effect of feg on neutrophil chemotaxis adhesion and function
Báo cáo y học: "Tetra-O-methyl nordihydroguaiaretic acid (Terameprocol) inhibits the NF-B-dependent transcription of TNF-a and MCP-1/CCL2 genes by preventing RelA from binding its cognate sites on DNA" ppsx
... reveal an effect of TMP on the nuclear translocation of RelA • Western blots failed to reveal an effect of TMP on the phosphorylation of IB-a • EMSA assays failed to reveal an effect of TMP on the ... transcription, was examined by testing the effects of TMP on TLR-3 and TLR-8-mediated activation of NF-B [26,27] The natural ligands for TLR-3 and TLR-8 are double and single stranded RNA, respectively ... in the transcriptional activation of cytokine genes after LPS stimulation [25] Therefore, we hypothesized, that the inhibitory effects of TMP on the transcription of TNF-a and MCP-1/CCL2 mRNAs...
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Tài liệu Báo cáo khoa học: Solution NMR structure of five representative glycosylated polyene macrolide antibiotics with a sterol-dependent antifungal activity doc
... single conformer under the NMR restraint The lactone function with the conjugated C2–C3 double bond, the C4–C5 epoxide function, the chair conformation of the C9–C13 heterocycle and the conjugated ... at 2.8, 3.3 ˚ and 4.0 A for strong, medium and weak correlations, respectively For candidin, each of the ROE correlations were considered as weak correlations as no information concerning the ... experimental ROE ˚ function calculated using a force constant of 25 kcalÆmol)1ÆA)2 in the case of the CHARMM22 force field, and Fcdih is the experimental function corresponding to the violation of the dihedral...
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Tài liệu Báo cáo Y học: Ornithine decarboxylase-antizyme is rapidly degraded through a mechanism that requires functional ubiquitin-dependent proteolytic activity pot
... translation mix demonstrated that the second initiation codon is utilized preferentially [11,12] Utilization of the two initiation codons with clear preference towards the second one was also inferred ... extracts were prepared and analysed as described in A The molecular weight and position of the two forms of antizyme is indicated on the right *NS indicates the position of nonspecific proteins recognized ... Characterization of antizyme degradation (Eur J Biochem 269) 1317 participate in subsequent cycles of ODC degradation [2,13,21] In contrast, other studies demonstrated rapid degradation of antizyme...
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Báo cáo khoa học: Characterization of the bga1-encoded glycoside hydrolase family 35 b-galactosidase of Hypocrea jecorina with galacto-b-D-galactanase activity pdf
... (4-O-b-d-galactopyranosyl-d-gluconic acid) and methyl-b-d-galactoside was poor The effect of hydrolysis products (each at 10 mm final concentration) on the hydrolysis of oNPG by Bga1 under standard assay conditions was ... N-glycosylation, and thus none of the sites mentioned above could be falsified Optimal temperature and pH for catalysis The optimal temperature and pH for the reaction of Bga1 with oNPG as substrate ... significant part of the total secreted protein Concentration by ultrafiltration and subsequent purification by gel filtration and cation-exchange chromatography (Table 1) yielded a single protein band at...
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Báo cáo khoa học: Lending a helping hand, screening chemical libraries for compounds that enhance b-hexosaminidase A activity in GM2 gangliosidosis cells pptx
... are mapped onto the 3D structure of Hex A (2GK1), shown as a ribbon diagram NGT- and PYR-responsive (green) and nonresponsive (red) mutations in the a- (pink) and b- (blue) subunits of Hex A are ... existence of specific sites on Hex that not block function, and which could be used to stabilize the enzyme against thermal denaturation, is supported by the observation that nonfunction-blocking ... in the concentrations between the a- and b-monomers have the beneficial effect to cells of encouraging heterodimer, Hex A, formation Disease causing missense and partial splice-site mutations can...
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Báo cáo khoa học: Selection of peptides inhibiting a b-lactamase-like activity docx
... conditions of selection Concentrations of rat anti-(mouse IgG), IgG 9G4H9 and phages from the library CX7C are indicated from the first to the sixth round of positive selection on IgG 9G4H9 and ... the first and the second round of negative selection in our experimental conditions On the one hand, there is an increase in the yield of specific peptides recovered, but on the other hand the affinity ... Phage ELISA on clones isolated from the fifth round of positive selection The name of the clone (Fx) corresponds to the family reported on Table to which belongs the clone Open bar corresponds to...
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Báo cáo y học: "Increased interleukin-17 production via a phosphoinositide 3-kinase/Akt and nuclear factor κB-dependent pathway in patients with rheumatoid arthritis" ppt
... rapamycin and PD98059 had no effect on IL17 levels (Fig 3) To evaluate the possibility of non-specific inhibition by the drug at high concentrations, we observed the dose response of PDTC and LY294002 ... stimulates the production of IL-6 and leukemia inhibitory factor by synoviocytes, and of prostaglandin E2 and nitric oxide by chondrocytes, and has the ability to differentiate and activate the dendritic ... calibration standard, ranging from 10 to 2000 pg/ml A standard curve was drawn by plotting optical density against the log of the concentration of recombinant cytokines, and used for determination of...
