engineered particles without loss of activity
Báo cáo y học: " First Dominique Dormont international conference on "Host-pathogen interactions in chronic infections – viral and host determinants of HCV, HCMV, and HIV infections" pdf
... NK cells of HIV patients but increased in those of HCV patients The decrease in frequency of NK cells may be the consequence of a loss of IL15 expression, as the serum concentration of this cytokine ... steps: they prevent the infection of cells by HIV particles and aggregate gp41 at the plasma membrane of HIV-1-infected cells, resulting in the production of defective particles Unlike other gp41 epitopes, ... role of neutralizing antibodies in therapeutic resolution of acute HCV infection Longitudinal analysis of T-cell immune responses did not result in the identification of immune correlates of the...
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Báo cáo sinh học: "Molecular and cellular correlates of the CIITA-mediated inhibition of HTLV-2 Tax-2 transactivator function resulting in loss of viral replication" pot
... the basis of the functional inactivation of Tax-2 leading to the inhibition of HTLV-2 retrovirus replication Further studies of subcellular localization unveiled the co-localization of Tax-2 and ... the control activity of the pcDNA3 vector alone B)- CIITA did not affect basal promoter activity even after transfection of increasing amounts of CIITA plasmid The expression of recombinant fCIITA ... distribution of Tax-2 molecules was analyzed in the presence and in the absence of CIITA In the absence of CIITA, Tax-2 localizes both and in the cytoplasm and in the nucleus of 293T cells often with...
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Báo cáo hóa học: " Modulation of viral replication in macrophages persistently infected with the DA strain of Theiler''''s murine encephalomyelitis virus" docx
... investigate the effect of hemin on DRAW cells Treatment of DRAW macrophages with 65 μg/ml hemin resulted in an average fivefold increase of virus titers that was maintained until the end of the experiment ... pattern and whether this would be different from that of DRAW cells, we compared the cytokine profile of these cell lines Culture supernatants of macrophages, seeded at × 105 cells/well in 6-well ... http://www.virologyj.com/content/5/1/89 Figure Antiviral2effect of compounds in DRAW cells in function of time Antiviral effect of compounds in DRAW cells in function of time (A) mAb (1:10 dilution), ENV (0.316...
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o cáo hóa học:" Molecular and cellular correlates of the CIITA-mediated inhibition of HTLV-2 Tax-2 transactivator function resulting in loss of viral replication" potx
... the basis of the functional inactivation of Tax-2 leading to the inhibition of HTLV-2 retrovirus replication Further studies of subcellular localization unveiled the co-localization of Tax-2 and ... the control activity of the pcDNA3 vector alone B)- CIITA did not affect basal promoter activity even after transfection of increasing amounts of CIITA plasmid The expression of recombinant fCIITA ... distribution of Tax-2 molecules was analyzed in the presence and in the absence of CIITA In the absence of CIITA, Tax-2 localizes both and in the cytoplasm and in the nucleus of 293T cells often with...
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Báo cáo y học: "Control of viral replication after cessation of HAART" pdf
... Page of We thank the AIDS reference laboratory (ARL) of our institute for the collection and anonymisation of samples Author details Virology unit, Department of Microbiology, Institute of Tropical ... (UA); Faculty of Medicine and Pharmacy, Free University of Brussels (VUB) and Department of Gynecology and Obstetrics, Catholic University of Leuven (KUL), Belgium 5Department of Biomedical Sciences, ... that a total of 160 patients had stopped HAART for the first time, after a mean of 25 months of successful viral suppression Treatment was stopped under medical guidance, mostly because of toxicity...
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Báo cáo y học: " Management of HIV-1 associated hepatitis in patients with acquired immunodeficiency syndrome: role of a successful control of viral replication" ppsx
... liver disease without the identification of a distinct pathogen can complicate the clinical evolution of HIV-1 infection in children [11] Only a few cases of AIH, in which a role of HIV-1 as a ... onset of abnormal LFTs, after the exclusion of the most common causes of liver disease, it is necessary to assess the effectiveness of the current HAART and strongly evaluate the opportunity of ... after one month of therapy he showed an immune-virological recovery with a persistently improvement of LFTs (Figure 1) Conclusions Involvement of the liver during the course of HIV-1 disease...
