dna sirna nanoparticles for gene therapy

... replication [31], the indispensable DNA polymerases for DNA replication Page of 11 are ideal targets for gene therapy DNA polymerases α, δ and ε that are responsible for DNA replication were chosen as ... systemic chemotherapy [1,3-5] As a new form for cancer treatment, gene therapy has been used for certain cancers, and a number of clinical trials including phase I, II and III trials for various ... Novel approaches, especially for targeted therapy, are being extensively explored Gene therapy is ideal for such purpose for its specific expression of exogenous genes in HCC cells driven by

Ngày tải lên: 29/09/2020, 16:17

... serve as "Trojan horses" for the delivery of genes for cancer therapy. Indeed, clostridial spores that were genetically manipulated to harbour genes for cancerstatic factors, prodrug ... consist of sev- eral aspects: one is from its transgene that encodes prod- rug converting enzymes for suicide -gene therapy or cytokines for immuno -gene therapy. These are essentially the same as the ... larger DNA fragments, but in case the foreign gene is integrated in the host chromosome there is in fact unlimited capacity for insertion of therapeutic genes, forecasting the promising future for

Ngày tải lên: 14/08/2014, 19:22

... glycol) Nanoparticles for Gene Therapy: An Application for pDNA and siRNA Delivery Pharmaceutical Research 27, 2544-2555 (2010) 14 Kievit, F.M et al PEI–PEG–Chitosan-Copolymer-Coated Iron Oxide Nanoparticles ... progress of gene therapy from the bench to bedside like the safe delivery of genes, tracking the gene delivery process and controlled expression of the delivered genes Delivery of genes remains ... 2.1 Nanoparticles for gene delivery and tracking 2.1.1 Polymeric nanoparticles 2.1.2 Metallic Nanoparticles 2.1.3 Quantum dots 10 2.1.4 Rare earth nanoparticles

Ngày tải lên: 09/09/2015, 17:55

... image of siRNA Lane 1: UCN-Ab -siRNA nanoparticles, lane 2: first supernatant (S1) of UCN-Ab -siRNA nanoparticles, lane 3: second supernatant (S2) of UCN-Ab -siRNA nanoparticles, ... lane2: siRNA control; lane3: free siRNA in UCN /siRNA- BOBO3 complex solution; lane4: free siRNA in the solution of UCN /siRNA- BOBO3 added with NaOH 113 Figure 5.6 Biostability of siRNA ... for the fluorescent detection of biological molecules in the buffer For example, Corstjens and his... of siRNA Lane 1: DNA ladder; lane 2: free siRNA control; lane 3: free siRNA

Ngày tải lên: 14/09/2015, 08:39

... strength in the buffer weakened the DNA binding ability of the micelles probably because ions could compete either with DNA for the micelles or with the micelles for DNA P(MDS-co-CES) and P(MDA-co-CEA) ... with the presence of serum This is significant for the gene transfection, 5.1.6 In vitro and in vivo gene expression In vitro gene transfection was performed in various cell lines and primary human ... surface is crucial for the high gene transfection level of the polymer Furthermore, the results of GFP gene transfection performed in different cell lines also indicate that the high gene transfections

Ngày tải lên: 16/09/2015, 15:54

... used as reference. The detection wavelength was set at 318 nm for indomethacin, 273 nm for pyrene, 266 nm for paclitaxel, and 277nm for verapamil. The standard curves were obtained by preparing ... at 100 V for 90 minutes. The naked DNA diluted with the same buffer without adding the micelles and the micelles without adding 53 DNA were used as the controls. The tracks of the DNA and complexes ... containing 40 µg of DNA. The mixture was then vortexed for minutes before analysis of particle size and zeta potential. 3.6.2 Structural integrity of drug-loaded micelles after DNA binding It is

Ngày tải lên: 16/09/2015, 15:54

... of using gene therapy for treating cancers. Several strategies have been proposed for manipulation of gene expression either on the transcriptional or translational level. A deficient gene can ... polymeric micelle formation Formation mechanism and properties of polymeric micelles have been well studied. Micelle formation is driven by two opposing forces including an attractive force between ... gene can be replaced, and the effect of an unwanted gene can be blocked by the introduction of a counteracting gene. For instance suicide gene therapy offers the perspective to kill cancer cells

