electrospray ionization and ion trap mass spectrometry it ms

... pretreated with NPDS, and after digestion incubated with propionyl-CoA The identity of domains present in each peak is indicated, together with their inferred acylation status Separation conditions ... studies In our work, ion trap mass spectrometry was used Compared with the Fourier transform -ion cyclotron resonance (FT-ICR) mass spectrometer [16,17], the ion trap is less expensive and more widely ... conclusion, our strategy for limited proteolysis in combination with on-line liquid chromatography ion trap mass spectrometry studies on the multifunctional proteins, DEBS1-TE, DEBS3 and DKS...

Ngày tải lên: 16/03/2014, 18:20

... fragments, fractionate the complex mixture and use high-resolution electrospray ionization Fourier-Transform mass spectrometry Changing patterns of covalent active-site occupancy (ESI ⁄ FTMS) to pick ... for mass deconvolution The detailed conditions for limited proteolysis and LC ⁄ MS analysis have been described previously [37] Substrate specificity for the chain-extender unit A mm concentration ... [12,42] and in vitro [43] 7064 Evidence for coordination of condensation and ketoreduction In previous work [44] the operation of a single extension module from DEBS was studied in vitro by mixing...

Ngày tải lên: 16/03/2014, 03:20

... 100% acetonitrile The solutions were combined and concentrated in a SpeedVac iTRAQ labeling and LC-ESI MS/ MS analysis To label peptides with the iTRAQ reagent (Applied Biosystems, Foster City, CA, ... digestion so that it would not interfere with the LCMS ⁄ MS analysis In addition, we also utilized a recently developed and widely used multiplexed quantitation strategy based on iTRAQ isobaric ... utilized a technology combining gel-assisted digestion, isobaric tags with related and absolute quantitation (iTRAQ) labeling and LC -MS ⁄ MS for quantitative analysis of the membrane proteome of colorectal...

Ngày tải lên: 16/02/2014, 15:20

... ionization' methods such as field desorption and electrospray ionization tend to produce mass spectra with little or no fragment -ion content Gas-Phase Ionization These methods rely upon ionizing ... require either desorption or desolvation methods if they are to be analyzed by mass spectrometry Although ionization and desorption/desolvation are usually separate processes, the term "ionization ... ionization and desorption (or desolvation) methods The choice of ionization method depends on the nature of the sample and the type of information required from the analysis So-called 'soft ionization' ...

Ngày tải lên: 22/03/2014, 14:20

... Determination with Quadrupole MS 6.5.1 Impurity Identification with LC -MS 6.5.2 Impurity Identification with GC -MS 6.5.3 Pros and Cons of Determination of Elemental Decomposition (DEC) with Quadrupole MS ... Compositions by Simple Screening HPLC -MS and Infusion Orbitrap MS 4.9 Determining Substructures: Orbitrap Tandem Mass Spectrometry (MSn) 4.10 Multianalyzer (Hybridized) System: The Linear Ion Trap/ Orbitrap ... electrospray- assisted desorption /ionization electrosonic spray ionization electron transfer dissociation enzyme unit fast-atom bombardment field flow fractionation field-induced droplet ionization...

Ngày tải lên: 28/03/2014, 10:20

... (metabolite candidates) with comparably few dimensions (conditions) In contrast, sample-based clustering implies only few intensity vectors according to the number of conditions and repetitions ... replicas for each condition The identification of metabolites was verified by exact mass measurement and coelution with authentic standards Data processing The raw mass spectrometry data of all ... depends on reproducibility and biological interpretability Without prior selection, large amounts of non-informative markers with little intensity variation across different conditions would dominate...

Ngày tải lên: 12/08/2014, 17:20

... equipped with an electrospray ionization (ESI) source and an ion trap analyzer for UV and MS data acquisition A reverse phase C18 AQ (Alltech, Alltima, 250 × 4.6 mm, μm) column Page of with a 300SB-C18 ... chromatography -electrospray ionization tandem mass spectrometry (HPLC-ESI -MS/ MS) Anal Chim Acta 2006, 567(2):218-228 28 Xie PS, Leung AY: Understanding the traditional aspect of Chinese medicine ... because of nucleosides and nucleobases are basic compounds and are more likely to be ionized with cations such as H+, K+, and Na + , thus facilitating the ESI -MS detection Figure shows Page of...

