dynamic response analysis of dental implant bone
Functional studies of a type III and a novel secretion system of edwardsiella tarda
... Survey of the ECP profiles of E tarda and its mutants .128 Fig V.3 Identification of EvpI with MALDI-TOF MS 130 Fig V.4 Identification of EssA with MALDI-TOF MS 131 Fig V.5 ECP profile ... Fig III.3 Proteome analysis of E tarda PPD130/91 and the ΔesrC mutant 64 Fig III.4 Transcription of eseD was reduced by the mutation of esrC .65 Fig III.5 Effect of loss -of- function mutants ... V.3.1 Sequence analysis of EVP gene cluster 122 V.3.2 Systematic mutation of individual genes of the EVP secretion system 126 V.3.3 Identification of secreted proteins of the EVP secretion...
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plasmids and vectors - Instructor Supplement to pGlo Bacterial Transformation
... year (1976) before the first “safe” (EK2 category) line of E coli was released That year, RAC released a set of guidelines requiring the use of safe bacteria NIH Guidelines Self Regulation in ... levels 1994 officially relinquished control of GMO plants in the environment to EPA and APHIS The First “Safe” Bacterium Released in 1976 by Roy Curtiss III at the University of Alabama E ... Plasmid(ology) The inclusion of polylinkers into plasmid vectors Polylinker is a tandem array of restriction endonuclease sites in a very short expanse of DNA For example, pUC18’s polylinker...
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Báo cáo Y học: Structural analysis of deacylated lipopolysaccharide of Escherichia coli strains 2513 (R4 core-type) and F653 (R3 core-type) pot
... assignment of H-7a/b and C-7 of L,D-Hepp residues F and H The chemical shift of H-6 of residue F could also be assigned in the latter spectrum The chemical shift of the H-6 proton of residue ... core-oligosaccharides of the R1 and R2 core-types [8] whereas the chemical structure of the inner core-region of the E coli R4 core-type was hitherto unknown The chemical structure of the outer core region of the ... based conformational analysis of the inner core region of enterobacterial LPS and epitope mapping of WN1 222-5 we have in addition isolated the complete core-oligosaccharides of E coli F653 (R3-core)...
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Maltose and maltotriose transport into ale and lager brewer´s yeast strains docx
... Vidgren Division of Genetics Department of Biosciences Faculty of Biological and Environmental Sciences University of Helsinki, Finland A dissertation for the degree of Doctor of Philosophy to ... group by a loss of large portion of S cerevisiae genome whereas in group the loss of the S cerevisiae portion of the genome was minor (Dunn and Sherlock, 2008) The loss of portions of the S cerevisiae ... levels of genes, stability of the transporters in the plasma membrane, etc so that the number of transporters per g of yeast in each case is not known 28 Introduction Table Substrate range of -glucoside...
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Báo cáo hóa học: " In vitro permissivity of bovine cells for wild-type and vaccinal myxoma virus strains" potx
... collected Analysis was performed using CellQuestPro and Flowjo Software A Cells were infected at m.o.i of (T1-Serp2-GFP or SG33-GFP) and collected 16 h p.i Results shown are representative of three ... correspond to a mean of at least two independent experiments Error bars correspond to the standard error of the mean B Rabbit and bovine cells were infected with the T1-TK::LacZ (m.o.i of 0.1 and 1) ... viral passages analysis and infection with both T1 and SG33 expressing LacZ gene showed that these cells failed to support spread of either MYXV strain Electron microscopy study of BT-infected...
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Báo cáo hóa học: " Nucleotide mismatches between the VP7 gene and the primer are associated with genotyping failure of a specific lineage from G1 rotavirus strains" potx
... out of 121 G1 strains from lineage I showed the four specific mismatches at the 5' end of the 9T1-1 primer This was associated with the failure of genotyping of G1 strains from Bangladesh Two of ... recent years it was reported that the accumulation of point mutations in VP4 and VP7 genes was the main cause of the failure of the G or P-typing of rotavirus strains [7,8,12,16-21] At least, there ... methods with Kimura 2-parameter as a model of nucleotide substitution with the MEGA 3.1 [26] and Phylip v3.65 softwares The statistical significance of the tree was preformed by bootstrapping,...
