dyskerin and its role in telomeric maintenance

... very-KIND-MAP2 interaction v-KIND KIND2 KIND2-1 KIND2-2 KIND2-3 KIND2-4 KIND2-5 A 620 521 555 488 589 EGFP EGFP-KIND2-1 EGFP-KIND2-2 EGFP-KIND2-3 EGFP-KIND2-4 EGFP-KIND2-5 EGFP-KIND2 EGFP-KIND1 ... the structural and functional determinants of the protein–protein interaction between v-KIND and MAP2 (i.e the MAP2-binding core module in v-KIND and the v-KIND-binding core module in MAP2) across ... deletion of RasGEF; KIND1, KIND1 domain; KIND2, KIND2 domain (B) KIND2 domain anchors v-KIND to dendrites Flag-tagged v-KIND, DKIND1, DKIND2, DKIND1 + 2, DRasN, DGEF, KIND1 or KIND2 together with...

Ngày tải lên: 14/02/2014, 19:20

... wild-type occludin (OccWT) and variant occludin in apoptosis and invasion, as determined by assay, we revealed that exon played a major role in the induction of mitochondria-mediated apoptosis and the ... occludin in apoptosis and invasion A C B D E F Fig Identification of a human occludin splice variant in liver cell lines (A) Occludin protein expression was examined with an antibody to the N-terminus ... cells In Chang cells, stained occludin was observed in the cytoplasmic region Additionally, in OccWT-overexpressing Hep3B and Huh7 cells, regions stained with antibody to hemagglutinin (HA), indicating...

Ngày tải lên: 18/02/2014, 18:20

... copper binding could still allow domain swapping to occur It is of interest that a N-terminally truncated prion protein, lacking the copper binding domain is capable of domain swapping and forms ... C-terminus (box in Fig 1) at sites: 92, 75, 66 and 58 As histidine residues are central to copper binding in many proteins they probably form part of the copper binding sites in this protein Although ... binding on the secondary structure was determined using CD in the far UV To prepare proteins without Cu2+, part of each protein 4252 Fig Comparison of copper binding by stefin A and stefin B using...

Ngày tải lên: 19/02/2014, 06:20

... in the AUH gene This indicated that AUH, in addition to its RNA binding function, must play an important role in leucine catabolism AUH was overproduced in E coli and characterized by measuring ... (c.719C>T) in exon and an insertion mutation c.613–614insA This insertion causes a frameshift that starts at Met205 and leads to the introduction of a stop codon after four amino acids The intra-assay ... Characterisation and mitochondrial localisation of AUH, an AU-specific RNA-binding enoyl-CoA hydratase Gene 228, 85–91 Nakagawa J & Moroni C (1997) A 20-amino-acid autonomous RNA-binding domain contained in...

Ngày tải lên: 19/02/2014, 07:20

... (Michaelis constant) and Vmax (maximum velocity) values were determined by incubating the diluted recombinant proteins in the standard reaction mixture in the presence of increasing concentrations ... were removed and minced with a surgical knife The minced tissue was wrapped in cotton gauze and suspended in NaCl/Pi containing 100 lgÆmL)1 penicillin/ streptomycin at 37 °C for h After incubation ... were incubated at 37 °C in a humidified 5% CO2 incubator in the absence (control) and presence of increasing concentrations of inhibitors for 10 days, and the number of molting larvae was determined...

Ngày tải lên: 20/02/2014, 11:20

... proteins, Vpr, has been shown to dysregulate several host cellular functions including apoptosis in infected and uninfected bystander target cells through its interaction with host cellular proteins ... Oncogenes and Related Genes Shanghai Cancer Institute, Shanghai, China Smith RK, Carroll PM, Allard JD & Simon MA (2000) MASK, a large ankyrin repeat and KH domain-containing protein involved in Drosophila ... ankyrin repeat proteins, using the consensus established by Kohl et al [10] and Mosavi et al [11], which contains ankyrin repeats from over 4000 proteins, indicated that the ankyrin repeat domains...

