enhancement of gene transfection with ppaa
Tài liệu Báo cáo khoa học: The splicing factor ASF/SF2 is associated with TIA-1-related/ TIA-1-containing ribonucleoproteic complexes and contributes to post-transcriptional repression of gene expression doc
Ngày tải lên :
16/02/2014, 15:20
... buffer After h of binding at °C, beads were washed once with mL of CBB and once with modified CBB (0.02% NP40) Elution of the bound proteins was performed by addition of · 600 lL of calmodulin ... export, with this latter event relying on the RNA-binding activity of both RRMs Finally, the association of ASF ⁄ SF2 with TIAR led us to investigate its role on the expression of reporter gene ... dilutions of transfection pools Purification of TIAR-TAP complexes NIH 3T3 cells (6 · 108) stably transfected with the pcDNA3.1-TAP or with the pcDNA3.1-TIAR-TAP construct were lysed in 20 mL of lysis...
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Báo cáo khoa học: Enhancement of intracellular concentration and biological activity of PNA after conjugation with a cell-penetrating synthetic model peptide docx
Ngày tải lên :
23/03/2014, 13:20
... the beating rate of CM measured on day of culture without and with pretreatment with either 0.2 lM of I or II on day and after seeding The basal beating rate of the CM on day of culture was 191 ... the mean of 30–40 cells or cell cluster ± SEM Fig Influence of 10)5 M nociception on the beating rate of CM measured on day of culture without and with pretreatment with either 0.2 lM of II or ... regions of interest (16 · 16 pixel; 30 scans with a scan time of s with double averaging) in the cytosol and one in the nucleus of three selected cells were chosen such that the intensity of the...
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Báo cáo khoa học: On the reaction of D-amino acid oxidase with dioxygen: O2 diffusion pathways and enhancement of reactivity docx
Ngày tải lên :
28/03/2014, 23:20
... half-reaction of wild-type and variants of DAAO (A) Comparison of time courses of the (re)oxidation of reduced wild-type and W50 variants of DAAO followed at 455 nm upon mixing of 10 lM reduced enzyme with ... target for the study of O2 interactions The third such residue is I225, the side chain of which, together with the side chain of M213, forms a large part of the DAAO active site roof; it is located ... present study represents an extension of these previous studies with the goal of enhancing the reactivity of DAAO with dioxygen It is based on the predictions of the implicit ligand sampling analysis...
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Báo cáo khoa học: Synergistic co-operation of signal transducer and activator of transcription 5B with activator protein 1 in angiotensin II-induced angiotensinogen gene activation in vascular smooth muscle cells potx
Ngày tải lên :
30/03/2014, 02:20
... expression of the AGT gene in VSMCs stimulated with Ang II, the effect of Ang II on the phosphorylation of STAT5 and c-Jun was measured Ang II stimulated the phosphorylation of STAT5B and c-Jun, with ... immunoprecipitated with antibody to STAT5B, suggesting that STAT5B interacts with AP-1 Treatment of VSMCs with Ang II for and h resulted in an increase in the interaction of STAT5B with c-Jun The interaction of ... through enhancement of the phosphorylation and association to DNA of Jun proteins in the induction of the AGT gene by Ang II in VSMCs ChIP assays confirmed that Ang II increased the recruitment of...
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Báo cáo khoa học: Transfection with 4-hydroxynonenal-metabolizing glutathione S-transferase isozymes leads to phenotypic transformation and immortalization of adherent cells pdf
Ngày tải lên :
30/03/2014, 13:20
... specifically the effects of hGSTA4 transfection with the increased metabolism and depletion of 4-HNE, we investigated the effect of transfection with mutant hGSTA4 devoid of GST activity towards ... rate of the transformed cells Our studies are limited to evaluating the expression of only a few key genes known to be involved in cell-cycle events An assessment of the effect of transfection with ... enlargement of HLE B-3 cells weeks after transfection; (d) budding of rounded cells from giant cells weeks after transfection; (e) anchorage-independent growth of transformed rounded cells (B) Transfection...
