drugs that inhibit signalling pathways for tumor cell growth and proliferation

... silence and Cell growth arrest ALCL cells Figure Development of a tumor cell type-selective and cancer gene-specific nanocomplex for ALCL cells A, A nano-sized carrier core structure was initially formed ... therapeutic agent by formulating a nanocomplex that is both tumor cell- selective and cancer gene-specific for ALCL The nanocomplexes are specific and noncytotoxic to lymphoma cells, which advance ... monitored As before, exposure of Karpas 299 cells and Jurkat cells to the nanocomplexes had no effect on cell viability (Figure 6C) Moreover, cells were treated with the nanocomplexes for luciferase...

Ngày tải lên: 11/08/2014, 00:22

... their ligand properties for purified lectins, for tumor cells in vitro, and in tissue sections, and their biodistribution in tumor- bearing mice Bioconjugate Chem 8, 845–855 ´ 13 Unverzagt C, Andre ... this level of testing The decrease of cell positivity for KG-1 cells and rather constant results for K-562 cells can be judged as internal controls for cell- type specificity in the direct comparison ... lectin ligand cell surface binding was performed with the following human tumor lines: Croco II (B-lymphoblastoid cell line), CCRF-CEM (T-lymphoblastoid cell line), K-562 (erythroleukemia cell line),...

Ngày tải lên: 30/03/2014, 16:20

... Extra -and intracellular Muc16 expression by MOVCAR cells Extra -and intracellular Muc16 expression by MOVCAR cells (A) MOVCAR-10 cells were labeled with 618F (grey line) and analyzed for cell surface ... the cell surface and shed from the cell in soluble forms Muc16, on the other hand, is detected primarily in the spent media and in the intracellular environment This observation indicates that ... ovarian tumor cells with Tris buffered saline containing 0.5% Triton X-100 and a cocktail of protease inhibitors (Sigma) For Western blotting, 25 μg of protein was separated on 7.5% SDS-PAGE gels and...

Ngày tải lên: 20/06/2014, 07:20

... Double-knockout mice for EGF and TGF, but which contained AREG, displayed normal ductal growth and TEB formation Mice that were single-knockout for AREG or a triple-knockout for all ligands displayed ... of to cell layers, including cap cells that make up the ‘basal’ layer and multiple layers of interior luminal-like body cells that line the duct Signaling within the cells of the TEBs and the ... mammary ductal growth This suggests that EGF and TGF are dispensable for mammary gland growth, while AREG plays a vital role in glandular development Recombination grafts indicated that estrogen...

Ngày tải lên: 27/06/2014, 17:20

... weeks: (A) PHB, (B) PHBV(5%) and (C) PHBV(8%) Size of the scale bar of A1, B1 and C1 is 100 µm and that of A2, B2 and C2 is 10 µm 94 xv Fig 4.37 SEM scans of cell- cell and cell- substrate interactions ... primary cells were subcultured into cell lines The proliferation rate of the cell lines is much higher than that of primary cells, which therefore enables maintaining of the cells in vitro for longer ... candidates for tissue engineering In addition, they have been proven to support cell growth and proliferation However, PHB and PHBV microspherical scaffolds has yet to be studied for liver cell...

Ngày tải lên: 06/10/2015, 20:43

... engineering Cell behavior includes cell adhesion, cell spreading, cell migration and cell morphology Cell seeding -The methods of seeding cells onto surfaces are categorized into static form and dynamic ... biodegradability and surface properties Firstly, the ideal structure of scaffold is to have an open and ample space for cell growth and proliferation [2], and porous and three dimension form to maximize ... makes the stem cell decide to become a particular cell type and differentiate is still under investigation 2.2.4 Cells seeding and cell behaviors The methods of cell seeding and cell behaviors...

Ngày tải lên: 22/10/2015, 21:14

... manner We also show that Dm-eIF4B is required for cell survival and that it stimulates cell proliferation Experimental procedures Construction of plasmids PCR amplification was performed on the EST ... protein and 100 000 c.p.m.ÆlL)1 RNA (previously treated for 15 at 70 °C) The reaction mixtures were incubated for 15 at room temperature and then irradiated for 35 on ice at 254 nM and digested for ... respective 3¢-UTRs (62 nucleotides for the long and 409 nucleotides for the short form) The ORFs predict a Dm-eIF4B-L isoform of 459 amino acids (52.2 kDa) and a Dm-eIF4B-S isoform of 390 amino acids (44.2...

