ensemble fret methods in studies of intrinsically disordered proteins
Báo cáo Y học: Binding of Thermomyces (Humicola) lanuginosa lipase to the mixed micelles of cis-parinaric acid/NaTDC Fluorescence resonance energy transfer and crystallographic study ppt
... remaining part of thsis moiety in a similar manner to the model of one of the alkyl chains in the TLL complex with di-dodecyl phosphatidylcholine [15] (not presented in Fig 6B for the sake of ... dashed lines indicate hydrogen bonds between OA and the protein (B) Comparisons between several ligand binding modes in the catalytic cleft of TLL The stereoview of OA binding modes in molecule ... micelles of cis-PnA/NaTDC The presence of mixed micelles of cis-PnA/NaTDC was also suggested by the increase in the fluorescence emission intensity of cis-PnA recorded with increasing amounts of NaTDC...
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Báo cáo khoa học: Development of a baculovirus-based fluorescence resonance energy transfer assay for measuring protein–protein interaction potx
... for investigating the molecular mechanism involved in protein–protein interaction and for screening novel molecules that might enhance or block the protein–protein interactions of molecules of interest ... sensitive method of visualization of FRET and assessment of protein–protein interactions It can potentially allow for studying the associations of molecules of any proteins of interest Acknowledgements ... level of recombinant protein expression, manipulating it by adjusting the multiplicity of infection (MOI) By combining the merits of both FRET and the baculovirus system, a new baculovirus-based FRET...
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Báo cáo sinh học: "Combined optical trapping and single-molecule fluorescence" doc
... pulling the bead from the center of the trap and thereby increasing the load until rupture (‘unzipping’) of the 15 base-pair duplex occurred Experiments were conducted in an assay medium consisting ... binding and stepping motion in motor proteins, or to probe molecular mechanisms underlying conformational changes We anticipate that this technique will have broad applicability to the study of ... Figure S1 A simplified diagram of the optical layout of our instrument (see also [4]) The inset is a cartoon showing a detail of the specimen in the region illuminated by the three lasers (not...
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Tài liệu Báo cáo khoa học: DNA strand exchange activity of rice recombinase OsDmc1 monitored by fluorescence resonance energy transfer and the role of ATP hydrolysis pptx
... function of the protein may be related to some critical steps in the conversion of inactive forms of protein to that of active forms It is interesting and intriguing to note that in the absence of ... the latter Interestingly, it appears that protein binding to ssDNA in the absence of any nucleotide cofactor results in attenuation of spontaneous annealing (compare line 1, Fig with line 2, Fig ... activity of OsDmc1 by FRET C Rajanikant et al ficant changes either in ATPase or in DNA binding domains of OsDmc1 [22] The only discernible change in OsDmc1 is an insertion of seven amino acid...
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Tài liệu Báo cáo khoa học: Constitutive oligomerization of human D2 dopamine receptors expressed in Spodoptera frugiperda 9 (Sf9 ) and in HEK293 cells Analysis using co-immunoprecipitation and time-resolved fluorescence resonance energy transfer pdf
... changes in the proteins rather than changes in protein– protein interaction A combination of several methods seems therefore important for the demonstration of GPCR oligomerization The D2 dopamine ... of signal Membranes containing the equivalent of 100 fmol of receptors (as labelled with [3H]spiperone) were incubated with 2.5 nM Eu3+-labelled anti-FLAG Ig, in a total volume of 500 lL of incubation ... binding of ligands to the D2 dopamine receptor may exhibit co-operativity, which can be accounted for in terms of interactions between binding sites in an oligomer There is also the question of...
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Báo cáo khoa học: Ternary complex formation of pVHL, elongin B and elongin C visualized in living cells by a fluorescence resonance energy transfer–fluorescence lifetime imaging microscopy technique docx
... change of elongin C induced by binding of elongin B Increased FRET signals between pVHL-cerulean and citrine-elongin C by coexpression of elongin B suggest that a conformation change of elongin C induced ... fluorescent proteins in the following four combinations: cerulean-elongin B and citrineelongin C; cerulean-elongin B and elongin C-citrine; elongin B-cerulean and elongin C-citrine; and elongin B-cerulean ... lifetimes of donor proteins using time-domain FLIM Using the FRET FLIM technique, we observed strong intermolecular FRET signals between elongin B and elongin C For stable binding of pVHL30 to elongin...
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Báo cáo khoa học: Dimerization of mammalian adenylate cyclases Functional, biochemical and ¯uorescence resonance energy transfer (FRET) studies pot
... calmodulin, binding to the C-terminus [22] Issues of speci®city of intermolecular interactions were addressed with AC5 or AC6, which are inhibitable by Ca2+, apparently independently of calmodulin ... to play a dominant role in the intermolecular interaction The more discerning technique of FRET analysis in live cells showed that in addition to interacting with Tm1 and traf®cking to the plasma ... Rescue of inactive mutants by half molecules of AC8 in vivo A corollary of the experiments described above involving dominant negative suppression of adenylate cyclase activity is the rescue of inactive,...
