electrophoresis serum and urine

... ng standard Gb3 and was able to detect > ng Gb3 per lL serum sample Gb3 in the serum of wildtype mice was below the background of this assay MDR1 inhibition and GSL storage disease ERT and CsA ... the serum Gb3 levels [9] and the effect of a maintenance dosing of CsA on the recovery of serum Gb3 levels after termination of ERT was determined ERT is an effective means of eliminating serum ... 0.028) The recovery of serum Gb3 post ERT was found to be, to some extent, variable and some mice within both the control and treated groups did not recover detectable serum Gb3 by the 9-week...

Ngày tải lên: 19/02/2014, 07:20

... required for handling NLC and NCE A good protocol is always useful to work with these modalities of chromatography and electrophoresis The working protocol for NCL and NCE is shown in Fig 1.1 and a careful ... proteomics, and drug designing and development programs Our search of the literature and experience dictates that chip-based analytical techniques viz nanoliquid chromatography and nanocapillary electrophoresis ... chromatography and abbreviated as NLC The same definition is also true and complete for capillary electrophoresis, which is also possible on chips, and we have termed it nanocapillary electrophoresis...

Ngày tải lên: 04/06/2014, 14:56

... found between serum AMH levels and both follicle count and androgen levels [9] These correlations were successively confirmed [12], and a further relationship between AMH levels and insulin resistance ... Met-anov and Met-ov groups was observed at baseline and after metformin with regards to the serum levels of SHBG, fasting insulin, GIR, HOMA, and AMH before treatment (Table 1) In both Met-anov and ... testosterone (T), androstenedione (A), dehydroepiandrosterone sulfate (DHEAS), and sex-hormone binding globulin (SHBG)], and metabolic evaluation, including evaluation of fasting glucose and insulin...

Ngày tải lên: 20/06/2014, 07:20

... Fernanda Cássio and Cláudia Pascoal 173 Part Statistical and Bioinformatic Analysis of Electrophoresis Data 197 Chapter 12 Statistical Analysis of Gel Electrophoresis Data Kimberly F Sellers and ... were comparatively analyzed by the standard agarose gel electrophoresis and ethidium bromide staining (AGE/EBS) method and polyacrylamide gel electrophoresis and silver staining (PAGE/SS) Those ... Preparation and running of standard agarose gels        The equipment and supplies necessary for conducting agarose gel electrophoresis are relatively simple and include: An electrophoresis...

Ngày tải lên: 28/06/2014, 15:20

... respectively Thirty-six and 54 of the biomarkers have not yet been analyzed in serum and saliva obtained from patients with SS, and neither have 70 of the analytes in serum and 62 biomarkers in ... profiles from serum and saliva A bead-based multiplex sandwich immunofluorescence assay was used to generate multi-analyte profiles (MAPs) from serum and saliva from the 12 nondiabetic NOD and 12 Balb/ ... adhesion molecule (VCAM)-1 and IgA In saliva, C-C chemokine ligand/C chemokine ligand Sa-C-2 (eotaxin and macrophage-derived chemokine [MDC]) and cytokine Sa-C-3 (CD40 and CD40L [IL-18 borderline])...

Ngày tải lên: 09/08/2014, 10:23

... feces DNA/RNA isolation and HIV1 detection work and drafted the manuscript LC, MD and CS performed urine DNA/RNA isolation and HIV-1 detection, the molecular genetic studies and help to draft the ... (HIV-1) by detection of antibody IgGs to pol and gag proteins of HIV-1 and p24 antigen of HIV-1 in urine, saliva, and/ or serum with highly sensitive and specific enzyme immunoassay (immune complex ... RNA/DNA from urine samples 26-83 ml of urine were collected from the study participants and processed within hours The urine samples were centrifuged at 1500 g for 10 minutes at 4°C The urine pellets...

Ngày tải lên: 10/08/2014, 05:21

... aspiration, disseminated candidiasis with candidal sepsis, wound infection, disseminated varicella, atrial fibrillation, acute renal failure and a subhepatic intra-abdominal candidal abscess Discharge ... this article as: Vanderlan et al., Duodenal enteroglucagonoma revealed by differential comparison of serum and tissue glucagon reactivity with Siemens' Double Glucagon Antibody and DakoCytomation's ... hospital His serum potassium remained low despite all replacement efforts Hypoalbuminemia worsened to 1.6 gm/dL Serum glucagon was measured using Siemens' Double Glucagon Antibody and found to...