Ngày tải lên: 07/03/2014, 21:20

... Printing Office, Washington, D.C., 2004 SOLAR AND SPACE PHYSICS AND ITS ROLE IN SPACE EXPLORATION the fundamental role of solar and space physics research both in scientific exploration and in ... principal operating agency of both the National Academy of Sciences and the National Academy of Engineering in providing services to the government, the public, and the scientific and engineering ... Engineering was established in 1964, under the charter of the National Academy of Sciences, as a parallel organization of outstanding engineers It is autonomous in its administration and in the...

Ngày tải lên: 14/03/2014, 10:20

... no membrane-spanning domains in PEX14 have been unambiguously identified and it was observed in mammalian cells and S cerevisiae that both the N-terminal and the C-terminal domain are at the cytosolic ... synthesized proteins in the cytosol due to the inability to import matrix proteins by the fraction of growing and dividing glycosomes The miscompartmentation of PTS-1 and PTS-2 proteins (GAPDH and ALD, ... approximately 0.5 to induce the expression of the protein and growth was continued for h Cells were collected by centrifugation (3000 g, 10 min, °C) and resuspended in cell lysis buffer containing 50 mM...

Ngày tải lên: 23/03/2014, 17:21

... Available online http://arthritis-research.com/content/6/1/26 known roles in tissue inflammation, and damage in innate immunity Besides its capacity to drive the production of IFN-γ and IL-2 by ... development of inflammatory arthritis [14-16] This highlights the critical role of IL-1ra in the constitutive maintenance of peripheral tolerance, and in counterbalancing the proinflammatory effects ... Stoll S, Germain RN: Imaging of T-cell interactions with antigen presenting cells in culture and in intact lymphoid tissue Immunol Rev 2002, 189:51-63 O’Sullivan B, Thomas R: CD40 and dendritic...

Ngày tải lên: 09/08/2014, 01:23

... loss during intestinal tumorigenesis may, in part, act through upregulation of b-catenin, increasing intestinal b-catenin signaling and cell proliferation Also, increase in small-intestinal mucosa ... regulating cell cycle genes and interacting with key DNA damage-activated checkpoint proteins Per1 overexpression in cancer cells increases ionizing radiationinduced apoptosis, whereas inhibition ... regulating the circadian clock include acetylation, phosphorylation, ubiquitination and sumoylation In terms of phosphorylation, Casein kinase epsilon (CK1ε) and Casein kinase delta (CK1ε and CK1δ),...

Ngày tải lên: 10/08/2014, 09:20

... also increasingly involved in the development and delivery of a range of education and training programmes designed to help individuals working in HIV advance professionally, from administering ... Providing education and training opportunities for its membership is important for any professional society, and the IAS is looking at ways to strategically expand its role in this area in order ... cross-disciplinary linkages and dialogue, and to strengthen the focus on youth – began to be implemented in the planning for the XVI International AIDS Conference (AIDS 2006) in Toronto and will...

Ngày tải lên: 13/08/2014, 09:20

... bacitracin, a PDI inhibitor Finally, PDI expression was induced by the antiinflammatory cytokine IL-10, and IL-10-mediated inhibition of LPS-induced IL-6 expression was reduced by bacitracin These findings ... molecular chaperones in the folding of oxidized proteins Refolding of colloidal thyroglobulin by protein disulfide isomerase and immunoglobulin heavy chain-binding protein J Biol Chem 2001, 276:21337-21342 ... cytokines play a major role in sepsis-induced liver injury [19,20], we also investigated the expression of PDI in cells of the macrophage-like cell line, RAW 264.7, after incubation with LPS In...

Ngày tải lên: 13/08/2014, 11:22

... transduced into OV90 cells 103 xiv Figure 33 Wild-type TG2 and N-terminal fibronectin binding domain of TG2 induce EMT in OV90 cells Figure 34 104 Wild-type TG2 and N-terminal fibronectin binding domain ... at its protease sensitive hinge region, which is between the N-terminal ECM binding domain and the C-terminal LAP binding domain This can be achieved by many proteinases in the serine proteinase ... Tyr583 are involved both in GDP and ATP binding to TG2 The residue Arg580, which is important for GTP/GDP binding, is not involved in ATP binding (Han et al 2010) By purifying the insulin-like growth...

