dna and oligonucleotide delivery using chitosan as complexing agent
Label free electrochemical DNA and protein detection using ruthenium complexes and functional polyethylenedioxythiophenes
Ngày tải lên :
11/09/2015, 09:07
... nucleotide base strongly binds to its complementary base through hydrogen bonds Vast amount of literature has been published in DNA hybridization detection, and many comprehensive reviews on DNA- based ... protein assays CNTs served as a carrier for enzyme molecules Using alkaline phosphate (ALP)-loaded CNTs to label detection antibody, as low as 500 fg/mL of IgG was detected in a sandwich assay.51 ... with ds -DNA As an example, Figure 2-5 is the overlay of UV-Vis spectra of Ru-PIND-Ru at increasing amount of salmon sperm DNA As shown in the figure, addition of DNA to Ru-PIND-Ru at a DNA base pair/Ru-PIND-Ru...
- 157
- 591
- 0
Curcumin i knoevenagel’s condensates and their schiff’s bases as anticancer agents
Ngày tải lên :
01/07/2014, 13:23
... for grid and docking was done using ADT Docking was performed with AutoDock 4.0 (Scripps Research Institute, USA) considering all the rotatable bonds of ligand as rotatable and DNA as rigid.52 ... the solution was determined spectrometrically The ratio of absorbance at 260 and 280 (P1.8) indicated that DNA was sufficiently free of protein The concentration of DNA was measured using its extinction ... solution was added to 0.2 mL of supernatant and the sample was allowed to stand for 15 The amount of cyanmethemoglobin in the supernatant was measured at 540 nm and compared with standard curve...
- 13
- 569
- 0
Tài liệu Báo cáo khoa học: Identification of Ewing’s sarcoma protein as a G-quadruplex DNA- and RNA-binding protein ppt
Ngày tải lên :
15/02/2014, 01:20
... G-quadruplex DNA (A) EMSA was performed with EWS (lanes and 4) and 32P-labeled ETS-1 (lanes and 4) or ssDNA L (lanes and 2) (B) EMSA was performed with EWS (lanes 2, and 6) and 32P-labeled Htelo (lanes and ... (A) EMSA was performed with RGG3 (lanes and 4) and 32P-labeled Htelo (lanes and 2) or rHtelo (lanes and 4) (B) EMSA was performed with RGG3 (lanes and 4) and 32P-labeled rHtelo (lanes and 2) or ... labeled DNA The DNA protein complexes were resolved by 6% PAGE and visualized by autoradiography (F) DNA polymerase arrest assays Primer extension reactions were performed with rTaq DNA polymerase...
- 11
- 786
- 0
Using lichen as bioindicator of air pollution: Acid deposition Monitoring and Assessment Third country Training pptx
Ngày tải lên :
29/03/2014, 18:20
... the tops of sandstone walls, on asbestos roofs, and on ash trees decline as New Castle is approached from the west (From Gilbert 1965) Sand stone Ash tree Roof Relative cover and biomass of two ... lichen deserts: on asbestos, on sandstone, and on ash trees Ash s tree ne sto nd Sa f Roo pH from bark of ash trees and lichen covers along distance from the city of New Castle to the west Distan ... of two lichen species on ash trees along an east – west transect to the west of New Castle, England (From Gilbert, 1969) Evernia punestri Lecanora conizaoeides cover biomass cover Lichen desert:...
- 23
- 408
- 1
Báo cáo y học: "Assessing post-traumatic stress disorder in South African adolescents: using the child and adolescent trauma survey (CATS) as a screening tool" docx
Ngày tải lên :
08/08/2014, 21:20
... Robertson, Zissis and Leger [11] used self-report measures to determine exposure to violence, as well as structured questionnaires and nonstandardized clinical assessments to elicit symptoms and make ... someone s/he knows well has been badly beaten, or has been kidnapped during the participant's lifetime Participants were also asked to note which was the worst event experienced and to report PTSD ... Stellenbosch and Cape Town Consent from the heads of schools, parent bodies, and parents and learners was also obtained Learners and their parents/guardians were informed that participation was entirely...
- 10
- 667
- 0
Báo cáo sinh học: " A new plasmid vector for DNA delivery using lactococci" pptx
Ngày tải lên :
14/08/2014, 19:22
... safer strategies for DNA deliver and expression into eukaryotic cells, we reinforce the use of Lactococcus lactis as DNA delivery vehicle [18,26] To improve our delivery DNA strategy, we constructed ... lactic acid bacteria are generally regarded as safe they still be viewed as such when invasive L lactis inlA+ survival rate was measured and showed a decrease from 4,5 log CFU/ml for 24 hours after ... lactis, as DNA delivery vehicle at the mucosal level VG and SI performed the experiments of the work VG drafted the manuscript and AM contributed to improve it JMC and FL coordinated it PL, VA and...