Ngày tải lên: 11/08/2014, 12:20

... intracystic instillation of minocycline before and Changes2in serum and cyst fluid CA19-9 levelshydrochloride Changes in serum and cyst fluid CA19-9 levels before and after the intracystic instillation ... that serum CA19-9 levels in serum CA19-9 (U/ml) patients with cholelithiasis complicated by cholangitis frequently showed markedly high values, and serum CA19-9 levels were rapidly decreased and ... stroma: expressions of CA 19-9 and carcinoembryonic antigen in serum and cystic fluid J Gastroenterol 1996, 31:732-736 Horsmans Y, Laka A, Gigot JF, Geubel AP: Serum and cystic fluid CA 19-9 determinations...

Ngày tải lên: 11/08/2014, 21:22

... baseline and follow up time points and between serum and synovial fluid values Non parametric Spearman Rank correlations (rs) were performed to determine correlations between synovial fluid and serum ... 0.012), COMP (P = 0.0001) and MMP-3 (P = 0.0001) The biggest differences between synovial fluid and serum were observed for MMP-3 and COMP with fold differences of 738.8 and 21.2 respectively Smaller ... Biomarker concentrations at baseline and follow-up in serum and synovial fluid after acute knee injury Synovial Fluid Levels Biomarker Baseline Mean ±SD Serum Levels Serum vs Synovial Fluid Follow...

Ngày tải lên: 12/08/2014, 15:22

... NCI-H441, and human fetal lung explants H441 cells resemble bronchiolar epithelial Clara cells in phenotype and produce both SP-A and SP-B mRNA and protein [14] The hormonal regulation of SP-A and ... LY294002, rapamycin and PD98059 were reconstituted in dimethyl sulphoxide as mM, 50 mM, 50 µM and 10 mM stock solutions, respectively, and stored at -80°C in aliquots Insulin causes a timeand dose-dependent ... vehicle or insulin (2.5 µg/ml) for an additional 1, 4, and 24 hours The cells were then rinsed and trypsinized, and nuclei from control and treated cells were harvested The transcription elongation...

Ngày tải lên: 12/08/2014, 18:20

... their serum (P < 0.01 to P < 0.0001) (Table 5) Predictive serum and BALF levels of all studied molecules for surviving are shown in Tables and In contrast to BALF values, serum levels of IL-1 and ... values and the range in BALF and serum of the studied mediators in survivors and non-survivors in all categories are shown in Table All studied mediators were found at higher levels in the serum ... predictive value, sensitivity and specificity for surviving in all groups In a multivariate analysis model adjusting for age and severity we found that serum IL-1 (P = 0,002) and serum ICAM-1 (P = 0,009)...

Ngày tải lên: 12/08/2014, 18:20

... clinically and with laboratory tests every two months regardless of disease activity, and provided blood, a 24 hour urine specimen, and a freshly voided urine specimen at the visit Renal and nonrenal ... patients enrolled in the Ohio SLE Study (I) Clinical measurements of urine MCP-1, urine P:C, serum C3 and serum C4 taken every months, and accompanying therapy (Prednisone (Pred) = corticosteroids, ... patients enrolled in the Ohio SLE Study (II) Clinical measurements of urine MCP-1, urine P:C, serum C3 and serum C4 taken every months, and accompanying therapy (Prednisone (Pred) = corticosteroids,...

Ngày tải lên: 13/08/2014, 16:20

... MODS and MOF patients The time points for the comparisons were on day and for blister fluid samples and days 1, 4, 6, and 10 for serum samples The serum and blister fluid MMP-levels of MODS and ... detected in the serum samples in patients and controls and the 82 kDa MMP-9 could be detected only in few samples (3 on day 1; on day 4; on days 6, and 10; and at and months) At three and six months ... MMP-8 Statistical analysis Serum and blister fluid levels of MMP-8, MMP-9 (92 kDa and 82 kDa forms), and MMP-2 (72 kDa and 62 kDa forms) were compared between non-surviving and surviving patients...

