efficient method of moments

... deprived of the 2¢-OH group of ribose. We showed the silencing effect of targeted RNA in HeLa cells to be at the level of approximately 78% when 100 nm siDNA was used (Fig. 2). Applica- tion of higher ... slightly less efficient than in the case of dsRNA, and a higher dose of siDNA is required for efficient silencing, dsDNA shows higher cellular stability than dsRNA. The other advantages of AB Fig. ... phases of RNAi. Ago proteins are key components of RISC responsible for the cleav- age of the target RNA. The cleavage is catalysed by a Piwi domain of Ago proteins, the structural homolog of RNase...

Ngày tải lên: 16/03/2014, 02:20

... pY i,1  −L −1 i  ∞  n0 a i,n P n  t   . 3.10 Equating the coefficients of like powers of p, we get the following set of equations: coefficient of p 0 : Y 0  L −1  ∞  n0 a i,n P n  t   , coefficient of p 1 : Y 1  L −1 i  f i   ... corresponding solutions of the integer-order equations are found to follow as special cases of those of fractional-order equations. We consider the system of fractional-order equations of the form D α i y i  t   ... Article On Efficient Method for System of Fractional Differential Equations Najeeb Alam Khan, 1 Muhammad Jamil, 2, 3 Asmat Ara, 1 and Nasir-Uddin Khan 1 1 Department of Mathematics, University of Karachi,...

Ngày tải lên: 21/06/2014, 05:20

... empirical implications of SV models on option pricing have not yet been adequately tested. The purpose of this paper is to first estimate a multivariate SV model using the efficient method of moments (EMM) ... and the Efficient Method of Moments (EMM) by Gallant and Tauchen (1996). While the stochastic volatility generalization has been shown to improve over the Black-Scholes model in terms of the explanatory ... biases of the model option prices, while the MARE statistic measures the dispersion of relative biases of the model prices. The difference between MARE and MRE suggests the direction of the bias of...

Ngày tải lên: 21/12/2013, 01:20

... potentia- tion of the pro-apoptotic effect of doxorubicin was found, whereas inhibition of STAT3 with a dominant negative or a platinum derivative increased the pro-apoptotic effect of doxorubicin ... doxorubicin [43]. Thus, the efficiency of blocking STAT3 may depend on the absence of the inhibition of STAT1. Indeed, cell death induced by ODN and by IFN-c may be the result of completely different mechanisms. ... increased by more than twofold (Fig. 6B). In the absence of IFN-c treatment, there was a low but clearly detectable phosphorylation of STAT3. Finally, to analyse the interaction of both STAT1 and STAT3...

Ngày tải lên: 18/02/2014, 08:20

... ribozymes as efficient molecular tools for the inhibition of the biosynthesis of b-secretase and for the exclusion of the formation of toxic b-amyloid peptides in HEK293 cells. Materials and methods Construction ... of the BACE mRNA level because of the lower expression of ribozyme. A similar profile of the target mRNA was obtained for the cells transfected with the Rz-2 plasmid (data not shown). Effect of ... level of extra- and intracellular release of b-amyloid peptide, which is a product of the catalytic activity of the target protein. Our efforts to use Western blot analysis for detection of the...

Ngày tải lên: 08/03/2014, 08:20

... start of reaction. With the use of this low-DTT extract, the scFvLH mRNA was translated in the presence of [ 14 C]Leu, and the productivity was monitored by measuring the radioactivity of the ... detection of antigens were also possible with the use of this immobi- lized surface. Keywords: cell-free synthesis; scFv; disulfide; wheat germ; biotinylation. As a consequence of the successes of the ... quantities of cDNA-encoded proteins and highly parallel synthesis of many different proteins under unified reaction conditions [1,2]. The expression of a cDNA clone in this cell-free method can...

Ngày tải lên: 16/03/2014, 23:20

... (sec) Log−likelihood Sparse Method 1 Method 2 Method 3 Method 4 (a) PTB 0 500 1500 2500 −10000 −6000 Training time (sec) Log−likelihood Dense Sparse Method 1 Method 2 Method 3 Method 4 (b) CoNLL00 0 ... (sec) Log−likelihood Dense Sparse Method 1 Method 2 Method 3 Method 4 (c) CoNLL03 0 1000 3000 5000 −41000 −39000 Training time (sec) Log−likelihood Dense Sparse Method 1 Method 2 Method 3 Method 4 (d) NetTalk 0 ... (sec) Log−likelihood Dense Sparse Method 1 Method 2 Method 3 Method 4 (e) Communicator 0 100000 200000 300000 −30000 −20000 −10000 Training time (sec) Log−likelihood Sparse Method 1 Method 2 Method 3 Method 4 (f)...