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Screening of Bacillus strains as potential probiotics and subsequent confirmation of the in vivo effectiveness of Bacillus subtilis MA139 in pigs doc
... for 24 h, the isolates were killed by exposure to chloroform vapor for 30 The chloroform was then evaporated for 20 0.1 ml of a 24 h culture of E coli K88 in Nutrient Broth (about 108 CFU/ml) was ... spore viability The spore viability of the six Bacillus strains when cultured in simulated gastric fluid with a pH of 2.0 is shown in Table Five of the spores of six strains ± ± ± ± ± ± 0.6 1.0 ... of a pig The fact that the viable counts of most of the six Bacillus strains did not decrease after h of incubation suggests that a reasonable percentage of these Bacillus strains, if provided...
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Probiotic Lactobacillus Strains: in vitro and in vivo Studies pot
... (Warsaw), the grant IAA500200710 of the Grant Agency of the Academy of Sciences of the Czech Republic, by grant 2B06053 of the Ministry of Education, Youth, and Sports of the Czech Republic and by ... Experimentation Ethics Committee of the Institute of Microbiology, Academy of Sciences of the Czech Republic and conducted in accordance with the European Convention for the Protection of Vertebrate Animals ... J., ROSOVA V.: Effect of oral application of a probiotic E coli strain on the intestinal microflora of children of allergic mothers during the first year of life Folia Microbiol 52, 189–193 (2007)...
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Báo cáo khoa học: "Capsular polysaccharide typing of domestic mastitis-causing Staphylococcus aureus strains and its potential exploration of bovine mastitis vaccine developmen" docx
... (pH 6.0) at a flow rate of 0.5 ml/min The sample was applied in 1.5 ml of buffer, and ml of each fraction was collected Fig Distribution of CP concentration by serotypes of randomly selected 30 ... digested overnight at 37oC with 100 mg/ml of DNase, 100 mg/ml of RNase, and 100 mg/ml of lysostaphin (Sigma), followed by overnight digestion at 37oC with 100 mg/ml of pronase After dialysis and lyophilizing ... Regardless of the colony morphology, the strains of type and type 336 expressed CP as determined by ELISA inhibition However, the Table Colonial morphology of S aureus from 107 isolates in serum soft...
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Báo cáo khoa học: "Primary structure of mature SAG1 gene of an Indonesian Toxoplasma gondii and comparison with other strains" pps
... neht nim 01 rof Co27 ta pets noitagnole lanoitidda na yllanif ;ces 03 nim rof Co27 ta noitagnole dna ,ces 04 rof Co06 ta gnilaenna ,ces 04 rof Co59 ta noitarutaned fo selcyc 03 ;nim rof Co49 ta ... eht fo yna ni dnuof ton erew )G( ggg dna )A( gcg e.i ,snoitairav rehto owt ehT desu saw )E( gag ro )V( gtg nodoc ylno ,79 noitisop ta elpmaxe rof ,ylgnidroccA devresbo erew ,ruof elbissop eht ... )528156YA oN ccA( repap siht ni ecneinevnoc rof -SI dellac evah ew taht etalosi amsalpoxoT naisenodnI eht fo 1GAS erutam fo ecneuqes lautca eht erofereht si dehsilbatse ecneuqes ehT deniatbo...
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Báo cáo sinh học: "Molecular epidemiology of clinical and carrier strains of methicillin resistant Staphylococcus aureus (MRSA) in the hospital settings of north India" pptx
... Table 2: Antibiotic resistance profiles of 61 strains of MRSA isolated in an orthopaedic surgical ward Pattern Antibiotic resistance profiles of MRSA No of resistance markers Strains 10 11 12 ... JMR-7, 10, 47, 49, 57 case of nasal swabs of hospital workers and patients (n = 225) the frequency of MRSA isolates was 28.9% that is equivalent to MRSA carrier rate of 23.1% Of the 76 MRCNS studied ... (18.4%) and nasal swabs of patients having ocular infections (14.5%), where as 10.5% of MRCNS were isolated from ocular swabs of hospital workers Resistance Profiles of Staphylococcal Isolates...
