dough strengthening effects of emulsifiers

... interfacial material can be composed of proteins or of small emulsifiers such as monoglycerides, esters of fatty acids, or phospholipids, or mixtures of a number of these components Finally, the ... approximately 11 nm2 of interface [calculated on the basis of a molecule of 20,000 Da and a surface coverage of mg/m2; this agrees well with the expected dimensions of a globular protein of this weight ... simplest of these methods depends on the measurement of turbidity at one or a number of wavelengths (107,108) Turbidity, or apparent absorbance of light, is a measure of the total amount of light...

Ngày tải lên: 23/05/2014, 11:36

... TABLE OF CONTENTS II LIST OF FIGURES IV LIST OF TABLES VII LIST OF PUBLICATIONS VIII LIST OF ABBREVIATIONS IX SUMMARY XI INTRODUCTION 1.1 Medical Importance of HCV 1.2 Molecular biology of HCV ... PURIFICATION OF NS3 HELICASE BY FPLC 33 FIGURE 3-7 GEL FILTRATION OF WILD-TYPE HELICASE 34 FIGURE 3-8 GEL FILTRATION OF HELICASE MUTANTS 36 FIGURE 3-9 POSITIONS OF SOME OF THE MUTANTS ... region of interaction between NS3 and LMP7 3.2.3 Expression and purification of recombinant NS3 and LMP7 for in vitro assays 3.2.4 Effect of LMP7 on NS3 protease activity 3.2.5 Effect of NS3...

Ngày tải lên: 16/09/2015, 17:12

... concentrations of NaCl, the fluor3888 escence intensities of GST–HIPPI increased, indicating a lesser extent of interactions of GST–HIPPI with AA AGACATG This result indicated that the interaction of GST–HIPPI ... Quenching of intrinsic fluorescence of GST–HIPPI (0.8 lM) at 340 nm (kexc ¼ 295 nm) in the presence of the bp putative motif sequence and six of its mutants Panel I Fluorescence quenching of GST–HIPPI ... in a Fluoromax spectrofluoremeter Panel II Study of any quenching of intrinsic fluorescence of GST– HIPPI due to addition of point-mutated sequences of the bp potent HIPPI-binding motif, namely:...

Ngày tải lên: 07/03/2014, 10:20

... derived from acrylamide titration of a vesicle blank Ksv, which is a measure of the accessibility of Trp to acrylamide, was obtained from the slope of the plots of F0 ⁄ F vs [quencher], where F0 ... quenchers Assay of calcein leakage Dequenching of encapsulated calcein fluorescence resulting from the leakage of aqueous content out of LUVs was used to assess the vesicle leakage activity of the peptides ... virus to HCV, both of them belonging to the small enveloped viruses of the Flaviviridae family A stretch of conserved, hydrophobic amino acids within the E2 envelope glycoprotein of HCV has been...

Ngày tải lên: 07/03/2014, 17:20

... a truncation of half of its F3 subdomain We used the same experimental conditions as in Fig 3A Deletion of the C-terminus of Krit1 abolished the binding ´ S Beraud-Dufour et al of Rap1GTPcS to ... generated Mutagenesis analyses of these residues will help to determine whether the binding site of the polar head of PIP2 on Krit1 is similar to that of radixin Many members of the FERM family undergo ... binding Rap1 GTPcS on lm of GST-HypoKrit1 beads in absence or presence of 10 lm of Krit1-NTer Importantly, Krit-NTer binding to GST-HypoKrit1 did not affect the interaction of Rap1GTPcS to GST-HypoKrit1...

Ngày tải lên: 16/03/2014, 05:20

... use of their equipment and to the University of Edinburgh and BBSRC for funding of this project References Reed SI (1980) The selection of S cerevisiae mutants defective in the start event of ... Overexpression of the truncation Cdc37(1–155), which lacks part of the N-terminal domain, was unable to support cellular viability A Fig Overexpression of the N-terminal domain of Cdc37 sustains ... phenotype, characteristic of depletion of Cdc37 [23] According to the alignment of p50 and S pombe Cdc37 shown in Fig 2B, the Cdc37-I252 insertion is located at the edge of the postulated Hsp90...

