engineered scaffolds and matrices tailored biomaterials for adipose stem cell engineering

... ability) or the lower limb [23] Results were established as excellent for MSTS score > 90%, good for 80-90%, satisfactory for 60-80% and poor for ≤ 60% score We further downgraded patient’s result by ... continued for two months and then K wires were removed and a functional brace was applied thus allowing elbow mobilisation After months gentle active and assisted wrist exercises were started and gradually ... (42-86%) and the actual mean value for operated side was 29 kg as compared to 40 kg for contralateral normal side The average range of movements were 52° (35°-75°) forearm supination, 37° (20°-60°) forearm...

Ngày tải lên: 20/06/2014, 04:20

... ability) or the lower limb [23] Results were established as excellent for MSTS score > 90%, good for 80-90%, satisfactory for 60-80% and poor for ≤ 60% score We further downgraded patient’s result by ... continued for two months and then K wires were removed and a functional brace was applied thus allowing elbow mobilisation After months gentle active and assisted wrist exercises were started and gradually ... (42-86%) and the actual mean value for operated side was 29 kg as compared to 40 kg for contralateral normal side The average range of movements were 52° (35°-75°) forearm supination, 37° (20°-60°) forearm...

Ngày tải lên: 20/06/2014, 07:20

... silence and Cell growth arrest ALCL cells Figure Development of a tumor cell type-selective and cancer gene-specific nanocomplex for ALCL cells A, A nano-sized carrier core structure was initially formed ... repressive effects on tumor cells The use of specific ligands for cell targeting and reduction of the PEI dose is critical for in vivo feasibility PEI polymers have been used for cell transfection at ... for developing therapeutic agent by formulating a nanocomplex that is both tumor cell- selective and cancer gene-specific for ALCL The nanocomplexes are specific and noncytotoxic to lymphoma cells,...

Ngày tải lên: 11/08/2014, 00:22

... of recovery and optimize their self-management This research will also provide valuable information needed for the development and testing of interventions for patients at high risk for poor health ... screening and preventive practices for long-term survivors after hematopoietic cell transplantation: joint recommendations of the European Group for Blood and Marrow Transplantation, the Center for ... V, Lauson J: Development, feasibility and compliance of a web-based system for very frequent QOL and symptom home self-assessment after hematopoietic stem cell transplantation Qual Life Res 2005,...

Ngày tải lên: 20/06/2014, 15:20

... marker for human hematopoietic stem and progenitor cells Blood 1997, 90:5002-5012 22 Wu Y, Wu PY: CD133 as a marker for cancer stem cells: progresses and concerns Stem Cells Dev 2009, 18:1127-1134 ... cancer stem and progenitor cells Stem Cells Dev 2009, 18:17-25 Ginestier C, Hur MH, Charafe-Jauffret E, Monville F, Dutcher J, et al: ALDH1 is a marker of normal and malignant human mammary stem cells ... cancer stem cell; bFGF: basic fibroblast growth factor; FGFR: fibroblast growth factor receptor; HSC: hematopoietic stem cell; MSC: mesenchymal stem cell; ALDH: aldehyde dehydrogenase Stratford...

Ngày tải lên: 13/08/2014, 15:21

... transcriptional processes (for zinc ion binding and transcription) and apoptotic processes (for apoptosis, cell death, death, programmed cell death and regulation of apoptosis) (Table 1) Genes for transcriptional ... of the peripheral nervous system, such as Schwann and glia cells of the autonomic and enteric nervous systems, endocrine cells, such as the adrenal medulla, and C-cells of the thyroid as well ... these cells BMP2+ cells give rise to osteoblasts and express gene markers for satellite cells and fibroblasts Expression of osteopontin, Cbfa, osteocalcin and alkaline phosphatase in BMP2+ cells...

Ngày tải lên: 14/08/2014, 08:20

... biomaterials, for cartilage tissue engineering will be addressed 2.3.1 Stem cell based Approaches for Cartilage Tissue Engineering The aforementioned weaknesses of conventional clinical treatments and ... mesenchymal cells into precartilage condensations (Fig 5) Cellular condensation is dependent upon signals initiated by cell cell and cell matrix interactions and is associated with increased cell adhesion, ... cartilage to a range of mechanical loading such as compressive and shear force, and hydrostatic pressure, causing cell and tissue deformation and changes in fluid flow (Kock et al., 2012) Physiological...

