dna recovery of lambda bacteriophage and measurement of mutant frequency

... BCGin /DNA vs BCGsc, DNA- HSP65 and BCGsc /DNA (D) ᭜ BCGin, DNA- HSP65 and BCGin /DNA vs Infected * BCGin /DNA vs BCGsc, DNA- HSP65 and BCGsc /DNA # BCGin vs BCGsc (E) ᭜ BCGsc, DNA- HSP65, BCGin /DNA and ... BCGin /DNA vs BCGin, BCGsc, DNA- HSP65 and BCGsc /DNA (B) ᭜ all immunized-mice vs Infected * BCGin /DNA vs BCGsc, BCGin, DNA- HSP65 and BCGsc /DNA (C) ᭜ BCGin, DNAHSP65, BCGin /DNA and BCGsc /DNA vs ... BCGin /DNA and BCGsc /DNA vs Infected * BCGin /DNA vs BCGsc, BCGin and DNA- HSP65 (F) ᭜ BCGin, DNA- HSP65, BCGin /DNA and BCGsc /DNA vs Infected.* BCGin /DNA vs BCGsc, BCGin, DNA- HSP65 and BCGsc/ DNA p < 0.05...

Ngày tải lên: 14/08/2014, 19:22

... prepared by mixing 0.5 lg of luciferase construct and 0.25 lg of pCH110 plasmid diluted in 30 lL of serum-free medium and lL of SuperFect reagent (Qiagen) per well After 5–10 of incubation at room ... activation of pig endothelial cells and cDNA synthesis pPAEC and PEC-A cells were cultured as described above and were stimulated with 100 UÆmL)1 of recombinant human TNFa (hTNFa; CLB, the Netherlands) ... p10), a1,3GalT transcripts 5¢-A (p11 and p12), 5¢-B (p13 and p14), 5¢-C (p15 and p16), and total amount of pig a1,3GalT mRNA (p17 and p18) were used with cDNA from pPAECs or PEC-A cells (either...

Ngày tải lên: 08/03/2014, 16:20

... TNF-α decreases expression of aggrecan and type II collagen genes and upregulates mRNA expression of matrix metalloproteinase (MMP)-1, -3 and -13 and ADAM-TS4 (a disintegrin and metalloproteinase ... downregulation of aggrecan and collagen types I and II [7] In this study, we have expanded the investigation to include the effect of TNF-α on matrix and MMP gene expression in normal and degenerate ... expression of IL-1Ra can reduce the activity of MMP-1, -3, -7 and -13 in tissue explants [6], we investigated the effect of TNF-α stimulation on expression of MMPs from each of these groups and compared...

Ngày tải lên: 09/08/2014, 14:20

... treatment of sepsis and associated diseases Because of this, it is of interest to determine molecular mechanisms and functions of DHEA treatment We therefore investigated the effects of DHEA application ... quotient of the amount of TNF-α mRNA against the amount of mRNA of the housekeeping gene GAPDH * P ≤ 0.05 The data are expressed as mean ± standard error of the mean (b) In lung after 48 and 96 ... quotient of the amount of TNFα mRNA against the amount of mRNA of the housekeeping gene GAPDH * P ≤ 0.05 The data are expressed as mean ± standard error of the mean CLP = cecal ligation and puncture;...

Ngày tải lên: 13/08/2014, 18:22

... kDa band in T cells A Cb-specific antibody anti-Cb(SNO157) (vi) recognized the 47 kDa band in T-cells, and the 40- and 47 kDa protein bands in Jurkat cells (C) Linear depiction of Ca1, Cb1 and ... p50csk [16] and Raf-1 [17] in the early phase of the stimulatory process and regulation of interleukin-2 production through phosphorylation of the nuclear factor of activated T cells [18] and nuclear ... protein bands of 40 and 47 kDa, respectively (Fig 1B) All of these antibodies were raised against regions in the C subunit that are conserved and therefore not differ significantly between the C isoforms...

Ngày tải lên: 16/03/2014, 18:20

... by the addition of ATP and measurement of Rh as a function of time Addition of mm ATP caused marginal increase in the Rh value of complexes where heteroduplex and homoduplex DNA samples showed ... ratio of protein to DNA (0.15 lm each) After establishing the basic system of MutS and MutL and their interaction with DNA, as reflected by FEBS Journal 272 (2005) 6228–6243 ª 2005 FEBS Addition of ... dimers DNA labelling and annealing Labelling of DNA with 32P at 5¢ end was performed as described [38] Complementary strands (see Table 2) were annealed at a : ratio (10 lm of each strand in...