Ngày tải lên: 13/08/2014, 15:21

... the two soft ionization methods - electrospray ionization (ESI) and matrixassisted laser desorption /ionization (MALDI) (Karas and Hillenkamp, 1988; Fenn et al., 1989; Hillenkamp and Karas, 1990) ... characterization is the utilization of tandem mass spectrometry (MS/ MS) and proteolytic digestion for protein sequence analysis and for the localization of posttranslational modifications Trypsin ... Protein Complex by Tandem Affinity Purification (TAP) Tag Pull Down and Mass Spectrometry 23 3.1 Introduction 3.1.1 Mass Spectrometry (MS) In the post-genome era, mass spectrometry (MS) has emerged...

Ngày tải lên: 04/10/2015, 15:46

... solution into the spray chamber by self-aspiration The spray chamber and plasma torch are made of highpurity quartz and any seals in the sample introduction and drain systems are replaced with ... orifice and a reduction in sensitivity To prevent carbon deposition, the carbon in the sample is Figure Visual optimization of the oxygen level in the plasma decomposed by reaction with oxygen, ... calibrations in IPA Standard addition at 0, 5, 10 and 20 ppt, showing effective removal of 12C2 and 40Ar12C (potential interferences on 24Mg and 52Cr respectively) Conclusions For More Information...

Ngày tải lên: 05/03/2014, 21:20

... Chemical Ionization giao diện phun điện ES Các giọt dung dịch thu nhỏ tăng điện tích Các phân tử dung môi không tích điện bị loại Ion hóa phun điện ESI (Electrospray Ionization) LC -MS • Tách ... ion đường T-Wave thứ hai: cho MS2 TOF: Lựa chọn V mode/ W mode GC -MS Tách GC Xác định MS Có thư viện phổ chuẩn Interface Mass spectrometer acts as a detector for GC LC -MS • Tách LC Xác định MS ... ghép nối LC MS: • giao diện ion hoá hoá học áp suất thường (atmospheric pressure chemical ionisation) • giao diện phun điện ESI (electrospray) APCI ion hoá hoá học áp suất thường APCI • Nitơ dùng...

Ngày tải lên: 24/05/2014, 20:42

... cause dissolution, atomization and ionization of elements;  extraction of the ions from plasma through a differentially pumped vacuum interface with integrated ion optics and separation on the ... determination after calibration with suitable calibration solutions The relationship between signal intensity and mass concentration is usually a linear one over at least five orders of magnitude ... calibration solution B shall be adjusted to that of the digests 6.12 Optimization solution The optimization solution serves for mass calibration and for optimization of the apparatus conditions,...

Ngày tải lên: 03/07/2016, 18:45

... Meng CK, Wong SF & Whitehouse CM (1989) Electrospray ionization for mass spectrometry of large biomolecules Science 246, 6471 52 Loo JA (2000) Electrospray ionization mass spectrometry: a technology ... frequency-to-m z calibration function [82] Mass spectral acquisition and postprocessing were performed using Bruker xmass 5.0.1 6.0.1 software Titration experiments MS titration experiments were ... (2002) Calcium and peptide binding to folded and unfolded conformations of cardiac troponin C Electrospray ionization and Fourier transform ion cyclotron resonance mass spectrometry Eur J Mass Spectrom...

Ngày tải lên: 07/03/2014, 17:20

... chromatography separations and mass spectrometry detection systems, improvement in sensitivity and simultaneous detection of multiple analytes is possible However, the determination of these kinds ... solution This solution was used as working solution for the preparation of the other standard concentrations for calibration and quality control material For the preparation of internal standards ... instrument units The curtain gas was set to psi MRM (multiple reaction monitoring) mode was chosen to perform the MS- MS detection MRM mode allows a simultaneous registration of all MS- MS transitions...

