substrates scaffolds for neural cell growth

... the cell, cell activities, cell source, cell interaction and cell morphology Generally the cells sources can be categorized into primary cells, cell lines and stem cells 2.2.3.1 Primary cells ... 2.2.4 Cells seeding and cell behaviors The methods of cell seeding and cell behaviors play an important role in tissue engineering Cell behavior includes cell adhesion, cell spreading, cell migration ... with cell spreading The factors involved in cell spreading are distinguishable from cell adhesion Cell spreading will promote higher shape factor compare with the cell adhesion Moreover, cell...

Ngày tải lên: 22/10/2015, 21:14

... dimensional scaffolds as structural templates for cell adhesion and subsequent tissue formation, since 3D scaffolds have more available surface area for cell attachment and adequate porosity for nutrients ... passages Therefore, three-dimensional scaffolds are used to promote cell development, including appropriate cell- cell or cell- matrix interactions, normal cell functions and stability of the cells In ... engineered tissue but also biological substitutes Therefore, the factors required for tissue engineering include cells and artificial scaffolds for cell growth [Lewandrowski, 2002] Tissue engineering...

Ngày tải lên: 06/10/2015, 20:43

... viable cell counts were performed using trypan blue The proliferation status of cells was also examined by immunocytochemistry for the proliferation-associated Ki-67 antigen, as described [24] Cell ... factors for the following parameters: (a) the harvested cell number, (b) the proportion of Ki-67-immunopositive cells, (c) cell viability, and (d) the proportion of SA-βgal-positive cells All ... fit the general observation of various cell types that serum withdrawal induces cell growth arrest and/or apoptosis [27,28] whereas cell adhesion promotes cell survival [29] We also found that...

Ngày tải lên: 09/08/2014, 10:23

... crucial for GST-B4 binding, whereas the C-terminal domain is dispensable for the interaction Vpr mutants defective for GST-B4 binding are unable to arrest yeast cell growth We next tested the growth ... GST-B4 suppression of Vpr-induced cell growth arrest Yeast co-transformants were grown in non-inducible selective medium for two days Similar number of yeast cells were then serially diluted, ... cerevisiae and rescues cell growth GST-B4 peptide binds to HIV-1 Vpr in S cerevisiae and rescues cell growth (A) GST pull-down from yeast extracts S cerevisiae HP16 strain co-transformed with GST or...

Ngày tải lên: 13/08/2014, 13:20

... as cell culture scaffolds that can promote cell- substrate interactions and are bioactive in soliciting favorable cellular responses like cell adhesion, cell morphological reorganization, cell ... stromal cells provide direct cell- cell interactions as well as cell- matrix interactions through secreting ECM The stromal cells also secrete numerous different growth factors necessary for the ... a niche for cells to proliferate and differentiate The intended uses for scaffold-based cell cultures are vast: In some applications, the cells develop into tissues that are suitable for implantation,...

Ngày tải lên: 12/09/2015, 09:08

... MICROMACHINED SCAFFOLDS FOR CELL BEHAVIOUR STUDIES ZHANG FANG (B.Sc.) A THESIS SUBMITTED FOR THE DEGREE OF MASTER OF SCIENCE DEPARTMENT OF PHYSICS NATIONAL UNIVERSITY OF SINGAPORE 2004 for mum, dad ... engineering, 3D scaffolds provide the critical function of acting as extracellular matrices onto which cells can attach, grow, and form new tissue The main focus of this thesis is to understand cell behavior ... the vascular system, for growth and development [3] Angiogenesis is defined as the formation of new capillary branches from pre-existing blood vessels Endothelial cells (ECs) form the inner surface...

Ngày tải lên: 06/10/2015, 20:42

... assemble to a specified form for tissue formation To this end, cells would bind each other through the cell cell interactions and, in addition, cells attach to a substrate for their survival, proliferation, ... barrier for cells to retain the site for tissue regeneration, the guide for cells to create a tissue giving the contour of the regenerated tissue, the carrier for the sustained release of growth ... 14.4 Scaffold type II for progenitor cells Endothelial cell Smooth muscle cell Fibroblast Regenerated tissue 14.3 Minimum Requirements for Tissue Engineering Figure 14.5 Cells assembling during...

