dopaminergic neuronal differentiation protocol for human mesenchymal stem cells

... 43 2.3 Stem cells 51 2.3.1 Definition and classification 51 2.3.2 Stem cells in tissue engineering 53 2.3.3 Mesenchymal stem cells 54 v 2.4 Stem cell ... ACID)-BASED NANOFIBERS FOR OSTEOGENIC DIFFERENTIATION OF HUMAN MESENCHYMAL STEM CELLS NGUYEN THI HIEN LUONG (B.Eng., Ho Chi Minh city University of Technology, Vietnam) A THESIS SUBMITTED FOR THE DEGREE ... would effectively induce osteogenic differentiation of stem cells without the need for osteogenic solutes The scaffolds were enriched with mesenchymal stem cells (MSCs) to provide the osteogenic...

Ngày tải lên: 09/09/2015, 10:07

... of cells was determined spectrophotometrically entiation of human mesenchymal transcripts Expression of BMP-2 and chordinstem cells during osteogenic differentiation of human mesenchymal stem cells ... osteogenic differentiation in human MSCs (a) Alkaline phosphatase (ALP) activity at MSCs day 10 (b) Calcium deposit at day 21 (c) Alizarin Red staining after 21 days in culture Human mesenchymal stem cells ... used were from Sigma-Aldrich (St Louis, MO, USA), unless stated otherwise Culture of human mesenchymal stem cells Human MSCs were obtained from two sources, the first one being a commercially available...

Ngày tải lên: 09/08/2014, 10:23

... differentiation of cultured human mesenchymal stem cells from marrow Tissue Eng 1998, 4:415-428 35 Mueller MB, Tuan RS: Functional characterization of hypertrophy in chondrogenesis of human mesenchymal ... with human mesenchymal stem cells Tissue Eng 2002, 8:131-144 24 Johnstone B, Hering TM, Caplan AI, Goldberg VM, Yoo JU: In vitro chondrogenesis of bone marrow-derived mesenchymal progenitor cells ... viral gene transfer of transforming growth factor-beta1 to human mesenchymal stem cells improves cartilage repair Gene Ther 2007, 14:804-813 Page 14 of 15 (page number not for citation purposes) 13...

Ngày tải lên: 09/08/2014, 14:22

... in vitro Stem Cells 2005, 23(4):594-603 Segev H, Fishman B, Ziskind A, Shulman M, Itskovitz-Eldor J: Differentiation of human embryonic stem cells into insulin-producing clusters Stem Cells 2004, ... Kao LS, Ma HL: In vitro differentiation of size-sieved stem cells into electrically active neural cells Stem Cells 2002, 20(6):522-529 Tzeng SF, Tsai MJ, Hung SC, Cheng H: Neuronal morphological ... promote differentiation of human mesenchymal stem cells into insulin producing cells J Biomed Mater Res A 2008, 86(4):1097-1105 Chao KC, Chao KF, Fu YS, Liu SH: Islet-like clusters derived from mesenchymal...

Ngày tải lên: 10/08/2014, 05:21

... threedimensional human mesenchymal stem cell osteogenic differentiation Biomaterials (1):66-77 71 Serakinci N, Guldberg P, Burns JS, et al 2004;Adult human mesenchymal stem cell as a target for neoplastic ... osteogenic differentiation of human mesenchymal stem cells Exp Cell Res (1):22-37 101 Salasznyk RM, Klees RF, Boskey A, et al 2007;Activation of FAK is necessary for the osteogenic differentiation of human ... human mesenchymal stem cells from marrow Tissue Eng (4):415-428 129 Beresford JN, Graves SE, Smoothy CA 1993;Formation of mineralized nodules by bone derived cells in vitro: a model of bone formation?...

Ngày tải lên: 11/09/2015, 09:16

... 23 24 25 26 Multilineage potential of adult human mesenchymal stem cells Science 1999, 284:143-147 Prockop DJ: Marrow stromal cells as stem cells for nonhematopoietic tissues Science 1997, 276:71-74 ... A, Sekiya I: Increased proliferation of human synovial mesenchymal stem cells with autologous human serum: comparisons with bone marrow mesenchymal stem cells and with fetal bovine serum Arthritis ... after filling human synovial MSCs in Page of 10 (page number not for citation purposes) to vivo analysis of defect Ex human cartilage human synovial mesenchymal stem cell attachment to human cartilage...