Ngày tải lên: 20/06/2014, 00:20

... deviations In addition to real-time monitoring, tandem mass spectrometry (MSn) helps to identify unknown species, validate proposed intermediates and further understand the reaction mechanisms To demonstrate ... real-time: a one-step Michael addition reaction of phenylethylamine (PEA) and acrylonitrile in ethanol, and a multiple-step acetylation reaction of benzyl alcohol with acetic anhydride catalyzed ... 22: 2993–2998 DOI: 10.1002/rcm Monitoring of organic chemical reactions using EESI -MS Figure Mass spectra of the Michael addition reaction recorded at 20, 60 and 300 min, respectively Inserts:...

Ngày tải lên: 14/02/2014, 08:20

... °CÆmin)1 and to 310 °C at 10 °CÆmin)1 ES -MS Negative -ion ES -MS and CID MS/ MS were carried out on a Micromass (Manchester, UK) Q-Tof mass spectrometer Nitrogen was used as desolvation and nebuliser ... distinction of chain type and blood-group type (such as Lea/x and Leb/y) of underivatized oligosaccharides by negative -ion electrospray mass spectrometry (ES -MS) with collision-induced dissociation ... 3-linked and one 3,6-disubstituted) and three GlcNAc (one 3,4-disubstituted and two 3-linked) The location of the Fuc residue can be deduced from the CID MS/ MS fragmentation In comparison with the...

Ngày tải lên: 19/02/2014, 12:20

... negative ionization mode, and monitored by SRM Mass selection for the Q1 and Q3 analysers was set on unit resolution Nitrogen was used as ion source gas 1, ion source gas 2, curtain gas and collision ... mL with water/methanol (9:1, v/v) The solution was filtered through a 0.22 ␮m nylon membrane prior to LC -MS/ MS analysis 2.3 Instrumentation LC coupled with electrospray ionization tandem mass spectrometry ... collision gas, with flow rates controlled at 65, 60, 25 and psi, respectively Ion electrospray voltage was 5500 V for positive ionization mode and 4500 V for negative ionization mode The ion source...

Ngày tải lên: 25/08/2016, 22:30

... (an onconase variant with a pyroglutamyl residue at position and leucine replacing methionine at position 23) formation as a function of guanidinium chloride concentration and pH The rate of (Pyr1)-ONC ... variant with a pyroglutamyl residue at position and leucine replacing methionine at position 23) Samples were taken at 0, 1, 2, and 24 h and correspond to an experiment carried out at 37 °C and pH ... variant with a pyroglutamyl residue at position and leucine replacing methionine at position 23) and rONC (wild-type recombinant onconase), respectively 1 Ó FEBS 2004 MS characterization of onconase...

Ngày tải lên: 19/02/2014, 12:20

... because iontrap MS was used, the reported experimental data and flux analysis results provide material for comparison between ion- trap and quadrupole MS in the context of flux quantification Finally, ... contain the equations that relate the measured mass isotopomer distribution of the amino acid with the positional isotopomer fractions of network metabolites (e.g erythrose-4-phosphate and phosphoenolpyruvate, ... variation assigned to the rates of pyruvate, glucose and biomass reflects the accuracy of the detection equipment or kit The standard deviation assigned to the net excretion rates of lysine and...

Ngày tải lên: 08/03/2014, 08:20

... Acquisition and data analysis were performed using the explorer 3.5.2 and data explorer 4.9 software from Applied Biosystems NanoLC-linear ion trap (LTQ)-Orbitrap mass spectrometry Tryptic and ... Biosystems) MALDI-TOF-TOF MS and MS ⁄ MS spectra were acquired with a MALDI-TOF ⁄ TOFÔ 4800 mass spectrometer (Applied Biosystems) in the positive and reflector mode An external calibration was ... and msx-3d [12]) with a mass tolerance of p.p.m We therefore used MALDITOF-TOF and ⁄ or nanoLC-Orbitrap tandem MS to further identify peptides from this list Twenty-five mono-linked peptides and...

Ngày tải lên: 15/03/2014, 00:20

... Low -mass sensitivity 68 1% of the...

Ngày tải lên: 22/03/2014, 14:20