docosahexaenoic acid signalolipidomics in the homeostatic modulation of photoreceptor retinal pigment epithelial cell integrity during oxidative stress
Báo cáo khoa học: Identification of an antibacterial protein as L-amino acid oxidase in the skin mucus of rockfish Sebastes schlegeli pot
Ngày tải lên :
30/03/2014, 10:20
... glycoprotein consisting of 75-kDa subunits by SDS ⁄ PAGE, partly because of the scarcity of the protein of interest In this study therefore we isolated the antibacterial protein, SSAP, from the skin ... superfamily lectins via its sialylated glycan moiety results in the production of locally high concentrations of H2O2 in or near the binding interface The role of the glycosyl substituents of LAOs in biological ... al [35] examined the structure of the glycan moiety of the LAO from the Malayan pit viper, Calloselasma rhodostoma, and assumed that putative binding of the LAO to sialic acid- binding Ig superfamily...
- 12
- 470
- 0
Báo cáo y học: " Conserved charged amino acid residues in the extracellular region of sodium/iodide symporter are critical for iodide transport activity" potx
Ngày tải lên :
10/08/2014, 05:21
... exchanger [19-23] Therefore, herein we analyzed the critical roles of amino acid residues in the extracellular segments of NIS, and our findings indicated that these residues affected the iodide transport ... at these residues affected the Km values, suggesting that these amino acid residues might influence the binding of iodide ions Lys-86, His-226, and Arg-228 were situated on the other side of the ... resulted from the amino acid substitution instead of transfection variation These findings suggested that conserved charged amino acid residues, except Arg-9, in the extracellular region of NIS were...
- 9
- 398
- 0
Báo cáo y học: "Amino acid substitutions in the E2 glycoprotein of Sindbis-like virus XJ-160 confer the ability to undergo heparan sulfate-dependent infection of mouse embryonic fibroblasts" docx
Ngày tải lên :
12/08/2014, 01:21
... HSdependent in infection of cells due to lacking of the two basic amino acids in the second HS-binding domain Specific interaction of the peptide containing 145-150 aa from YN87448 E2 gene with heparin ... amino acid is part of a binding site for HS or even that the amino acid is exposed on the surface of the virion However, together with the notion that binding sites of SINV to HS are composed of ... of the linear HS-binding domains and the scattered positively charged aa [16], our data demonstrate the importance of positively charged residues in the HS-binding domain of E2 in mediating SINV...
- 6
- 376
- 0
Tài liệu Báo cáo khoa học: Identification of different isoforms of eEF1A in the nuclear fraction of human T-lymphoblastic cancer cell line specifically binding to aptameric cytotoxic GT oligomers ppt
Ngày tải lên :
21/02/2014, 00:20
... protein belonging to the GTPbinding elongation factor family, which promotes the GTPdependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis and the capture of ... activity of cancer cells One hypothesis may be that these oligomers exert their action by blocking the binding of eEF1A to its ligand in cancer cells, perhaps to zinc finger proteins involved in the modulation ... basic proteins it might be that either methylation of the lysine or specific substitutions in the amino acid sequence, increasing the number of basic residues of lysine, arginine and histidine, could...
- 12
- 552
- 0
Báo cáo y học: "Systems biology-defined NF-κB regulons, interacting signal pathways and networks are implicated in the malignant phenotype of head and neck cancer cell lines differing in p53 status" pps
Ngày tải lên :
14/08/2014, 08:20
... dominantly associated with components of the neuregulin signaling pathway (the third row) In the remaining signaling and functional pathways, with the exception of cell cycle:G2/M checkpoint ... involved in the binding of the IL8 promoter, leading to target gene expression [6,14] Next, we tested the binding activity on the promoters of less studied NF-κB targeted genes The promoter of ... the down-regulation of these genes has been shown to be the critical step in tumorigenesis in epidermis and epithelia [76] Interestingly, the involvement of RELA and NFκB1 in the Wnt/β-catenin...