Ngày tải lên: 16/09/2015, 15:54

... are suicide gene therapy, genetic immunotherapy, tumor suppressor gene or oncogene approaches, and anti-angiogenesis gene therapy. -3- Chapter One: Introduction Suicide gene therapy is one ... al., 2005), among which are suicide gene therapy, genetic immunotherapy, tumor suppressor gene or oncogene approaches, and anti-angiogenesis gene therapy -3- Chapter... an effective ... vector for gene therapy It is impossible to obtain success in gene therapy without effective gene delivery systems that can achieve high levels of therapeutic gene expression in targeted cells. Gene

Ngày tải lên: 01/10/2015, 11:39

... GUIDANCE FOR INDUSTRY:1 FDA GUIDANCE FOR HUMAN SOMATIC CELL THERAPY AND GENE THERAPY OVERVIEW (1998) Since the issuance of the "Points to Consider (PTC) in Human Somatic Cell Therapy and Gene Therapy" in ... Guidance for Industry Guidance for Human Somatic Cell Therapy and Gene Therapy Comments and suggestions regarding this document may be submitted at anytime to Dano B Murphy, HFM-17, Center for Biologics ... vivo by gene therapy given directly to the subject When the genetic manipulation is performed ex vivo on cells which are then administered to the patient, this is also a form of somatic cell therapy

Ngày tải lên: 24/10/2019, 10:26

... strategies for gene delivery is the use of CDcompressed pDNA, which can enhance gene transfection up to 104-fold over naked DNA delivery [25] Generally, in a simple method for preparing pDNA loaded ... be effective for genome editing [1] Many additional gene therapy systems are currently under clinical trials or preclinical evaluation [2] The success of gene therapy relies on the generation of ... liposomal formulations can be used for tumour angiogenesis imaging [96] Recently, Wu et al [97] developed CDs to encapsulate siRNA for imagingguided lung cancer therapy The theranostic CDs absorb

Ngày tải lên: 13/01/2020, 05:03

... gelatin nanoparticles, but also show that these nanoparticles could be used for delivery of gemcitabine in vitro and in vivo, thus allowing the possible combination of gene and drug therapy against ... be actively pursued to improve the performance of the formulation Cytotoxic drug administration in combination with gene therapy is a promising approach for the treatment of pancreatic cancer ... be consistent with that observed for p53 gene loaded nanoparticles The average particle size was found to be between 130-230 nm and the average surface charge for all the different nanoparticle

Ngày tải lên: 05/11/2020, 01:07

... gene therapy 14 2.1.2 Hurdles for gene therapy 15 2.1.3 Systems for gene delivery 16 2.2 Viral vectors for gene delivery…………………………………………… 17 2.3 Non-viral vectors for ... our lab to the gene delivery system 12 Chapter Literature Review 2.1 Gene therapy 2.1.1 Overview of gene therapy Gene therapy is a rapidly advancing field with great potential for the treatment ... targeting problems[57] 2.1.2Hurdles for gene delivery The first challenging hurdle for gene therapy is the short-lived nature of gene therapy The therapeutic DNA introduced into target cells must...

Ngày tải lên: 05/10/2015, 21:33

... vectors for gene therapy formed by self-assembly of DNA with synthetic block co-polymers Hum Gene Ther 7, 2123–2133 11 Kabanov, A V and Kabanov, V A (1995) DNA complexes with polycations for the ... affects the DNA conformation in the complex, allowing regulation of DNA compaction The comb-type copolymers with a higher degree of grafting induce little compaction of DNA and stabilize DNA duplexes ... Vectors for Gene Therapy Edited by: M A Findeis © Humana Press Inc., Totowa, NJ Maruyama and Takei lized by SECs The authors are therefore interested in HA as the ligand to deliver the DNA to...

Ngày tải lên: 11/04/2014, 09:59

... paper) Gene therapy Laboratory manuals Genetic vectors–Laboratory manuals Transfection–Laboratory manuals Viral genetics–Laboratory manuals I Machida, Curtis A II Series [DNLM: Genetic Vectors Gene ... vectors for gene transfer and gene therapy I would like to thank the many chapter authors for their contributions They are all experts in various aspects of viral vectors, and I appreciate their efforts ... Type 1-Based Vectors for Gene Delivery to Human Hematopoietic Stem Cells Ali Ramezani and Robert G Hawley 467 Semliki Forest Viral Vectors for Gene Transfer Jarmo Wahlfors and Richard A Morgan...

