embryonic stem cell derivation from human embryos
Human embryonic stem cell derived neural stem cells derivation, differentiation and MicroRNA regulation
Ngày tải lên :
10/09/2015, 09:08
... differentiation 1.1.5.2 Pluripotent stem cell- derived NSCs Human embryonic stem cells (hESCs), which are pluripotent cells derived from the inner cell mass of blastocyst-stage embryos, offer a renewable ... of Human Embryonic Stem Cells in mTeSRTM1” (STEMCELL Technologies Inc.) In brief, H1 hESCs were maintained on BD matrigel hESC-qualified matrix (BD Biosciences) with mTeSRTM1 medium (STEMCELL ... HUMAN EMBRYONIC STEM CELL- DERIVED NEURAL STEM CELLS: DERIVATION, DIFFERENTIATION AND MICRORNA REGULATION KWANG WEI XIN TIMOTHY...
- 146
- 511
- 0
Guidelines for Human Embryonic Stem Cell Research potx
Ngày tải lên :
06/03/2014, 15:20
... Guidelines for Human Embryonic Stem Cell Research http://www.nap.edu/catalog/11278.html GUIDELINES FOR HUMAN EMBRYONIC STEM CELL RESEARCH Committee on Guidelines for Human Embryonic Stem Cell Research ... for Human Embryonic Stem Cell Research http://www.nap.edu/catalog/11278.html Scientific Background of Human Embryonic Stem Cell Research INTRODUCTION Human embryonic stem cells (hES cells) are ... this report are common to other areas of human stem cell research, such as research with adult stem cells, fetal stem cells, or embryonic germ cells derived from fetal tissue Institutions and investigators...
- 179
- 330
- 0
Báo cáo y học: "A mouse embryonic stem cell bank for inducible overexpression of human chromosome 21 genes." ppsx
Ngày tải lên :
09/08/2014, 20:22
... spreads were analyzed to assess the percentage of euploid cells Embryonic stem cell differentiation The EB3 cells and the parental line E14 cells [43] were allowed to differentiate using the 'hanging ... HPRT-deficient (Lesch-Nyhan) mouse embryos derived from germline colonization by cultured cells Nature 1987, 326:292-295 Wobus AM, Guan K, Yang HT, Boheler KR: Embryonic stem cells as a model to study ... results obtained from the non-induced state (that is, cells grown in the presence of Tc) with those derived from cells grown in a medium deprived of Tc for 48 hours (in other words, cells overexpressing...
- 18
- 460
- 0
Báo cáo y học: "A comparative analysis of DNA methylation across human embryonic stem cell lines" docx
Ngày tải lên :
09/08/2014, 23:20
... base pair; BS-seq: whole genome bisulfite sequencing; ESC: embryonic stem cells; FDR: false discovery rate; HESC: human embryonic stem cell; HGNC: HUGO Gene Nomenclature Committee; RNA-seq: whole ... island shores distinguishes human induced pluripotent stem cells, embryonic stem cells and fibroblasts Nat Genet 2009, 41:1350-1353 Huang da W, Sherman BT, Lempicki RA: Systematic and integrative ... browser, Amander Clark for providing embryonic stem cell DNA (HSF1) and discussion P-YC is funded by Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research at UCLA SF is Special...
- 12
- 458
- 0
Báo cáo y học: " Human embryonic stem cell (hES) derived dendritic cells are functionally normal and are susceptible to HIV-1 infection" pot
Ngày tải lên :
10/08/2014, 05:20
... Jones JM: Embryonic stem cell lines derived from human blastocysts Science 1998, 282:1145-1147 Reubinoff BE, Pera MF, Fong CY, Trounson A, Bongso A: Embryonic stem cell lines from human blastocysts: ... precursors from human embryonic stem cells Nat Biotechnol 2001, 19:1129-1133 Kaufman DS, Hanson ET, Lewis RL, Auerbach R, Thomson JA: Hematopoietic colony-forming cells derived from human embryonic stem ... Figure Derivation of DCs from hES cells Derivation of DCs from hES cells: Undifferentiated hES cells were cocultured with S17 mouse stromal cells to derive cystic bodies Later, purified CD34+ cells...
- 9
- 261
- 0
Báo cáo y học: " GeneChip analysis of human embryonic stem cell differentiation into hemangioblasts: an in silico dissection of mixed phenotypes" docx
Ngày tải lên :
14/08/2014, 08:20
... derived from human embryonic stem cells Proc Natl Acad Sci USA 2001, 98:10716-10721 Lu S-J, Li F, Vida L, Honig GR: CD34+CD38- hematopoietic precursors derived from human embryonic stem cells exhibit ... hematopoietic differentiation of human embryonic stem cells Blood 2003, 102:906-915 Woll PS, Martin CH, Miller JS, Kaufman DS: Human embryonic stem cell- derived NK cells acquire functional receptors ... development from human embryonic stem cell lines Exp Hematol 2005, 33:987-996 Zambidis ET, Peault B, Park TS, Bunz F, Civin CI: Hematopoietic differentiation of human embryonic stem cells progresses...