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SINGLE MOLECULE STUDY ON DNA PROTEIN INTERACTION IN PROKARYOTES AND EUKARYOTES
... without forming aggregates Many kinds of proteins are involved into the DNA compaction process Histone chaperons are a group of proteins in eukaryotic cells to help chromatin assembly The binding mechanisms ... concentration of the reaction buffer into infinite or to add certain amount of competing DNA, both of which are designed to obtain the optimal ratio of DNA to histone proteins As described in the previous ... regained great interests due to their functions in facilitating reconstitution of chromatin for functional and structural studies in vitro Histone chaperones are a group of proteins which can bind...
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Tài liệu Báo cáo khoa học: Loose interaction between glyceraldehyde-3-phosphate dehydrogenase and phosphoglycerate kinase revealed by fluorescence resonance energy transfer–fluorescence lifetime imaging microscopy in living cells doc
... fluorophores, being independent of the strength of protein–protein interactions In the case of very weak interactions as described in this article, rapid association and dissociation of FRET pairs may ... depend on the length of linkers In the linker containing seven amino acids, a Pro-Pro sequence that is not contained in other linkers is incorporated This less flexible structure may inhibit fluorophores ... because of its inherent instability Our FRET FLIM system may serve as a valuable tool for investigating weak interactions in the complex in living cells C-3¢, and pcerulean-C1 For the construction of...
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Báo cáo Y học: Probing intermolecular protein–protein interactions in the calcium-sensing receptor homodimer using bioluminescence resonance energy transfer (BRET) potx
... demonstrate agonist-induced alterations in BRET signal intensities reflecting 7TM dimer rearrangement as a result of the activating twist in the ATDs of the CaR homodimer Further investigations into the ... forms used in other studies [34,40] In the window of 500–530 nm the emission of Rluc/GFP2 transfected cells was 7.4 times higher than that of Rluc transfected cells (Fig 6A) Interestingly, the ... this BRET2 assay in contrast to the original BRET assay using Rluc/coelenterazine h [34,40], may hold some practical advantages for future studies of GFP fusion proteins As the obtained BRET signal...
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Báo cáo khoa học: Receptor- and calcium-dependent induced inositol 1,4,5-trisphosphate increases in PC12h cells as shown by fluorescence resonance energy transfer imaging pot
... following an increase in Ins(1,4,5)P3 This has been observed in Madin–Darby canine kidney (MDCK) cells, in which Ins(1,4,5)P3 injection caused the redistribution of fusion proteins, regardless of ... containing CCh, which induced a second calcium increase and a further increase in Ins(1,4,5)P3 (Fig 3Aa) Both of these calcium increases were accompanied by increased Ins(1,4,5)P3, indicating ... fusion proteins changes almost instantaneously following the disruption of PtdIns(4,5)P2 binding Therefore, the delay observed in our previous study indicates that the release of fusion proteins...
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FUNDAMENTALS of KINEMATICS and DYNAMICS
... type of constraining condition determines the number of degrees of freedom (DOF) a mechanism has If the constraining condition allows only one DOF between the two links, the corresponding joint ... tipping of the dump bed All the previous examples involved only links with two connections to other links Such links are called binary links In the example of Figure 1.6, in addition to binary links, ... its number of degrees of freedom This translates into a number of independent input motions leading to a single follower motion A single unconstrained link (Figure 1.7a) has three DOF in planar...
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Atomistic modeling of energetics and dynamics of diffusive and frictional phenomena in c60 graphene based systems
... dependence of kinetic nanofriction We use the Einstein’s theory of Brownian motion to analyze the surface diffusion of the C60 admolecule on graphene, and show that the decrease of kinetic nanofriction ... regimes of surface diffusion in C60/graphene system according to the effect of temperature and rotational degrees of freedom (DOFs) of the admolecule In the case of rotational C60 (the set of arrows ... determine the kinetic nanofriction of mobile adsorbates [72] The Einstein’s theory of Brownian motion, which is widely applied in microsystems, provides a link between the diffusion coefficient of...
