distribution of sodium and potassium between cells and body fluids

... functions, of sPLA 2 -IID and those of sPLA 2 -IIA and -V are not entirely identical and are even cell- and tissue-specific. Searching the nucleic acid database reveals the presence of the TATA box and ... in KE2 and KE2RS2, respectively. (B) Expression of the wild-type (WT) and two mutants of mIID in HEK293 cells, as assessed by RNA blotting. (C) Membrane binding of the WT and two mutants of mIID. ... was detected in KM12, KM20L2 and WiDr cells. COX-1 was highly expressed in HT29 and WiDr cells and weakly expressed in KM20L2 cells. COX-2 was detected only in WiDr cells. The two terminal PGE 2 -biosynthetic...

Ngày tải lên: 18/03/2014, 01:20

Ngày tải lên: 09/08/2014, 09:20

Ngày tải lên: 14/08/2014, 19:22

... isolation and maintenance of murine endothelial cells from various developmental stages and locations is important for dissecting molecular and cellular mechanisms of endo- thelial development and ... concentra- tion of G418 (Invitrogen) of 400 lgặmL )1 . G418-resistant colonies were picked and expanded for PCR genotyping and the formation of embryoid bodies. One ESC clone (T26) out of 48 G418-resistant ... prop- erties of liver sinusoidal ECs in health and disease. Isolation and characterization of BECs and LECs We next isolated LECs from the mixed cell population by magnetic immunosorting using an antibody...

Ngày tải lên: 18/02/2014, 17:20

... asymmetric stretching of CH 3 ,and the band at 2 873 cm )1 to its symmetric mode. T he bands at 2926 and 2853 cm )1 can be a ssigned to an asymmetric and symmetric stretching mode of CH 2 , r espectively ... tool for discrimination between sensitive and multiresistant K562 cells Anthoula Gaigneaux, Jean-Marie Ruysschaert and Erik Goormaghtigh Laboratory of Structure and Function of Biological Membranes, ... variance and the smallest intraclass variance, and constructs a linear combination of the variables to discriminate between the classes. The rule i s constructed with training set of samples, and...

Ngày tải lên: 21/02/2014, 03:20

... only from body parts and tyres of the vehicles. The amount of Zn in the runoff is higher than the amount measured in the soil. This could be because of discharge of some of the runoff waters ... proportion of total metal in the exchangeable fraction whereas Cu is mainly in the strongly bound organic and residual phases. Hewitt and Candy (1990), examined levels of Pb, Cd and Zn in soil and dust ... measurements of lead. Gibson and Farmer (1984) applied a six-step sequential leaching procedure to soil and street dirt in order to under- stand environmental mobility and bioavailability of Pb, Zn, Cu and...

Ngày tải lên: 16/03/2014, 00:25

Ngày tải lên: 16/03/2014, 03:20

... i.e. DIMs and PGL-tb; and (b) to address the question of the function of the two related molecules DIM A and DIM B. According to the accepted model of DIM and PGL biosynthesis [17], DIM A and PGL-tb ... the question of the specific role of DIM A and DIM B in the resistance of cells to SDS. We demonstrated that the occurrence of comparable amounts of DIM B can complement the absence of DIM A with ... Competition between mutant and wild-type strains in infected mice. (A) Numbers of CFUs recovered from the lungs and the spleen of mice infected with a mixture of the wild-type H37Rv:pMV361H and DIM-less...

Ngày tải lên: 23/03/2014, 09:20

... Caco-2 cells, 20 and 50 l M for SKPT cells) in the presence of increasing amounts of alafosfalin. The linear, nonmediated transport components, i.e. simple diffusion plus tracer binding, of 19.2% and ... Transport of the phosphonodipeptide alafosfalin by the H + /peptide cotransporters PEPT1 and PEPT2 in intestinal and renal epithelial cells Jana Neumann 1 , Mandy Bruch 1 , Sabine Gebauer 2 and Matthias ... inhibition constants and the competitive nature of inhibition were confirmed in Dixon-type experiments. Caco- 2 cellsand SKPT cells were also cultured on permeable filters: apical uptake and transepithelial...

Ngày tải lên: 23/03/2014, 12:20

... two bands in cells incubated with recombinant PHB, the top band corresponding to His-tagged PHB (Fig. 5A). These data indicate that exogenous PHB enters the cells. PHB is expressed in b -cells and ... western blot of PHB protein expression (A,B) and caspase-3 (C,D) after siRNA trans- fection in RINm5F (A,C) and INS-1E (B,D) cells. (E) Percentage ratio of float- ing ⁄ attached cells expressed ... to investigate the expression and antioxidant effects of PHB in pancreatic b -cells exposed to ethanol. Results Effects of ethanol and recombinant PHB on b-cell mitochondrial function and apoptosis We first...