Ngày tải lên: 25/10/2013, 08:20

... sickle cell center for exams, ask for more information Help Your Child Take Care Of Himself Children with sickle cell disease need to learn to things for themselves Sometimes, it is hard for parents ... Chapter ♦ KEY GUIDELINES FOR PARENTS K ey Guidelines for Parents Most children with sickle cell disease can look forward to a full life It is important that they be prepared for adulthood and living ... Chapter ♦ KEY GUIDELINES FOR PARENTS Key Guidelines for Parents See Appendices A and B for answers to the questions “What is Sickle Cell Disease?” and “What Causes Sickle Cell Disease?” Prevent...

Ngày tải lên: 12/02/2014, 12:20

... intracellular localization of ER and PDIP46 ⁄ SKAR fused to EGFP in the mammalian cells NIH ⁄ 3T3 cells (upper) and HeLa cells (lower) were transiently transfected with plasmids coding for ER-EGFP ... fluorescence detection in living cells was performed The revised intracellular localization of ER is instrumental in validating the results of the performed screen Both isoforms of PDIP46 ⁄ SKAR are ... regulates the cell growth [23,24] A bipartite region of PDIP46 ⁄ SKAR necessary for interaction with ER encompassing the S6K1 binding and phosphorylation sites was mapped In addition, the intracellular...

Ngày tải lên: 19/02/2014, 05:20

... concentrations of choline abolishes daughter cell separation at the end of cell division, causing the formation of long chains of cells with only small effects on cell growth and viability, even at high ... Instead of forming long chains of cells without the growth rate being affected, atropine-treated or ipratropium-treated cultures showed retardation or complete cessation of growth, clear cell deformation, ... pneumococcal cell wall lytic enzymes Purified choline-binding enzymes LytA, LytC and Pce were used for cell wall degradation assays, performed basically as previously described [8], using pneumococcal cell...

Ngày tải lên: 19/02/2014, 05:20

... resulted in cell growth arrest, which showed dosedependent and time-dependent effects In addition, colony formation is typical of malignant transformed cells, and is inconspicuous in normal cells Using ... Overexpression of miR-9 suppresses cell growth in vitro (A) Cell growth was measured using the 3-(4,5-dimethylthiazol-2yl)-2,5-diphenyl-tetrazolium bromide (MTT) assay ES-2 cells were transfected with ... used to determine relative cell growth activity at 24, 48 and 72 h (D) The effect of miR-9 on cell proliferation was evaluated using a colony formation assay ES-2 cells were transfected with...

Ngày tải lên: 07/03/2014, 00:20

... immunofluorescence A a b c B Fig PGs on HeLa cells (A) Cell surface expression of SD-1, SD-4 and CD44 on HeLa cells HeLa cells (5 · 105) were stained for FACS analysis with anti-(SD-1) DL-101 mAb ... not involved in intracellular Ca2+ mobilization induced by SDF1 on HeLa cells Untreated HeLa cells (A), heparitinases I- and III-treated HeLa cells (B), mock-transfected HeLa cells (C), or SD-4 ... SDF-1 stimulation of the cells (Fig 4A, lane vs 1, and lane vs 3) These increases were not significant if the cells were stimulated with nm SDF-1, and strongly significant for a cell stimulation with...

Ngày tải lên: 16/03/2014, 18:20

... cells after forskolin treatment for 24 h [18] Here, we determined the levels of expression of CD98 by western blotting in cell extracts from BeWo cells cultured with or without forskolin for 12 h, ... BeWo cells at 50–60% confluence were incubated in medium containing dimethylsulfoxide (vehicle control) or 100 lM forskolin (Forsk) for 24 h at 37 °C BFA was added for the indicated times before ... acid transport and cell fusion in BeWo cells [15–17] More recently, we have shown that CD98 involvement in the process of cell fusion that is necessary for syncytiotrophoblast formation is a distinct...

Ngày tải lên: 23/03/2014, 09:20

... components: the growth of the capital input, growth of the labor input, and the (unexplained) growth in productivity of all factors (TFP) The production function, which forms the basis of growth accounting ... attracting foreign investment Asian data provide some support for the hypotheses that gender wage inequality is a stimulus to growth and investment Figure shows the average annual rate of GDP growth for ... relationship between gender equity and growth, we need to control for additional factors that may be influencing the rate of growth A growthaccounting approach is used for consistency with the methodology...