Ngày tải lên: 07/03/2014, 12:20

... (lane 11), and, when anti-Sp3 IgG was used, bands C2 and C3 disappeared and bands SC3a and SC3b appeared (lane 12) Coaddition of the two antibodies resulted in the loss of bands C1, C2, and C3 (lane ... polymorphism of a high (DNASE2*H) and a low (DNASE2*L) DNase II activity allele in man, Yasuda et al [6] found that DNASE2*H has a G residue at nucleotide )75 of the DNase II promoter, whereas DNASE2*L ... 150 ng of expression vector (pPacSp1 or pPacUSp3) was mixed with 50 ng of pDNaseII()249/+72)-Luc, and the total amount of DNA adjusted to 200 ng with pPac0 The DNA was mixed with 0.6 lL of FuGENE...

Ngày tải lên: 23/03/2014, 17:21

... Effect of MTX, CF101 and MTX+ CF101 treatment on exhibition and expression of A3AR in paw and PBMCs from AIA rats Immunohistochemistry staining of paw sections derived from AIA rats revealed the of ... underlying the efficacy of the combined treatment, we first examined the mode of action of MTX It acts as an antagonist of folic acid, subsequently inhibiting the synthesis of purines and pyrimidines ... upregulation of A3AR mRNA and protein was consistently found in nonpigmented ciliary epithelium of eyes of patients with pseudo-exfoliation syndrome, with and without glaucoma, as compared with normal and...

Ngày tải lên: 09/08/2014, 08:23

... to food and water ad libitum Both the housing and experimental use of animals followed the National Institutes of Health guidelines and were approved by the Institutional Animal Care and Use ... WTs and R6/2s indicate saline treatment, and WTc and R6/2c indicate ceftriaxone treatment N refers to number of animals per group Genotype and CAG repeat length We used PCR for genotyping and ... analyses and genotyping, and helped with data interpretation BRM participated in study design, and helped with data collection and analysis GVR conceptualized and designed the study, and revised...

Ngày tải lên: 10/08/2014, 05:21

... Boise LH: Regulation of Bcl-xL: a little bit of this and a little bit of STAT Curr Opin Oncol 2000, 12(6):543-9 49 Downward J: Mechanisms and consequences of activation of protein kinase B/Akt ... transfection cocktail consisting of 0.4 μg DNA and μl lipofectmine was added to cells grown in 24 well plates containing opti-MEM media (GIBCO BRL, Grand Island, NY, USA) After hr, the transfection ... Viability V (% of control) f Bcl-xL/ -actin n (% of control l) 500 * 80 * 60 * 40 20 VIII ( M) 10 10 ETO Figure Activation of PI3K and Akt pathway and its role in FMRP production and cell viability...

Ngày tải lên: 10/08/2014, 05:21

... culture supernatants of HUVEC He et al Journal of Biomedical Science 2011, 18:39 http://www.jbiomedsci.com/content/18/1/39 Page of Figure Tumorigenesis and angiogenesis of BGC cells in nude mice ... tumor tissues of nude mice STC-1 was detected on the membrane of tumor cells (D) Immunohistochemical staining of PCNA in tumor tissues of nude mice PCNA was detected in the nucleus of tumor cells ... http://www.jbiomedsci.com/content/18/1/39 Page of Figure Effects of STC-1 and VEGF on HUVEC cell migration (A) Effects of STC-1 on HUVEC migration HUVEC were seeded in triplicate on inserts, and incubated for 12 h with...

Ngày tải lên: 10/08/2014, 05:21

... were subsequently collected separately in ml of LHC8 medium (GIBO, Grand Island, NY) An aliquot of this was used for cytology and differential cell count and the remainder was processed immediately ... within the linear range of detection The contrast was inverted, the pixel intensity of each band determined, and the background pixel intensity for a negative area of the film of identical size subtracted ... epithelium (Figure 2C) Up-regulation of GAD67 in Large and Small Airway Epithelium of Healthy Smokers Of all of the GABAergic system genes expressed in the large and small airways, only GAD67 was...

Ngày tải lên: 12/08/2014, 11:22

... consisted of 300ng of DNA, 6l of Terminator Ready Reaction Mix containing DNA polymerase, buffer, dideoxynucleotide (ddATP, ddVTP, ddGTP and ddTTP), 2l of 5X sequencing buffer, and 3.2pmol of sequencing ... examination of the likely anti-tumor suppressive role of C/EBPα and its association to clinical outcome and clinical parameters Perhaps, with a better understanding of its role in HCC and the effect of ... 1l of cDNA, 1µl of forward primer (10µM) and 1µM of reverse primer (10M) and 7µl of water per well The multi-well plate was centrifuged at 2500 rpm for before being placed in the rotor of the...