Ngày tải lên: 29/03/2014, 08:20

... pre- ceding the a-helix and the C-terminal b-strand of RBP and the hyper-variable regions of Fab: loops 53–56 and 100–103 and the short helix 28–32 of chain H and loops 31–36 and 53–56 of chain L. The ... b-strands H and F. To form the tetramer, two dimers associate back to back, mainly through hydrophobic contacts between residues of the loops formed by b-strands A and B and b-strands G and H. ... b-strands G to F and is flexi- ble in most TTR structures, and for loop 19–22, which connects b-strands A and B and hosts the mutation. The movement of the latter loop is not large (the Ca atom of...

Ngày tải lên: 30/03/2014, 02:20

... limit of detection of NPFF-IR material was estimated to be between 8 and 12 fmol. Analysis of the binding characteristics of the NPFF antiserum indicated that, among all the possible derivatives of ... fmol of synthetic SQA-NPFF (A), at m/z 684.5 from HPLC fractions of NPFF-IR peak 2 (B), at m/z 990.0 from 100 fmol of synthetic hNPAF (D) and at m/z 990.0 from HPLC fractions of NPFF-IR peak3(E)andMS/MSspectraofthe[M+2H] 2+ precursor ... of QFW-NPSF in the sample. The coelution of SPA-NPFF and QFW-NPSF did not allow the quantification of each peptide in the HPLC fractions. Considering the poor affinity of QFW-NPSF for the antibody...

Ngày tải lên: 31/03/2014, 07:20

... return to the books of the ancients and in particular to those of Aristotle, Alexander of Aphrodisias, and al-Fa ¯ ra ¯ bı¯ in philosophy, and to those of Hippocrates and Galen in medicine. During ... presence of Abu ¯ Ja‘far ibn Banawayh (d. 963) the king of Sijista ¯ n and a close friend of al-Sijista ¯ nı¯, excerpts from the cenacles of Abu ¯ l-Fad ˙ l ibn al- ‘Amı¯d and discussions between ... traces of his own understanding of the Imamate theory). Al-Sijista ¯ nı¯ enu- merates the different positions held by the followers of different sects of his times on God and the higher beings, and...

Ngày tải lên: 30/05/2014, 23:48

... coordination, and preparation of this manuscript. SK, MM, AT, EN, and, YN prepared reagents and PBMCs. SH, HK, and YS prepared fusion cells and performed vac- cination of the patient. TO, KF, JG, and HT ... expressed high levels of WT1 and HLA-ABC and low levels of CEA but not HLA-DR, CD80, CD86, and CD83 molecules (Fig- ure 1B, 2A, and 5A). Fusions of DCs to the HCC cells coex- pressed the HCC cells- derived ... expression of maturation molecules of DCs/ tumor fusion cells. Induction of HCC cells- specific CTL by DCs/allo-HCC To determine whether HCC cells- reactive T cells are induced by fusion cells, T cells...

Ngày tải lên: 18/06/2014, 15:20

... DCTs and DCTIs have an optimal antigen uptake capacity and upregulation of CD86, CD40 and class I, but low levels of CD83, as is expected of non-terminally mature DCs. The phenotypic profile of ... or is cryptic on the surface of tumour cells and that immunization of cancer patients with hTERT p540 leads to the production of T cells that do not recognize tumour cells in vivo based on this ... ME: conception and logistics of the study. MMA, CV, SS, RAR, SC AJM: vac- cination of patients, acquisition of samples and genera- tion of data. MMA, AJM, NRH, JB, FF: preparation of the vaccine....

Ngày tải lên: 18/06/2014, 15:20

... Specifically, 10 ul of antibody was added per 100 ul of resuspended cells and refrigerated in the dark for 15 min (4-8) C. Cells were washed in 2 ml of PBS with 0.5% BSA and resuspended in 100 ul of buffer ... a concentration of 10 (6) cells per well. After 5 days of culture cells were Giemsa stained and clusters of > 50 cells were quantified as colonies. Mikirova et al. Journal of Translational ... Pro- ducts, Chandler AZ), was capable of increasing the number of circulating stem cells and progenitor cells. This proprietary food supplement is produced by fer- mentation of a combination of green...

Ngày tải lên: 18/06/2014, 16:20

... Ltd, Japan); 200 cells were counted. T cells sorting The CD4+ and CD8+ T cells from 19 CML cases and 17 healthy individuals were sorted using CD4 and CD8 monoclonal antibody and MACS đ Magnetic ... sjTRECs differed significantly between CML and healthy control in 2 ì 10 5 , 5 ì 10 4 and 1 × 10 4 PBMCs or in 1 × 10 4 of CD4+ and CD8+ T cells (Figure 2). Comparison of the frequencies of 23 TRBV-BD1 sjTRECs ... to the guidelines of the Medical Ethics committees of the health bureau of Guangdong Province of China. sjTRECs were measured in PBMCs from all 48 cases, and CD4+ and CD8+ T cells from 19 cases....

Ngày tải lên: 18/06/2014, 16:20

Ngày tải lên: 20/06/2014, 01:20

Ngày tải lên: 20/06/2014, 08:20

Ngày tải lên: 08/08/2014, 14:21