- 19
- 359
- 0
Roles of long non coding RNAs in human embryonic stem cell pluripotency and neural differentiation 1
Ngày tải lên :
09/09/2015, 17:54
... protein MEF Mouse embryonic fibroblast xiv Chapter I – Introduction 1.1 Transcriptional control of stem cell pluripotency Embryonic stem cells (ESCs) are derived from the inner cell mass of blastocysts, ... differentiation has been carried out in human cells In this thesis, functional characterization of lncRNAs in human development is addressed using human embryonic stem cells (hESCs) as a paradigm for ... ………………………………………………………… 1.1 Transcriptional control of stem cell pluripotency 1.1.1 The transcription factors OCT4, NANOG and SOX2 constitute the human embryonic stem cell transcriptional core 1.1.2 An expanded...
- 83
- 381
- 0
Roles of long non coding RNAs in human embryonic stem cell pluripotency and neural differentiation 3
Ngày tải lên :
09/09/2015, 17:54
... pluripotency, neural stem cell identity, or neuronal differentiation RNA from the highly homogenous cell types derived from hESCs were hybridized onto two separate arrays: the custom-designed human lncRNA ... that lincRNAs maintain the pluripotent cell state The studies on lncRNAs and pluripotency were all performed in mouse embryonic stem cells (mESCs) Results from the mouse studies may not be directly ... neuronal differentiation respectively 82 Chapter VI – Long Non-coding RNAs Regulate Human Embryonic Stem Cell Pluripotency 6.1 Introduction LncRNAs are integral members of the ESC self-renewal...
- 55
- 327
- 0
Roles of long non coding RNAs in human embryonic stem cell pluripotency and neural differentiation 4
Ngày tải lên :
09/09/2015, 17:54
... confirmed by qPCR in two independent cell lines: hESC (H1)-derived NPCs and an immortalized human neural stem cell line ReN-VM In both hESC-derived cells and ReN-VM cells, RMST expression was upregulated ... AF429305 and AF429306 (Figure 7.1 and Figure 8.4) In humans, RMST is located on chromosome 12q23, and the ENCODE ChIP-seq data from human embryonic stem cells (H1) and neuroblastomas (PFSK-1) indicated ... factor expressed in neural stem cells and non-neuronal cells, to repress neuronal gene expression To confirm that REST indeed binds upstream of RMST in the neural stem cell line ReN-VM, REST chromatin-immunoprecipitation...
- 13
- 275
- 0
Roles of long non coding RNAs in human embryonic stem cell pluripotency and neural differentiation 5
Ngày tải lên :
09/09/2015, 17:55
... E.R (2009) Isolation and characterization of neural crest stem cells derived from in vitro-differentiated human embryonic stem cells Stem Cells Dev 18, 1059-1070 Johnson, R., Teh, C.H., Jia, H., ... of pluripotent stem cells from adult human fibroblasts by defined factors Cell 131, 861-872 Takahashi, K., and Yamanaka, S (2006) Induction of pluripotent stem cells from mouse embryonic and adult ... neural crest stem cells derived from human embryonic stem cells Nat Biotechnol 25, 1468-1475 Lee, S.M., Danielian, P.S., Fritzsch, B., and McMahon, A.P (1997) Evidence that FGF8 signalling from the...
- 34
- 316
- 0
Human embryonic stem cell derivatives as cancer therapeutics
Ngày tải lên :
09/09/2015, 18:52
... factor Human embryonic stem cell Human embryonic stem cell derived dendritic cell Human embryonic stem cell derived mesenchymal stem cell Human embryonic stem cell derived neural stem cell Hematopoietic ... groups reported the derivation of human embryonic stem cells (hESCs) from embryos at the morula stage (30-40 cells) (Strelchenko et al 2004), arrested embryos (arrested at 16-24 cell stage) (Zhang ... dendritic cell Myeloid progenitor cells Mesenchymal stem cell Natural killer T cell Neural stem cell Poly(2-hydroxyethylmethacrylate) Protein phosphatase 1, regulatory subunit 12C Pluripotent stem cell...
- 166
- 294
- 0
n vitro bioassembled human extracellular matrix and its application in human embryonic stem cell cultivation 1
Ngày tải lên :
11/09/2015, 10:06
... fibroblasts derived from fetal skin and muscle of aborted embryos, or from adult tissues such as Fallopian tubes and foreskin [16] However, at present, no GMP-grade xeno-free human feeder cells are available ... process Human feeders such as foreskin fibroblasts also have a limited lifespan, fibroblasts from fallopian tubes are limited in number, and the acquiring of human feeders from aborted embryos ... costeffective culture systems In view of the imperfections of existing culture systems, an optimal system for the culture and derivation of hESCs would require feeder-free culture on a human ECM, maintained...