Ngày tải lên: 07/03/2014, 15:20

... same for all eukaryotic cells? For yeast cells, it has been shown, by blocking cell- cycle progression and measuring cell growth rate, that big cells grow faster than small cells [11] Thus, for ... Schwann cells coordinate cell growth and cell- cycle progression so differently? The lifestyles of yeasts and animal cells are crucially different As a unicellular organism, each yeast cell grows and ... growth can shorten the cell cycle in Schwann cells [2] This finding suggested that yeasts and Schwann cells might use different strategies to coordinate cell growth and cell- cycle progression...

Ngày tải lên: 06/08/2014, 18:20

... increases colony formation and tumorigenesis of IMR-32 cells, but decreases colony formation and tumorigenesis of CP70 cells Taken together, our results show that Trip10 expression in brain tumors, breast ... (top and middle left panels) and tumor growth in nude mice (bottom left) (B) In contrast, Trip10 overexpression in CP70 cells suppressed colony formation (right top and middle panels) and tumor growth ... and IMR-32 cells demonstrated that Trip10 is associated with H3K4me3, but not H3K27me3 Trip10 can be oncogenic or tumor suppressive, increasing IMR-32 cell proliferation and inhibiting CP70 cell...

Ngày tải lên: 10/08/2014, 05:21

... preincubation at 94°C and cycles at 94°C for 45 s, 45°C for 45 s, and 68°C for min, followed by 30 cycles at 94°C for 45 s, 55°C for 45 s, and 68°C for min, and by cycle at 72°C for The recombined ... the third cell division (D) Cell line Lower semicell formed after the first, upper semicell after the second cell division (E) Cell line 18 Upper semicell resulting from the first cell division, ... involved in cell wall formation in green algae and land plants Cell walls have played crucial roles in the colonization of land by plants [63,64] For a detailed understanding of how cell walls...

Ngày tải lên: 11/08/2014, 11:21

... components and conditions for hNESPCs growth and differentiation in vitro Primary cells from biopsies survived and more than 90% of cells proved to have properties of epithelial stem or progenitor cells ... by four types of cell: basal cells, goblet cells and ciliated or non-ciliated columnar cells Basal cells may involve into the epithelial repair after injury and other types of cells are more related ... dynamic and complex stem cell niche One way to distinguish between these alternatives would be to isolate and grow stem cells from healthy and diseased tissues and compare them under conditions that...

Ngày tải lên: 09/09/2015, 11:29

... ion channels and may also direct second messenger production via GPCR coupling Ras and Rho are small, monomeric G proteins that couple RTK and GPCR activation to cell growth and cell dynamics ... cell dynamics and motility 63 1.2.6.2.1 Roles of Rho GTPases in cell migration 63 1.2.6.2.2 Roles of Rho GTPases in phagocytosis 67 1.2.6.3 Rho GTPases in cell proliferation, transformation and ... development 1.2.6.3.1 Cell proliferation and cell cycle progression 68 1.2.6.3.2 Roles of Rho GTPases in gene expression 69 1.2.6.3.3 Role of Rho GTPases in cellular transformation 70 and cancer 1.2.6.3.4...

Ngày tải lên: 16/09/2015, 08:31

...  32  1.7.5 Epimedium flavonoid and cancer cell proliferation . 32  1.8  Icaritin and cancer cell proliferation 36  1.8.1 Icaritin and cancer cell proliferation  36  1.8.2 ... the proliferation of HepG2 cells in a concentration dependent manner; icariin also inhibited the tumor growth in HepG2 bearing NMRI nu/nu mice The tumor weight was inhibited by 55.6% and tumor ... stimulatory and inhibitory effects on breast cancer cell proliferation E sagittatum and icariin E sagittatum extract inhibited the proliferation of all cell lines (SK-Hep1, PLC/PRF/5, K562, U937, P3H1 and...