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Báo cáo sinh học: "n vitro activity effects of combinations of cephalothin, dicloxacillin, imipenem, vancomycin and amikacin against methicillin-resistant Staphylococcus spp. strains" pdf
... (FICA) = MIC of drug A in combination/MIC of drug A alone, and the FIC of drug B (FICB) = MIC of drug B in combination/MIC of drug B alone If the MIC of any agent alone occurred at the lowest ... aminoglycoside combination (indifferent effect) One of the main obstacles to generalize the concept of the usefulness of antimicrobial combinations is the diversity of combinations in published studies [24-26] ... checkerboard fashion on the day of the assay Each well contained 0.1 mL of individual antimicrobial combinations Suspensions with turbidities equivalent to that of a 0.5 McFarland standard were...
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Comparative genome and phenotypic analysis of Clostridium difficile 027 strains provides insight into the evolution of a hypervirulent bacterium" ppsx
... proportion of the 027-specific genes, with two-component regulators and 15 other transcriptional regulators One of the most striking regions of genetic difference was an additional complete copy of the ... Bonne L, Lalande V, Burghoffer B, Luiuz R, Petit JC: Clinical features of Clostridium difficile-associated infections and molecular characterization of strains: results of a retrospective study, ... Purification of two high molecular weight toxins of Clostridium difficile which are antigenically related Microb Pathog 1987, 2:307-318 Bouvet PJ, Popoff MR: Genetic relatedness of Clostridium...
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Báo cáo y học: "Pre-clinical development as microbicide of zinc tetra-ascorbo-camphorate, a novel terpenoid derivative: Potent in vitro inhibitory activity against both R5- and X4-tropic HIV-1 strains without significant in vivo mucosal toxicity" docx
... average scores of to receive a vaginal irritation rating of "minimal," scores of to receive a vaginal irritation rating of "mild," scores of to 11 receive a vaginal irritation rating of "borderline," ... vaginal application [16] Statistical analysis Statistical significance of the treated group mean with that of control group was analyzed by a way -analysis of variance, followed by Dunnett's multiple ... due to the effect of C14 on cellular expression of HIV-1 receptors and co-receptors, but rather the direct effect of C14 on the integrity of viral particles or the inhibition of viral entry In...
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Báo cáo y học: " Comparative analysis of cell culture and prediction algorithms for phenotyping of genetically diverse HIV-1 strains from Cameroon" pps
... HIV epidemic in Cameroon, we analyzed viral sequences of the strains to identify genetic subtype Phylogenetic analysis of partial sequences of gp41, p17 and pol region revealed that 53% belonged ... that majority of viruses were recombinants of CRF02_AG with gene segments of other HIV CRFs and subtypes suggesting that newly emerging HIV strains in Cameroon may be recombinants of the predominant ... these findings and to understand the complexity of the emerging diversity of HIV in this region of high diversity Finally, although higher frequencies of drug resistance in patients harboring X4...
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Báo cáo khoa hoc:" Comparison of nanoparticle-mediated transfection methods for DNA expression plasmids: efficiency and cytotoxicity" potx
... μL of each sized AuNPs were incubated for 24 h at room temperature with μg of pIRES-hrGFPII-eIL-12 or pIREShrGFPII-rHMGB1 at a total volume of 95 μL of ddH2O For transfection μL aliquots of FHD ... transfection: (MA Lipofection & MATra-A): 4.1) MA Lipofection: μL of FHD (Roche, Mannheim, Germany) were added to μg of pIRES-hrGFPII-eIL-12 or pIRES-hrGFPII-rHMGB1 to a total volume of 97 μL ddH2O ... plot analysis and compared to cells incubated only with transfection reagent with or without the addition of NPs (depending of the protocol used) Page 10 of 11 Proliferation Assay Proliferation of...