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Báo cáo Y học: Interaction of the GTS1 gene product with glyceraldehyde3-phosphate dehydrogenase 1 required for the maintenance of the metabolic oscillations of the yeast Saccharomyces cerevisiae pdf
Ngày tải lên :
31/03/2014, 21:21
... promoters and 5¢ halves of the TDH genes The remaining fragments containing the 3¢ halves of the TDH genes were ligated with fragments from the plasmid ASAJ2682 containing the kan gene as a selective ... compared with the others (data not shown), the one with the C-terminal 97 residues (GAPDH3-C97) was used to search for binding site(s) of Gts1p The screening of a series of deletion mutants of GTS1 ... regulation of the ultradian oscillations in yeast, we constructed deletion mutants of each gene and subjected them to continuous culture As the deletion of TDH genes reportedly inhibits the growth of...
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Báo cáo sinh học: "Gene therapy with tumor-specific promoter mediated suicide gene plus IL-12 gene enhanced tumor inhibition and prolonged host survival in a murine model of Lewis lung carcinoma" pptx
Ngày tải lên :
18/06/2014, 19:20
... al Journal of Translational Medicine 2011, 9:39 http://www.translational-medicine.com/content/9/1/39 Page of 10 Figure General outline of the in vivo experimentation with mouse model of LLC ANOVA ... therapeutic efficiency of target suicide gene therapy mediated by hTERT promoter in combination with immuno -gene therapy in a murine model of LLC both in vitro and in vivo Xu et al Journal of Translational ... effectively cooperate with HRP/IAA gene therapy Another major focus of the present study was to evaluate whether suicide gene therapy plus immuno -gene therapy had any Page of 10 toxicity on the...
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Báo cáo hóa học: " The product of the Herpes simplex virus 1 UL7 gene interacts with a mitochondrial protein, adenine nucleotide translocator 2" pot
Ngày tải lên :
20/06/2014, 01:20
... proteins that interact with UL7 Mutagenesis of viral genomes in E coli and generation of recombinant viruses First, we generated a UL7 mutant virus genome (pMT101) in which a domain of UL7 encoding ... transfected with μg of pcDNA-MEF or pMEF7 per dish using FuGENE (Roche Applied Science) At 48 h post -transfection, cells were harvested, washed with phosphate-buffered saline (PBS), and lysed in ml of ... were transfected with pCMV(f)ANT in combination with pFLAG-CMV-2 or pCMV(f)7 using FuGENE At 48 h post -transfection, cells were harvested, washed with PBS, and lysed in 500 μl of NP40 buffer (50...
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báo cáo hóa học:" Gene therapy with tumor-specific promoter mediated suicide gene plus IL-12 gene enhanced tumor inhibition and prolonged host survival in a murine model of Lewis lung carcinoma" doc
Ngày tải lên :
20/06/2014, 03:20
... al Journal of Translational Medicine 2011, 9:39 http://www.translational-medicine.com/content/9/1/39 Page of 10 Figure General outline of the in vivo experimentation with mouse model of LLC ANOVA ... therapeutic efficiency of target suicide gene therapy mediated by hTERT promoter in combination with immuno -gene therapy in a murine model of LLC both in vitro and in vivo Xu et al Journal of Translational ... effectively cooperate with HRP/IAA gene therapy Another major focus of the present study was to evaluate whether suicide gene therapy plus immuno -gene therapy had any Page of 10 toxicity on the...
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Báo cáo hóa học: "Multicriteria Gene Screening for Analysis of Differential Expression with DNA Microarrays" potx
Ngày tải lên :
23/06/2014, 01:20
... DIFFERENTIAL EXPRESSION PROFILE EXPERIMENTS This type of experiment is very common in genetics research [19, 20] and involves comparing gene expression profiles of a set of G genes expressed in two ... relevant segments of these two ordered sequences of CIs Screening all genes with maximum lower endpoints > fcmin and minimum upper endpoints < −fcmin generates the set of declared positive genes Ᏻ2 ... two-stage algorithms with common Stage 1, which is to select the gene responses g ∈ Ᏻ1 that pass the paired-t test of hypotheses (1) with fcmin = at a FDR level of 20% The second stage of the “Two-stage...