- 22
- 434
- 0
Báo cáo y học: Esterified Hyaluronic Acid and Autologous Bone in the Surgical Correction of the Infra-Bone Defects"
Ngày tải lên :
03/11/2012, 11:35
... [14-16] Interactions between basal epithelial cells and the extracellular matrix are mediated by special receptors on the cell surface which are known as integrins and belong to the family of cellular ... suggest that integrin-mediated interaction plays a decisive role in 67 the regulation of proliferation, migration, and differentiation of the epithelial cells [2,12,18,19] Following the surgical ... recession, i.e the position of the gingival margin with respect to the cement enamel junction (CEJ); • CAL (clinical attachment level): i.e the position of the attachment in relationship to the CEJ;...
- 7
- 769
- 0
Tài liệu Báo cáo khoa học: A novel coupled enzyme assay reveals an enzyme responsible for the deamination of a chemically unstable intermediate in the metabolic pathway of 4-amino-3-hydroxybenzoic acid inBordetellasp. strain 10d doc
Ngày tải lên :
19/02/2014, 16:20
... Enlargement of the original plots shown in (B) present in cell extracts of succinate/glucose-grown cells; therefore, the synthesis of the enzyme was inducible Table shows a summary of a typical ... resting cells of a mutant, strain Y-2, of the aniline-assimilating Pseudomonas sp strain AW-2 [20] Results Spectral changes during metabolism of 4-amino-3hydroxybenzoic acid by crude extracts of ... analysis, and the determination of released ammonia The coupled enzyme assay revealed the mechanism of the deamination reaction and the subsequent metabolism, including the deamination step The product...
- 7
- 613
- 1
Báo cáo khoa học: Glutamic acid residues in the C-terminal extension of small heat shock protein 25 are critical for structural and functional integrity pptx
Ngày tải lên :
07/03/2014, 04:20
... Frequency of amino acid residues in the flexible region of the C-terminal extensions of human and murine sHsps Residues present in the flexible region of the C-terminal extension of each of the eight ... protein indicates that the structure of the N-terminal domain is maintained, at least in the vicinity of the tryptophan residues The increase in exposed clustered hydrophobicity observed for the ... [17–20] The drastically reduced thermostability of the glutamic acid residue mutants demonstrates the importance of each of the negatively charged residues in the C-terminal extension in maintaining...
- 14
- 417
- 0
Báo cáo khoa học: Stage specific expression of poly(malic acid)-affiliated genes in the life cycle of Physarum polycephalum Spherulin 3b and polymalatase potx
Ngày tải lên :
07/03/2014, 12:20
... spherulin 3a and NKA48 contain only a single domain fold The stability of these two proteins is highly dependent on calcium binding [25] The typical domain motives contain a ‘tyrosine corner’ in the ... result of loss of induction at the transcriptional level, of loss of activation of the synthetase protein itself, or of derepression of enzyme(s) catalysing PMLA degradation An interesting interplay ... express a distinct fragmin-like actin-binding Cell type expression of spherulin 3b and polymalatase protein that controls in vivo phosphorylation of actin by the actin-fragmin kinase Eur J Biochem...
- 10
- 639
- 0
Báo cáo khoa học: Structural and functional consequences of single amino acid substitutions in the pyrimidine base binding pocket of Escherichia coli CMP kinase pdf
Ngày tải lên :
16/03/2014, 10:20
... with the amino group and the N3 atom of the cytosine (Fig 1) This study uses site-directed mutagenesis to further explore the contribution of these amino acids interacting with the pyrimidine ring ... CMP The side chain of R110 is a hydrogen bond donor to the N3 atom of cytosine As the main chain carbonyl of D129, a H-bond acceptor, interacts with the terminal oxygen of S36 side chain, the ... energies of the original resiDG i;j due i in the native protein with the rest of the residues, self DDG k mutị are the changes of the self energies of the residues k that change either their ionization...