Ngày tải lên: 16/03/2014, 22:20

... regulation of enzyme activity of a number of proteins [23] A number of studies have suggested that interaction of m-calpain domain V with lipid or membranes may also be involved in the modulation of ... analysis of lipid in the presence of VP1 In the absence of the peptide, the phase-transition temperature (Tc) of Myr2PtdEtn was recorded as 55 °C (j; A), of Myr2PtdCho as 27 °C (j; B) and of Myr2PtdSer ... presence of 100 mM NaCl, this ability of VP1 was reduced the order of 60% (LD50 ¼ 1.85 mM) FTIR conformational analysis of VP1 FTIR spectroscopy was used for conformational analysis of VP1, either...

Ngày tải lên: 17/03/2014, 10:20

... number of cofactor-binding sites decreased in the presence of PfACP, the affinity of PfFabG for NADPH increased, indicating a negative, heterotropic cooperative effect of PfACP upon binding of NADPH ... entrance of the active site tunnel (Fig 1C) [12] The sequence alignment of FabG from different Plasmodium species, however, shows the presence of Lys187 instead of Arg187, so the effects of mutations ... estimate the association constants and number of binding sites of different mutants of PfFabG and PfACP, as well as the interactions in the presence of the cofactor NADPH Studies with E coli FabG have...

Ngày tải lên: 23/03/2014, 10:20

... production of lysophospholipids, e.g the absence of scavengers, direct oxidation or phospholipase A2 attack The mode of action of ostreolysin is probably also common to other members of this protein ... toxins Specic expression of ostreolysin and aegerolysin during initiation of fungal fruiting [4,5], expression of a hemolysin-like protein of B bifermentans at the stage of sporulation [7], and ... eects of osmotic protectants Traces were obtained in the presence of 30 mM glucose, sucrose or poly(ethylene glycol)s of dierent average molecular masses as indicated (Fig 1C) The inner diameter of...

Ngày tải lên: 23/03/2014, 21:20

... elution profiles of the two C-domain deletion mutants were compared, the peak of DC1 was obviously broader than that of DC2 In some previous reports, the C2 domain of MFG-E8 as well as that of blood ... lm in diameter, and 80% or more of the total number of globules are below lm Formation of the complex of butyrophilin, xanthine oxidase and surface molecules of cytoplasmic lipid droplets is ... medium The expression of several domain-deletion mutants of MFG-E8 suggests contribution of the C2 domain to the association with PtdSer and plasma membrane and subcontribution of the C1 domain to...

Ngày tải lên: 24/03/2014, 03:21

... concentration of 25 lM The fluorescence was recorded as a function of time using an excitation wavelength of 360 nm and an emission wavelength of 530 nm with nm bandwidth slits A 490-nm cutoff filter ... This is opposite to the direction of the shift of TH of +1800 ± 300 °CÆmol)1 found with the addition of magainin II [29] and does not correspond to the type of curvature required for a large ... in the cell and incremental addition of vesicles The results of titration of LUVs of dielaidoylphosphatidylethanolamine/ Ole2PtdGro (1 : 2) into a solution of b-17 is given in Fig 8A Similar titrations...

Ngày tải lên: 31/03/2014, 07:20

... role in the treatment of diseases were selected and compared for their binding affinities towards the design of a new series of drugs In order to find the new series of ligands of the above proteins, ... 4.20 RMSF curve of PPAR-γ with DHA 149 15 Figure 4.21 Comparison of Binding Energies of PPAR-α in AutoDock and Glide 154 Figure 4.22 Comparison of Binding Energies of PPAR δ in AutoDock ... Comparison of Binding Energies of PPAR-γ in AutoDock and Glide 155 Figure 4.24 Binding of RAR-γ with DHA 160 Figure 4.25 Binding of RXR-α with DHA 161 Figure 4.26 Binding of...

Ngày tải lên: 28/11/2015, 14:01

... The SH3 domain of Fyn binds to proline-rich motifs within the sequence PXXP in interacting proteins Seven such motifs are present in the proline-rich domain of the longest isoform of tau in the ... this band was not apparent on western blots of lysates of Y394F tau (Fig 2A) The absence of this larger species could indicate that phosphorylation of tau by Fyn is reduced and therefore, in addition ... tau revealed decreased electrophoretic mobility of tau, with the appearance of an  68-kDa tau species in wild-type and all of the mutant forms of tau except for YallF and Y394F tau (Fig 3) In...