Ngày tải lên: 09/08/2014, 10:23

... cell-mediated mesenchymal stem cell differentiation Stem Cells 2006, 24:707-716 Le Visage C, Kim SW, Tateno K, Sieber AN, Kostuik JP, Leong KW: Interaction of human mesenchymal stem cells with disc cells: ... intervertebral disc-like cells from adult mesenchymal stem cells Stem Cells 2005, 23:403-411 11 Yoshikawa T, Ueda Y, Miyazaki K, Koizumi M, Takakura Y: Disc regeneration therapy using marrow mesenchymal cell ... Multilineage potential of adult human mesenchymal stem cells Science 1999, 284:143-147 Wuertz K, Godburn K, Neidlinger-Wilke C, Urban J, Iatridis JC: Behavior of mesenchymal stem cells in the chemical...

Ngày tải lên: 12/08/2014, 15:23

... Osteogenic Cells 46 2.8.2.2 Mesenchymal Stem Cells 48 2.8.2.3 Mesenchymal Stem Cells in Bone Tissue Engineering .49 2.8.2.4 Proposed Cell Type – Human Fetal Mesenchymal Stem Cells ... Analysis 87 Chapter – Coculture of Human Umbilical Cord Blood Endothelial Colony-Forming Cells with Human Fetal Mesenchymal Stem Cells for the Generation of Vascularised BTE Grafts ... Chong, Z Zhang, MA Choolani, SH Teoh Coculture of human endothelial progenitor stem cells and bone marrow-derived human fetal mesenchymal stem cells potentiates osteogenesis through paracrine...

Ngày tải lên: 09/09/2015, 10:16

... 2007) Embryonic stem cells Mesenchymal stem cells, Hematopoietic stem cells Oligopotent Can differentiate into only a few cells (Sorgner, 2007) Lymphoid stem cells, Myeloid stem cells Unipotent ... embryonic germ cells, fetal stem cells, umbilical cord stem cells, adult stem cells and induced pluripotent stem (iPS) cells (Ariff Bongso, 2005, see Table 2) Each type of stem cells holds its ... cell source for future clinical applications Literature review Table Characterization of stem cells by derivation source Category Embryonic stem cells Embryonic germ cells Fetal stem cells Umbilical...

Ngày tải lên: 11/09/2015, 10:00

Ngày tải lên: 07/08/2014, 18:21

... from human placenta Stem Cells 22, 1338–1345 Bieback K, Kern S, Kluter H & Eichler H (2004) Critical parameters for the isolation of mesenchymal stem cells from umbilical cord blood Stem Cells ... differentiation from stem cells For microarray analysis, we confirmed the hepatic differentiation of AT-MSC into hepatocyte-like cells using the original protocol (Fig 1A) The differentiated cells ... vitro differentiation of human umbilical cord blood-derived mesenchymal stem cells into hepatocytelike cells Biochem Biophys Res Commun 330, 1153–1161 Seo MJ, Suh SY, Bae YC & Jung JS (2005) Differentiation...

Ngày tải lên: 30/03/2014, 04:20

... serum free conditions for 14 days (Figure 3B) For co-cultured CD34+ hematopoietic stem cells with 3A6 cells, we noted the formation of cobblestone areas from hematopoietic cells that transmigrated ... hMSCs converges toward embryonic stem cells Principal component analysis comparing the gene expression profiles of 3A6, embryonic stem cells (ESC), mesenchymal stem cells (MSC), and various tissues ... Oct4 and Nanog is associated with differentiation of neural stem cells, hematopoietic stem cells and MSCs However, an increase in Oct4 expression in ESCs causes differentiation into primitive endoderm...

Ngày tải lên: 10/08/2014, 05:21

... and the cells were washed twice with PBS, fixed for 30 with 4% paraformaldehyde Cells were permeabilized for 20 with 0.1% Triton X-100 and then blocked for 30 in 5% normal goat serum Cells were ... Review: Human Umbilical Cord Stroma with Regard to the Source of Fetus-Derived Stem Cells Stem cells 2007, 25:2886-2895 Wu KH, Mo XM, Zhou B, Lu SH, Yang SG, Liu YL, Han ZC: Cardiac potential of stem ... stem cells from whole human umbilical cord tissue J Cellular Biochem 2009, 107:926-932 Yang C-C, Shih Y-H, Ko M-H, Hsu S-Y, Cheng H, Fu Y-S: Transplantation of Human Umbilical Mesenchymal Stem Cells...