- 11
- 437
- 0
Báo cáo Y học: Role of tyrosine 238 in the active site of Rhodotorula gracilis D-amino acid oxidase A site-directed mutagenesis study docx
Ngày tải lên :
17/03/2014, 17:20
... following the binding of anthranilate (Fig 3, inset) and the shoulder at % 500 nm following the binding of benzoate (De497nm of 7500ặM)1ặcm)1 vs a gure of 2000 4000ặM)1ặcm)1 observed with the other ... in binding were observed for Y238 mutants with the ligands tested (Table 2) These results indicate that the mode of ligand binding is retained in the two mutants, and that the alteration of the ... of the oxidized enzyme was converted into the reduced form, lacking the isosbestic points and peak maxima characteristic of the formation of the semiquinone intermediate The amount of semiquinone...
- 10
- 496
- 0
Báo cáo khoa học: New members of the brachyurins family in lobster include a trypsin-like enzyme with amino acid substitutions in the substrate-binding pocket potx
Ngày tải lên :
23/03/2014, 03:20
... amino 15 15 15 15 15 acids acids acids acids acids amino amino amino amino amino acids ⁄ 237 acids ⁄ 237 acids ⁄ 237 acids ⁄ 237 acids ⁄ 249 amino amino amino amino amino 266 266 266 266 278 AATAAA ... chymotrypsin numbering) is involved in the formation of a salt bridge with the N-terminal Ile of the mature enzyme for completing the formation of Fig Distribution of charged amino acids in Panulirus ... Obtaining 5¢ and 3¢ ends by Rapid Amplification of cDNA Ends (RACE) amino amino amino amino amino acids acids acids acids acids Relative molecular mass ⁄ pI of mature protein homology search in...
- 13
- 474
- 0
Báo cáo y học: "Lack of association of a variable number of aspartic acid residues in the asporin gene with osteoarthritis susceptibility: case-control studies in Spanish Caucasians" potx
Ngày tải lên :
09/08/2014, 07:20
... Program for the Training of University Professors of the Spanish Ministry of Education AG was supported by the Instituto de Salud Carlos III (Spain) References Our results were similar to the UK study ... involving differences in disease phenotype, in lifestyle, and in environmental and genetic factors Conclusion There was no detectable effect of variation in the number of D residues in ASPN in ... frequency of the D14 allele in patients The same pattern was found when this analysis was repeated after stratifying by sex In the same way, the frequency of the D13 allele was similar in all patient...
- 4
- 431
- 0
Báo cáo y học: " Role of taurine on acid secretion in the rat stomach" ppsx
Ngày tải lên :
10/08/2014, 05:21
... CSAD- and taurine-positive cells We found the presence of taurine-containing cells and taurine-induced acid secretion in the stomach In the body of the stomach, taurine-immunoreactive cells were ... various doses of taurine and acid secretion Values are the mean ± SEM (n = 6) increases the level of cAMP Fourth, the presence of taurine-containing cells in the rat stomach is confirmed, as indicated ... neurons in the muscle layer of the gastrointestinal (GI) tract might be involved in motility of the GI tract and the function of endocrine cells as well Taurine at 10-6 M markedly stimulated acid...
- 10
- 490
- 1
Báo cáo y học: " Modulation of the virus-receptor interaction by mutations in the V5 loop of feline immunodeficiency virus (FIV) following in vivo escape from neutralising antibody" ppsx
Ngày tải lên :
12/08/2014, 23:23
... model of the FIV SU and TM proteins after Pancino et al[67], illustrating the locations of variations in the amino acid sequence of the A613 variants Mutated amino acids are circled (red) with individual ... examined The mutated GL8 clone ABSN retained the neutralisation sensitivity of the parent clone to the A613 plasma, confirming the integrity of the amino acid sequence post-mutagenesis (Fig 6) In ... that the Env-sFcCD134 interaction itself determines the degree of inhibition and that within the range examined, the level of surface CD134 expression on the target cell does not influence the...