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Study of pharmacokinetics of prenylflavonoids and dynamics of estrogen action in sera following ingestion of epimedium using validated, ultra sensitive cell based bioassays
... using lipofectamine Sixteen hours post-transfection, the cells were washed, trypsinized and split at ratio of in 10, in 100 and in 1000 into selective media containing 100 μg/mL of hygromycin ... contained 143.3 mg/g of icariin, 0.157 mg/g of icariside I, 18.68 mg/g of icariside II, 0.009 mg/g of icaritin, and 0.122 mg/g of desmethylicaritin, respectively Following Epimedium administration, ... human subject in this study consumed 500 mL of decoction containing icariin: 12.6 mg, icaritin: 0.75 mg, and desmethylicaritin: 0.19 mg A single dose of 50 g per subject was chosen according to calculations...
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Study of pharmacokinetics of prenylflavonoids and dynamics of estrogen action in sera following ingestion of epimedium using validated, ultra sensitive cell based bioassays 2
... Combinatorial effects of binary mixtures of estradiol with genistein and Epimedium prenylflavonoids To study combinatorial effects of flavonoids and estradiol, binary mixtures containing increasing ... cell line 101 Figure 17: ERα combinatorial effects of binary mixtures of estradiol with genistein and Epimedium prenylflavonoids ERα stable cells were incubated with increasing doses of genistein ... 18: ERβ combinatorial effects of binary mixtures of estradiol with genistein and Epimedium prenylflavonoids ERβ stable cells were incubated with increasing doses of genistein (A), icariin (B), icariside...
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Study of pharmacokinetics of prenylflavonoids and dynamics of estrogen action in sera following ingestion of epimedium using validated, ultra sensitive cell based bioassays 4
... derivatives, genistin and naringin not bind to the sex 201 hormone-binding globulin Hence, it is not likely that icaritin will bind to the sex hormone-binding globulin protein found in serum The late ... absence of serum, binding of quercetin to recombinant ERα was comparable to that of genistein and kaempferol (Maggiolini et al., 2004; Leung et al., 2004) The presence of serum reduced ERα binding of ... human serum albumin but not sex hormone-binding globulin, which would influence its bioavailability A large proportion of proteins found in serum are made up of albumin, which binds and transports...
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Study of pharmacokinetics of prenylflavonoids and dynamics of estrogen action in sera following ingestion of epimedium using validated, ultra sensitive cell based bioassays 5
... ascertain whether the ingestion of estrogenic prenylflavonoids from Epimedium for the purpose of maintaining bone health can increase risk of ERα-positive breast cancer Interestingly, icaritin, ... (-)-epigallocatechin-3-gallate significantly increased the biochanin A area under the plasma concentration versus time curve in rats in both intravenous and oral administration of biochanin A According to ... especially in terms of icariin but it had a lesser amount of icaritin - 143.3 mg/g icariin, 0.009 mg/g icaritin and 0.122 mg/g desmethylicaritin The amounts of icariside I and icarside II were determined...
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Study of pharmacokinetics of prenylflavonoids and dynamics of estrogen action in sera following ingestion of epimedium using validated, ultra sensitive cell based bioassays 6
... Ma, D.F., Qin, L.Q., Wang, P.Y and Katoh, R 2008 Soy isoflavone intake increases bone mineral density in the spine of menopausal women: meta-analysis of randomized controlled trials Clin Nutr 27(1):57–64 ... Pharmacokinetics and bioavailability of the bioflavonoid biochanin A: effects of quercetin and EGCG on biochanin A disposition in rats Mol Pharm 4(6):865-72 Mueller, S.O 2002 Overview of in vitro ... determination of serum estrogenic bioactivity in children J Clin Endocrinol Metab 87:791–797 Pasqualini, J.R 2005 Enzymes involved in the formation and transformation of steroid hormones in the...
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Application of formal and dynamic equivalence to the translation of bị and được into english
... the meaning of winning as in “được hay thua” (win or lose) In many cases, is replaced by thắng The second meaning of is related to positive modality as in the case of “được phúc” (find happiness) ... analysis of translation He introduces the concept of equivalence in difference He suggests three kinds of equivalence known as: - Intralingual ( within one language, i.e rewording or paraphrase) - Interlingual ... has to interpret the meaning of the original This can open the door to all sorts of errors, including misunderstanding, influence of the current culture and translator bias This last one often...
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Tìm thêm: xác định các mục tiêu của chương trình khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo gắn với các giáo trình cụ thể xác định thời lượng học về mặt lí thuyết và thực tế điều tra đối với đối tượng giảng viên và đối tượng quản lí khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu nội dung cụ thể cho từng kĩ năng ở từng cấp độ xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct mở máy động cơ rôto dây quấn các đặc tính của động cơ điện không đồng bộ hệ số công suất cosp fi p2 đặc tuyến hiệu suất h fi p2 đặc tuyến mômen quay m fi p2 đặc tuyến tốc độ rôto n fi p2 đặc tuyến dòng điện stato i1 fi p2 động cơ điện không đồng bộ một pha thông tin liên lạc và các dịch vụ từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng 9 tr 25