eem fluorescence spectroscopy and synchronous fluorescence sf spectroscopy
Interactions between early germ cells and inner germarial sheath cells in drosphila ovary
Ngày tải lên :
14/09/2015, 08:42
... image of five EGFR ligands in Drosophila and intracellular cleavage and trafficking of Spi Panel A shows activatory and inhibitory ligands (precursors) of EGFR Gurken, Keren, and Spitz are produced ... (female sterile (1) Yb) and Hh (King and Lin, 1999; King et al., 2001), and pathways including Dpp signaling (Xie and Spradling, 1998), JAK-STAT (Janus kinase-Signal Transducers and Activators of Transcription) ... including GSCs and CBs, to cleave multiple EGFR ligand precursors to form active ligands; then the active ligands bind to EGFR expressed in IGS cells to activate EGFR/MAPK signaling; and the EGFR/MAPK...
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Tài liệu Báo cáo khoa học: Bone morphogenetic protein signaling in stem cells ) one signal, many consequences pptx
Ngày tải lên :
19/02/2014, 00:20
... activate R-Smads (Smads 1, and for BMP ligands) by serine-threonine phosphorylation [1,2] R-Smads are transcription factors, which need activation (usually by phosphorylation) and subsequent multimerization ... ligand-binding and resulting differences in downstream targets have also been clarified in much more detail [1] Regulation is further fine-tuned by BMP receptor regulation through degradation and ... Among the hot topics in current research is the generation and use of stem cells The theoretical applications of having expandable and differentiation-controllable stem cell cultures are extremely...
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Báo cáo y học: "Global analysis of alternative splicing regulation by insulin and wingless signaling in Drosophila cells" ppt
Ngày tải lên :
14/08/2014, 21:20
... categories and and the regulated Acknowledgements We thank Konrad Basler, George Hausmann and Thomas Radimerski for material and suggestion on signaling pathway activation, George Pyrowolakis and Roderic ... into cDNA and labeled with Cy5 or Cy3 fluorochromes; after hybridization of the cDNA to the microarray, the ratio of fluorescence between the Cy5 and Cy3 signals was measured, normalized and a Z-score ... cell growth and differentiation Materials and methods Cell culture assays, western blotting and RNA isolation For insulin pathway activation, S2 cells were grown to exponential phase and 30 g/ml...
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Báo cáo khóa học: ICAM-1 expression is highly NF-jB-dependent in A549 cells No role for ERK and p38 MAPK docx
Ngày tải lên :
07/03/2014, 15:20
... inhibitors, PD098059 and U0126, were validated by analysis of phosphorylation of ERKs and 2, which lie downstream of MEK1 [24] Both TNFa and IL-1b upregulated phosphorylation of p42/44 ERKs and preincubation ... only implicates the a and b isofroms of p38, as SB203580 has little or no effect on p38 c and d and it is possible that this could explain discrepancies between over-expression and inhibitor based ... ICAM-1 expression (Eur J Biochem 271) 787 (both phospho and pan ERK 1/2) (New England BioLabs, Hertfordshire, UK) at a dilution of : 1000 and incubated overnight at °C In all cases, proteins were...
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Báo cáo khoa học: Mammalian 105 kDa heat shock family proteins suppress hydrogen peroxide-induced apoptosis through a p38 MAPK-dependent mitochondrial pathway in HeLa cells potx
Ngày tải lên :
16/03/2014, 06:20
... shock and anticancer drugs [17] In addition, Hsp105a suppresses protein aggregation and apoptosis caused by the expression of proteins with expanded polyQ tracts in a cellular model of spinal and ... anti-G3PDH Hsp60 and G3PDH are marker proteins for the mitochondrial and cytoplasmic fractions, respectively The intensity of bands was quantified by public-domain NIH IMAGE software, and normalized ... proteins Bcl-2 and Bcl-XL and inhibits their prosurvival function [36–39] In addition to Bcl-2 and Bcl-XL, previous studies have also implicated the proapoptotic BH3-only proteins Bid and Bim in...