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Báo cáo y học: " Dynamics of viral replication in blood and lymphoid tissues during SIVmac251 infection of macaques" docx
... expression of CD3 (II) CD4+ T cells were then defined based on expression of CD4 without expression of CD8 (III) Naïve CD4+ T cells were then separated based on expression of CD28 without expression of ... relevant differences in the ratio of 2LTR circle to total viral DNA levels in the different types of sample at any of the times studied, confirming the lack of accumulation of 2LTR circles Thus, in each ... dynamics of CD4+ T cells, viral replication and dissemination of the virus in the peripheral blood of SIV-infected macaques The dynamics of CD4+ T cells, viral replication and dissemination of the...
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Báo cáo y học: " Effective inhibition of HCMV UL49 gene expression and viral replication by oligonucleotide external guide sequences and RNase P" pptx
... Binding of EGSs to the target mRNA results in a helix structure of bp that is equivalent to the D stem of a tRNA, which usually has bp Our choice of a D stemequivalent structure of bp instead of bp ... expression of IE2, UL44, pp28, and gB (Table 1) The observed level of inhibition of UL49 expression was consistent with the extent of the reduction of viral growth HCMV is a member of the human ... sequence of 1713 nt In the absence of any EGS, no cleavage of the UL49 mRNA sequence was observed (Fig 2, lane 1) Efficient cleavage of this substrate by RNase P was observed in the presence of EGS324...
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Báo cáo y học: " Experimental depletion of CD8+ cells in acutely SIVagm-Infected African Green Monkeys results in increased viral replication" doc
... efficacy of mucosal transmission of SIVs in these species [1,11] Altogether, these characteristics define the paradox of SIV infection in natural hosts in which, in spite of low levels of cells ... A variety of markers of immune activation, including DR, CD25, CD69, and Ki67 was used to determine the levels of T cell immune activation during depletion of CD8+ cells The levels of activated ... the role of NK cells in HIV infections Even though our results show that depletion of CD8+ cells results in higher levels of viral replication during the Page of 13 acute SIVagm infection of AGMs,...
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Báo cáo y học: "Contribution of the C-terminal region within the catalytic core domain of HIV-1 integrase to yeast lethality, chromatin binding and viral replication" doc
... underlying this loss of lethality remained unknown A recent study has demonstrated that the expression of IN in yeast could catalyze the integration of DNA containing the LTRs of HIV into the ... yeast strain is independent of its catalytic activity HIV-1 IN-induced lethal phenotype in HP16 yeast strain is independent of its catalytic activity A The effect of IN expression on yeast growth ... well as to determine the importance of the C-terminal region of the catalytic core domain of HIV-1 IN in the viral life cycle, we have generated a series of IN mutations These mutants allowed...
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Báo cáo y học: "Apoptosis resistance in HIV-1 persistently-infected cells is independent of active viral replication and involves modulation of the apoptotic mitochondrial pathway" pot
... frequency of apoptosis when cells were treated with 10 μM H2O2 (Figure 2B) However, a higher concentration of H2O2 (50 μM) was capable of inducing 34% of annexin-V+ U937 cells, and only 16.5% of dead ... in H9/HTLVIIIB cells, p24 antigen, viral load and production of infectious viral particles were quantified The magnitude of decrease of p24 antigen production observed was 80% (119 ng/ml), 75% ... control of viral persistence, the mechanisms responsible of apoptosis resistance of persistently-infected cells are not well understood In particular, it is still unclear whether resistance of infected...
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Role of pentraxin 3 in shaping arthritogenic alphaviral disease from enhanced viral replication to immunomodulation
... adsorption, the detection of viral antigens present at hpi is indicative of binding and entry, and hpi is indicative of the synthesis of new virus particles Detection of intracellular viral antigens ... enhancement of RRV infection However, removal of the C-terminal domain did affect the ability of the N-terminal domain of PTX3 to modulate viral replication, resulting in only partial enhancement of viral ... Pathogenic Role of PTX3 in Alphaviral Infection release of PTX3 enables cells of monocyte-macrophage lineage to recognize and opsonize the pathogen, presenting it to activated phagocytic cells of the...
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Tài liệu Báo cáo khoa học: Minor capsid proteins of mouse polyomavirus are inducers of apoptosis when produced individually but are only moderate contributors to cell death during the late phase of viral infection ppt
... levels of apoptotic markers during the late stages of MPyV infection However, comparison of the levels of apoptotic markers and cytotoxicity during the replication of wt MPyV, and replication of ... Recently, viroporins of RNA viruses (such as 6K protein of Sindbis virus, E protein of mouse hepatitis virus, M2 protein from influenza A, 2B and 3A proteins of poliovirus, or p7 and NS4A of hepatitis ... observed at times which coincide with the production of high levels of the structural proteins Studies on the mechanism of the cytotoxic effect of MPyV infection show predominant necrosis (40–46%...