Ngày tải lên: 09/09/2015, 08:12

... Characterization of Cell- Free Matrices To characterize cell- free matrices and identify components of the matrix 1.6.4 Propagation of hESCs on Cell- Free Matrices To cultivate hESCs on cell- free matrices ... staining for nuclei, ER and Factin in a typical morphology 3.3 Bioassembled cell- free matrices are complex mixtures of proteins 3.3.1 Col I Retention in cell- free matrices Cell layer samples and detergent-treated ... sites for integrins, also serves for cell attachment and the control of cellular behavior, such as cell migration and differentiation [62, 61] 41 LTBP-1 was found to be present in each of the matrices...

Ngày tải lên: 11/09/2015, 10:06

... analysis of DxSDOC and DxSDOCDOC matrices Mass spectrometry analysis was done in collaboration with Christopher Stephen Hughes and Prof Gilles Lajoie * Protein also found in GFR and standard Matrigel, ... Fibronectin*† 100.00% 21 14.60% Isoform of Fibronectin*† 100.00% 15 8.51% 240832 Isoform of Tenascin*† 100.00% 5.77% 100.00% 4.54% 77311 Isoform of Transglutaminase Isoform 2C2 of Collagen alpha-2(VI) ... 53635 152453 Isoform of Myosin9*† Isoform of FilaminA* 100.00% 2.81% 100.00% 1.10% Isoform of 100.00% 3.33% 47 IPI00220967, IPI00744135, IPI00856098 IPI00009865 58811 IPI00220327 Extracellular Matrix...

Ngày tải lên: 11/09/2015, 10:06

... binding and hence cell binding This contradiction could possibly be due to the increased presence of DCN and BGN in the DxSNP40/DNase and FcNP40/DNase matrices interfering with TGFB signaling for ... to FcNP40/DNase matrices either, and also settled on the bottom of the culture well in clusters that were easily moved (Figure 4B) Taken together, DxSNP40/DNase and FcNP40/DNase matrices, despite ... TGFB signaling for the hESCs, since DCN and BGN both bind active TGFB, and could lead to a decrease in the amount of active TGFB available for the hESCs 3.5 Matrices that maintain hESC pluripotency...

Ngày tải lên: 11/09/2015, 10:06

... incubated for weeks, after which teratomas from hESCs on DxSDOC and DxSDOCDOC were easily palpable The teratomas formed from hESCs cultured on DxSDOC and DxSDOCDOC matrices were harvested for formaldehyde ... were positive for SSEA-4, and 85% for hESCs on DxSDOC and 75.1% of hESCs on DxSDOCDOC TRA-160 markers expression was present in 52.4% of hESCs on Matrigel, 40.9% for hESCs on DxSDOC and 49.4% of ... expressed SSEA-3, with 88.9% and 88.7% for hESCs on DxSDOC and DxSDOCDOC respectively Similarly, 85.7% of hESCs on Matrigel expressed SSEA-4, with 84.2% and 88.1% for DxSDOC and DxSDOCDOC hESCs respectively...

Ngày tải lên: 11/09/2015, 10:07

... had cells positive for the pluripotency markers (Figure 6E-G) Control hESCs also mostly grew in colonies positive for the pluripotency markers, but there were some cells that were not positive for ... on DxSDOCDOC for 13 passages were induced into neural differentiation using a protocol modified from WiCell Research Institute, National Stem Cell Bank In this protocol, the hESCs formed embryoid ... or potency of stem cells in comparison to differentiated cells, DNA methylation studies were done The goal was to identify methylation changes between hES cells grown on the human matrices versus...

Ngày tải lên: 11/09/2015, 10:07

... tissue engineering, stem cell transplant therapy and also drug discovery, hESCs have to be maintained according to GMP standards, which require xeno-free, reproducible and scalable culture systems ... each human embryonic stem cell culture step that can contribute to a safer and more efficient human embryonic stem cell culture: For the enhancement of collagenous extracellular matrix, MMC theory ... produce a cell- free matrix for more efficient and safer human embryonic stem cell culture, five different matrices were produced by using different permutations of macromolecular crowders and detergent...