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Báo cáo khoa học: Intrabodies against the EVH1 domain of Wiskott–Aldrich syndrome protein inhibit T cell receptor signaling in transgenic mice T cells potx
Ngày tải lên :
16/03/2014, 14:20
... between scFvs and WASP The scFv 21SHL and 21SHL-CL transgenic T and B cell lysates were immunoprecipitated with WASP mAb and Myc tag mAb and analyzed by Western blotting with Myc tag mAb and WASP ... scFvs and in vivo interaction between scFvs and WASP in scFv transgenic mice T and B cells (A) Western blot analysis of protein extracts of T and B cells from the spleens of the 21SHL and 21SHL-CL ... Immunoprecipitates with Myc tag antibody (lanes and 2) and cell lysates (lanes and 4) from anti-WASP scFv 21SHL transgenic (lanes and 3) or 21SHL-CL transgenic (lanes and 4) mice T cells were analyzed by...
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Báo cáo khoa học: Liver receptor homolog-1 localization in the nuclear body is regulated by sumoylation and cAMP signaling in rat granulosa cells docx
Ngày tải lên :
23/03/2014, 06:20
... adipose and may play a role in breast tumor development [13,14] Nuclear hormone receptors are typically ligand-activated transcription factors and their activities are modulated by coactivators and ... Interactions between prostaglandin E(2), liver receptor homologue-1, and aromatase in breast cancer Cancer Res 65, 657–663 Aranda A & Pascual A (2001) Nuclear hormone receptors and gene expression Physiol ... in stability [22] and transcriptional activity [23,24] SUMO conjugation is dynamic and reversible SUMO-specific proteases (SENPs) remove SUMO conjugates from substrates [25], and several SENPs...
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báo cáo hóa học:" Activation of PKA, p38 MAPK and ERK1/2 by gonadotropins in cumulus cells is critical for induction of EGF-like factor and TACE/ ADAM17 gene expression during in vitro maturation of porcine COCs" doc
Ngày tải lên :
20/06/2014, 07:20
... and cultured with FSH and/ or LH We previously showed that FSH and LH up-regulate EGF-like factor and Tace/Adam17 mRNA expression, and gonadotropins-induced cumulus expansion and oocyte maturation ... COCs Without FSH and LH: COCs were cultured without FSH and LH for 40 h; With FSH and LH: COCs were cultured with FSH and LH for 40 h; +H89: COCs were cultured with FSH, LH and H89 for 40 h; ... http://www.ovarianresearch.com/content/2/1/20 The binding of FSH and/ or LH in granulosa cells to its own receptors led to rapidly and nongenomic activation of PKA, p38 MAPK, and PI3K in a cAMP-dependent manner [16] and of ERK1/2 via the...
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Báo cáo sinh học: "Compound developmental eye disorders following inactivation of TGF signaling in neural-crest stem cells" doc
Ngày tải lên :
06/08/2014, 18:21
... cornea and lens nutrition and for the regulation of intraocular pressure (Figure 1a-e) Development of these tissues involves coordinated interactions between surface and neural ectoderm and periocular ... endothelial cells and collagensynthesizing keratocytes of the corneal stroma  TGF induces Foxc1 and Pitx2 expression in fibroblasts and in ex vivo eye cultures The absence of Foxc1 and Pitx2 expression ... inactivation of TGF signaling in NC stem cells also leads to cardiac and craniofacial defects and parathyroid and thymic gland anomalies reminiscent of human DiGeorge syndrome [11] Moreover, depending...
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Báo cáo y học: "Targeting Toll-like receptor signaling in plasmacytoid dendritic cells and autoreactive B cells as a therapy for lupus" pps
Ngày tải lên :
09/08/2014, 07:20
... includes myeloid and pDCs, macrophages and MZ-B cells in mice, and pDCs, MZ-B cells and memory B cells in humans, which can respond directly and without priming to both single-stranded and more complex ... only in B cells and plasmacytoid dendritic cells (pDCs) [36] In mice, in addition to B cells and pDCs, myeloid dendritic cells and macrophages also express TLR7 and TLR9 receptors and can respond ... single-stranded CpGDNA [94] Thus, complex TLR9 ligands may need prior processing by DNA-specific enzymes (e.g helicases and/ or topoisomerases) in order to bind more avidly to the TLR9 and to initiate...
