endogenous and exogenous pectic enzymes in fruit and vegetable processing

... thermophilic proteins contain more arginines on the protein surface than mesophilic proteins [36–38] The effect of the large-scale increase in the number of arginines was tested experimentally in T reesei ... b-strand B3 and C-terminal b-strand A4, which most likely stabilize the structure The C-terminal b-strand A4 gives additional hydrogen bonding with b-strand A5 and the extension of b-strand B3 interacts ... finding in xylanases is that the increase in the Thr : Ser ratio in b-strands (Table 5) improves the b-forming propensities Over half of the residues in the family 11 xylanases are located in...

Ngày tải lên: 20/02/2014, 23:20

... Restraints - 23 Introductory Tutorial for SAP2000 Version B Click on the Assign menu > Joint > Restraints command to bring up the Joint Restraints form as shown in Figure 23 Figure 23 Joint Restraints ... lower left-hand corner of the interface should indicate “2 Points selected” E Click on the Assign menu > Joint > Restraints command to bring up the Joint Restraints form D Click on the pinned button ... and page 2-14 for setting the view) This view should be in the left window Also check that the Snap to Points and Grid Intersections command is active This will assist in accurately positioning...

Ngày tải lên: 06/09/2012, 15:56

... structure Zooming in and out may be performed in predefined increments Also, zoom in on a part of the structure using the mouse by dragging a window around the area of interest while holding down ... SAP2000 Getting Started Main Window Figure 4-1 shows the main window for the graphical user interface This window may be moved, resized, maximized, minimized, or closed using standard Windows operations ... maintaining a detailed log of the project; and a facility for running the analyses of multiple models in batch mode File Operations 4-9 SAP2000 Getting Started Defining Named Entities Defining...

Ngày tải lên: 01/04/2014, 03:20

... domain position in CDase is a packing artefact caused by domain swapping during crystallization because the interface between the N-terminal domain and the protein remainder (domains A and B) ˚ amounts ... indirectly involved in substrate binding Ca-I is missing in the three CD-degrading enzymes TVA-II, ThMA and BaCD The second and third calcium-binding sites of family no 13 enzymes show greater variation ... also present in the CD-producing CGTase [38] and in the CD-degrading TVAII [13], but not in the CD-degrading enzymes ThMA and BaCD nor in the majority of a-amylases Ca-II is located in the loop...

Ngày tải lên: 08/03/2014, 02:20

... additional binding subsite [17] Three arginine residues in the product sites of 1CEL (+1, +2 and +3) are proposed to assist in the binding and positioning of the substrate and play a role in the recognition ... reducing end of the cellulose chain Arginine side-chains are present in all equivalent locations in 1Q9H (Fig 7) Tunnel-forming loops There are four major loops involved in the cellulose-binding ... [44] and A aculeatus [23], however, contain a modular structure composed of a Cterminal carbohydrate-binding module linked via a proline/ serine/threonine-rich linker to the catalytic domain [45]...

Ngày tải lên: 23/03/2014, 13:20

... suggesting that the loops connecting, respectively, M5 and M6, and M6 and M7, would be equally short in both enzymes The region encompassing M8, M9 and M10 were aligned manually by aligning the ... assemble to form a binding pocket surrounding two Ca2+ ions A comparison of this binding pocket with the corresponding domain of our 3D model of PMA1_NEUCR is shown in Fig Ion binding site I Five ... Val289 and Ile293 in the N-terminal end of M3, and Trp756 and Gly757 in the cytoplasmic loop connecting M6 and M7 As shown by mutagenesis in yeast, mutation of Val289 to Phe resulted in an altered...