Ngày tải lên: 13/08/2014, 20:21

... ligands in separation techniques such as chromatography, microfluidics and capillary electrophoresis These aptamers can help facilitate the improved purification and separation of proteins and ... bead and purified using PAGE DNA libraries consist of oligonucleotides with a constant region of about 20 bp at both the 5’ and 3’ ends and a 20-50 bp randomized region in the middle Shorter random ... mixture of aptamer and target protein is allowed to reach equilibrium A sample of DNA and the target is allowed to reach equilibrium and is injected The complex, the ligand and target can be separated...

Ngày tải lên: 10/09/2015, 09:22

... presence and persistence of IgG and IgE anti-influenza antibodies in the serum from those with normal and ele- 243 vated levels of IgE are understandable Our findings appear to be of importance and ... RESULTS Characteristics of Study Subjects Serum IgE levels and IgE and IgG anti-Influenza virus antibodies were studied in children (N=3) (m/f 14-16 y/o) and adults (N=3) (m/f, 41-49 y/o) approaching ... Total serum IgE levels were both normal and elevated in adults and children vaccinated with Influenza virus Children with no history of either Influenza virus infection or vaccination had serum...

Ngày tải lên: 25/10/2012, 11:10

... Sample pretreatments and proteomic analysis in the proteomic profiling analysis, the serum samples from the diseased and control groups were randomized, and blinded to investigators Serum samples were ... of Serum Samples in Patients with CRC and Healthy Controls The protein profile of the serum samples from the 98 patients with CRC and the 80 healthy controls were extracted by magnetic beads and ... and had the informed consent of the patients and volunteers The patients and serum samples were then divided into two groups: the ‘‘training’’ set and the blinded ‘‘test’’ set (Table 2).The blood...

Ngày tải lên: 25/10/2012, 11:18

... range of patients and controls were 44-85 year and 43-86 year respectively Table shows serum Na+, K+ of case and control groups, with a significant difference in serum + Na of case and control ones ... senile cataract (79 female, 76 male) and the control one consisted of 155 of cases’ close relatives (79 female, 76 male) Mean age and standard deviation of patients and control group were 61.96±14.5, ... control group and sent to the laboratory Serum Na+, K+ levels were measured with flam photometry method by the aid of SEAC model FLP20, Italy Outcoming data was assessed by spss 11 software and mean...

Ngày tải lên: 03/11/2012, 09:49

... feces and urine composition of the human and animal in Vietnam (Table 3) as well as average rate of excretion (Table 4) Table 3: Chemical composition of excreta and urine of the human and animal ... - Wind Urine collection Solar energy Glass lid Urine fertilizer Fields Urine storage and treatment FOOD Eating Figure 4: Conceptual idea on nutrient cycle in urine collection, treatment and reuse ... phosphorous and potassium (Simon, 2002) Thus, if one can separate the urine and find a suitable to have a mineral fertilizer and then distribute this fertilizer source back to the cultivated lands,...

Ngày tải lên: 16/01/2014, 17:20

... albumin, serum blood urea nitrogen (BUN), and serum creatinine levels (Table 1) Comparison of the serum concentrations of 22 cytokines, chemokines, and growth factors demonstrated that sCD40L and ... increases in the serum concentrations of sCD40L and TGF-a and significant decreases in the serum concentrations of G-CSF, GM-CSF, and MCP-1 Future studies will focus on understanding the significance ... interpretation and in the writing HOK and HSK performed the analysis of the data and drafted the manuscript JCY and ECS participated in the design of the study and performed the statistical analysis and...

Ngày tải lên: 14/02/2014, 06:20

... presence and absence of 0.1 lM angiotensin II and IL-10 BrdU incorporation into cardiac fibroblast under normal 10% fetal bovine serum or serum- free culture conditions IL-10 also decreased type I and ... NF-jB-dependent IL-1b and TNF-a transcription Because transcription of IL-1b and TNF-a can be augmented in MSCs by hypoxia [5], and because the molecular masss of IL-1b and TNF-a are both 17 ... III and a-smooth muscle actin (a-SMA) in cardiac fibroblasts in the presence and absence of IL-10 *P < 0.05 and **P < 0.01 versus Cont group (D) Representative western blots for collagen I and...

Ngày tải lên: 18/02/2014, 04:20