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Endofin is a novel component in EGR EGFR oncogenic signaling
Ngày tải lên :
11/09/2015, 10:00
... between L1 and L2 domain while the transmembrane region is between CR2 and juxtamembrane region The abbreviations used: L and CR are for ligand binding and cysteine-rich domains, JM and CT refer ... like, Grb2, Shc and Dok-R that bind to pY1068, pY1086 and pY1148, pY1173 and pY1086, pY1148 respectively, phospholipase PLC-γ which binds to pY1173 and pY992, phosphatase like PTB-1B and SHP-1 are ... target genes STAT1, STAT3 and STAT5 are phosphorylated on Tyr701, Tyr705 and Tyr694 respectively and this phosphorylation mediates their activation and dimerization (Rane and Reddy, 2002) On top...
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The study of interactions of transmembrane receptors and intracellular signaling proteins in live cells by fluorescence correlation and cross correlation spectroscopy
Ngày tải lên :
14/09/2015, 14:25
... applied two fluorescence techniques, i.e fluorescence correlation 10 spectroscopy (FCS) and single-wavelength excitation fluorescence crosscorrelation spectroscopy (SW-FCCS), to study if homo- and heterodimeric ... protein fluorescence activated cell sorting fetal bovine serum fluorescence cross-correlation spectroscopy FER/CIP4 homology fluorescence correlation spectroscopy fluorescein isothiocyanate fluorescence ... function f di fluorescence background focal length of optical lens dimer fraction ρ β f R2 ω and z f G and f R η or cps λ μs μW 2D and 3D coefficient of determination ω and z are the radial and axial...
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Dysfunctional signaling pathway for nitric oxide production in endothelial cells chronically exposed to high glucose or high fatty acids
Ngày tải lên :
15/09/2015, 17:09
... messenger DAG; novel PKCs (δ, ε, η, and θ) are Ca2+-independent and regulated by DAG and phosphatidylserine; and atypical PKCs (ζ and λ) are Ca2+-independent and not require DAG for activation, ... economic and social cost Although diabetes has been recognized for centuries, our understanding of the etiology and pathogenesis of the disease is still incomplete It seems that both genetic and environmental ... diseases and endothelial dysfunction .25 1.3.2 Literature review of mechanisms of high glucose induced endothelial dysfunction 28 1.4 High fatty acids and endothelial dysfunction...
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Expression of neurogranin tagged with enhanced green fluorescence protein in HEK293 cells and its effects on neuronal signaling
Ngày tải lên :
05/10/2015, 22:32
... for gene transfer and expression GFP can be used as the reporter in gene transfer and expression experiments, in which cells expressing GFP are sorted using flow cytometry and expanded for cloning ... UV and blue light with the maximum absorbance peak at 395 nm and a minor peak at 470 nm and emits green light at 509 nm The excitation and emission for enhanced GFP - 23 - (EGFP) is 488 nm and ... Cheryln and I wish to say that without your help and support I could not complete this thesis Lastly, I am grateful to my husband for his encouragement and patience throughout my research and my...
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Expression of neurogranin tagged with enhanced green fluorescence protein in HEK293 cells and its effects on neuronal signaling
Ngày tải lên :
06/10/2015, 20:35
... for gene transfer and expression GFP can be used as the reporter in gene transfer and expression experiments, in which cells expressing GFP are sorted using flow cytometry and expanded for cloning ... UV and blue light with the maximum absorbance peak at 395 nm and a minor peak at 470 nm and emits green light at 509 nm The excitation and emission for enhanced GFP - 23 - (EGFP) is 488 nm and ... Cheryln and I wish to say that without your help and support I could not complete this thesis Lastly, I am grateful to my husband for his encouragement and patience throughout my research and my...