Ngày tải lên: 02/10/2015, 17:14

... interpreting the results of a forced overexpression of UCP3 in any host system EXPERIMENTAL PROCEDURES Cloning of hUCP3 cDNA and construction of the adenovirus shuttle plasmid The hUCP3 cDNA was obtained ... recombination [27] of two plasmids, one containing the right hand portion of the adenoviral vector genome and a lox site, and the other plasmid, pAvhUCP3lx, containing the left portion of the viral ... the oligomycin-resistant portion of respiration reflects the level of proton leak of mitochondria, the low level of respiration in the presence of oligomycin of L6 cells showed that they were...

Ngày tải lên: 21/02/2014, 15:20

... macrophages and lymphocytes are able to produce a higher amount of anandamide and/ or 2-AG [21,23–26] IgE-dependent stimulation of RBL-2H3 cells also leads to the formation of anandamide and of its ... Purification and quantification of endocannabinoids The extraction, purification and quantification of anandamide, 2-AG and PalEtn from immature and mature dendritic cells requires a set of different ... dehydrogenase and FAAH were selected on the basis of the sequence of the FAAH gene (NCBI accession number AF098010) by including the introns 5476–6026 and 6173–6296, and of the sequence of the glyceraldehyde-3-phosphate...

Ngày tải lên: 22/02/2014, 07:20

... constituent of the Tic motor complex (see below) The present minireview provides a short description of recent advances in the understanding of the channel-, motor- and regulatory components of the ... plants of the pea ortholog and main Arabidopsis isoform of Tic20, AtTic20-I, similarly exhibit pronounced chloroplast defects, and attic20-I knockouts were albino even in the youngest 1168 parts of ... function (e.g in ligand-gated and voltage-gated K+ channels) [16], and thus could provide an explanation for the observed channel activity of Tic110, as well as for the binding of transit peptides...

Ngày tải lên: 07/03/2014, 03:20

... therapeutic MMPIs, it is of pivotal importance to understand the structural basis of recognition, binding and cleavage of substrates, as well as the recognition and binding of natural inhibitors ... and disease MMP-2 degradation of TnI is associated with diminished contractive function of the heart [11], MMP-9 degradation of aB-crystallin with multiple sclerosis [12] and MMP-9 cleavage of ... a : stoichiometric ratio and binding constants in the nanomolar range [26] These SIBLINGs and MMPs are also co-expressed and colocalized in salivary glands of humans and mice [27] The interaction...

Ngày tải lên: 15/03/2014, 00:20

... elongation (Fig 3) We will return to the issue of energy demand and the control of elongation below REGULATION OF eEF2 AND eEF2 KINASE BY INSULIN AND OTHER STIMULI Redpath et al [52] showed that, ... raise the cellular levels of phosphorylation of eEF2 [47,50,51] Such treatments inhibit protein synthesis and rates of elongation, and the increased phosphorylation of eEF2 may explain both effects ... and ventricular myocytes [53] This is associated with a decrease in the activity of eEF2 5364 G J Browne and C G Proud (Eur J Biochem 269) Fig Inhibition of eEF2 and elongation by energy demand...

Ngày tải lên: 23/03/2014, 21:20

... declaration of consent of the donor and the unanimous statement of the donor and the recipient made with both of them present confirming that the donation occurs specifically for the recipient and that ... account the existence of control mechanisms, quality certificates, safety standards and the competent legislation of the country of origin or destination ES The import and export of egg cells is not ... 16-17) Of the remaining 22 survey respondents, countries have at some time received imports of sperm donations: Germany - from Denmark, the Netherlands and the US; Finland - from Denmark and the...

Ngày tải lên: 28/03/2014, 16:20

... mg of purified CT-peptide per liter of bacterial culture Effect of the CT-peptide on mPC1 ⁄ enzymatic activity The effect of various concentrations of the purified CT-peptide on the cleavage of ... in the activation of an enzyme In the case of proPC1 ⁄ 3, removal of the various structural and functional domains is a sequential and coordinated event culminating in removal of the CT-peptide ... the regulation of PC1 ⁄ Results and Discussion It has previously been reported that removal of the PC1 ⁄ CT-peptide has a major impact on the enzymatic characteristics of PC1 ⁄ and this concerns...

Ngày tải lên: 30/03/2014, 08:20