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Báo cáo y học: "Tumor necrosis factor-alpha promotes survival in methotrexate-exposed macrophages by an NF-B-dependent pathwa" pptx
... reduced availability of purines and pyrimidines for DNA and RNA synthesis and accumulation of AICAR AICAR, by inhibiting enzymatic deamination of adenosine and adenosine monophosphate, is proposed ... physiological conditions Serum concentrations of M-CSF are elevated to approximately 0.6 ng/ml in patients with RA [22], but concentrations in synovium are unknown In vitro, a concentration of 30 ng/ml ... erosions may also protect through NF-B induction Receptor Activator of NF-B Ligand (RANKL) circulates at elevated concentration in RA and is demonstrable in rheumatoid synovium and erosions...
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Tài liệu Báo cáo khoa học: Functional characterization of an orphan cupin protein from Burkholderia xenovorans reveals a mononuclear nonheme Fe2+-dependent oxygenase that cleaves b-diketones ppt
... identical reaction conditions The activity of Fe2+ Bxe_A2876 with b-diketones was characterized by comparing measurements of consumption of O2 and substrate with the formation of detectable products ... O2-dependent conversion of 2,4-pentanedione is shown in Scheme The results are consistent with participation of Fe2+ as a redox-active cofactor in enzymatic catalysis of oxidative carbon– carbon bond cleavage ... electron-deficient carbonyl carbon of the b-diketone moiety is consistent with a nucleophilic mechanism of carbon–carbon bond fission, where the C-3 peroxo intermediate undergoes decomposition via...
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Tài liệu Báo cáo khoa học: Regulation of cathepsin B activity by 2A2 monoclonal antibody docx
... increased concentration of its Fab fragment resulted in a decreased concentration of the cathepsin B ⁄ cystatin C complex and an increased concentration of complexes formed between the Fab fragment and ... presence of various concentrations of 2A2 mAb The binding of 2A2 mAb was followed by native gel electrophoresis Increasing the concentration of 2A2 mAb at a fixed molar ratio of cathepsin B and cystatin ... degradation of DQ-collagen IV and BODIPY FL casein In experiments studying the effect of 2A2 mAb on the stability of the cathepsin B ⁄ cystatin C complex, we demonstrated that increasing concentrations...
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Tài liệu Báo cáo khoa học: Dual P2Y12 receptor signaling in thrombin-stimulated platelets – involvement of phosphoinositide 3-kinase b but not c isoform in Ca2+ mobilization and procoagulant activity pdf
... or in combination with ADP (20 lM) (A) Effect of AR-C (10 lM) preincubation on Ca2+ response (B) Effect of general PI3-K inhibitor LY294002 (LY, 25 lM) on Ca2+ response (C) Effect of PI3-Kb inhibitor ... novel function for the b-isoform of PI3-K Previously, this isoform had been linked to shear-dependent activation of platelets, regulating the stability of platelet adhesion and aggregation [20,21] ... stimulation P2Y12 appears to increase InsP3 receptor function via PKA inhibition This effect is further enlarged by a PI3-Kb-dependent prolongation of phospholipase C activation and InsP3 production...
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Tài liệu Báo cáo khoa học: Glycation of low-density lipoprotein results in the time-dependent accumulation of cholesteryl esters and apolipoprotein B-100 protein in primary human monocyte-derived macrophages docx
... concentrations of these materials present in the artery wall, and in atherosclerotic lesions, are unknown The role of glycation and the two facets of glycoxidation in generating modified LDLs and ... oxidation, aggregation, chemical modification and formation of immune complexes [4,6] Elevated glucose levels are strongly linked to the incidence and severity of atherosclerosis [7,8] Of particular ... under these conditions, due to the lower initial accumulation of nondegraded intracellular apoB (data not shown) Investigation of the nature of the receptors responsible for uptake of glycated...
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Tài liệu Báo cáo khoa học: Design, structure and biological activity of b-turn peptides of CD2 protein for inhibition of T-cell adhesion ppt
... on ROE violation > 0.2 A and allowed values of /, w in the Ramachandran map, only one family of structure that was consistent with NMR data was chosen to represent the conformation of peptide cER ... and the proximity of amide protons due to compact nature of the structure However, the coupling constant of most of the amide protons was in the range of 6–8 Hz, suggestive of rapidly interconverting ... CD2–CD58 interaction Thus, in terms of inhibition of cell adhesion, cVY has more potential since it may directly interrupt the binding mechanism of CD2–CD58 Inhibition of cell adhesion involves interrupting...