Ngày tải lên: 23/03/2014, 21:20

... near-field measurements in the range of – 200 nm, where the interference is not constructive The black and blue lines in Figs and are F-z curve and NSOM signal, respectively, measured in retraction direction ... D Tsai, and Y Lu, “Tapping-mode tuning fork force sensing for near-field scanning optical microscopy,” Appl Phys Lett 73(19), 2724 (1998) 16 B Hecht, H Bielefeldt, Y Inouye, D Pohl, and L Novotny, ... 260 nm and a gap of about 62 nm at the fused silica/aluminum interface and 134 nm at the top of the film The illumination is a λ = 457 nm plane-wave Experimental results and discussions In the...

Ngày tải lên: 06/05/2014, 08:54

... near-field measurements in the range of – 200 nm, where the interference is not constructive The black and blue lines in Figs and are F-z curve and NSOM signal, respectively, measured in retraction direction ... D Tsai, and Y Lu, “Tapping-mode tuning fork force sensing for near-field scanning optical microscopy,” Appl Phys Lett 73(19), 2724 (1998) 16 B Hecht, H Bielefeldt, Y Inouye, D Pohl, and L Novotny, ... 260 nm and a gap of about 62 nm at the fused silica/aluminum interface and 134 nm at the top of the film The illumination is a λ = 457 nm plane-wave Experimental results and discussions In the...

Ngày tải lên: 06/05/2014, 08:58

... integer n and for any principal polyomino c in Mn , there exists an increasing sequence c0 , · · · , cn of principal polyominoes such that c0 = ∅, cn = c Proposition 2.20 The standard polyominoes ... far through the standard polyominoes: for any integers m ≤ n and for any standard polyomino c in Sm , there exists an increasing sequence c0 , · · · , cn of standard polyominoes such that c0 ... , obtaining a polyomino c6 (this amounts to apply the slice–remodelling) We apply the trividing to c6 and obtain c7 = trividing(c6 ) We end the sequence by a filling filling(e3 ): c8 = filling(e3...

Ngày tải lên: 07/08/2014, 06:20

... provided, owing to the lack of simple and user-friendly software Quantitative data reflecting changes in nuclear shape and volume before, during and after cell insults and pharmacological interventions ... (37°C, 5% CO2) in RPMI 1640 medium containing 10% fœtal bovine serum (FBS) (Gibco, Burlington, ON, Canada) RPMI 1640 medium was phenol-red free and contained 1% penicillin-streptomycin For spectrofluorometric ... browsers, allow users to interact with the 3-D model Basic interactions include rotating and zooming, which allow the model to be inspected from virtually any viewpoint More advanced interactions are...

Ngày tải lên: 11/08/2014, 00:22

... is the binding energy between ligands and receptors This energy refers to the amount of ligand-receptor interaction and the diffusion kinetics for the recruitment of receptors to the binding site ... AuNPs falls in the range of 40-60 nm for reasonable values of membrane bending rigidity and ligand-receptor binding energy In the optical scattering study of AuNPs and cells, we investigated ... vesicle size are most in submicron scale and the AuNPs in the vesicle are in aggregated form To find the relation between the scattering optical intensity and number of AuNPs in the vesicle We prepared...

Ngày tải lên: 11/08/2014, 00:22

... Bad Krozingen, Südring 15, 79189 Bad Krozingen, Germany Authors’ contributions PM analyzed and interpreted patient data regarding the cardiac disease and was a major contributor in writing the ... manuscript and revising it critically for important intellectual content All authors read and approved the final manuscript Competing interests The authors declare that they have no competing interests ... thrombus Overlapping with the heparin therapy, we initiated oral anticoagulant therapy and continued with an International Normalized Ratio (INR) 2.0 - 3.0 He had an uneventful recovery and follow-up...

Ngày tải lên: 11/08/2014, 02:22

... defined by Shrout and Fleiss (ICC ≥ 0.75) [22] and in the almost perfect range as suggested by Dunn (ICC > 0.80) [23] Table provides SEMs, intra- and inter-examiner correlation coefficients, and ... day, inherent errors due to placing markers from palpation of boney landmarks [26], errors involved in the choosing of sixteen points on the photographs via the computer mouse by each examiner, and ... each of examiners, time, and participants was a fixed or random factor and (b) the type of error included (true score variance, systematic and/ or random) [28] In the equation for calculating ICCs,...