- 13
- 344
- 0
Báo cáo y học: " Second site escape of a T20-dependent HIV-1 variant by a single amino acid change in the CD4 binding region of the envelope glycoprotein" doc
Ngày tải lên :
13/08/2014, 09:20
... replication of the wild-type was strongly inhibited by T20 Introduction of the positive arginine (G431R) resulted in increased replication of the wild-type in the absence of T20 This may be due to the ... domain Binding of the surface subunit gp120 to CD4 and a co-receptor on the T -cell surface triggers conformational changes in the Env complex, leading to the insertion of the hydrophobic N-terminal ... engagement, amino acid changes in this region may affect the ability of Env to interact with CD4 and/or co-receptor and thereby influence the cell- cell and virus -cell interactions [32-36] The X-ray...
- 11
- 364
- 0
Báo cáo khoa học: " Expression of cell surface receptors and oxidative metabolism modulation in the clinical continuum of sepsis" ppt
Ngày tải lên :
13/08/2014, 10:20
... neutrophil adaptation across the continuum of sepsis This is a single time point study where the dynamic of sepsis is pointed out by the inclusion of patients at different stages of the disease; sepsis, ... that they have no competing interests 17 Authors' contributions PSM and MKB participated in the design of the study, laboratory tests, and in the writing of the manuscript LSWM performed the laboratory ... with regard to the production of inflammatory cytokines [25] In the present study, we evaluated the expression of TLR2, TLR4, CD11b, CD11c and CD66b on the cell surface of neutrophils in patients...
- 10
- 456
- 0
foreign languages in the labor market of vietnam demands, supplies and recommendation
Ngày tải lên :
06/11/2012, 15:36
... task, then it is not only confined to the Ministry of Labour or Ministry of Education and Training, it is also concerned with human resource planning in the Ministry of Planning or Ministry of Finance ... and teaching has helped Vietnam in the new era of integration On the other hand, the learning and teaching of English at the expense of other languages is posing serious issues We should first ... what are their education and training levels etc It is largely concerned in determining what training needs there are so that the labour supply can be shaped to meet the demands of the economy...
- 32
- 682
- 1
LINH DAM NEW TOWN - SOLUTION FOR THE HIGH-DENSITY DEVELOPMENT OF NEW SETTLEMENTS IN THE SOUTH-WEST OF HANOI
Ngày tải lên :
29/08/2013, 08:15
... the central area for the purpose of resettlement Generally, getting along with the rapid expansion, the east bank of the Red River exhibited the beginnings of urbanization The south-west of the ... components: Bac Linh Dam (North of Linh Dam), Linh Dam peninsula in the middle and Linh Dam expansion (South of Linh Dam) The success of the project has set the stage for a series of New Towns that ... tropical climate Among these, the outstanding point is the area of highstorey apartments and office buildings in the center of Linh Dam peninsula, surrounding by low-storey buildings, parks, open...
- 10
- 805
- 3
LINH DAM NEW TOWN - SOLUTION FOR THE HIGH-DENSITY DEVELOPMENT OF NEW SETTLEMENTS IN THE SOUTH-WEST OF HANOI
Ngày tải lên :
29/08/2013, 08:15
... nặng nhọc với mức thu nhập thấp, kéo theo điều kiện sống mức tối thiểu, tạm bợ khu nhà trọ rẻ tiền với điều kiện sinh hoạt an ninh không đảm bảo Đời sống tinh thần lao động hạn chế Họ thấy cô ... làm việc chợ lao động thuê trọ theo ngày (đêm), lại thuê nhà theo tháng Có khoảng 22,86% nữ lao động tự phải sống nơi có điều kiện sinh hoạt không đảm bảo, vệ sinh; 40,95% không sử dụng nước ... vật chất, tinh thần lao động nghèo nàn Họ thường tiêu tiết kiệm cho khoản chi phí sinh hoạt thiết yếu Có 22,86% nữ lao động tự phải sống nơi có điều kiện sinh hoạt thiếu thốn, vệ sinh Họ thường...
- 8
- 654
- 0