Ngày tải lên: 29/03/2014, 20:20

... lectin ligand cell surface binding was performed with the following human tumor lines: Croco II (B-lymphoblastoid cell line), CCRF-CEM (T-lymphoblastoid cell line), K-562 (erythroleukemia cell line), ... leukemia cell line), DU4475 (mammary carcinoma cell line) as well as C205, SW480 and SW620 (colon adenocarcinoma cell lines) Except for the Croco II line established in our laboratory [56] the cells ... encounters a cell surface, the neoglycoprotein A reacted with cell surfaces in a cell type-specific manner (Fig 4) As highlighted by these results, distinct preference of binding was determined for the...

Ngày tải lên: 30/03/2014, 16:20

... Carbohydrate Analysis (2nd edition) Cell- Cell Interactions The Cell Cycle Cell Growth and Apoptosis if Cell Separation Cellular Calcium Cellular Interactions in Development Cellular Neurobiology * Chrornatin ... Ki67, and AgNOR References Cell growth and cytotoxicity assays Philip Skehan Introduction Growth and cytotoxicity assays Quantifying cell growth Experimental design Growth rate and doubling time ... cells Long-term recovery (LTR) assay Colony-forming efficiency on tissue culture plastic Colony-forming efficiency in soft agar 53 54 54 56 57 References 59 Cell growth and kinetics in multicell...

Ngày tải lên: 10/04/2014, 11:13

... at 95°C for min, and then 30 cycles at 94°C for 45 s, 54°C for min, 72°C for and a final extension cycle of 72°C for The house-keeping gene b-actin was taken as a loading control HEK293T cells ... based on cell percent with GFP expressing The number of GFP-expressing cells versus the total cell quantity in the microscope was defined as the transfection efficiency Cell counting was performed ... typically, several major cell types are used in vitro for testing including phagocytic, epithelial, endothelial and various cancer cell lines [20] Therefore, besides the renal cancer cell OS-RC-2, we...

Ngày tải lên: 18/06/2014, 19:20

... at 95°C for min, and then 30 cycles at 94°C for 45 s, 54°C for min, 72°C for and a final extension cycle of 72°C for The house-keeping gene b-actin was taken as a loading control HEK293T cells ... based on cell percent with GFP expressing The number of GFP-expressing cells versus the total cell quantity in the microscope was defined as the transfection efficiency Cell counting was performed ... typically, several major cell types are used in vitro for testing including phagocytic, epithelial, endothelial and various cancer cell lines [20] Therefore, besides the renal cancer cell OS-RC-2, we...

Ngày tải lên: 20/06/2014, 03:20

... as nucleation sites for the selective area growth of GaN NCs Once the process window for the ordered growth of GaN NCs by PA-MBE was identified, the successful selective growth was driven by ... at 10−2 mbar and 110 W (Plasmalab, Oxford Instruments plc, Abingdon, Oxfordshire, UK) for 130 s The post-etch residue was removed by immersing in NMP at 80°C for 30 and abundantly rinsing in IPA ... the growth stage The Ga and N fluxes were calibrated in equivalent (0001) GaN growth rate units for compact layers in nanometers per minute, which are the standard units used in nitride PA-MBE growth...

Ngày tải lên: 20/06/2014, 04:20

... http://www.ovarianresearch.com/content/3/1/11 microscope cell images were taken on an Olympus BX 41 microscope (Olympus, Center Valley, PA) Colony formation assay For this assay, cells were seeded at 500 cells per 100 mm culture ... A2780CP cells (Figure 1B, CUR + CIS) However, pre-treatment with 20 μM curcumin for hrs followed by treatment with 2.5-40 μM cisplatin for an additional 42 hrs resulted in drastic cell growth ... effectively inhibits the growth of cisplatin resistant ovarian cancer cells A2780CP cells were treated with Nano-CUR (5-80 μM) or PLGA NPs without curcumin (NPs control) for 48 hrs Cell proliferation...

Ngày tải lên: 20/06/2014, 07:20