Ngày tải lên: 11/09/2015, 10:07

... Coatings for biomedical applications Edited by M Driver 38 Progenitor and stem cell technologies and therapies Edited by A Atala 39 Biomaterials for spinal surgery Edited by L Ambrosio and E Tanner ... biomaterials: structure and applications Edited by A Ruys 58 Standardisation in cell and tissue engineering: methods and protocols Edited by V Salih 59 Inhaler devices: fundamentals, design and ... Series in Biomaterials 15 Shape memory alloys for biomedical applications Edited by T Yoneyama and S Miyazaki 16 Cellular response to biomaterials Edited by L Di Silvio 17 Biomaterials for treating...

Ngày tải lên: 23/03/2014, 15:20

... forecasting and empirical research relating to financial engineering 2.9 Conclusion This chapter has presented a variety of networks for forecasting, for dimensionality reduction, and for discrete ... (1992); Lumsdaine and Papell (1997); Perron (1989); and Zivot and Andrews (1992)] Fortunately, for most financial time-series data such as share prices, nominal money supply, and gross domestic ... simply cut and paste the derivative formulae from this Toolbox to your own programs Simply type in the command funtool.m, and in the box beside “f=” type in the standard normal Gaussian formula,...

Ngày tải lên: 21/06/2014, 07:20

... particular point forecasts Granger and Jeon (2002) have suggested “thick modeling” as a strategy for neural networks, particularly for forecasting The idea is simple and straightforward We should ... overall network forecast They call this forecast a thick model forecast We can also use this method for obtaining intervals for our forecasts of the network Granger and Jeon have pointed out an intriguing ... where SK( ) and KR( ), for skewness and kurtosis, are defined as follows: SK( ) = T KR( ) = T T i − σ i − σ t=1 T t=1 (4.12) (4.13) while and σ represent the estimated mean and standard deviation...

Ngày tải lên: 21/06/2014, 07:20

... predictions each period and to use a trimmed mean of the multiple network forecasts for a thick model network forecast For comparing the linear and thick model network forecasts, the root mean ... also forecast out-of-sample well and make sense and add to our understanding of economic and financial markets 4.5.1 MATLAB Program Notes Many of the programs are available for web searches and ... different speeds and show different volatilities for the same sets of shocks and same laws of motion For values y0 = or y0 = 1, of course, the process remains at zero, and for y0 < and y0 > 1, the...

Ngày tải lên: 21/06/2014, 07:20

... option, τ ; and the annualized volatility or standard deviation of the underlying returns, σ The maturity parameter τ is set at unity for annual, 25 for quarterly, 125 for monthly, and 004 for daily ... chaos model), forecast onevar svjdmodel new1.m (for the stochastic volatility jump diffusion model), forecast onevar markovmodel new1.m (for the Markov regime switching model), and forecast onevar ... performance of alternative neural network models relative to the standard linear model for forecasting relatively complex artificially generated time series We see that relatively simple feedforward...

Ngày tải lên: 21/06/2014, 07:20

... draw upon the standard Phillips curve framework used by Stock and Watson (1999) for forecasting inflation in the United States They define the inflation as an h-period ahead forecast For our quarterly ... equivalent to a 12-month lag for monthly data, used by Stock and Watson (1999) for forecasting inflation To make the model operational for estimation, we specify the following linear and neural network ... curve is for the linear prediction errors, the dashed path is for the STRS prediction errors, and the dotted path is for the NNRS errors We see that the NNRS models outperforms both the STRS and linear...

Ngày tải lên: 21/06/2014, 07:20

... Poloni, and G Winter (eds.), Genetic Algorithms and Evolution Strategy in Engineering and Computer Science: Recent Advances and Industrial Applications West Sussex, England: John Wiley and Sons ... and nonlinear functions for extracting one component, brought in the next observation, and applied these coefficients and functions for estimating the new principal component We used this new forecast ... Theory and Practice Oxford: a Oxford University Press Baesens, Bart, Rudy Setiono, Christophe Mues, and Jan Vanthienen (2003), “Using Neural Network Rule Extraction and Decision Tables for Credit-Risk...

Ngày tải lên: 21/06/2014, 07:20