- 7
- 313
- 0
Investigation of new properties and applications of quadruplex DNA and development of novel oligonucleotide based topoisomerase i inhibitors
Ngày tải lên :
11/09/2015, 16:04
... oligonucleotide, sequence (b) 79 5-1 Superhelical tension generated by DNA unwinding and resolved by DNA topoisomerases 82 5-2 Type I and Type II DNA topoisomerases 83 5-3 Mode of action of DNA ... molecular level through using three strands of DNA (DNA tweezers, Figure 2-3) In this system, strand A were hybridized to each end of strands B and C to form two arm-like double-stranded structures ... Efficiency of Topoisoemrase I 95 5.3 Gap-Containing Unimolecular Oligonucleotides as Topoisomerase I 98 Inhibitors 5.3.1 Design of Gap-Containing Oligonucleotides as Topoisomerase I Inhibitors 5.3.2...
- 158
- 358
- 0
Investigation on factors affecting drug delivery using polymers and phospholipids 1
Ngày tải lên :
14/09/2015, 14:03
... prolong drug release and decrease physiological toxicity resulting from fast release of drugs A novel anti-wrinkle polymeric nanofiber of PVA and RM β-CD face mask containing ascorbic acid, retinoic ... seconds Polyesters, PLA and PLGA, releases drug via hydrolysis of the polymer and could provide sustained and controlled release rate of the drug Blends of these hydrophilic and hydrophobic polymers ... solubility and complexation energy of haloperidol was studied Highest increase in drug solubility was observed when the drug was in its degree of ionized form in RM β-CD, resulting in a 128-fold increase...
- 15
- 294
- 0
Investigation on factors affecting drug delivery using polymers and phospholipids 2
Ngày tải lên :
14/09/2015, 14:03
... drug delivery is widely used for the treatment of localized skin disease such as acne vulgaris as well as musculoskeletal disorders However, over the past few decades there is an increasing emphasis ... (Tadicherla and Berman 2006; Asbill and Michniak 2000) Polymeric delivery systems can protect the active ingredient from degradation and prolong or modify the drug release pattern Polymers such as chitosan, ... proniosomes was viewed using a CLSM The CLSM was a Nikon A1R laser scanning confocal and digital camera from Japan All samples were excited at 405, 488 and 561 nm and X-Z sectioning has been used...
- 46
- 288
- 0
Investigation on factors affecting drug delivery using polymers and phospholipids 3
Ngày tải lên :
14/09/2015, 14:03
... the HPLC assay 3.2.5 Surface Tension and Contact Angle Measurements Surface tensions of RM β-CD and HP β-CD solutions and each formulation used in the permeation study were measured using the ... substantial increase in HP permeability was obtained by using 0.1% w/v RM β-CD as the receptor solution and RM β-CD 0.01 M in donor compartment (Fig 3.5d) The flux was enhanced by a 1.6fold increase compared ... Biodegradable polymeric nanofibers have been used as carriers for drug delivery and may offer advantages such as reduced toxicity and increased therapeutic level and biocompatibility (Yang et al., 2007;...
- 32
- 267
- 0
Investigation on factors affecting drug delivery using polymers and phospholipids 4
Ngày tải lên :
14/09/2015, 14:03
... (5 mg) was suspended in 10 ml of PBS and was placed in a shaking incubator at 37oC or 20oC Samples of ml were taken from the medium periodically and the released drug was determined using HPLC ... formaldehyde and ml acetic acid (Gaspar and Campos 2007) After a series of dehydration, they were embedded in paraffin and semi-thin sections were cut and stained with hematoxylin and eosin prior ... AA and for RA when compared to the vitamin-loaded cotton face mask (p > 0.05) Presence of gold increases the permeation of ascorbic acid (p > 0.05) Although there was no significant increase...
- 25
- 273
- 0
Investigation on factors affecting drug delivery using polymers and phospholipids 5
Ngày tải lên :
14/09/2015, 14:03
... epidermis and decrease the drug permeation through the skin (Ga de Jalón et al., 2001a and b; Rolland et al., 1993; Tsujimoto et al., 2007) Several studies have shown sustained and controlled release ... confocal study was carried out Skin samples were treated with polymeric microparticles and then aquous solution of 0.03% w/v fluorescein dye was applied and its skin penetration was viewed using a CLSM ... stability and drug release profile (Wischke et al., 2006) Water has a plasticizing effect on polyesters and lowers the Tg from > 40oC to around 37oC (Wischke et al., 2006; Middleton and 113 Tipton...