Ngày tải lên: 14/02/2014, 14:20

... temperature or the speed of synthesis and decay of the RNA molecule [15,16], are major determinants of the folding kinetics Further factors that affect the speed and reaction route of RNA folding are ... to millimolar range, because the off-rates are high (koff ‡ 0.2 s)1) [25] A further way of describing macromolecular complexes is by the molecular interface of the interacting molecules In common ... the mechanism of acceleration of annealing [65] We found that Tat(44–61) efficiently annealed both short RNA and DNA substrates of different length and sequence The annealing activity of the peptide...

Ngày tải lên: 14/02/2014, 19:20

... probably because of the transient nature of the interaction in solution (Fig 1) The recent assignment of the NMR spectrum of the PCNA ring [6,7] provides the basis for solution studies of its interactions ... systems [12], allowing the study of the kinetics of translocation [13,14], as well as the structures of tran1644 Fig Structure of a PCNAÆDNA complex Two views of the homotrimeric yeast PCNA ring ... histone H4 tail of a neighbor nucleosome in the crystal lattice of the nucleosome [34] Fig Structure of the nucleosome with bound RCC1 proteins Ribbon diagram of the structure of the RCC1Ænucleosome...

Ngày tải lên: 14/02/2014, 19:20

... Drug Application ORA Office of Regulatory Affairs ORO Office of Regional Operations OSD Office of the Secretary of Defense PB pyridostigmine bromide PEO Program Executive Officer PHSA Public Health ... review of information provided to individual recipients of IND drugs, medical monitoring and reporting of the use of such drugs, information about shipment and storage of drugs, disposition of unused ... represented by the Office of the Assistant to the Secretary of Defense for Nuclear, Chemical, and Biological Warfare; the The Challenges of Acquisition 27 DATSD(CBD); the ASD(HA); the Office of the General...

Ngày tải lên: 17/02/2014, 11:20

... Fluorescence titration of the FMN domain with the hMS AD Fig S2 Comparison of the electrostatic potentials of the surface of the CPR FMN domain and of a model of the FMN domain of human MSR Fig S3 ... addition of homocysteine following of incubation at 37 °C of the enzyme with all other components Following 10 of incubation at 37 °C, the reaction was quenched by the addition of 0.2 mL of a solution ... diflavin reductases and also subdomains of MSR; (b) the extent of reductive remethylation of hMS catalysed by the different redox states of MSR; and (c) the ability of structurally related diflavin reducatases...

Ngày tải lên: 18/02/2014, 08:20

... there was a band of a protein with electrophoretic mobility of approximately 46 kDa, which corresponds to the molecular mass of isoform ⁄ a of PDIP46 ⁄ SKAR (Fig 3C) The band of this protein was ... plasmids using Lipofectamine Reagent according to the manufacturer’s suggested protocol (Invitrogen) Briefly, a mixture of lg of the plasmid DNA and lL of Lipofectamine in mL of antibiotic- and ... found to consist of 666 bp containing the 3¢ end of the POLDIP3 coding sequence (with the stop codon) and coded for 163 amino acids present at the C-terminus of both isoforms of PDIP46 ⁄ SKAR,...

Ngày tải lên: 19/02/2014, 05:20

... observed, confirming the nature of the cofactor [19] By using the extinction coefficient at 450 nm we calculated that the stoichiometric ratio of flavinic cofactor per mole of protein reaches 1.02 mol, ... Interaction of AtuNDH-2 with membranes of the E coli mutant ANN0222 and effect of DPI (A) Effect of AtuNDH-2 on NADH-dependent O2 uptake measured in bacterial membranes following addition of 200 lM ... (Sigma-Aldrich) The composition of the mobile phase (flow rate of mLÆmin)1) was 80% of 0.1% TFA in water and 20% of 0.1% TFA in 40% acetonitrile Excitation and emission wavelengths of the fluorescence detector...

Ngày tải lên: 19/02/2014, 06:20