Ngày tải lên: 24/08/2014, 10:58

... expressed predominantly in erythroid cells HRI is regulated by heme through the two heme-binding regions in HRI: an N-terminal domain of HRI and in an insert region in the protein kinase domain of HRI ... the initiation codon in underline and bold and a flanking purine at the -3 and a G at the +4 positions (15) Together the eIF1 and eIF1A facilitate the recognition and selection of initiating ... cytoplasmic splicing of mRNA encoding the Xbox binding protein1 (XBP1) in mammals and homologue of the mammalian activating transcription factor/cAMP-response element (CRE)-binding protein (HAC1) in yeast...

Ngày tải lên: 24/08/2014, 12:32

Ngày tải lên: 14/11/2014, 06:30

... knockdown MIN6 cell line Figure4.2 Increased betagranin storage in BIG3-deficient MIN6 cells Figure4.3 Increased insulin storage in BIG3-deficient MIN6 cells ix Figure4.4 Increased betagranin secretion ... is more predominant ( Reaven, 1993; Ferrannini, 1998; Relimpio, 2003) The balance between insulin action and insulin secretion is coordinated by insulin sensitive tissues and endocrinal beta cells ... protein recruitment distiguishes the cargo-loaded subdomains from the rest of TGN Curvature-inducing proteins, such as the Bin/amphiphysin/Rvs (BAR) domain family and the GRIP-domain-containing...

Ngày tải lên: 11/09/2015, 09:15

... acid-binding adhesin The sialic acid-binding adhesin is a 70 kDa protein that belongs to the large Hop family of H pylori outer membrane protein, including BabA sabA is found to be present in most ... genes coding for a range of pro-inflammatory mediators, including interleukin-6, interleukin-8, and tumor necrosis factor α (Schreck et al., 1991) The increased expression of these pro-inflammatory ... and KATO III) displayed an increase in H2O2 generation, activated NF-κB and IL-8 production However, preincubating these cells with NAC (an antioxidant), ST638 (a tyrosine kinase inhibitor) and...

Ngày tải lên: 12/09/2015, 09:55

... is also expressed in appreciable levels in the spleen and intestine Immunohistochemistry analysis revealed that in the mouse embryonic brain, Rab22B labeling is found in nestin and RC2 epitope-positive ... act in a more indirect manner Thus, Rab11, besides playing important roles in retinal development of the eye and embryogenesis, also appears to have a specific role in neuronal and neuroendocrine ... form myelin sheaths (Simons and Trajkovic, 2006) The microglia is the equivalence of 24 Introduction immune cells in the CNS, playing roles in CNS inflammatory responses and serving in phagocytotic...

Ngày tải lên: 12/09/2015, 09:55

... According to the type of amino acid phosphorylated, the protein kinases can be divided into two subgroups: protein serine/threonine kinases and protein tyrosine kinases Serine/threonine protein kinases ... called intracellular signaling proteins including G proteins, small GTPase and protein kinases13-15 5 1.2 Protein kinases 1.2.1 History and Definition The presence of phosphorus in proteins has ... serine/threonine kinases A number of residues within this domain are highly conserved between both types of protein kinases, but the tyrosine kinases and serine/threonine kinases are distinguished...

Ngày tải lên: 12/09/2015, 21:10

... function in the brain BGT-1 is capable of transporting both betaine and GABA, as examined in Xenopus oocytes and various cell lines But the physiological substrate of BGT-1 in the brain remains to ... decrease in GAT-1 and GAT-3 immunostaining, but no change in GAT-2 staining, was observed in the degenerating CA subfields In contrast, increased BGT-1 immunoreactivity was observed in astrocytes ... immunoreactivity in neurons and little induction of BGT-1 in astrocytes, a marked increase in BGT-1 immunoreactivity was observed in astrocytes in the degenerating CA fields and fimbria, in rats injected...

Ngày tải lên: 16/09/2015, 15:55