- 63
- 645
- 0
Identifying determinants of background matching and disruptive colouration using computer simulations and humans as predators
Ngày tải lên :
09/10/2015, 11:06
... complex background as fast as possible Meanwhile, the system randomly assigned a background and morph category for each volunteer, and checked if such combination was previously assigned to less ... × 79 pixels octagon “mask” was first created using ImageJ 1.41 By randomly placing the “mask” onto the background previously generated using Adobe Photoshop CS2, a morph was created by cutting ... simulation was presented to human volunteers as a computer game, in which they were requested to find the morph from the background as fast as possible The program was written in JAVA as a Windows...
- 121
- 426
- 0
Nonviral gene delivery using polymer and peptide to develop safe and cell specific gene vectors
Ngày tải lên :
27/11/2015, 11:11
... washed and permeabilized with the reporter cell lysis buffer (Promega, WI, USA) The luciferase activity in cell extracts was measured using a luciferase assay kit (Promega, WI, USA) Each measurement ... manner with 0.2 µg of plasmid DNA per complex or triplex 3.5 Agarose Gel Electrophoresis Plasmid DNA (0.2 µg) was mixed with Polymer /DNA, peptide /DNA complexes or polymer /DNA/ peptide triplexes ... and purified with HiSpeed Plasmid Kit (Qiagen, Hilden, Germany) The quantity and quality of the purified plasmid DNA was assessed by optical density at 260 and 280 nm and by electrophoresis in...
- 96
- 184
- 0
“Good” Legislation as a Means of Ensuring Voice, Accountability, and the Delivery of Results in Urban Development
Ngày tải lên :
26/08/2016, 07:50
... and the availability and diversity of labor and market size Instead, they generate congestion and high costs for infrastructure and services and cannot support the creation of sufficient jobs and ... renewable, and long-term user right comparable to a lease According to the Urban Land Regulations,14 DUATs in urban areas cannot be issued before the land is provided with urban basic services, and ... simple, understandable, and easy to comply with Complexity increases costs for citizens and public administration, hinders compliance, and ultimately undermines the social contract and the fair...
- 14
- 355
- 0
Báo cáo y học: "Parvovirus B19 Nonstructural Protein-Induced Damage of Cellular DNA and Resultant Apoptosis"
Ngày tải lên :
25/10/2012, 11:18
... phase of the experiment Labeled DNA was detected by autoradiography and proteins were detected by Western blotting Radioactivity was found in specific bands that perfectly overlapped with bands ... apoptosis ATM principally binds to free DNA ends or DNA strand breaks (43), while ATR recognizes single-stranded regions of DNA common to multiple types of DNA lesions and that are often caused by collapsed ... could easily cause double strand breaks through the simple mechanism of nicking both DNA strands a short distance apart Nicking and binding to the DNA end would not only create broken strands,...
- 9
- 469
- 1
Báo cáo y học: "HIV DNA and Dementia in Treatment-Naïve HIV-1-Infected Individuals in Bangkok, Thailand"
Ngày tải lên :
31/10/2012, 15:34
... real-time PCR assays in triplicate using independent standard curves generated to measure relative HIV DNA copy number and cellular equivalent genomic DNA The plasmid used to generate the standard curves ... validated in Thailand [18] The assessment was a clinical assessment made by the protocol neurologist (P S.) at the time of clinical evaluation using the TDI, patient interview, and patient and proxy ... previously reported [15] HIV DNA, normalized to the number of copies of HIV-1 DNA per 106 cells, was then measured using real-time polymerase chain reaction (PCR), as previously described [15]...
- 6
- 541
- 2
USING BRAND AS AN EFFECTIVE WEAPON TO COMPETE IN THE MARKET: A CASE STUDY OF NHAT LINH COMPANY
Ngày tải lên :
13/04/2013, 10:29
... benefits • Brand identity consists of twelve dimensions organized around four perspectives - the brand -as- product, brand -as- organization, brand -as- person, and brand -as- symbol • Brand identity ... purpose and meaning for the brand Aaker (1996) gives a variety of definitions of brand identity such as: • Brand identity is a unique set of brand associations that the brand strategist aspires ... offer quality assurance and reduce risks • Brands help self-expression • Brands offer friendship and pleasure 2.3.2 A brand is more than a product Brand is more than a product Branding is about...
- 67
- 974
- 0