gibco BRL, Grand Island, NY, USA) The cultures...

Ngày tải lên: 30/03/2014, 04:20

... water and an amphiphilic phase was constituted in two ways: (a) by using Triton X-114, a nonionic detergent that forms a single phase mixture with water at °C and separates into a water-rich and ... wavelength of 295 nm, and the data were analyzed using the Stern–Volmer equation [23] F0/F ¼ + Ksv[Q] where F0 and F refer to the fluorescence intensities in the absence and presence of acrylamide, ... monomer and oligomers (A) The HlyA monomer (– -), the oligomer formed during storage of the toxin in water for 30 days (— – —), and the oligomer generated in PtdCho-cholesterol vesicles and delipidated...

Ngày tải lên: 31/03/2014, 09:20

... an index with values of )1, and +1, which corresponds to upfield, random coil and downfield, respectively This image was constructed using the VINCE software (The Rowland Institute for Science, ... Yop synthesis in Yersinia pseudotuberculosis Mol Microbiol 42, 1075–1094 15 Anderson, D.M., Ramamurthi, K.S., Tam, C & Schneewind, O (2002) YopD and LcrH regulate expression of Yersinia enterocolitica ... Wolf-Watz, H (1995) Functional conservation of the secretion and translocation machinery for virulence proteins of Yersiniae, Salmonellae and Shigellae EMBO J 14, 4187–4195 19 Frithz-Lindsten, E.,...

Ngày tải lên: 31/03/2014, 21:21