- 45
- 284
- 0
n vitro bioassembled human extracellular matrix and its application in human embryonic stem cell cultivation 2
Ngày tải lên :
11/09/2015, 10:06
... Isoform of 100.00% 3.33% 47 IPI00220967, IPI00744135, IPI00856098 IPI00009865 58811 IPI00220327 Extracellular Matrix Ribosome-binding protein 66022 IPI00297646 138923 IPI00304962 129297 IPI00013079 ... uncharacterized protein ALB 100.00% 7.81% Table 2: Consolidated list of protein identifications compiled from mass spectrometric analysis of DxSDOC and DxSDOCDOC matrices Mass spectrometry analysis was ... GFR and standard Matrigel, based on work by Hughes et al 2009 † Murine protein is a homolog of human protein 50 ...
- 5
- 219
- 0
n vitro bioassembled human extracellular matrix and its application in human embryonic stem cell cultivation 3
Ngày tải lên :
11/09/2015, 10:06
... system Subculture was done every 5-7 days depending on the confluency of the hESCs In this way, hESCs were maintained for up to 20 passages At each passage, representative cultures of hESCs from ... representative cultures of hESCs from each matrix condition were sacrificed for cell counting using a haemacytometer Population doublings from each passage were calculated and the data plotted for comparison ... adhere to DxSNP40/DNase matrices, and settled on the bottom of the culture well as clusters of cells that were easily moved by mechanical disturbances to the plate (Figure 4B) Likewise, hESCs...
- 7
- 212
- 0
n vitro bioassembled human extracellular matrix and its application in human embryonic stem cell cultivation 4
Ngày tải lên :
11/09/2015, 10:07
... hESCs [9] The pattern of Oct-4 expression in human embryos is similar to that of mouse embryos [10], where Oct-4 is expressed in totipotent embryonic cells, then becomes restricted to the ECM of ... ratios, resembling spontaneously differentiated cells Although hESCs on DxSDOCDOC did have some spontaneously differentiated cells in the population, these cells were not common Colonies of hESCs on ... is a sign of the aging of the cells hESCs on feeder-free culture systems tend to acquire senescence at earlier passages compared to those on feeder co-culture systems, and senescence is usually...
- 16
- 232
- 0
n vitro bioassembled human extracellular matrix and its application in human embryonic stem cell cultivation 5
Ngày tải lên :
11/09/2015, 10:07
... differentiation using a protocol modified from WiCell Research Institute, National Stem Cell Bank In this protocol, the hESCs formed embryoid bodies using non-adherent cell culture plates and were transferred ... regulating cellular differentiation, and because little is known about the epigenetic factors that define the differentiation state or potency of stem cells in comparison to differentiated cells, ... were done The goal was to identify methylation changes between hES cells grown on the human matrices versus Matrigel Samples from this propagation trial were collected and analyzed by Illumina...
- 14
- 209
- 0
n vitro bioassembled human extracellular matrix and its application in human embryonic stem cell cultivation 6
Ngày tải lên :
11/09/2015, 10:07
... Marshall VS Embryonic stem cell lines derived from human blastocysts Science 282:1145–1147 (1998) Reubinoff BE, Pera MF, Fong CY, Trounson A, Bongso A Embryonic stem cell lines from human blastocysts: ... signatures of seven individual uman embryonic stem cell lines Stem Cells 23:1343-1356 (2005) Stewart R, Stojkovic M, Lako M Mechanisms of self-renewal in human embryonic stem cells Eur J Cancer 40:1257-1272 ... made at each human embryonic stem cell culture step that can contribute to a safer and more efficient human embryonic stem cell culture: For the enhancement of collagenous extracellular matrix,...
- 35
- 249
- 0
A human embryonic stem cell based model of trophoblast formation
Ngày tải lên :
11/09/2015, 16:07
... 1.3 Embryonic stem cells 1.3.1 Mouse embryonic stem cells 1.3.1.1 Derivation of mESC mESCs were first derived in 1981 {Martin, 1981 #292; Evans, 1981 #293} mESCs are pluripotent cells derived from ... immune cells 20 1.2.3.5 Other placenta related factors 21 iv 1.2.4 Clinical relevance of placenta development 1.3 Embryonic stem cells 1.3.1 Mouse embryonic stem cells 22 23 23 1.3.1.1 Derivation ... Mouse trophoblast stem cells Mouse trophoblast cells have not been derived from mESC, however, mouse trophoblast stem (TS) cell lines can be derived from the blastocysts Mouse TS cells can be maintained...
- 205
- 261
- 0
Báo cáo khoa học: Polypyrimidine tract-binding protein is essential for early mouse development and embryonic stem cell proliferation potx
Ngày tải lên :
07/03/2014, 00:20
... genes in EBs from Ptb) ⁄ ) ES cells than in EBs from wild-type cells (Fig 4A) The expression of the undifferentiated stem cell marker Nanog is downregulated in both Ptb) ⁄ ) ES cell clones (Fig ... ES cells may be G2 ⁄ M phase delay We next analyzed cell cycle progression in Ptb) ⁄ ) ES cells by arresting the cells in the early S phase with a double thymidine block We released cells from ... vector into Ptb+ ⁄ ) ES cells, which we Table Genotypes of established ES cell lines from blastocysts Fifty-one blastosysts from heterozygous intercrossing were used for ES cell derivation After 2–3...
- 11
- 454
- 0