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Báo cáo khoa học: Fluorescence studies of the replication initiator protein RepA in complex with operator and iteron sequences and free in solution pdf
... better dened set of tting parameters The adequacy of the analyses was determined from the reduced weighted sum of squares of residuals, and visual inspection of the distribution of weighted residuals ... 1DR-short In the presence of excess 1DR, monomerization of RepA takes place Discussion One of the striking properties of RepA is that it is able to recognize two types of DNA sequence, either the ... disregard of uncertainties in the degree of labeling Because of signicant static quenching of the W94 donor upon complex formation, a correction was necessary to account for the fraction (d+) of uorescent...
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Báo cáo khoa học: Origins of DNA replication in the three domains of life pdf
... by unwinding of this intrinsically less stable region of duplex [6] A further level of complexity arises from the fact that DnaA is a member of the AAA+ family of ATPases This class of protein ... [14] Rather the role of ATP in the reaction is to serve as a switch that controls the activity of the helicase The ATP bound form of DnaC severely inhibits the helicase activity of DnaB and also ... position of the origin of replication in Pyrococcus, providing the first experimental proof of a localized origin of replication in the archaea [52] Interestingly, in a situation reminiscent of that...
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Báo cáo khoa học: The association of viral proteins with host cell dynein components during virus infection pdf
... phosphoprotein of rhabdovirus is a cofactor of the RNA polymerase complex and, in fact, facilitates the binding of the polymerase to the N-RNA complex [67] Using the SAD-D29 low-virulent strain of RV, ... traffic of viral particles towards the nucleus but also by the fact that microinjection of anti-dynein antibodies reduced the nuclear accumulation of viral capsids and immunoprecipitation of dynein ... KSTQT and KSIQI in the case of the P protein of rabies and Mokola viruses respectively [70,71] and SQTQT in the case of protein VP35 of ebolavirus [103] The presence of these sequences that closely...
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Báo cáo khoa học: The C-terminus of viral vascular endothelial growth factor-E partially blocks binding to VEGF receptor-1 docx
... lack of this groove may prevent viral VEGFs effectively binding the domain 2–3 linker region of VEGFR-1 In support of this model, changing Arg46 to Iso within loop of the groove region of ORFVNZ2VEGF ... binding of VEGFR-1 to VEGF-A from a concentration of 1.1 lgÆmL)1 (P £ 0.05; Fig 3C) Preincubation of soluble VEGF-A, VEGF-A-NZ2C, ORFVNZ2VEGF or ORFVNZ2VEGF-DC significantly inhibited the binding of ... made use of BaF3 cell lines expressing chimeric receptors consisting of the extracellular domain of either human VEGFR-1 or murine VEGFR-2 and the transmembrane and cytoplasmic domains of the erythropoietin...
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VIRAL REPLICATION pot
... F.V and R.M Krug (1991) Regulation of the extent of splicing of influenza virus NS1 mRNA: role of the rates of splicing and of the nucleocytoplasmic transport of NS1 mRNA Mol Cell Biol 11(2): 1092-8 ... involves the activity of M2 [15], and the infectious viral particles are released into the extracellular environment thanks to the activity of NA, which cleaves sialic acid molecules off the cell ... contains two NLS, both of which are of great relevance for proper nuclear import of vRNPs, since mutating either of the two NLS domains results in decreased accumulation of vRNPs within the nucleus...
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The Dynamics of Viral Marketing ∗ pot
... Probability of Credit Probability of Credit 0.15 0.05 80 0.25 Probability of Credit Number of Purchases 0.3 Probability of Credit 0.4 0.2 Number of Purchases Number of Purchases Number of Purchases ... predictive of number of senders ns Similarly, the number of edges e is predictive of number of senders ns Interestingly, price p is only related to the number of reviews v Number of recommendations ... success rate, n: number of nodes, ns : number of senders of recommendations, nr : log number of receivers, r: number of recommendations, e: number of edges, p: price, v: number of reviews, t: average...
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THE MECHANISMS OF DNA REPLICATION pot
... fundamental part of the life cycle of all organisms Not surprisingly many aspects of this process display profound conservation across organisms in all domains of life Successful duplication of the genetic ... degree of condensation and modification state of the proteins Not surprisingly the state of the chro‐ matin has significant effects on the replication of the DNA, influencing the selection of start ... function The ends or telomeres of eukaryotic chromosomes are particularly interesting as they present a problem of how to fully replicate both strands without a loss of genetic information The end...
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