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Báo cáo khoa học: " NÂNG CAO TRỊ SỐ OCTAN CỦA XĂNG MOGAS 90 BẰNG PHỤ GIA FERROCENE VÀ ETANOL ENHANCEMENT OF OCTAN NUMBER OF MOGAS 90 GASOLINE WITH FERROCENE AND ETANOL ADDITIVES " ppsx
Ngày tải lên :
22/07/2014, 20:20
... (2005), Xăng không chì - yêu c kỹ ầu thuật, Hà Nội [5] R.L.Furay (1985), “Volatility Characteristics of Gasoline – Alcohol and Gasoline Ether Fuel Blends” AE paper 852116 [6] T.V.Rasskazchikova, V.M.Kapustin, ... Additive to utomotive Gasolines Production and Use in Russia and Abroad” Chemistry & Technology of Fuels and Oils, Vol.40, No.4 95 ...
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Báo cáo khoa học: " Enhancement of protective immune responses by oral vaccination with Saccharomyces cerevisiae expressing recombinant Actinobacillus pleuropneumoniae ApxIA or ApxIIA in mice" pptx
Ngày tải lên :
07/08/2014, 20:23
... of 500 µl of 10 mM PBS (pH 7.2) and group B (vector) was orally vaccinated with 20 mg of S cerevisiae powder dissolved into 500 µl of 10 mM PBS (pH 7.2) The vaccinated groups were immunized with ... subcutaneously injected with 100 µg of protein extract after emulsifying with complete Freund's adjuvant (Sigma, USA) This was then followed by a boost immunization with the same amount of antigens after ... protective efficacy of our vaccine strategy by challenge with a field isolate of A pleuropneumoniae serotype Materials and Methods Preparation of vaccines The apxIA and apxIIA genes were cloned...
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Báo cáo y học: "Association of ENPP1 gene polymorphisms with hand osteoarthritis in a Chuvasha population" doc
Ngày tải lên :
09/08/2014, 06:23
... involvement of a particular gene In such a situation, the analysis of a rare monogenic disorder with an overlapping phenotype may give a clue to the right gene or pathway Recently, we identified the genetic ... identified when sequencing the ENPP1 gene in 23 unrelated patients with generalized arterial calcification of infancy, together with their parents and a number of control individuals [9,12] All ... association study with DNA markers of the ENPP1 locus We selected three STR and four SNP markers of the ENPP1 locus to obtain a fairly complete coverage of the gene region (Fig 2) Analysis of linkage...
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Báo cáo y học: "The protective effect of licofelone on experimental osteoarthritis is correlated with the downregulation of gene expression and protein synthesis of several major cartilage catabolic factors: MMP-13, cathepsin K and aggrecanase" docx
Ngày tải lên :
09/08/2014, 06:23
... molecules of the target gene to the number of molecules of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene The primer efficiencies for the test genes were the same as those for the GAPDH gene ... changes with a Mankin score of 5.1 (3–11) and a safraninO score of (0–4) Specimens from licofelone-treated groups had a Mankin score of 3.5 (0–10) and a safranin-O score of 0.4 (0–3) with the ... level of the enzyme was found to be significantly decreased by licofelone treatment at both the tested dosages 5-LOX gene expression and protein synthesis Although the level of gene expression of...
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Báo cáo y học: "Genetic polymorphisms in PTPN22, PADI-4, and CTLA-4 and risk for rheumatoid arthritis in two longitudinal cohort studies: evidence of gene-environment interactions with heavy cigarette smoking" potx
Ngày tải lên :
09/08/2014, 10:23
... presence of the PTPN22 risk allele, with a threefold elevated odds of developing RA in the presence of both factors Kallberg and colleagues [38] recently explored potential geneenvironment and gene- gene ... pack-years of smoking, for each of the genotypes Among those with the CC genotype of PTPN22, a modest effect of heavy smoking was observed (OR = 1.22 [95% CI = 0.81 to 1.83) However, among those with ... Replication of putative candidategene associations with rheumatoid arthritis in >4,000 samples from North America and Sweden: association of susceptibility with PTPN22, CTLA4, and PADI4 Am J Hum Genet...