Ngày tải lên: 10/08/2014, 05:21

... potential of amniotic fluid-derived human mesenchymal stem cells Stem Cells Dev 2006, 15:719-726 55 Montaln H, Schichor C, Lah TT: Human mesenchymal stem cells and their use in cell-based terapies ... Chamberlain G, Fox J, Ashton B, Middleton J: Mesenchymal stem cells: their phenotype, differentiation capacity, immunological features, and potential for homing Stem Cells 2007, 25:2739-2749 57 Tavian ... G, Foroni L, Ventura C, Grossi A, Bagnara GP: Term amniotic membrane is a high throughput source for multipotent mesenchymal stem cells with the ability to differentiate into endothelial cells...

Ngày tải lên: 13/08/2014, 01:20

... Hours - hr Human- specific vimentin - hVIM Human embryonic stem cells - hESCs Human ESC-derived chondrogenic cell-engineered cartilage - HCCEC Human palate mesenchymal cell line - HPM Human umbilical ... populations of mesenchymal stem cells (MSCs) or mesenchymal cells from ESCs to initiate chondrogenic differentiation Different strategies have been employed to induce chondrogenic differentiation ... cells, by centrifugation to form EBs In doing so, a reproducible and robust platform based on hESC differentiation through EB formation was set up for analysis of growth factor influence on differentiation...

Ngày tải lên: 11/09/2015, 09:57

... Stem cells 1.5.1.1 Embryonic stem cells 1.5.1.2 ES cell stem therapy is not ideal for clinical use 1.5.2 Adult stem cells 10 1.5.2.1 Bone marrow stem cells ... Bone marrow stem cells There are most common types of stem cells in bone marrow: (i) hematopoietic stem cells and (ii) mesenchymal stem cells These cell types are believed to be ideal for myocardial ... has since been believed that hematopoietic stem cells may not differentiate into cardiomyocytes45 1.5.2.1.2 Mesenchymal stem cells Mesenchymal stem cells (MSCs) encompass a heterogeneous population...

Ngày tải lên: 12/09/2015, 09:42

... adult human mesenchymal stem cells Science 284, 143–147 Porada CD, Zanjani ED & Almeida-Porad G (2006) Adult mesenchymal stem cells: a pluripotent population with multiple applications Curr Stem ... marrowderived mesenchymal stem cells and mononuclear cells Stem Cells 25, 1166–1177 Li L, Zhang S, Zhang Y, Yu B, Xu Y & Guan Z (2009) Paracrine action mediates the antifibrotic effect of transplanted mesenchymal ... to hypoxia ⁄ SD for h The cells were then fixed in 2% paraformaldehyde in NaCl ⁄ Pi for 30 min, washed twice with NaCl ⁄ Pi and permeabilized with 0.3% Triton X-100 in NaCl ⁄ Pi for 10 Next, the...

Ngày tải lên: 18/02/2014, 04:20

... Guidelines for Human Embryonic Stem Cell Research http://www.nap.edu/catalog/11278.html GUIDELINES FOR HUMAN EMBRYONIC STEM CELL RESEARCH Committee on Guidelines for Human Embryonic Stem Cell ... with human cells was significant because of its implications for improved health The dual capacity of hES cells for self-renewal and for differentiation into repair cells offers great potential for ... for Human Embryonic Stem Cell Research http://www.nap.edu/catalog/11278.html Introduction Stem cells are capable of self-renewal and also of differentiation into specialized cells Some stem cells...

Ngày tải lên: 06/03/2014, 15:20

... been reported in human [16,17] and mouse embryonic stem cells [18-20] The unique patterns of miRNA expression in embryonic stem cells suggest they are involved in maintaining "stemness" Identifying ... were upregulated in adult cells HMVEC = human microvascular endothelial cells, HUVEC = human umbilical vein endothelial cells, UASMC = umbilical artery smooth muscle cells; NHA = normal astrocyte ... been reported in human embryonic stem (ES) cells [16,26], mouse ES cells[ 20] or Page 12 of 17 (page number not for citation purposes) Journal of Translational Medicine 2009, 7:20 human EB [17] depending...

Ngày tải lên: 18/06/2014, 15:20

... CA) Unstained cells were gated on forward scatter to eliminate particulate debris and clumped cells A minimum of 5.000 events were counted for each sample Mesenchymal Stem Cell Differentiation ... MO) for minutes Counterstaining was finally performed with Van Gieson [14] The calcium accumulation was indicated by dark color Myogenic Differentiation For myogenic differentiation hFTs cells ... multilineage differentiation was performed for independent lineages of htMSCs, and no evident difference in their differentiation potential was observed between them In addition, the potential for hFTs cells...

Ngày tải lên: 18/06/2014, 15:20