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Tài liệu Báo cáo khóa học: Quantitative analysis, using MALDI-TOF mass spectrometry, of the N-terminal hydrolysis and cyclization reactions of the activation 2 process of onconase pdf
Ngày tải lên :
19/02/2014, 12:20
... simplify the protocol and shorten
the time needed to obtain a fully active ONC compared with
other procedures described in the literature. The application
of MALDI-TOF MS to the characterization of ... analyzed by MALDI-TOF MS. The
addition of the protease is considered the zero time point.
The relative intensities of the peaks from the mass spectra
were used to calculate the fraction of Pyr-ONC (M23L), ... the conversion of glutaminyl to pyroglutamyl
residues.
The hydrolysis reaction was monitored by calculating the
sum of the relative intensities of the peaks corresponding to
Gln-ONC (M23L) (theoretical...
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Tài liệu Euphorion Being Studies of the Antique and the Mediaeval in the Renaissance - Vol. II pot
Ngày tải lên :
21/02/2014, 21:20
... hills to the north, into the widening torrent valleys of the Modenese Apennines to the south, the
Marquisate of Este stretches up into feudalism, into chivalry, into the imaginative kingdom of the ... workmen to heave the great beams, to carry the great stones
of the cathedral, to split the huge marble masses while they stared in astonished envy; as he sat, unconscious
of their mutterings, ... Boiardo, of the Ferrarese court to which he belonged, and of the school of
poetry (including the more notable but less original work of Ariosto) which the genius of the man and the
character of the...
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Báo cáo Y học: Identification of residues critical for activity of the wound-induced leucine aminopeptidase (LAP-A) of tomato pptx
Ngày tải lên :
08/03/2014, 23:20
... and
stabilization of the ge m-diolate transition state. The Ala
substitution mutant (K354A) of the tomato His
6
–LAP-A
was not evaluated in the tomato LAP-A1 series of
mutations.
The tomato residues ... studies of the bovine
lens LAP a nd E. coli PepA provided a solid foundation for
the study into the residues critical of the activity of the
wound-induced tomato LAP-A1 [6,22,24]. Similar to the
animal ... identified the
residues important for LAP catalysis and coordination of
the two zinc io ns in the a ctive site. The i mpact of the
mutations on the tomato LAP enzyme must be viewed in
the context o f these...
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Structure of the S15,S6,S18-rRNA Complex:Assembly of the 30S Ribosome Central Domain
Ngày tải lên :
13/03/2014, 19:08
... fold, while the S15 contacts
above the internal loop of helix 22 and to the
tetraloop of helix 23a stabilize the tertiary fold
of the upper three-helix junction. The tertiary
structure of the upper ... contacts to the lower three-helix
junction, to the minor groove of helix 22 above
the purine-rich internal loop, and to the GAAG
tetraloop in helix 23a (Figs. 2C and 4). The S15
contacts to the lower ... reections. The phasing power is the ratio of
the mean calculated heavy-atom structure factor amplitude to the mean lack of closure error. #R
cryst
ϭ⌺F
o
͉ Ϫ ͉F
c
/⌺ ͉F
o
͉.
Fig. 1. Assembly of the
16S...
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Báo cáo khoa học: The propeptide of cruzipain ) a potent selective inhibitor of the trypanosomal enzymes cruzipain and brucipain, and of the human enzyme cathepsin F ppt
Ngày tải lên :
16/03/2014, 11:20
... due to the
occluding loop in the primed side of the binding site
groove. Although the prodomains of cathepsins F and
W share the same degree of similarity to the prodo-
main of cruzipain, the ... aimed at the inacti-
vation of CPs from several pathogenic protozoa.
The propeptide of cruzipain is a potent inhibitor
of cathepsin F
To evaluate the selectivity of PCZ as an inhibitor of
papain-like ... RGVDLQPS-
LIS at the C-terminus, which resulted from the fusion of
the propeptide with a small stretch of coding sequence
within the multiple cloning site of the pQE30 plasmid
before the stop codon. The predicted...
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Báo cáo Y học: Amino acids 3–13 and amino acids in and flanking the 23FxxLF27 motif modulate the interaction between the N-terminal and ligand-binding domain of the androgen receptor pdf
Ngày tải lên :
17/03/2014, 10:20
... a-helix;
FxxLF.
The androgen receptor (AR) is a member of the steroid
receptor subgroup of the nuclear receptor family of
transcription factors. Nuclear receptors have a modular
structure, composed of a ... inhibits the interaction
with coactivators and enables the binding of corepressors
[3,5].
P160 coactivators not only bind to the LBD, but also to
the NTD [6,7]. This interaction is independent of the ... a
specific role of these motifs in AR function [44–47]. The
increasing number of proteins found to interact with the AR
LBD raises the question of the physiological relevance of the
many interactions....
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Báo cáo khoa học: Determination of the redox potentials and electron transfer properties of the FAD- and FMN-binding domains of the human oxidoreductase NR1 doc
Ngày tải lên :
23/03/2014, 21:20
... Aggregation of the NR1
FAD/NADPH domain was much less extensive in the
absence of cofactor reduction.
Stopped-flow kinetic studies
Reduction of the FAD/NADPH domain of NR1 was
investigated by stopped-flow ... obligatory
2-electron donor. These electrons are then transferred in a
finely coupled stepwise manner to various physiological
redox acceptor proteins, in the case of CPR to the P450
enzymes bound to the endoplasmic ... domain. The presence of the His-
tag on the FAD/NADPH domain had no apparent effect
on catalytic activity.