Ngày tải lên: 23/03/2014, 17:20

... equation (3), we see that the number of MDP states will grow exponentially with the length of the dialogue, proportionately to the num- ber of ASR-SLU hypotheses. Some of the state- hypothesis pairs ... Management James Henderson University of Geneva Department of Computer Science James.Henderson@cui.unige.ch Oliver Lemon University of Edinburgh School of Informatics olemon@inf.ed.ac.uk Abstract In ... with the MDP system of (Lemon and Liu, 2007), 3 tasks with a state -of- the-art hand- coded system (see (Lemon et al., 2006)), and 3 tasks with the MM Q-MDP system. Ordering of sys- tems and tasks...

Ngày tải lên: 31/03/2014, 00:20

... acti- vation of cellular signalling. Development of an efficient modelling and simulation of the response of signalling protein phosphorylation o n multiple, complex combina- tions of stimuli and ... cover all combinations of stimuli and inhibitors from k = 1, ,m, then all coefficients of F up to interactions of order m can be estimated from the data. In each experiment coefficients will be ‘active’ ... in detail: • Effect(s) of inhibition on the topology of a co-regulatory network • Localization of the apparent inhibition of a signalling protein upstream or down- stream of crosslinks between...

Ngày tải lên: 21/06/2014, 02:20

... Efficient Method for Proportional Differentiated Admission Control Implementation Vladimir Shakhov 1 and Hyunseung Choo 2 1 Institute of Computational Mathematics and Mathematical Geophysics of ... previous solutions of the problem and offer an efficient nonasymptotic method for implementation of proportional differentiated admission control. 1. Introduction Efficient implementation of admission control ... results of numerous experiments with the proposed method. Section 5 is a brief conclusion. 2. Problem Statement Let us consider the concept of admission control inspired in the framework of proportional...

Ngày tải lên: 21/06/2014, 05:20

... typically results in a sequence of length N a + N b −1. 2. System Model Let S be an uncoded frequency domain OFDM symbol of size (N × 1) where N is the number of tones. This symbol is transformed ... parameter  Q rf [l]. A 2-tap FEQ is then employed for the estimation of the transmitted OFDM symbol  S[l]. (ii) In the case of predistortion of transmitted symbols (Section 3.2), we can track the variation ... compensation of channel distortion in an orthogonal frequency division multiplexing (OFDM) systems. The presence of frequency selective channel fading is a requirement for the estimation of IQ imbalance...

Ngày tải lên: 21/06/2014, 08:20

... pitch of 177 Hz is positioned at a frequency that is nearly half that of the true pitch of 353 Hz. 3.2.4. Removal of E xtra Pitches by Checking Harmonic Com- ponents. By means of a large number of ... involve the process of estimating the polyphonic number of the analyzed sound. So as to solve the problem of coinciding frequency partials, the basic idea of the new proposed method is completely ... Other State -of- the-Art Methods. In order to compare our technique with other state -of- the-art approaches, the new method was submitted to the multiple fundamental frequency frame level estimation task of...

Ngày tải lên: 21/06/2014, 19:20

... bank method, we can obtain the speed advantage of the parallel read with the nonreplicating storage required by the sequential reading method. The advantages of using this multiple bank method ... from contents of Q1_C Read from contents of Q2_WNES Ignore contents of Q1_WNES Write to Q2_WNES Write to Q1_WNES Read from contents of Q1_WNES Write to Q2_WNES Figure 20: An example of how Pixel ... will have been written to Q1. To keep track of the number of elements in Q1 WNES, two sets of memory counters are used. These two sets of counters consist of mc1 → mc4inonesetandmc6 → mc9 in another....

Ngày tải lên: 21/06/2014, 20:20

... t r ). Proof. For the scenarios considered here, if the deadline of the message at the head of each trip can be met, the deadlines of all other messages can also be met. Denote the delay of the ... message at the head of (k +1) th trip as D k+1 .AscanbeseenfromFigure 2, the starting point of pickup for k th trip is between the time points of the arrival of the tail message of k th trip and ... important application of pigeon networks—a special type of delay tolerant networks (DTNs) that borrows the ancient idea of using pigeons as the telecommunication method. The aim of this paper is to...

Ngày tải lên: 21/06/2014, 23:20

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Ngày tải lên: 22/06/2014, 22:20