Ngày tải lên: 13/08/2014, 14:20

... family of signaling proteins Upon agonist binding to β receptors, a signaling cascade is launched involving the heterotrimeric G protein Gq, which activates phospholipase C, increasing inositol trisphosphate ... pixels containing tyrosine hydroxylase divided by the number of pixels containing sarcomeric α-actinin Metamorph version 7.1.0.0 was used for image processing E EGFP expression in intracardiac ... of injury[10] Second, instillation of a tetrodotoxin-containing solution into the pericardial sac acutely interrupts sympathetic responses of the heart[11] Third, myocardial infarctions involving...

Ngày tải lên: 24/08/2014, 12:57

... and redo Interactive Modeling Convenient Viewing Options Multiple orthographic and perspective views • Zooming and panning • Standard viewing angles • SAP2000 • User defined and named views Interactive ... Modeling Guides and Grids • Accurate dimensioning with guidelines and snapping SAP2000 • Multiple rectangular and cylindrical coordinate systems with flexible grid syst ems Interactive Modeling ... Duplicate and generate elements with move, merge, mirror and repl icate Interactive Modeling SAP2000 • Powerful Editing Tools Convenient dividing and meshing of elements Powerful Editing Tools Interactive...

Ngày tải lên: 11/06/2014, 03:16

... for fully nonlinear liquid sloshing in a tank On the other hand, Li & Fleming (1997) solved the Laplace equation in σ-coordinate with the use of FDM Frandsen & Borthwick (2003) and Frandsen (2004) ... results (dotted line), another NSE solver (Lin & Li, 2002) (dashed line), the Boussinesq equation solver (Lin & Man, 2007) (dash-dot line) and linear analytical solution (solid line) for H0 /h0 ... νg in gas and ν = νl in liquid) being the kinematic viscosity and ∂ui ∂uj + ), σij = ( ∂xj ∂xi (2.3) CHAPTER MATHEMATICAL FORMULATION OF NUMERICAL MODEL the rate of the strain tensor Initial and...

Ngày tải lên: 13/09/2015, 20:56

... maintain or improve tissue function [3] By combining life sciences, engineering principles and innovation, tissue engineering holds enormous potential in confronting the supply crisis by providing ... osteopontin and osteocalcin were present in PCL/Col group with mineralization at 28 days Marginal expression of collagen type I and osteopontin was observed on PCL although osteocalcin and mineralization ... proteins were retained in this manner indicating minimal processing damage Investigations into the performance of this novel hybrid mesh as a three-dimensional (3D), bio-functional tissue engineering...

Ngày tải lên: 14/09/2015, 08:24

... promising route to produce free-standing strings is to form metal interconnecting rods that mechanically bind the strings together, providing both strength and flexibility during application Interconnections ... by a solid line representing an S in one row and a dashed line representing an oppositely oriented S-structure in an adjacent row I1 and I2 represent the induced currents flowing in each half ... one in which the Sstrings are solely supported by interconnecting transverse rods and one in which the Sstrings are simultaneously held together by transverse interconnecting rods and a gold window...

Ngày tải lên: 06/10/2015, 20:42

... his M Sc degree in Physics from Utkal University, Orissa (India) in 1982 and obtained his Ph D degree in Physics from the same University in 2002 He served as a Faculty of Physics in Nayagarh (Autonomous) ... Orissa (India) from 1982-2004 and presently working as the Head of the faculty of Physics in KBDAV College, Nirakarpur, Orissa (India) since 2004 He has 27 years of teaching experience and 10 ... consider three equations (30), (32) and (33) being independent of the main flow component u1 and the temperature field θ1 We assume v1, w1 and p1 of the following form: v1 ( y , z ) = v11 ( y )...

Ngày tải lên: 05/09/2013, 14:58