Ngày tải lên: 11/04/2014, 10:39

... retroviral genes Oncogene capture The mechanism of oncogene capture appears to be dependent on the generation of a chimeric retroviraloncogene transcript (69, 70) This suggests that the risk of oncogene ... or necessity? Insertional mutagenesis in gene therapy and its consequences Mol Ther 2004, 9:5-13 Dave UP, Jenkins NA, Copeland NG: Gene therapy insertional mutagenesis insights Science 2004, ... in activation of an oncogene, in combination with the requirement for other genetic changes before a cancer eventuates Although proviral integration can also result in gene inactivation, inactivation...

Ngày tải lên: 14/08/2014, 19:22

... of a foreign gene, EGFP under the transcriptional control of the HCMV major immediate early (MIE) promoter was used Page of 11 (page number not for citation purposes) Genetic Vaccines and Therapy ... demonstrates that a foreign gene can be expressed in the context of a HCMV amplicon viral stock in infected HF cells To test the utility of the HCMV amplicon in gene therapy or gene delivery, we ... advantage for the system is that vector DNA could possibly be maintained as an episome with minimal concern for the potential consequences of random integration of vector DNA (i.e activation of oncogenes...

Ngày tải lên: 14/08/2014, 19:22

... carry DNA for gene therapy [9,14,15] For example, a liner PEI-cholesterol conjugation was encapsulated with interleukin-12 to treat RCC mice intravenously, which was demonstrated to be effective for ... as follows: first cycle at 95°C for min, and then 30 cycles at 94°C for 45 s, 54°C for min, 72°C for and a final extension cycle of 72°C for The house-keeping gene b-actin was taken as a loading ... delivery vectors, have already been extensively used to carry DNA for gene therapy For example, the intraperitoneal injection of DNA: PEI complexes is a Table The particle size and zeta potential...

Ngày tải lên: 18/06/2014, 19:20

... carry DNA for gene therapy [9,14,15] For example, a liner PEI-cholesterol conjugation was encapsulated with interleukin-12 to treat RCC mice intravenously, which was demonstrated to be effective for ... as follows: first cycle at 95°C for min, and then 30 cycles at 94°C for 45 s, 54°C for min, 72°C for and a final extension cycle of 72°C for The house-keeping gene b-actin was taken as a loading ... delivery vectors, have already been extensively used to carry DNA for gene therapy For example, the intraperitoneal injection of DNA: PEI complexes is a Table The particle size and zeta potential...

Ngày tải lên: 20/06/2014, 03:20

... PROGENITOR CELLS AS TARGETS FOR GENE THERAPY 143 144 GENE THERAPY FOR HEMATOLOGICAL DISORDERS restoration of cellular and humoral immunity were documented after gene therapy Data showed a surprisingly ... otherwise standard MuLV vectors for gene therapy has not been successful Beyond these HEMATOPOIETIC STEM AND PROGENITOR CELLS AS TARGETS FOR GENE THERAPY 137 efforts, there are obviously major ... In vivo gene transfer is most appropriate for target cells that cannot REQUIREMENTS FOR GENE TRANSFER INTO HEMATOPOIETIC CELLS 135 TABLE 6.1 Relevant Targets and Applications for Gene Therapy...

Ngày tải lên: 10/04/2014, 22:10

... hand, it is necessary for any vehicle used for in 156 GENE THERAPY FOR LIVER DISEASE vivo hepatic gene therapy to reach the liver efficiently For systemic application, the gene vectors are ideally ... attempted so far GENERAL PRINCIPLES FOR HEPATIC GENE THERAPY There are two basic approaches for gene transfer into hepatocytes: ex vivo and in vivo strategies (Fig 7.1) Ex vivo therapy requires ... expression of the therapeutic gene. Alternatively, different viral vectors as well as liposomes can be used for ex vivo gene transfer GENERAL PRINCIPLES FOR HEPATIC GENE THERAPY 155 Collagenase treatment...

Ngày tải lên: 10/04/2014, 22:11