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Tài liệu Báo cáo khoa học: The Alzheimer b-peptide shows temperature-dependent transitions between left-handed 31-helix, b-strand and random coil secondary structures doc
... fraction of residues in PII conformation is given by the power of s in the partition function divided by the total number of residues The fraction of PII is then xPII ẳ (N-1))1 d(lnQ) d(lns) and ... radius dependence on number of residues for a polypeptide chain in ideal random coil, b-strand and PII-helix conformations Measurements of translational diffusion for the Ab(1228) and Ab(19) peptides ... irreversible aggregation concomitant with the conversion into b-structure, the favoured secondary structure of the aggregates The central segment 1824, which has a large contribution of b-strand already...
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Tài liệu Báo cáo khoa học: Molecular characterization and allergenic activity of Lyc e 2 (b-fructofuranosidase), a glycosylated allergen of tomato pdf
... of 50 kDa would suggest that we had purified the truncated isoform Cloning of the cDNA of two isoforms of tomato b-fructofuranosidase and recombinant expression in E coli For protein expression ... several modifications Peripheral blood was drawn from nonalllergic donors and PBMCs were isolated using Ficoll-Hypaque centrifugation The conditions for stripping of the nonspecific IgE and for the ... fractions 34–37 contained a band of 36 kDa and the fractions 38–41 a protein with a molecular mass of about 20 kDa (Fig 2B) Immunoblot analysis with a polyclonal anti-profilin serum from rabbit confirmed...
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Tài liệu Báo cáo Y học: Characterization and regulation of yeast Ca2+-dependent phosphatidylethanolamine-phospholipase D activity docx
... pattern and extent of stimulation of the membrane and soluble yeast PtdEtnPLD activity by Ca2+ and their inhibition by other divalent cations is highly comparable The mechanism of action of Ca2+ ions ... an initial activation phase at concentrations of 10)6 to 10)5 M and a Membrane-bound None Ca2+ Mg2+ Co2+ Ba2+ Mn2+ Zn2+ Fig Effect of increasing Ca2+ concentration on membrane and cytosolic PtdEtn-PLD ... Triton X-100 was added The final concentration of Triton X-100 in assay reactions containing C6-NBD-PtdEtn was 0.25 mM The Ó FEBS 2002 hydrolysis of C6-NBD-PtdEtn was monitored by the production of...
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Báo cáo khoa học: The N-terminal hybrid binding domain of RNase HI from Thermotoga maritima is important for substrate binding and Mg2+-dependent activity pot
... domain of reverse transcriptase Retroviral RNases H are required for the conversion of single-stranded genomic RNA into double-stranded DNA, which is an initial step of viral proliferation, and ... domain of Tma-RNase HI The far- and near-UV CD spectra of Tma-ND were significantly different from those of Tma-RNase HI, probably because the secondary structure contents and environment of the ... binding affinity of Tma-RNase HI is reduced by five- to seven-fold on removal of the HBD, regardless of the metal cofactor, and the large reduction in Mg2+-dependent activity on removal of the HBD is...
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Báo cáo khoa học: Autocatalytic processing of procathepsin B is triggered by proenzyme activity doc
... pmol of each of the three oligonucleotides (the two outer and a mutagenic one), 20 nmol of each of the four deoxynucleoside triphosphates, Taq DNA ligase buffer, U of Vent DNA polymerase and U of ... processing by addition of E-64, a broad spectrum inhibitor of cysteine proteases The inhibitor concentrations were varied over a range that was 5–20% of the molar concentration of procathepsin B ... activation of cysteine cathepsins, were shown to induce a conformational change in procathepsin B upon binding, resulting in propeptide removal from the active site cleft and conversion of the...
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Báo cáo khoa học: Analysis of the NADH-dependent retinaldehyde reductase activity of amphioxus retinol dehydrogenase enzymes enhances our understanding of the evolution of the retinol dehydrogenase family pot
... responsible for the in vivo oxidation of retinol remains uncertain In previous studies, we analyzed the functionality and evolution of the MDR-ADH family [5–8,9] Here, we focus on the contribution ... centrifugations (lanes and 3, microsomal fractions) of homogenates of COS cells transfected with HA-RDH1 (lanes and 2) and HA-RDH2 (lanes and 4) (E–L) In vivo localization of RDH2 in the ER of the ... enzymes, and generated 64 and 134 pmol of retinol in the same assay conditions Overall, transfection with RDH1 and RDH2 cDNA increased the level of retinaldehyde reduction by 35% and 50%,...
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Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản xác định thời lượng học về mặt lí thuyết và thực tế tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra đối với đối tượng giảng viên và đối tượng quản lí điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct mở máy động cơ lồng sóc hệ số công suất cosp fi p2 đặc tuyến hiệu suất h fi p2 đặc tuyến dòng điện stato i1 fi p2 động cơ điện không đồng bộ một pha sự cần thiết phải đầu tư xây dựng nhà máy phần 3 giới thiệu nguyên liệu từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008 chỉ tiêu chất lượng 9 tr 25