Ngày tải lên: 04/10/2015, 17:05

... optimizing cell growth and paclitaxel accumulation via callus and cell suspension cultures ii) to enhance paclitaxel production using precursors and abiotic elicitors For optimization of cell growth, ... Wickremesinhe and Arteca, 1994), MS (Murshige and Skoog, 1962) and Woody Plant Medium (WPM) (Loyd and McCown, 1981) have been used for the initiation, proliferation and maintenance of the callus and cell ... inorganic salts were used as basal media for callus formation and maintenance; and then the best one for callus growth was used for all next experiments The formation and composition of MS, B5, WP media...

Ngày tải lên: 23/10/2015, 15:38

... parental SNARE proteins for SNARE complex formation, inhibiting SNARE assembly In an initial attempt to search for the potent peptide sequences that inhibit SNARE complex formation and neurotransmitter ... Fig 4, we show that SCN, SynN and VpN inhibited N-terminal zippering and that the already-zipped complex was not inhibited by those peptides (Fig 4A,B) Together with the fact that N-terminal ... SCN, SynN and VpN inhibited SNARE complex formation significantly (Fig 3B,C) VpN inhibited SNARE complex formation with a similar efficiency to verapamil N-Terminal-mimicking peptides inhibited...

Ngày tải lên: 30/03/2014, 04:20

... DCs with HIFU-treated tumor cells and assay for their maturation status × 10 immature DCs generated from mouse bone marrow cells were co-cultured with HIFU-treated B16 tumor cells at ratio of 1:1 ... cause of failure for HIFU therapy[10,12], indicating the need to augment the host anti -tumor immunity Therefore, the optimized strategies that can reduce the primary tumor mass and elicit simultaneously ... antigen-presenting cells (APCs) that capture antigens in peripheral tumor tissues and migrate to secondary lymphoid organs, where they cross-present the captured antigens to T cells and activate them[14]...

Ngày tải lên: 18/06/2014, 16:20

... suppresses growth and causes cell cycle arrest of RPMI8226 myeloma cells PHI suppresses growth and causes cell cycle arrest of RPMI8226 myeloma cells (A) PHI suppresses growth of RPMI8226 myeloma cells ... for 15 min; then 40 cycles of 95°C for 15 sec, 60°C for 30 sec, 72°C for 30 sec; and finally 10 at 72°C At least two independent PCR amplifications were performed for each sample Cell cycle and ... Student's t-test for statistical difference Results PHI inhibits proliferation and causes G1 arrest of multiple myeloma cells To evaluate the effects of PHI on the growth of myeloma cells, the multiple...

Ngày tải lên: 10/08/2014, 22:20

... lines and a normal breast cell line .46 Figure 3.1.2 Growth inhibition rate of the combination of TQ andchemotherapeutic drugs .47 Figure 3.1.3 Effects of TQ on cell cycle progression and cell ... TQ inhibited forestomach tumor incidence and multiplicity in benzo(a)pyrene-induced forestomach tumor mouse model (Badary et al., 1999) Moreover, TQ was found to inhibit the growth of various tumor ... investigated both in vitro and in vivo TQ was found to inhibit the growth of MCF-7, MDA-MB-231 and BT-474 breast cancer cells in a dose- and time-dependent manner This growth inhibition could be further...

Ngày tải lên: 10/09/2015, 09:09

... the conclusion that over-production of lactic acid is detrimental for tumor development and that NHE1, by controlling pHi, plays a key role in cell survival /proliferation and tumor growth Paclitaxel, ... in transformed cells Apart from its role as a principal regulator of pHi and cell volume, NHE1 has been implicated in cell proliferation and transformation, thereby linking alkaline intracellular ... important segment for NHE1’s affinity for Na+ and its sensitivity to NHE1 inhibitors TM VI and VII are important for activity, and TM XI is essential in targeting NHE1 to the cell membrane NHE1...

Ngày tải lên: 16/09/2015, 08:31

... the purinic nucleotides useful for DNA and RNA synthesis, and inosine that will be degraded into hypoxanthine and xanthine and finally into uric acid Hypoxanthine and guanine may enter in a salvage ... hyperuricemia, high tumor burden, high growth rate of tumor, high sensitivity to chemotherapy and renal impairment Standard measures to prevent and treat hyperuricemia include allopurinol and alkalinization, ... also in comparison with hydration and alkalinization, that are the standard proceedings of TLS management Hydration, that should be started before and continued for several days after the end of...

Ngày tải lên: 31/10/2012, 14:59