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Báo cáo y học: "Testing for the association of the KIAA1109/Tenr/IL2/IL21 gene region with rheumatoid arthritis in a European family-based study" ppt
Ngày tải lên :
09/08/2014, 14:20
... Woolf's method [18], with Haldane's correction [19] Haplotypes were generated using GENEHUNTER software [20] We then carried out multi-locus tests of association testing haplotypes of SNPs using the ... haplotypes of the KIAA1109/Tenr/IL2/IL21 gene region These seven haplotypes of the combined five SNPs of the block have a pooled frequency of 96% We observed a nonsignificant undertransmission of the ... near the TNFα-induced protein gene (TNFAIP3) [8,9], and the signal transducer and activator of transcription (STAT4) gene [10,11] – a new genetic region associated with RA was described in the...
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báo cáo khoa học: "Induction of endogenous γ-globin gene expression with decoy oligonucleotide targeting Oct-1 transcription factor consensus sequence" pps
Ngày tải lên :
10/08/2014, 22:20
... expression of the endogenous γ-globin genes by competing with the Oct-1 consensus sequences of the endogenous γ-globin gene for the Oct-1 binding, resulting in activation of the endogenous γ-globin gene ... limited copy numbers of the target genes result in the requirement of much fewer molecules of decoy oligonucleotide for efficient regulation of transcription of the target genes Therefore, the ... our previous studies, mutations generated at the -280 Oct-1 consensus sequence of the γ-globin genes also resulted in increased transcription of the genes [14] All of these observations suggest...
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Báo cáo y học: " Concomitant homozygosity for the prothrombin gene variant with mild deficiency of antithrombin III in a patient with multiple hepatic infarctions: a case report" pdf
Ngày tải lên :
11/08/2014, 12:20
... An ultrasound scan of the patient's abdomen showed mild splenomegaly with a trace of ascites During a follow-up in clinic two months later, he complained of increased lethargy with reduced appetite ... life-long anticoagulation of heterozygous carriers of the prothrombin gene mutation after a single episode of DVT But in this case, there was limb DVT and a visceral thrombosis with secondary portal ... homozygosity for the prothrombin gene variant with mild deficiency of antithrombin III in a patient with multiple hepatic infarctions: a case report Journal of Medical Case Reports 2010, 4:122...
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báo cáo khoa học: "Predicting prognosis of breast cancer with gene signatures: are we lost in a sea of data?" docx
Ngày tải lên :
11/08/2014, 12:21
... performance of a multivariate model largely depends on the number of independent informative genes included in the model, the magnitude of differential expression of the informative genes and the ... levels of gene- expression change that may be required for a functional effect The level of expression change that results in a functional change may be different from gene to gene: for some genes ... usually combined and genes are added incrementally to increase the predictive performance However, because many of the genes are highly correlated with each other, adding genes lower on the list...
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Báo cáo y học: "Semen-mediated enhancement of HIV infection is donor-dependent and correlates with the levels of SEVI" docx
Ngày tải lên :
13/08/2014, 01:20
... containing the luciferase reporter gene in place of nef were generated by transient transfection of 293T cells Virus stocks were treated with the indicated concentrations of SE and used to infect PBMC ... http://www.retrovirology.com/content/7/1/55 Page of 12 Figure Effect of SE on HIV infection (A) Schema describing the experimental procedure (B) Effect of treatment of virus stocks with 90% (v/v) of SE on R5 HIV-1 infection ... Figure Effect of exposure times and pH values on SE mediated enhancement of HIV infection (A) Effect of pre-incubation time on SE-mediated HIV infection enhancement R5 HIV-1 was mixed with the indicated...
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