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Báo cáo y học: " Helicobacter pylori induces mitochondrial DNA mutation and reactive oxygen species level in AGS cells"
Ngày tải lên :
25/10/2012, 11:18
... and light strands of the mtDNA, and contains essential transcription and replication elements The D-loop region is a hot spot for mtDNA alterations and the nucleotide positions 16024±16 324 and ... to analyze the mean fluorescence intensity of rhodamine 123 and ROS level can be expressed as the intensity of fluorescence [15] All experiments were repeated for three times and data were expresses ... relationship between ROS and mtDNA mutation rate was assessed using a linear correlation test Results CagA and VacA proteins As shown in Fig 1a and b, the CagA protein (120 kDa) and VacA protein (95...
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Báo cáo y học: "Proteomic analysis of mechanisms of hypoxia-induced apoptosis in trophoblastic cells"
Ngày tải lên :
31/10/2012, 15:12
... fluorescent microscope Fluorescence was mainly observed in cytosol areas in transfected cells FITC-labeled Bag-1 Morpho/AS was transfected into almost all JAR cells by EPEI-mediated transfection (data ... proteins and determined Bcl-2, Bid-21kD, and caspase-8 showed 1.92-fold, 2.21-fold, 2.17-fold, 1.91-fold, 9.40-fold, and 2.76-fold decreases of protein expression, respectively Both decreases and ... Bcl-x-22kD, PTEN, and caspase-7 showed 3.75-fold, 3.31-fold, 2.66-fold, 2.32-fold, 1.91-fold, and 1.86-fold increases of protein expression, respectively On the other hand, smac/DIABLO, Bag-1, and JNK...
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Báo cáo y học: " Low concentration of ethanol induce apoptosis in HepG2 cells: role of various signal transduction pathways"
Ngày tải lên :
31/10/2012, 15:37
... Fas receptor, Fas ligand, FADD and caspase Total RNA was isolated from HepG2 cells using RNAsy kit (Qiagen) and RNA quality was evaluated using Agilent RNA 6000 Nano Chip Kit and Bioanalyzer 2100 ... transcription factor (AP1, SRF, Elk1, Stat1 and NFκB) and members of the Fas receptor signaling pathway (Fas receptor, Fas Int J Med Sci 2006, ligand, FADD and caspase 8) were used Primer design was ... by phosphorylation of GRK2 and PKCα; with values of 109% and 104%, respectively, combined with dephosphorylation of ROKα, PKCδ and PKCμ; with values of -56%, -44% and -28% of control cells, respectively...
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Báo cáo y học: " Mutation Analysis of hCDC4 in AML Cells Identifies a New Intronic Polymorphis"
Ngày tải lên :
31/10/2012, 16:49
... central oncogenes such as c-Jun, Notch, cyclin E and c-myc [3-6] As these oncogenes have all been demonstrated to play roles in AML and MDS [15-18] and the hCDC4 gene features mutations in several ... identified C/T SNP versus the reference sequence (Ref Seq.) and samples lacking the SNP Int J Med Sci 2006, Direct DNA sequencing and mutational analysis Purified PCR products were sequenced ... (Technelysium Pty Ltd, Tewantin, Australia) and GeneDoc software (http://www.psc.edu/biomed/genedoc) Sequences were analyzed in alignment with the NCBI and ENSEMBLE reference sequences of hCDC4:...
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Báo cáo y học: "PKC and PKA Phosphorylation Affect the Subcellular Localization of Claudin-1 in Melanoma Cells"
Ngày tải lên :
03/11/2012, 11:17
... Liebner S., Andbjer B., Kalbacher H., Wolburg H., Haller H., and Fuxe K Organization of choroid plexus epithelial and endothelial cell tight junctions and regulation of claudin-1, -2 and -5 expression ... centrifuged gently, and the lysate was removed from the beads 100 and placed into a new, siliconized microfuge tube PKC substrate antibody was added at a ratio of 1:200, and the lysate and antibody ... Haass N.K., Brandner J.M., Brown E., and Herlyn M Up-regulated expression of zonula occludens protein-1 in human melanoma associates with N-cadherin and contributes to invasion and adhesion Am...
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