The purified domains were yellow, indicating the presence
of bound cofactor, and they displayed...
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Báo cáo khoa học: Internalization of the human CRF receptor 1 is independent of classical phosphorylation sites and of b-arrestin 1 recruitment potx
Ngày tải lên :
30/03/2014, 15:20
... for
several GPCRs [29]. The function of b-arrestin is to uncouple
the receptor f rom the G-protein, thereby mediating recepto r
desensitization, and furthermore to couple the receptor to
the endocytic ... the shape of the cell following agonist stimulation
is observed with the Substance P receptor [25]. Detachment
of the cells from the surface of the culture dish could r eflect
disassembly of the ... receptor.
However these mutations abolished the profound recruit-
ment of b-arrestin 1 to the plasma membrane observed in
response to stimulation of the wild-type receptor, indicating
that receptor...
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Báo cáo khoa học: Expression, purification and characterization of the second Kunitz-type protease inhibitor domain of the human WFIKKN protein pot
Ngày tải lên :
31/03/2014, 01:20
... raising the
possibility that the inability of WFIKKN-KU2 to inhibit
these proteases is partly due to the lack of such a favorable
interaction of the PÂ2 Trp with the target enzymes. The fact
that the ... dissociation constant of the trypsin–inhibitor com-
plex, K
i
was determined from the replot of the apparent K
m
values vs. the inhibitor concentration at which they were
obtained.
In the case of chymotrypsin, ... [14–19]. In view of the
fact that the structure of the Kunitz inhibitor of the sea
anemone Stichodactyla helianthus is nearly identical with
that of the bovine pancreatic trypsin inhibitor despite...
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Báo cáo hóa học: " Comparison of the self-administered and interviewer-administered modes of the child-OIDP" ppt
Ngày tải lên :
18/06/2014, 22:20
...
Abstract
Background: The mode of questionnaire administration may affect the estimates and applicability
of oral health-related quality of life indicators. The aim of this study was to compare
psychometrically the ... Child-OIDP. The self-administered ver-
sion of the Child-OIDP performed similarly to the inter-
viewer-administered original version of the instrument.
Despite the demonstrated appropriateness of the ... braces. In terms of internal consistency,
there was no significant difference between the FTFI and
the SAQ groups in relation to any of the 8 item-total cor-
relations as well as the overall alpha,...
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báo cáo hóa học:" The occurrence of osteoarthritis at a minimum of ten years after reconstruction of the anterior cruciate ligament" pdf
Ngày tải lên :
20/06/2014, 01:20
... per-
formance, the score may appear too high, and will reflect
the actual function of the knee as well as the satisfaction
of the patient. The Lysholm score has never been validated
for the purpose of following ... progression rate of OA [21,54-57]. Yet,
several of these reports fail to present adequate data on the
age of the patients at time of the injury. In this study a sub-
group analysis to evaluate differences ... grade 2 to 3 and positive Pivot-shift test). These
patients were considered at high risk of further knee injury
due to the degree of laxity and the desired level of activity
[27]. In order to minimise...
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báo cáo hóa học:" Revision hip replacement for recurrent Hydatid disease of the pelvis: a case report and review of the literature" doc
Ngày tải lên :
20/06/2014, 04:20
... After
ingestion by the host, the embryos migrate through the
intes tinal wall and are either arrested in the capill ary bed
of the liver developing into liver cysts, or manage to
penetrate into systemic ... in
remote organs. Due to their physiologic role as capillary
filters and their vast capillary volume, the liver and lung
are most often affected. The brain, the muscle s or the
bones are the more frequently ... recur-
renceofthecystsdespitetheongoingAlbendazole
treatment. She returned again on 8/7/08, with increasing
pain in the right hip. X-ray has shown that loosening of
the acetabular component of the total...
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báo cáo hóa học:" Repositioning and stabilization of the radial styloid process in comminuted fractures of the distal radius using a single approach: the radio-volar double plating technique" pot
Ngày tải lên :
20/06/2014, 04:20
... in the interval between
the flexor carpi radialis tendon and the radial artery.
The distal part of the pronator quadratus muscle was
released from the radius. Care was taken to release only
the ... drafting of the manuscript.
GK participated in the design of the study and coordination and helped to
draft the manuscript. All authors read and approved the final manuscript.
Competing interests
There ... possible difficulty in intra-articular fracture of the distal radius is the displacement tendency of the
radial styloid process due to the tension of the brachioradialis tendon.
Methods: Ten patients...
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Báo cáo hóa học: " Impact of the environment and the topology on the performance of hierarchical body area networks" ppt
Ngày tải lên :
20/06/2014, 22:20
... interval equal to the duration of a
packet transmission). Therefore, the network load G
(adimensional) is simply equal to q · N
tot
,whereN
tot
denotes the total number of sensor nodes in the BAN.
Let
N
be ... [24], the adaptation of the sampling to
rate to patient’s condition [25], the MAC strategy used
[26], etc. Since the impact of these parameters is not the
focus of the this study, the interested reader ... leaves is higher than the maximum
throughput at the relays. As the number of relays is
relatively large, they tend to interfere with each other
and, therefore, the throughput of the sink re aches...
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