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báo cáo khoa học: "Fatty acid profiles and their distribution patterns in microalgae: a comprehensive analysis of more than 2000 strains from the SAG culture collection" docx
... (Non-Parametric Multivariate Analysis of Variance, Analysis of Similarity) and visualised as ordinations from multigroup discriminant analysis (Canonical Variates Analysis) The percentages of dissimilarity ... systematic analysis has been carried out on a large scale basis on either the profiles of lipids or FAs in microalgae Therefore, we determined the FA profiles of all available microalgal strains of the ... characterisation of FA profiles of the SAG microalgal strains was performed by screening long chain FAs (C-14 - C-24) esterified within lipids A total of 2076 culture strains from the SAG (equal 91% of the...
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Báo cáo y học: " Antigenic analysis of classical swine fever virus E2 glycoprotein using pig antibodies identifies residues contributing to antigenic variation of the vaccine C-strain and group 2 strains circulating in China" ppt
... Hydrophobicity profile and evolution analysis within antigenic units of E2 Hydrophobicity profile was generated using DNASIS software by the method of Kyte and Doolittle [44] Evolution analysis was ... http://www.virologyj.com/content/7/1/378 Page of 14 A B Figure Generation of prokaryotic-derived recombinant (rE2) proteins (A) Schematic presentation of expression of truncated rE2 proteins of CSFV The antigenic domain of glycoprotein ... (Figure 5C) Analysis of codon and amino acid diversity in the antigenic units of E2 To get more insight into antigenic and genetic evolution of the antigenic units, the diversity of codon and...
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Báo cáo y học: "Genetic diversity and C2-like subgenogroup strains of enterovirus 71, Taiwan, 2008" pptx
... http://www.virologyj.com/content/7/1/277 Page of 11 Figure Different subgenogroups of 989 enterovirus 71 (EV-71) isolates in Taiwan in 2008 according to the BLAST results The subgenogroup was determined by BLAST analysis of partial ... Phylogenetic analysis of enterovirus 71 strains based on partial VP1 gene sequence (nucleotide position 16-418) Phylogenetic analysis was performed based on partial VP1 gene nucleotide sequences of reference ... titers of the antisera for different genogroups of EV-71 ranged from 512 to >1,024, while the titers of the antisera for homologous EV-71 isolates were >1,024 [33] The antigenic diversity of subgenogroup...
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Báo cáo y học: " A multiplex reverse transcription-nested polymerase chain reaction for detection and differentiation of wild-type and vaccine strains of canine distemper virus" pptx
... http://www.virologyj.com/content/7/1/86 Page of Results Phylogenetic analysis based on H gene Determination of the application conditions of the multiplex RT-nPCR A phylogenetic tree based on the H genes of various CDV strains ... sequenced, and the sequences were aligned with the H genes of a number of CDV strains available in GenBank using the MegAlign function of the DNAStar software package Thirty field samples from dogs, foxes, ... inter- and intra-assay replicates of the multiplex RT-nPCR gave consistent results, indicating the repeatability of the assay Figure Amplification of genomes of different easily infected canine...
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Tìm thêm: xác định các mục tiêu của chương trình xác định các nguyên tắc biên soạn khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản khảo sát chương trình đào tạo gắn với các giáo trình cụ thể xác định thời lượng học về mặt lí thuyết và thực tế tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra đối với đối tượng giảng viên và đối tượng quản lí khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam nội dung cụ thể cho từng kĩ năng ở từng cấp độ phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ lồng sóc mở máy động cơ rôto dây quấn hệ số công suất cosp fi p2 đặc tuyến hiệu suất h fi p2 đặc tuyến dòng điện stato i1 fi p2 động cơ điện không đồng bộ một pha thông tin liên lạc và các dịch vụ từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008